LOCUS       c00003_gnlPro..        14960 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HLGCADFP_3.
ACCESSION   c00003_gnlPro..
VERSION     c00003_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 09:34:09
            Original ID  :: gnl|Prokka|HLGCADFP_3
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14960
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14960
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[100:4960]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      101..4960
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..14960
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..14960
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             101..1333
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg3_1"
                     /sec_met_domain="HBT (E-value: 2e-17, bitscore: 56.5,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYKLLVLDVDGTLLNDAKEISKRTLAALLKVQQMGVRIVLASGR
                     PTYGLMPLAKMLELGNYGGFILSYNGCQIINAQNGEILFERRINPEMLPYLEKKARKNG
                     FALFTYHDDTIITDSPENEHIQNEARLNNLQIIKEEEFSAAVDFAPCKCMLVSDDEEAL
                     VGLEDHWKRRLNGALDVFRSEPYFLEVVPCAIDKANSLGALLEVLDMKREEVIAVGDGV
                     CDVTMIQLAGLGVAMGHSQDSVKACADYVTASNEEDGVAVAVEKAIIAEVRAAEIPLDQ
                     LNAQARHALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGILHGGATLALAETVAGLGS
                     MILCQPDEIVVGMQVSGNHISSAHEGDTVRAVGTVVHKGRSSHVWNVDVFTSTNKLVSS
                     IRVVNSVMKKR"
     CDS             1330..2445
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg3_2"
                     /sec_met_domain="Chorismate_bind (E-value: 5.4e-57,
                     bitscore: 186.3, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDEEISNLTTIDAFIQRKQPFAVYRIPGEKVPRLLTQAEGAVRL
                     IYDLKELNGQRGFVIAPFQVSESCPVVLIQPDQWGQPLPMDDDTEEDREIALRLQGQES
                     FLTSSTEEYAACFHTFINALRDRTFDKLVLSRHLTVDKVADFSPLSIFRAACKRYIHSY
                     IYLCYTPQTGIWLGSTPEIILSGEKDEWHTVALAGTQPLQDGRLPQVWDEKNRKEQDYV
                     ASYIRRQLLSLDIHSTENGPYPAYAGALSHLKTDFRFSLKDNKGLGDLLKVLHPTPAVC
                     GLPKEEAYQFILQNEGYDRRYYSGFIGWLDPEGRTDIYVNLRCMHIEDKQLTLYAGGGL
                     LASSELNDEWLETEKKLQTIKRLIAVPPLKS"
     CDS             2465..4132
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg3_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.1e-06, bitscore:
                     21.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYTDKKNILQLVALLEAHGITKVVLCPGSRNTPIVHTLSNHPNFT
                     CYPVTDERSAGYFAIGLALNGGKPAAVCCTSGTALLNLHPAVAEAFYQNVPLVIISADR
                     PAAWIGQMDGQTVPQPGVFQTLVKKSVNLPEIHTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINVPISEPLFQFTVDSLPEVRVITRYQGLNVYDRDYNDLVDRMNKYQKRMIIIGQMN
                     LIYLFEKRYIKLLYKHFAWLTEHIGNQTVPGIPVKNFDAALYAMPEEKVGQMVPELLIT
                     YGGHVVSKRLKKFLRQHPPKEHWHVSPDGEVVDLYGSLTTVIEMDPFEFLEKIASLLDN
                     RTPEYPRVWENYCKIIPEPEFAYSEMSAVGTLLKALPESCALHLANSSVVRYAQLYSIP
                     STIEVCCNRGTNGIEGSLSTAVGYAAASDKLNFIAIGDLSFFYDMNALWNVNVRSNLRV
                     LLLNNGGGEIFHTLPGLDMSGTSHKFIAAVHKTSAKGWAEERGFLYLQAQNDEELAEAM
                     KTFTQPEAMEQPVLLEVFTNKNKDARMLKNYYHQLKQK"
     CDS             4136..4960
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg3_4"
                     /sec_met_domain="ECH_1 (E-value: 1.2e-81, bitscore: 266.7,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYDDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKQYPKFP"
     CDS             5133..6158
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MR_MLE_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg3_5"
                     /sec_met_domain="MR_MLE_C (E-value: 2.2e-27, bitscore:
                     89.1, seeds: 401, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNCKIEIIPRLLHFKQPAGTSRGTYTTRKVWYLHFTSPDFPGRVG
                     IGECAPLPALSCDDLPDYEDILVKVCRKVEREQGRLDMDGLCDYPSILFGLETAIRHFF
                     AGNWALCDTAFSRGEVGIPINGLIWMGDFQNMLSQIEKKMEAGFRCIKLKIGAINFEEE
                     LTLLRHIRTHFSSKEIELRVDANGAFSPVDAMEKLKRLSEFDLHSIEQPICAGQWEEMA
                     RLAAESPLAIALDEELIGCNTLERKQELLSTIRPQYIVIKPSLHGGICGGDEWITEAEK
                     QQIGWWITSALESNIGLNAIAQWCATFSNPLPQGLGTGALFTDNVEVPLEIRKDCLWFC
                     K"
     CDS             6180..7316
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1025:AMP-binding enzyme (Score: 57.1; E-value: 4e-18)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg3_6"
                     /sec_met_domain="AMP-binding (E-value: 8.5e-18, bitscore:
                     57.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFDRKQQRLLLEGKEYTFEDISRLIAGGAEAHSPASWDLYLFLN
                     EWFNDSPVIIVHTSGSTGTPKELIVRKDQMMQSARLTCEFLDLKKGETALLCMNLRYIG
                     AMMVVVRSLVAGLNLIVRPASGHPLADIKEPLRFVAMVPLQVYNTLQVPEEKERLKQTD
                     ILIIGGGAIDEALEAEIKHLPTAVYSTYGMTETLSHIALRRLNGTLASEHYYPFSSVKL
                     SLSVENTLIIDAPLVCDEILQTNDIARIYPDGSFMILGRKDNVINSGGIKIQAEEMEKL
                     LRSFIPVPFVITSVPDQRLGQAVTLLLEGQPELLEGQPDTKEIGNKLQEILEPYYRPKY
                     IWTTDCIPQTENGKVNRAKCRILAKQMLTTFYTHTLTP"
     CDS             complement(7627..8103)
                     /locus_tag="ctg3_7"
                     /transl_table=11
                     /translation="MKTLEFIKLNESGANNVVASLQQLLADFQVYYTNLRGFHWNIKGH
                     DFFVLHSQFEKMYDDTAEKVDEIAERILMLGGTPANKFSDYLKVANISEVDKVSNGEQA
                     LNNILQSISYLIGEERKILSIASQAGDEVTVSMMSDYLKEQEKLVWMLTAYNSK"
     CDS             8242..9168
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) LysR_substrate"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HTH_1"
                     /gene_functions="regulatory (smcogs) SMCOG1012:Bacterial
                     regulatory helix-turn-helix protein, lysR family (Score:
                     61.1; E-value: 2.4e-19)"
                     /gene_kind="regulatory"
                     /locus_tag="ctg3_8"
                     /sec_met_domain="LysR_substrate (E-value: 2.3e-43,
                     bitscore: 141.0, seeds: 361, tool: rule-based-clusters)"
                     /sec_met_domain="HTH_1 (E-value: 5.1e-19, bitscore: 61.1,
                     seeds: 1330, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIQQLEYILAVDQFRHFARAAEYCRVTQPTLSAMIQKLEDELGV
                     KLFDRTVQPVCPTPIGQKVIDQARVILAQAAQVKEIISEDKQSLSGVFRLGVLPTIAPY
                     LLPRFFPQLMEKYPELDIRVTEMKTQDIQQALHAGDLDAGIIASKLEDTFLTEETLFYE
                     QFYAYVSRKEPSFKHDVIRTSDITGEHLWLLDEGHCFRDQLVRFCQMEAVKVSQMAYRL
                     GSMETFMRMVESGKGITFIPELAVAQLTEEQRQLVRPFAIPRPTRQVVLATNRDFIRHS
                     LLCVLKEEIKAAVPKEMLTLQSIQCLL"
     CDS             complement(9581..10303)
                     /locus_tag="ctg3_9"
                     /transl_table=11
                     /translation="MKKFKFIAFIFAIMTTMPILQSCLDDDDNPSDLLVVSTINMISQD
                     SKEFYFTLDDGKKMYPSNAQGWSNKDWVEGQRAFVIFNELEEPVNGYDLNIQVRGINPI
                     LTKDIITMGEDDNDEEKVGDDKINTTYMWINKDNKYLTIEFQYYGTHSEDKKHFLNLVI
                     NDKEESAPTADEDSAEDEYINLEFRHNSEGDYPQSLGEGYVSFKLDKIKDRMEGKKGLR
                     IRVNTLYGGPKTYEVKFP"
     CDS             10480..11028
                     /locus_tag="ctg3_10"
                     /transl_table=11
                     /translation="MEELELSEQCRLGNNQARKELYEQYAGRMLGICLRYTGDRDTAQD
                     LLHDGFLKIFDSFDKFTWRGEGSLRAWMERVMVNTALQYLRKNDVINQSAPLEELPEEY
                     EEPDASDVEAIPQKVLMQFIEELPAGYRTVFNLYTFEDKSHKEIAQELGINEKSSASQL
                     FRAKSVLAKRVKEWITHNG"
     CDS             11035..12333
                     /locus_tag="ctg3_11"
                     /transl_table=11
                     /translation="MEEKELWMNKLKEKLADYSEPVPASGWEQLEKELMPPVEKKIYPY
                     RKWMMAAAAVILLAVVSSVSLYFLGTPAADEIRHIKTPALASTPDALPGVQQPDVQGTS
                     VEPVLRPVAREDRLAKIDKNLTEQKTNAGQSAIENNNEPVTGNENNPVIEEDQMLKGET
                     EQTKNEARQVDSENVGQATQSQDTERPNNRPRRPSGRDKLHIPAEKRASQKGTWSMGLS
                     VGNSGGASTEVGAGSHAYMSRVSMLSVSNGLMEIPNDQTLVFEDGVPYLRQAKQVVDIK
                     HHQPISFGLSVRKGLAKGFSLETGLTYTLLSSDAKLAGEDQQIEQKLHYVGIPLRANWN
                     FFDKKLVTLYVSGGGMVEKCVYGKLGSEKETVKPLQFSVSGAVGAQVNATKRLGIYVEP
                     GVAYYFDDGSDIQTIRKENPFNFNIQAGIRLTY"
     CDS             12546..13142
                     /locus_tag="ctg3_12"
                     /transl_table=11
                     /translation="MVNFTDEKHLLIDLKDGSFQAFERLYNMYSGKLYNFIMRLSSGNQ
                     YMAEEVVQSTFIRIWEVREKVDTNASFISFLCTIAKNLLMNMYQRQTVEYVYNEYLLKS
                     SVDRDSQTEENIDLHFLNDYIDSLAEELPAQRKKIFILSKRQNYTNKEIAEMMGISEST
                     VATQLSLAVKFMREQLMKHYDKVIALLIAFFVNEM"
     CDS             13210..14190
                     /locus_tag="ctg3_13"
                     /transl_table=11
                     /translation="MDKIYYKELIEKYFDGNITDAEIKELSDWIKNDRHLQNWWEEEFS
                     KSDAGINPVLRDKLFARIKEQTQGKEETQEKEKPRTIRMNPWKWAAAIVLPICIAFFTY
                     YLIDSSQTVGAPFIVKADKGDKATIELPDGTNVVLNSASQLSYLNNFGENVRRVQLNGE
                     AYFKVAHDEKHAFIVQVGDLEVKVLGTSFNVSAYEDAKDVTVVLLEGKVGVYAQKMSHI
                     MKPGDKIEYNKATHKITATQVHPSDYIEWTKGNIYFEKESLENIMKTLSRIYDVEIRFD
                     SNKLPNEYFTGTIPVGGIQNALNILMLTSPFYYEMDGSVIVLKEK"
ORIGIN
        1 gaagcagagt ttgagggttg gcctcggttt ttattgtgcc ggtgtagaaa aactcgattt
       61 ttctatataa ttttgtaatc taaatcaaaa tcgtgtaatc atgaagtata aattattggt
      121 tcttgatgta gacggaacac tcctcaatga tgcgaaagaa attagtaagc gtacattggc
      181 tgccctgttg aaggttcagc aaatgggagt acgtattgtg ctggcatccg gcagaccgac
      241 ttatggcctg atgccgttgg ccaagatgct tgaacttgga aactatggag gttttatcct
      301 ttcctataat ggctgtcaga ttatcaatgc acaaaacggg gaaattctgt ttgaacgtcg
      361 gattaatccc gaaatgttgc cgtatctgga aaagaaagcc cgtaaaaacg gttttgcctt
      421 attcacttat catgatgata cgattataac ggattctcct gaaaatgaac atatccaaaa
      481 tgaagcccgg ttgaataacc tgcaaataat taaagaagaa gaattttctg ctgccgttga
      541 ttttgctcct tgtaagtgta tgctggtgag cgatgacgaa gaagcactgg ttggtttgga
      601 agatcactgg aagagacgac tgaacggagc gttggacgta ttccgttcgg agccatattt
      661 tctggaagta gttccctgcg ccatcgacaa agccaactct ttgggcgctt tgctggaggt
      721 gctggatatg aagcgtgagg aagtgattgc tgtgggcgac ggtgtgtgtg acgtaacaat
      781 gattcaattg gccgggctgg gcgtagctat gggacattca caagactcgg tgaaagcctg
      841 tgccgactat gtgaccgctt cgaatgagga agacggagtg gccgttgcag tggagaaggc
      901 tattatagct gaagtgcgtg cagccgaaat tccccttgac caactgaatg cgcaggcacg
      961 ccacgcatta atgggaaact tgggaattca atatacatac gccgacgaag accgtgtgga
     1021 agcaacgatg ccggtagacc atcgtacccg tcagcctttc ggcattttgc atgggggagc
     1081 tactcttgca ctggcagaaa cagttgccgg actcggttcg atgattcttt gccagccgga
     1141 cgagattgta gtaggaatgc aggtcagcgg aaaccatata tcttctgcac atgaaggaga
     1201 tacagtgcgt gcggtaggaa cagttgtaca taagggacgt tcctcccacg tatggaatgt
     1261 agacgtgttt acttcaacaa acaaactggt gtcttccata cgggtagtca acagtgtgat
     1321 gaaaaagaga tgaccgacga agaaataagt aacttgacaa ctattgatgc atttattcag
     1381 cggaagcagc cgtttgctgt ttaccgtatt cccggagaga aggttccccg tctgttgacg
     1441 caggcggaag gagcggtccg tttgatatac gatttgaaag aactgaacgg acagagagga
     1501 tttgtaattg ctccgtttca ggtgagtgag tcatgtccgg ttgtgttgat ccaacccgat
     1561 cagtgggggc agcctttacc gatggatgat gatacggaag aagaccggga gatagccttg
     1621 cgcctgcaag gacaagaatc atttctgact tcttctacgg aagaatatgc ggcatgcttt
     1681 cataccttta taaatgcgtt acgtgacagg acgtttgata aactagtgct ttcccgtcat
     1741 cttacagttg ataaagttgc ggatttttct cctttgtcca tctttcgggc ggcttgtaaa
     1801 cgttatattc attcgtatat ttacttatgt tatactccgc agacgggcat ctggctggga
     1861 agtactcccg aaatcatttt atcaggtgag aaagacgaat ggcatacggt tgcactagcc
     1921 ggaacacagc ctttgcaaga tggcagattg ccgcaagtat gggatgaaaa gaaccggaaa
     1981 gaacaagatt atgttgcttc ttatatccgt cggcagcttc tttcactgga tattcattct
     2041 acggaaaacg ggccatatcc tgcttatgcc ggtgctttgt cacatttaaa aactgatttc
     2101 cgtttttctt tgaaagataa taaaggtttg ggagaccttc tgaaagtttt gcatccgaca
     2161 cctgccgtat gcggcttgcc gaaggaagaa gcctatcagt ttattttgca gaatgaaggt
     2221 tacgaccgcc gctattactc cggctttatc ggatggctcg atccggaagg acgaacagac
     2281 atctatgtaa acttacgctg tatgcatatc gaagataaac aacttactct ttatgccggc
     2341 ggtggattat tggcctcttc ggaattgaat gacgaatggc tggaaacgga gaagaagttg
     2401 caaactatca aacggctgat tgcagtgcct cctttaaaat cataaactac taataattat
     2461 catcatgtat acagacaaga agaacatact ccagttggtt gcgttgctcg aggcgcatgg
     2521 gattactaag gttgtgttgt gtccgggtag tcggaatacg cccatagtac atactttgtc
     2581 taaccatccg aattttacct gctatccggt gacggatgaa cggagtgccg gctattttgc
     2641 catcggtctt gcgttgaatg gtggtaagcc cgcagctgtc tgttgcactt ccggtactgc
     2701 attattaaac cttcatccgg ctgtggcaga agctttttat caaaatgttc ctctggtcat
     2761 tatttctgcg gatcgtcccg ctgcctggat cgggcagatg gatggacaga cagtaccaca
     2821 accaggcgta tttcagacat tagttaagaa gtcggtaaac cttccggaga ttcatacgga
     2881 agaagatgaa tggtattgca atcgtctggt gaatgaagct ttgctggaaa cgaaccatca
     2941 tggaaaagga ccggtacata tcaatgtccc tatttctgaa ccgttgttcc agtttacggt
     3001 tgattcgctt cctgaagtgc gtgtcattac ccgttatcag gggttgaacg tctacgatcg
     3061 tgattataac gatttggtcg atcggatgaa taaataccag aagcgaatga ttattatcgg
     3121 tcagatgaat cttatctacc tgtttgaaaa aagatatatc aagttattat ataaacattt
     3181 tgcctggttg acggaacata tcggcaacca gacagttccc ggtattccgg taaagaactt
     3241 tgatgcggcg ctttatgcca tgcccgaaga gaaggtcggg cagatggttc cggagttgct
     3301 gattacttat ggaggacatg tcgtttccaa acggctgaag aagtttctcc gtcagcatcc
     3361 gcctaaagaa cattggcacg tatcgccgga tggtgaggtg gtcgatttgt acggctcatt
     3421 aactacggtg atcgaaatgg acccgttcga gtttctggag aagatagcca gtttgttgga
     3481 caatcggact cctgaatatc cccgtgtgtg ggaaaactat tgcaagatca ttcccgaacc
     3541 ggagtttgct tattcggaaa tgtctgccgt cggtacgtta ttgaaagcct tgccggagtc
     3601 atgtgcgttg catctggcta acagttcggt ggtgcgctat gcacaattat actctattcc
     3661 ttctacgatt gaagtttgtt gcaaccgggg gacaaacggc attgaaggct cactgtctac
     3721 ggctgtcggt tatgcggcag cttccgacaa actgaacttt atagctatcg gagatttgag
     3781 cttcttttat gatatgaatg cgttgtggaa cgtcaatgtt cgctccaatc tgcgtgttct
     3841 tctgttaaat aatggaggag gagagatttt ccataccttg ccgggattgg atatgtcggg
     3901 tacctcacat aaatttattg cggcagtcca taaaacgtcc gccaaaggtt gggcagaaga
     3961 gcgtgggttt ctctatctgc aagcgcaaaa tgacgaagaa ctggctgaag cgatgaaaac
     4021 tttcactcaa ccggaagcta tggagcagcc tgtgctcttg gaggtgttta ccaacaaaaa
     4081 caaggacgcg cgcatgttga agaattatta tcatcaatta aaacaaaaat agattatgtc
     4141 aacacaaaga gagtggacaa ccatcagaga atacgacgat attctttttg attattataa
     4201 tggaattgcc cgtatcacga tcaaccgtga acgctatcgg aatgcgttta ctccgacaac
     4261 tactgctgaa atgagtgacg cattgcgcat ctgccgtgaa gaagcggata ttgatgtgat
     4321 tgtgattacc ggagccggtg ataaggcgtt ttgttcgggt ggcgaccaga atgtgaaagg
     4381 acgtggcgga tatatcggaa aagacggtgt gcctcgtctt agcgtattgg atgtgcaaaa
     4441 gcagattcgc agcatcccga aaccggtgat tgctgccgtg aacggttttg ctattggtgg
     4501 cggacatgtg cttcatgtgg tatgtgattt gtccattgcg tcggaaaatg ctattttcgg
     4561 tcagacgggt cctcgtgtag gtagctttga tgccggattc ggtgcgtctt atctggcacg
     4621 tgtggtaggt cagaagaaag cacgcgaaat ctggttcctc tgtcggaagt ataatgcaca
     4681 agaagcattg gacatgggat tagtgaataa agtagttcct ttggagcagt tggaggacga
     4741 atatgtgcag tgggcagaag aaatgatgtt gcttagtccg ttggctttgc gcatgattaa
     4801 agcaggattg aatgccgaac tggatggtca ggcaggtatt caggaattgg caggtgacgc
     4861 aacgttgctc tattacctga ctgatgaagc acaggaagga aagaatgcgt ttctggagaa
     4921 acgtaaaccg aacttcaaac aatatccgaa attcccttaa aatcggcttt gccacagagt
     4981 accacagagt ttgcttttcc tgcgtttaag cccgattttc tgcaaagcaa gactgtggag
     5041 gagagaatgg taggagaact gcaagagata taacttatta aaacttcgtg aggctctgtg
     5101 ttattctgtg gtgaattgaa aaattattca cgatgaattg taaaatagaa attattcccc
     5161 gattgctcca tttcaagcaa cctgccggga cttccagagg aacatatact acgcgtaaag
     5221 tttggtatct tcattttacc tctcctgact tccctggcag agtgggaata ggggagtgtg
     5281 cacctttgcc cgctttaagt tgtgacgatc ttcctgatta cgaagatata ttagtgaaag
     5341 tctgccggaa agtagagagg gaacaaggca gattggatat ggatggcttg tgtgactatc
     5401 cttcaatttt atttggtctg gaaacggcca tccggcattt ctttgcagga aactgggctt
     5461 tatgtgatac ggctttctca cgtggagaag ttggtattcc gattaacggc cttatttgga
     5521 tgggagattt tcagaacatg ttatcccaga ttgagaaaaa gatggaagcc ggatttcgtt
     5581 gtatcaagtt aaagataggt gccatcaatt tcgaagaaga actgacattg ttgcgccata
     5641 ttcgtacgca tttctcttct aaagaaatag agttgcgtgt agatgcaaat ggtgctttct
     5701 cacccgtgga cgcaatggaa aaactgaaac gtctgtccga gtttgattta cattctatcg
     5761 aacagccaat ctgtgccgga caatgggagg aaatggctcg gcttgctgcc gaatctccat
     5821 tagcgattgc tttggatgaa gaactgatag gatgcaatac gctggaaagg aaacaggagc
     5881 tgctttcaac aattcgtccg caatatatcg ttattaaacc ttctcttcat ggaggaatat
     5941 gcggaggaga cgaatggatt accgaagctg aaaaacaaca gatcggctgg tggataactt
     6001 ccgctctgga atcaaacatt gggctaaacg ccattgccca gtggtgtgca acattcagca
     6061 atccgttgcc acagggactg ggaacagggg cgctctttac agacaacgtg gaagtgcctc
     6121 ttgaaataag aaaagattgt ttatggtttt gtaaatgata gtatttaaag gatattccga
     6181 tgatattcga tcgaaaacaa caacgcttgt tactggaagg aaaagaatat acttttgagg
     6241 atataagccg attaatagca ggaggagcgg aggctcattc tcctgctagt tgggatttat
     6301 atcttttcct gaacgaatgg tttaacgatt ctcctgtcat cattgttcat acatcaggct
     6361 ctacgggtac acctaaagag ttgattgtac gtaaagacca gatgatgcaa agtgcgcgtc
     6421 tgacttgtga gtttctggat ttgaagaaag gagagacggc attactttgt atgaatcttc
     6481 gttatattgg tgcaatgatg gtggtcgtgc gttcgttggt tgcaggcttg aatttgattg
     6541 tacgtcctgc ttccggtcat cctttagcgg atataaaaga gcctttgaga tttgtggcaa
     6601 tggttcccct gcaagtttat aatacgttgc aagttcccga agagaaagaa cgattgaaac
     6661 agacggatat tttgattata ggtggtggag ctattgatga ggctttagag gcggagatca
     6721 aacatcttcc gactgccgtc tattctacct atggcatgac tgaaacttta tctcatattg
     6781 ctcttcgtcg gctgaacgga acattggctt cggaacatta ttatccgttt tcttcagtaa
     6841 aactgtcttt atctgtagag aatacgttaa ttattgatgc ccctttggta tgtgatgaaa
     6901 tattgcaaac caatgacatt gcacgcatct atcccgatgg aagttttatg attttgggtc
     6961 ggaaagacaa tgtgataaat agtggaggaa ttaagattca ggcggaggaa atggagaaat
     7021 tgcttcgttc ttttattcct gtgccatttg ttattacttc agttccggac cagcgtttag
     7081 ggcaggctgt aaccttacta ttggaaggtc agccagaatt attggaaggt cagccggaca
     7141 ctaaagaaat cggaaacaaa ctacaggaaa tattggaacc ttattatcgt cccaagtaca
     7201 tatggacgac agattgtatt ccgcaaacag aaaacggcaa ggttaatcgt gcgaagtgtc
     7261 gtattttagc caaacaaatg ttgacgacgt tttacactca cactctcaca ccttgattga
     7321 aatcctttat tcatcaacgt ttcaggggcc tctaattgtc ttaccctagt tttagttgag
     7381 acttattaga gtcaacaaaa ttcagggtaa gacattttat ttcagtagaa taagtttcat
     7441 cttttgaaag ttgttgctta cttttataaa tcttgataaa taaagaacaa aactaatttt
     7501 tttaataata tcagcgaatg aacgtattta gatagtacta gtacactgga aataataaag
     7561 agagaatatt ccttttacgg aaatatcctc tctttctcac ttaaaaacct aaacctttta
     7621 tcttaattat ttgctattat aagccgtcaa catccaaact agtttttcct gttctttcag
     7681 ataatcgctc atcatcgaaa cggttacttc atctccggct tgagaagcga tagacaagat
     7741 tttacgttct tcaccaatca gataactgat actctgaagg atattgttca gtgcctgttc
     7801 gccgttgctc accttatcta cttcactgat atttgctact ttcaaataat cgctgaattt
     7861 attggcagga gtaccgccca acatcaagat acgttctgca atttcgtcta ccttttcggc
     7921 tgtgtcatca tacatctttt cgaattggct gtggagaaca aagaaatcat gtcctttgat
     7981 attccagtgg aaaccgcgga gattggtgta atacacttgg aagtctgcta atagttgttg
     8041 caaagatgct actacgttgt ttgctcccga ttcattcagt ttgataaatt ctaaagtttt
     8101 catattcttt ttatttttaa tgatttatac ttgttttctt ttttttctgt actgcaaaga
     8161 taaggcggaa aatgcttgtt gtcaaactga aaatttctat ctttgcatag atgaaatcta
     8221 taacttaata tgatttgata gatgacgata caacaattag aatatatact agccgtagac
     8281 cagttcaggc atttcgcacg ggcagccgaa tattgccgtg tgacgcaacc tactttgagt
     8341 gccatgattc agaagctgga agatgaattg ggagtaaagt tattcgatag gacagtgcaa
     8401 cccgtctgcc cgacacccat cgggcagaaa gtgatagacc aggctcgtgt gattctggcg
     8461 caggcagctc aagtaaaaga aatcatcagt gaagataagc aatcattatc aggtgtattc
     8521 cgtttgggag tattacccac tatcgctcct tatttgctac cccgcttttt ccctcaactg
     8581 atggagaaat atcccgaact ggatattcgt gtcacggaaa tgaaaaccca ggatattcag
     8641 caggctctgc atgccggcga tttggatgca gggattattg ccagtaaatt agaagacaca
     8701 ttcttgacgg aagaaactct cttctatgag caattttatg cttatgtatc acggaaagaa
     8761 ccctcgttca aacatgacgt tatccgcact tccgatataa ccggcgaaca tctttggctt
     8821 ttggacgaag gacattgctt tcgtgaccaa cttgtccgtt tctgtcagat ggaagcggtg
     8881 aaggttagtc agatggctta tcgtttggga agcatggaaa cctttatgag aatggtagaa
     8941 agcggaaaag gaatcacttt tattcccgaa ctggcagtgg ctcaattgac ggaagaacag
     9001 cgacaattag tacgtccgtt tgctatcccc cgaccgacgc ggcaagtggt gttggcgaca
     9061 aatagagatt ttattcgtca tagcctgcta tgtgttctga aagaggaaat aaaagctgca
     9121 gtccccaaag aaatgcttac tttgcagtcc atacaatgtt tgctataata aataggagat
     9181 tgagaggatt ccggagaaat tcataggagt gaagtcatag ctcgatcctt gcttgataaa
     9241 tgtgtttacg atgtgctcca tgtggtagag gcaaatatga tgtaaaaaag tcctttgttc
     9301 attttattgg cttgtctttg ctttcatttt tgtattagtg gtgctcggct ctaccgattc
     9361 gaagagggga gcgggcaatc tggcaggact tgccatcggt ttgactcttg tattggtaca
     9421 tattgtatgt attcccatca cagggacttc cctttataag aataacttct tatataaaga
     9481 taaaagggcg gcagtttcac aactaccgcc ctcttttgcg taattaatat attatattaa
     9541 aaaagagtgt gttttgcacg tttacattag atttataatc ttagggaaac tttacttcat
     9601 aagttttcgg tccgccatat aaagtgttga cacggatcct taatcctttt ttcccttcca
     9661 tccggtcttt aatcttatcc agtttgaaag atacatatcc ttcgcccagg ctttgaggat
     9721 aatcaccttc actgttatgg cggaattcca gattgatata ttcgtcctcc gcactgtctt
     9781 cgtcagcagt gggagcagac tcttctttgt cgttgattac gagattcagg aaatgttttt
     9841 tatcttcact atgcgttcca taatattgga attcaatcgt cagatattta ttatccttgt
     9901 tgatccacat ataagttgtg ttgatcttgt catctccgac tttttcttcg tcgttgtcat
     9961 cttcacccat cgtgatgatg tctttagtta agattggatt gatacctctc acctgaatat
    10021 taaggtcgta tccattcaca ggctcttcca gctcgttgaa tatcacaaaa gctcgttgtc
    10081 cttccaccca atctttatta ctccatcctt gcgcattgct gggatacatc tttttcccgt
    10141 catccaaagt gaaataaaat tccttgctat cctggctaat catattgatg gtactgacaa
    10201 ccagaagatc ggacgggttg tcatcgtcgt ccaaacatga ctggagaatc ggcattgtag
    10261 tcataatagc aaagataaag gcaatgaatt taaatttctt catatactta tttaagtttt
    10321 agattatata aaatggttcc tttgtattgt gaaaatgcag tggaatagaa aatattgcat
    10381 ttgaatctgt taaagaaaca aaagtgataa aagagttgtt ctctctatgc agtatttcta
    10441 tctttgctgc atttataagt taaacacgtg tataagagaa tggaagaatt agagttgtcg
    10501 gaacaatgca ggctaggcaa taaccaagct cgcaaagaac tgtatgagca gtatgcggga
    10561 cgtatgcttg gcatctgtct tcgctatacc ggcgaccgtg atacagccca ggatctgctt
    10621 cacgacggtt ttttaaagat attcgattcc ttcgacaagt tcacctggag gggtgaaggc
    10681 tccctgcggg cctggatgga aagggtgatg gtcaacacag ctttgcaata tctccggaaa
    10741 aatgatgtaa tcaatcagtc ggcaccatta gaagagttgc ctgaagaata tgaagaaccg
    10801 gatgcttctg atgtagaagc tattccacag aaagtgttga tgcagtttat agaagaacta
    10861 cctgccggat atcgtacagt gtttaacctt tatacattcg aagacaaatc gcataaagaa
    10921 atcgcacagg aattaggtat taatgaaaaa tcttcggctt cgcaactatt tcgtgcgaag
    10981 agtgtattgg caaaaagagt aaaagaatgg ataacgcata atggatagat agagatggaa
    11041 gagaaagaat tgtggatgaa taagttgaag gagaagctcg cggattattc cgagccggtg
    11101 cctgcttccg gttgggaaca actggagaaa gaacttatgc ctcctgtgga gaagaaaata
    11161 tatccttatc gaaaatggat gatggctgct gcagctgtta tattgttagc tgtcgtttcg
    11221 tcggtaagtc tatattttct ggggacaccg gctgcggatg aaatacgtca tataaagact
    11281 ccggcattgg catctacacc cgatgcgttg ccgggtgtgc aacaaccaga tgtgcagggc
    11341 acttccgtag aacccgtctt gcgaccagtg gctcgtgaag accgtttggc gaaaattgat
    11401 aagaatctta cagaacagaa aacaaatgca ggtcaatctg caatagaaaa taacaacgaa
    11461 cctgtaacag ggaatgaaaa taatcctgtg attgaggaag accagatgct caaaggtgag
    11521 acggaacaga cgaaaaatga agcaaggcag gttgacagtg aaaatgtggg tcaggctaca
    11581 cagtcacagg acaccgaacg gccgaacaac aggccgcgtc gcccttccgg cagagacaag
    11641 ctgcatattc cggcagaaaa aagagcatct caaaaaggaa catggtccat gggactctcg
    11701 gtcggaaatt cgggcggagc atccacagaa gtcggtgccg gctctcatgc ttatatgtct
    11761 cgtgtgagta tgctttctgt ttccaacggt ctgatggaga tacctaacga tcagacattg
    11821 gtttttgaag atggagtgcc ttatctgcgt caggcaaaac aagtggtcga cattaagcac
    11881 catcaaccta tttctttcgg attgtctgtc cgtaaaggac tggccaaagg attctctctc
    11941 gaaacgggat tgacttacac attgctttct tcggatgcga aactggcggg tgaagatcag
    12001 cagatagaac aaaaacttca ttatgtcggt attccgctgc gtgctaactg gaatttcttt
    12061 gataaaaaac tcgtcactct ctacgtctcc ggtggaggta tggtggagaa atgtgtttat
    12121 ggaaagctcg gttccgagaa agaaacggtg aaacctttgc aattctcggt atctggtgcg
    12181 gtaggtgcac aggtgaacgc aacgaaacgt ctgggtatct atgtagaacc cggtgttgct
    12241 tattattttg atgacggttc ggatatacag accatacgta aagagaatcc gtttaacttc
    12301 aatatacagg ctggtatccg tttgacttat taatgcgaat taccatactg aacatagtga
    12361 cggtatccgt tcaagatagc gtcactatcc tgattagata gcgtcactat cttgaccgga
    12421 tggcgtcact atcctgatat tagtatcttt ttcctcataa aaatcggata attgagaaaa
    12481 tattaataat attgtcacct gttaatggtc tatggttgca tacctttgtt gcaaaattct
    12541 aagctatggt gaattttaca gatgaaaaac atttattaat cgatttaaaa gacggttctt
    12601 ttcaagcatt tgaaagattg tataatatgt acagcggtaa actgtataat ttcattatgc
    12661 ggctttcttc cggcaatcaa tatatggctg aagaagtggt gcaatccact tttatccgta
    12721 tatgggaagt acgtgagaaa gtcgatacga acgcttcatt tatttctttt ctttgtacga
    12781 tagccaaaaa tttactgatg aacatgtacc agcgtcagac cgttgaatat gtttacaatg
    12841 aatatctgct gaaaagcagt gtggatcgtg attcgcaaac agaagagaat attgatcttc
    12901 atttcttgaa cgactatatc gactcattag cggaagaatt gccggcacag cgaaagaaaa
    12961 tctttatctt aagtaagcgt cagaactata cgaataagga aattgctgaa atgatgggta
    13021 tttctgaaag tacagtcgcc acacaattat ctttggcggt gaaatttatg cgtgagcagt
    13081 tgatgaagca ttacgataag gtaattgcgc ttctgatcgc cttttttgtt aatgaaatgt
    13141 aattgtaata catgtttttg gatagtagat aggatgaaaa tgtatacttg taaataagca
    13201 ggaaagaaga tggataagat atattataaa gaactgattg aaaagtattt cgatggaaat
    13261 ataacggatg cggagattaa agaactttcc gattggataa agaacgaccg ccatttacag
    13321 aactggtggg aagaagaatt ttccaaatcg gatgccggta ttaatccggt actgcgcgac
    13381 aaactatttg cccgaatcaa agaacagacg cagggaaaag aagagacaca ggaaaaagag
    13441 aaaccaagaa ctattcgcat gaatccttgg aaatgggctg ccgccatcgt tttacccatt
    13501 tgtattgcct tcttcaccta ctatcttata gattcttccc aaacagtggg agcacccttt
    13561 atagtaaaag ctgataaagg agataaagca accatcgaat tgccggacgg aacgaatgtc
    13621 gttctcaatt ctgcttcgca attaagctat ctgaataact tcggagaaaa tgtgcgtcgt
    13681 gtgcaactga atggtgaagc ctatttcaaa gtagctcatg atgaaaaaca tgcctttata
    13741 gtgcaggtcg gtgatttgga agtgaaagta ctcggtactt cttttaatgt atctgcctat
    13801 gaagatgcca aagacgtgac agtcgttctt ttggaaggaa aagtaggggt ctatgcgcag
    13861 aaaatgtcgc atatcatgaa gcccggcgac aaaatagaat ataataaagc cactcataag
    13921 ataacagcca ctcaagtaca cccgagtgac tacattgaat ggacgaaagg aaatatctat
    13981 ttcgagaaag aatctttgga gaatataatg aaaacccttt cacgcatcta tgacgtagag
    14041 attcgtttcg actccaataa actacctaat gaatatttca ccggaaccat tccggttggt
    14101 ggcatacaga atgcattgaa tatcctgatg cttacttcac ctttctatta tgaaatggac
    14161 ggttcggtga ttgttttgaa agagaaataa gtggaacttt catagagtat ctttgtttat
    14221 tcatcgaata acgtctatat ttgtggtaca ctaatttgaa gatgcaatga attcattgga
    14281 acgtaaattg ccgataggga tacagacatt tgaaaagatg cgtgcagagg ggtgtttgta
    14341 tgtggataaa acagccatta tatatcagat agcagctact aaagtgcctt attttcttag
    14401 tcgtccccgt cgttttggca aaagtctgct aatttccact tttgaagcct attttcaggg
    14461 tcgtaaagac ctttttgaag gcttggcaat tgaaaagtta gagacgaagt gggagcaata
    14521 tccggttctg catcttgatt tgaatgcgaa gaaatatgag acggcggcag atttggttgc
    14581 catgctaaac cagtatttgg agaaatggga agccgtatat ggtgatgaga agaaagaccg
    14641 tagtcctgaa gaacgtttta gttatatcat tgagcaagcc tatctgaaaa caggaaaagg
    14701 agttgtggta ttggttgatg agtatgataa acctttgctg caagcccttt tagatgagaa
    14761 cctgttggat gaataccgtc gtattctgaa agccttttat ggtgtactga aaagttccga
    14821 ccgctatctt cggtttatat tcttgacagg agtcacaaaa tttgcccaag taagtgtgtt
    14881 cagtgatttg aatcagctga atgatataag tatgaaaatt ccatacgcca atatttgtgg
    14941 tattactaaa aaggagttag
//
