GCA_020257125.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaBGPDPNMD_11 (original name was: gnl|Prokka|BGPDPNMD_11)

Region	Type	From	To	Most similar known cluster		Similarity
Region 11.1	NRPS-like	90,470	132,899

gnlProkkaBGPDPNMD_16 (original name was: gnl|Prokka|BGPDPNMD_16)

Region	Type	From	To	Most similar known cluster		Similarity
Region 16.1	RRE-containing	170,712	191,065	polysaccharide C	Saccharide	20%
Region 16.2	phosphonate	487,865	516,072	polysaccharide A	Saccharide	40%

gnlProkkaBGPDPNMD_17 (original name was: gnl|Prokka|BGPDPNMD_17)

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	phosphonate	496,891	520,733	polysaccharide B	Saccharide	30%

gnlProkkaBGPDPNMD_20 (original name was: gnl|Prokka|BGPDPNMD_20)

Region	Type	From	To	Most similar known cluster		Similarity
Region 20.1	phosphonate	43,281	56,840	polysaccharide B	Saccharide	13%

Region	Type	From	To	Most similar known cluster	Similarity
Region 11.1	NRPS-like	90,470	132,899
Region 16.1	RRE-containing	170,712	191,065	polysaccharide C	Saccharide	20%
Region 16.2	phosphonate	487,865	516,072	polysaccharide A	Saccharide	40%
Region 17.1	phosphonate	496,891	520,733	polysaccharide B	Saccharide	30%
Region 20.1	phosphonate	43,281	56,840	polysaccharide B	Saccharide	13%

No secondary metabolite regions were found in these records:: gnlProkkaBGPDPNMD_1 (original name was: gnl|Prokka|BGPDPNMD_1); gnlProkkaBGPDPNMD_2 (original name was: gnl|Prokka|BGPDPNMD_2); gnlProkkaBGPDPNMD_3 (original name was: gnl|Prokka|BGPDPNMD_3); gnlProkkaBGPDPNMD_4 (original name was: gnl|Prokka|BGPDPNMD_4); gnlProkkaBGPDPNMD_5 (original name was: gnl|Prokka|BGPDPNMD_5); gnlProkkaBGPDPNMD_6 (original name was: gnl|Prokka|BGPDPNMD_6); gnlProkkaBGPDPNMD_7 (original name was: gnl|Prokka|BGPDPNMD_7); gnlProkkaBGPDPNMD_8 (original name was: gnl|Prokka|BGPDPNMD_8); gnlProkkaBGPDPNMD_9 (original name was: gnl|Prokka|BGPDPNMD_9); gnlProkkaBGPDPNMD_10 (original name was: gnl|Prokka|BGPDPNMD_10); gnlProkkaBGPDPNMD_12 (original name was: gnl|Prokka|BGPDPNMD_12); gnlProkkaBGPDPNMD_13 (original name was: gnl|Prokka|BGPDPNMD_13); gnlProkkaBGPDPNMD_14 (original name was: gnl|Prokka|BGPDPNMD_14); gnlProkkaBGPDPNMD_15 (original name was: gnl|Prokka|BGPDPNMD_15); gnlProkkaBGPDPNMD_18 (original name was: gnl|Prokka|BGPDPNMD_18); gnlProkkaBGPDPNMD_19 (original name was: gnl|Prokka|BGPDPNMD_19); gnlProkkaBGPDPNMD_21 (original name was: gnl|Prokka|BGPDPNMD_21); gnlProkkaBGPDPNMD_22 (original name was: gnl|Prokka|BGPDPNMD_22)

Compact view

gnlProkkaBGPDPNMD_11 - Region 1 - NRPS-like

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Location: 90,470 - 132,899 nt. (total: 42,430 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 90469 - 132899:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg11_117

AMP-binding (12..401): X

nrpys: Leu, Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALFLGVVLK
Nearest Stachelhaus code(s):: Leu or Ile DAIYLGVVLK (59% 8Å match); aIle DALVFGVVLK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

gnlProkkaBGPDPNMD_16 - Region 1 - RRE-containing

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Location: 170,712 - 191,065 nt. (total: 20,354 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

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core biosynthetic genes

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaBGPDPNMD_16 - Region 2 - phosphonate

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Location: 487,865 - 516,072 nt. (total: 28,208 nt) Show pHMM detection rules used

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

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core biosynthetic genes

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaBGPDPNMD_17 - Region 1 - phosphonate

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Location: 496,891 - 520,733 nt. (total: 23,843 nt) Show pHMM detection rules used

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

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core biosynthetic genes

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KnownClusterBlast

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaBGPDPNMD_20 - Region 1 - phosphonate

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Location: 43,281 - 56,840 nt. (total: 13,560 nt) Show pHMM detection rules used

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1