GCA_020560295.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaMHLMBBNI_1 (original name was: gnl|Prokka|MHLMBBNI_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	NRPS,cyclic-lactone-autoinducer	1	50,558	ulbactin F/ulbactin G	NRP	28%

gnlProkkaMHLMBBNI_2 (original name was: gnl|Prokka|MHLMBBNI_2)

Region	Type	From	To	Most similar known cluster		Similarity
Region 2.1	ranthipeptide	25,324	46,912

gnlProkkaMHLMBBNI_75 (original name was: gnl|Prokka|MHLMBBNI_75)

Region	Type	From	To	Most similar known cluster		Similarity
Region 13.1	NRPS	19,728	68,712	dipeptide aldehydes	NRP	94%

gnlProkkaMHLMBBNI_85 (original name was: gnl|Prokka|MHLMBBNI_85)

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	lanthipeptide-class-ii	187,098	200,947

gnlProkkaMHLMBBNI_151 (original name was: gnl|Prokka|MHLMBBNI_151)

Region	Type	From	To	Most similar known cluster		Similarity
Region 88.1	cyclic-lactone-autoinducer	122,023	132,824

Compact view

gnlProkkaMHLMBBNI_1 - Region 1 - NRPS,cyclic-lactone-autoinducer

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Location: 1 - 50,558 nt. (total: 50,558 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 50558:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - Cys) + (Me-Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg1_22

Search NORINE for peptide: strict or relaxed

AMP-binding (527..921): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNLSLIWK
Nearest Stachelhaus code(s):: Cys DLFNLSLIWK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_23

Search NORINE for peptide: strict or relaxed

AMP-binding (553..948): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSMIWK
Nearest Stachelhaus code(s):: Cys DLYNMSMIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1974..2374): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNMSLIWK
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg1_24

Search NORINE for peptide: strict or relaxed

AMP-binding (25..431): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: PLPAQGVLSK
Nearest Stachelhaus code(s):: diOH-Bz PLPAQGVLCK (79% 8Å match); Sal PLPAQGVLNK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

gnlProkkaMHLMBBNI_2 - Region 1 - ranthipeptide

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Location: 25,324 - 46,912 nt. (total: 21,589 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaMHLMBBNI_75 - Region 1 - NRPS

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Location: 19,728 - 68,712 nt. (total: 48,985 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 19727 - 68712:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg13_39

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaMHLMBBNI_85 - Region 1 - lanthipeptide-class-ii

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Location: 187,098 - 200,947 nt. (total: 13,850 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

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KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg23_161

ctg23_160

Leader:

MKNNEKNFIGDINKELEEIEKLDILEEIEESLLTMLGS

Core:

VNSLFCCH

Core with dehydrations:

VNDhaLFCysCysH

allorf_188596_188709

Leader:

MTCCLMVMNIRKALSAG

Core:

KIHPTERASVFCPYFLVSGL

Core with dehydrations:

KIHPDhbERADhaVFCysPYFLVDhaGL

antiSMASH-DB 4.0 matches for allorf_188596_188709

100.0% NZ_JADMYT010000009.1and 3 others:

NZ_JADNMI010000013.1 Lanthipeptides: allorf_010454_010567_lanthipeptideNZ_QUEF01000006.1 Lanthipeptides: allorf_116827_116940_lanthipeptideNZ_QUIT01000007.1 Lanthipeptides: allorf_004751_004864_lanthipeptide

Lanthipeptides: allorf_115889_116002_lanthipeptide

1

KIHPTERASVFCPYFLVSGL

20

KIHPTERASVFCPYFLVSGL

1

KIHPTERASVFCPYFLVSGL

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg23_160 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 903.4 Da

Molecular weight: 904.1 Da

Number of bridges: 1

allorf_188596_188709 - Class II

Cleavage pHMM score: -5.50

RODEO score: 0

Monoisotopic mass: 2209.2 Da

Molecular weight: 2210.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2228.6 Da N = 1; 2246.6 Da N = 2

gnlProkkaMHLMBBNI_151 - Region 1 - cyclic-lactone-autoinducer

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Location: 122,023 - 132,824 nt. (total: 10,802 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.