LOCUS       c00108_gnlPro..         3670 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KCEKGKEI_376.
ACCESSION   c00108_gnlPro..
VERSION     c00108_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 05:53:37
            Original ID  :: gnl|Prokka|KCEKGKEI_376
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3670
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3670
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[146:3172]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      147..3172
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3670
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3670
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(147..875)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 77.8;
                     E-value: 3.2e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg108_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.7e-29, bitscore:
                     89.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGE
                     STETTIDEIVRMAVSQKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQSIHICIDTN
                     GSIWNEEVKELLQWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAGWLEKNGKPFWLRY
                     VLVPGYSSFEEDIRALGEHFKNYQMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTL
                     KQLETAKTLFGEYFNTVYLN"
     CDS             complement(944..3172)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg108_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-48, bitscore:
                     151.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMNKVFKDGLWSKEINVSDFVHTNITPYEGDASFLAGPTERTKK
                     VWNECLKALEEERANNGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKP
                     YGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLHNITSPMTDARIRLREEVAEQIKALKEIK
                     ILGEYYGLDLGRPATNAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDH
                     GKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLL
                     NFEEVWSNYKKVLMQIARVYNDAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENAYPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDAQDGISNTFSIVPKSLGVDQETRIENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFIKLSREHQLEVISRSFHERM"
ORIGIN
        1 agttctgctg tctgatacaa gaggataaat tgaattgata gaactcacca taataattta
       61 tgttttagat gcgaaattag ataattttat tgaaatgatg aaatatttcc attgatttat
      121 agcaacatct tgtcttcagt atatttttaa tttaaatata cggtattgaa atattcacca
      181 aataacgttt tggctgtttc caattgtttt agagtatttt cttttattcc gtttagtttg
      241 tattcttttc ccatggcttc atatttgtgt acgcctaatg tatgataagg tagtatttct
      301 actctttgaa tcatttgata attcttgaag tgctctccta atgcccgtat atcttcctca
      361 aaggaactat agcctggaac taatacatag cgcaaccaaa aaggctttcc gtttttttca
      421 agccatccgg cggtacgtat ggtttgttcg ttgctgcgtt ctgtcagttg ccgatggcgc
      481 acattattaa attccttgat gtctaataat acgaggtctg tccattgtag cagttccttt
      541 acttcttcat tccatatact gccgttggta tctatacaga tgtggatgct ttgttctttt
      601 aatcgctgga ataacggaat cagtgctttt gcttgcaatg taggctcgcc gccgctgaaa
      661 gtcacacctc cttttttacc gaaaaaagct ttttggctga cggccatacg cacaatttcg
      721 tctattgttg tttccgtact ttcacctttg gtgtcaattg tgtcggggtt ggcacaatag
      781 aggcatcgga aattgcatcc ttgtaaaaat acgactaagc gtaaaccggg accgtcaaac
      841 gtccccattg actcgtaaga atggactctg atcataatgg tttgtcttta aactggtaag
      901 ggcaggttta aaacccgccc ctacgatgaa tgtatatgtg atgttacatt ctttcgtgga
      961 aagaacggct gattacttcc agctgatgtt cacggctcaa tttgatgaag tttacggcat
     1021 aacctgatac gcggatggtt agttgcgggt atttttcagg atgttccatt gcatcttcca
     1081 acatttcacg gttcagcaca tttacattca gatgatgtgc tcctttggtg aaataaccgt
     1141 ccatcatggt taccaggttc tctatacggg tttcctggtc tacccctagt gattttggta
     1201 cgatagagaa cgtgttactg atgccgtcct gggcatcacg atagcgtagt ttggctacag
     1261 aactcaatga ggcgatggca ccgttcttgt cacgtccgtg catcgggttg gcacccggag
     1321 cgaaagccac tcctttggca cgaccatcag gagtggcacc tgttttcttg ccgtacatta
     1381 cattggaggt gatagtcagc agtgacaggg tagggcgggc atttttgtat accggatgtt
     1441 tctttaattc ttcactgaag aaataaacca aatctacccc taagtggtct acgcggtcat
     1501 catcattacc gaagcaagga tatgcatttt caatatcaaa gccttcggtc aaacctatat
     1561 cattacgttt ggcacgtact ttgccgtatt tgatggcaga caatgagtca atggcaatgg
     1621 acagaccggc tacgccatat gccagattga tacgtggatc ggtatcaatg aatgccattt
     1681 gagccttttc ataataatat ttatcgtgca tatagtgaat gatattcatc gcatcattat
     1741 acacacgtgc aatttgcatt aatactttct tataattaga ccatacttct tcaaagttca
     1801 gcaagtcccc tgtcaataca ggaatatctt tcaccattac agttcctgta ttctcacaac
     1861 gtccgccatt gatagccagc aatagggctt tagccagatt gcaacgtgct ccgaagaact
     1921 ggatttgttt gccgatagcc tgataagaaa cgcagcaggc gataccataa tcatcacagt
     1981 tacgcacttc gcgcatcagc gtgtcgtttt cgtattgtat agaagaagtg tcgatagaaa
     2041 cttgtgcaca gaattctttg aaaccttccg gcaattccgg actccacagt acagtcatat
     2101 taggttcggg tgacggaccc agattgtata gtgtctgcaa gaaacggaag gaagtttttg
     2161 taactttggt acgtccgtcg ttgaagcgtc cgccgataga ctcggttacc caggtgggat
     2221 cacctgcaaa gatatcgttg tatgattgca tacgcagatg acggaccata cgcaacttga
     2281 taatgaactg gtctaccagt tcctgcgcaa atgactcgtc aattttacca tgatccaaat
     2341 catattgaat atagatatcc aggaatgaag aaacgttgcc caatgacata gctgctccat
     2401 cttgttcttt cacggcagca agatatgcca tataaaccca ctgaacagct tcttgtgcat
     2461 tagtagcggg acgacccaag tcgagtccgt aatattcacc caatatcttt atttctttca
     2521 gtgctttgat ttgttcggct acttcttcgc gcaggcggat acgggcatcg gtcatcggac
     2581 tggtgatatt gtgcaaatct tcttgttttg cttcaatcag tcggtcgata ccatacagtg
     2641 ccaaacggcg gtagtcaccg atgatacgcc cgcgtgcata attatcgggc agaccggtca
     2701 ggaaaccgag tgaacggaaa gagcggattt cttcggtgta agcatcaaat acgccatcat
     2761 tatgggtttt gcgataatga gtaaagatat cctttacttt ttcatctact tccacaccgt
     2821 tttcatggca ggctttttct acgactttga taccaccata aggtttgatg gcacggcgca
     2881 gaagttcatc cgtttgaaga cctacaatca gttcgttttc acggtcaatg tatcctgctt
     2941 tatgtgaggt gatggtagat actgttacat tatcaaggga acgaactccg ttattggcac
     3001 gttcttcttc tagtgccttg aggcattcgt tccacacttt tttagttcgc tctgtggggc
     3061 ctgccagaaa tgaggcatcg ccctcataag gggtaatgtt ggtatgtacg aagtctgaaa
     3121 cattaatttc cttactccac aatccatcct taaaaacttt gttcatttcc atactgttat
     3181 tccattatta agagatttag ggctgcaaag gtactccttt tttgtgagct atgcatcact
     3241 attagtaggt ataatgcgaa tacttgtgca tttttaagta taaagtggtc aataaatgaa
     3301 aaaacaataa ggactcttta taacaattcc ataaaaaata aagctaattg gtgacaataa
     3361 accaattagc ttctaaaagt agcggaaccg ggaatcgaac ccgagtttca gccgtgagag
     3421 ggctgcgtcc taggccacta gacgataccg ccatcagtta gtcttaattc tttttctaag
     3481 acggtgcaaa gttagaagtt tgtttttaat ctgcaaaata aaaaatgatt ttttttatgc
     3541 tattactatt ataaatcgga atttaatttc tatgaagaag tatcatatgt tttatgtagt
     3601 aaaattgaaa gtctaaaaag gggtcaacgt aaaatcctga ggggtatatc cgataaaatc
     3661 ctatctttgt
//
