GCA_020563945.1 - 11 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaCGDIBNIP_1 (original name was: gnl|Prokka|CGDIBNIP_1)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1.1	cyclic-lactone-autoinducer	121,333	142,082

gnlProkkaCGDIBNIP_12 (original name was: gnl|Prokka|CGDIBNIP_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 3.1	ranthipeptide	14,848	36,436

gnlProkkaCGDIBNIP_101 (original name was: gnl|Prokka|CGDIBNIP_101)

Region	Type	From	To	Most similar known cluster		Similarity
Region 17.1	NRPS	25,334	60,356	dipeptide aldehydes	NRP	47%

gnlProkkaCGDIBNIP_107 (original name was: gnl|Prokka|CGDIBNIP_107)

Region	Type	From	To	Most similar known cluster		Similarity
Region 23.1	NRPS	166,632	214,543

gnlProkkaCGDIBNIP_112 (original name was: gnl|Prokka|CGDIBNIP_112)

Region	Type	From	To	Most similar known cluster		Similarity
Region 28.1	lanthipeptide-class-ii	7,790	30,780

gnlProkkaCGDIBNIP_118 (original name was: gnl|Prokka|CGDIBNIP_118)

Region	Type	From	To	Most similar known cluster		Similarity
Region 34.1	lanthipeptide-class-ii	147,687	170,347

gnlProkkaCGDIBNIP_122 (original name was: gnl|Prokka|CGDIBNIP_122)

Region	Type	From	To	Most similar known cluster		Similarity
Region 38.1	cyclic-lactone-autoinducer	1	17,425

gnlProkkaCGDIBNIP_126 (original name was: gnl|Prokka|CGDIBNIP_126)

Region	Type	From	To	Most similar known cluster		Similarity
Region 42.1	RRE-containing	1	15,229

gnlProkkaCGDIBNIP_130 (original name was: gnl|Prokka|CGDIBNIP_130)

Region	Type	From	To	Most similar known cluster		Similarity
Region 46.1	NRPS	1	18,897	equibactin	NRP	14%

gnlProkkaCGDIBNIP_162 (original name was: gnl|Prokka|CGDIBNIP_162)

Region	Type	From	To	Most similar known cluster		Similarity
Region 78.1	cyclic-lactone-autoinducer	1	12,871

gnlProkkaCGDIBNIP_173 (original name was: gnl|Prokka|CGDIBNIP_173)

Region	Type	From	To	Most similar known cluster		Similarity
Region 89.1	cyclic-lactone-autoinducer	107,620	124,295

Region	Type	From	To	Most similar known cluster	Similarity
Region 1.1	cyclic-lactone-autoinducer	121,333	142,082
Region 3.1	ranthipeptide	14,848	36,436
Region 17.1	NRPS	25,334	60,356	dipeptide aldehydes	NRP	47%
Region 23.1	NRPS	166,632	214,543
Region 28.1	lanthipeptide-class-ii	7,790	30,780
Region 34.1	lanthipeptide-class-ii	147,687	170,347
Region 38.1	cyclic-lactone-autoinducer	1	17,425
Region 42.1	RRE-containing	1	15,229
Region 46.1	NRPS	1	18,897	equibactin	NRP	14%
Region 78.1	cyclic-lactone-autoinducer	1	12,871
Region 89.1	cyclic-lactone-autoinducer	107,620	124,295

No secondary metabolite regions were found in these records:: gnlProkkaCGDIBNIP_2 (original name was: gnl|Prokka|CGDIBNIP_2); gnlProkkaCGDIBNIP_22 (original name was: gnl|Prokka|CGDIBNIP_22); gnlProkkaCGDIBNIP_33 (original name was: gnl|Prokka|CGDIBNIP_33); gnlProkkaCGDIBNIP_40 (original name was: gnl|Prokka|CGDIBNIP_40); gnlProkkaCGDIBNIP_51 (original name was: gnl|Prokka|CGDIBNIP_51); gnlProkkaCGDIBNIP_62 (original name was: gnl|Prokka|CGDIBNIP_62); gnlProkkaCGDIBNIP_73 (original name was: gnl|Prokka|CGDIBNIP_73); gnlProkkaCGDIBNIP_84 (original name was: gnl|Prokka|CGDIBNIP_84); gnlProkkaCGDIBNIP_95 (original name was: gnl|Prokka|CGDIBNIP_95); gnlProkkaCGDIBNIP_96 (original name was: gnl|Prokka|CGDIBNIP_96); gnlProkkaCGDIBNIP_97 (original name was: gnl|Prokka|CGDIBNIP_97); gnlProkkaCGDIBNIP_98 (original name was: gnl|Prokka|CGDIBNIP_98); gnlProkkaCGDIBNIP_99 (original name was: gnl|Prokka|CGDIBNIP_99); gnlProkkaCGDIBNIP_100 (original name was: gnl|Prokka|CGDIBNIP_100); gnlProkkaCGDIBNIP_102 (original name was: gnl|Prokka|CGDIBNIP_102); gnlProkkaCGDIBNIP_103 (original name was: gnl|Prokka|CGDIBNIP_103); gnlProkkaCGDIBNIP_104 (original name was: gnl|Prokka|CGDIBNIP_104); gnlProkkaCGDIBNIP_105 (original name was: gnl|Prokka|CGDIBNIP_105); gnlProkkaCGDIBNIP_106 (original name was: gnl|Prokka|CGDIBNIP_106); gnlProkkaCGDIBNIP_108 (original name was: gnl|Prokka|CGDIBNIP_108); gnlProkkaCGDIBNIP_109 (original name was: gnl|Prokka|CGDIBNIP_109); gnlProkkaCGDIBNIP_110 (original name was: gnl|Prokka|CGDIBNIP_110); gnlProkkaCGDIBNIP_111 (original name was: gnl|Prokka|CGDIBNIP_111); gnlProkkaCGDIBNIP_113 (original name was: gnl|Prokka|CGDIBNIP_113); gnlProkkaCGDIBNIP_114 (original name was: gnl|Prokka|CGDIBNIP_114); gnlProkkaCGDIBNIP_115 (original name was: gnl|Prokka|CGDIBNIP_115); gnlProkkaCGDIBNIP_116 (original name was: gnl|Prokka|CGDIBNIP_116); gnlProkkaCGDIBNIP_117 (original name was: gnl|Prokka|CGDIBNIP_117); gnlProkkaCGDIBNIP_119 (original name was: gnl|Prokka|CGDIBNIP_119); gnlProkkaCGDIBNIP_120 (original name was: gnl|Prokka|CGDIBNIP_120); gnlProkkaCGDIBNIP_121 (original name was: gnl|Prokka|CGDIBNIP_121); gnlProkkaCGDIBNIP_123 (original name was: gnl|Prokka|CGDIBNIP_123); gnlProkkaCGDIBNIP_124 (original name was: gnl|Prokka|CGDIBNIP_124); gnlProkkaCGDIBNIP_125 (original name was: gnl|Prokka|CGDIBNIP_125); gnlProkkaCGDIBNIP_127 (original name was: gnl|Prokka|CGDIBNIP_127); gnlProkkaCGDIBNIP_128 (original name was: gnl|Prokka|CGDIBNIP_128); gnlProkkaCGDIBNIP_129 (original name was: gnl|Prokka|CGDIBNIP_129); gnlProkkaCGDIBNIP_131 (original name was: gnl|Prokka|CGDIBNIP_131); gnlProkkaCGDIBNIP_132 (original name was: gnl|Prokka|CGDIBNIP_132); gnlProkkaCGDIBNIP_133 (original name was: gnl|Prokka|CGDIBNIP_133); gnlProkkaCGDIBNIP_134 (original name was: gnl|Prokka|CGDIBNIP_134); gnlProkkaCGDIBNIP_135 (original name was: gnl|Prokka|CGDIBNIP_135); gnlProkkaCGDIBNIP_136 (original name was: gnl|Prokka|CGDIBNIP_136); gnlProkkaCGDIBNIP_137 (original name was: gnl|Prokka|CGDIBNIP_137); gnlProkkaCGDIBNIP_138 (original name was: gnl|Prokka|CGDIBNIP_138); gnlProkkaCGDIBNIP_139 (original name was: gnl|Prokka|CGDIBNIP_139); gnlProkkaCGDIBNIP_140 (original name was: gnl|Prokka|CGDIBNIP_140); gnlProkkaCGDIBNIP_141 (original name was: gnl|Prokka|CGDIBNIP_141); gnlProkkaCGDIBNIP_142 (original name was: gnl|Prokka|CGDIBNIP_142); gnlProkkaCGDIBNIP_143 (original name was: gnl|Prokka|CGDIBNIP_143); gnlProkkaCGDIBNIP_144 (original name was: gnl|Prokka|CGDIBNIP_144); gnlProkkaCGDIBNIP_145 (original name was: gnl|Prokka|CGDIBNIP_145); gnlProkkaCGDIBNIP_146 (original name was: gnl|Prokka|CGDIBNIP_146); gnlProkkaCGDIBNIP_147 (original name was: gnl|Prokka|CGDIBNIP_147); gnlProkkaCGDIBNIP_148 (original name was: gnl|Prokka|CGDIBNIP_148); gnlProkkaCGDIBNIP_149 (original name was: gnl|Prokka|CGDIBNIP_149); gnlProkkaCGDIBNIP_150 (original name was: gnl|Prokka|CGDIBNIP_150); gnlProkkaCGDIBNIP_151 (original name was: gnl|Prokka|CGDIBNIP_151); gnlProkkaCGDIBNIP_152 (original name was: gnl|Prokka|CGDIBNIP_152); gnlProkkaCGDIBNIP_153 (original name was: gnl|Prokka|CGDIBNIP_153); gnlProkkaCGDIBNIP_154 (original name was: gnl|Prokka|CGDIBNIP_154); gnlProkkaCGDIBNIP_155 (original name was: gnl|Prokka|CGDIBNIP_155); gnlProkkaCGDIBNIP_156 (original name was: gnl|Prokka|CGDIBNIP_156); gnlProkkaCGDIBNIP_157 (original name was: gnl|Prokka|CGDIBNIP_157); gnlProkkaCGDIBNIP_158 (original name was: gnl|Prokka|CGDIBNIP_158); gnlProkkaCGDIBNIP_159 (original name was: gnl|Prokka|CGDIBNIP_159); gnlProkkaCGDIBNIP_160 (original name was: gnl|Prokka|CGDIBNIP_160); gnlProkkaCGDIBNIP_161 (original name was: gnl|Prokka|CGDIBNIP_161); gnlProkkaCGDIBNIP_163 (original name was: gnl|Prokka|CGDIBNIP_163); gnlProkkaCGDIBNIP_164 (original name was: gnl|Prokka|CGDIBNIP_164); gnlProkkaCGDIBNIP_165 (original name was: gnl|Prokka|CGDIBNIP_165); gnlProkkaCGDIBNIP_166 (original name was: gnl|Prokka|CGDIBNIP_166); gnlProkkaCGDIBNIP_167 (original name was: gnl|Prokka|CGDIBNIP_167); gnlProkkaCGDIBNIP_168 (original name was: gnl|Prokka|CGDIBNIP_168); gnlProkkaCGDIBNIP_169 (original name was: gnl|Prokka|CGDIBNIP_169); gnlProkkaCGDIBNIP_170 (original name was: gnl|Prokka|CGDIBNIP_170); gnlProkkaCGDIBNIP_171 (original name was: gnl|Prokka|CGDIBNIP_171); gnlProkkaCGDIBNIP_172 (original name was: gnl|Prokka|CGDIBNIP_172); gnlProkkaCGDIBNIP_174 (original name was: gnl|Prokka|CGDIBNIP_174)

Compact view

gnlProkkaCGDIBNIP_1 - Region 1 - cyclic-lactone-autoinducer

Download region GenBank file

Download region SVG

Location: 121,333 - 142,082 nt. (total: 20,750 nt) Show pHMM detection rules used

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCGDIBNIP_12 - Region 1 - ranthipeptide

Download region GenBank file

Download region SVG

Location: 14,848 - 36,436 nt. (total: 21,589 nt) Show pHMM detection rules used

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCGDIBNIP_101 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 25,334 - 60,356 nt. (total: 35,023 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 25333 - 60356:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg17_47

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaCGDIBNIP_107 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 166,632 - 214,543 nt. (total: 47,912 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 166631 - 214543:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - Cys) + (Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg23_181

Search NORINE for peptide: strict or relaxed

AMP-binding (531..918): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNMSLIWK
Nearest Stachelhaus code(s):: Cys DLFNMSLIWK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg23_182

Search NORINE for peptide: strict or relaxed

AMP-binding (544..940): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNFSMIWK
Nearest Stachelhaus code(s):: Cys DLYNFSMIWK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1978..2370): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNLSLIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

ctg23_183

AMP-binding (9..404): X

nrpys: diOH-Bz, Sal

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: N/A
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AFPSQGLVCK
Nearest Stachelhaus code(s):: n-oh-nitropheBut TLPSQGVVCK (65% 8Å match)
Stachelhaus code match:: 70% (weak)

gnlProkkaCGDIBNIP_112 - Region 1 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 7,790 - 30,780 nt. (total: 22,991 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg28_23

ctg28_22

Leader:

MFAEFEKEEMLNDEDLYINAM

Core:

NNDSASSYASNVSDSCCC

Core with dehydrations:

NNDDhaADhaDhaYADhaNVDhaDDhaCysCysCys

antiSMASH-DB 4.0 matches for ctg28_22

28.0% NZ_AP023438.1and 1 other:

NZ_AP023438.1 Lanthipeptides: IGA62_RS12300_lanthipeptide

Lanthipeptides: IGA62_RS12300_lanthipeptide

5

ASSYASNVSDSC

16

AS YA+N D C

1

ASGYATNTDDGC

12

allorf_08197_08370

Leader:

MFCSCIHGCSKFNRKLFDERENNRFNAADILYA

Core:

IQAICVAFLKTALIYYLIAIFFRR

Core with dehydrations:

IQAICysVAFLKDhbALIYYLIAIFFRR

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg28_22 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 1717.6 Da

Molecular weight: 1718.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 1736.8 Da N = 1; 1754.8 Da N = 2; 1772.8 Da N = 3

allorf_08197_08370 - Class II

Cleavage pHMM score: -0.60

RODEO score: 0

Monoisotopic mass: 2827.6 Da

Molecular weight: 2829.5 Da

Number of bridges: 1

gnlProkkaCGDIBNIP_118 - Region 1 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 147,687 - 170,347 nt. (total: 22,661 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg34_131

ctg34_121

Leader:

MGDKRLYKSSENSMLCGVCGG

Core:

IAEYFGIDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

Core with dehydrations:

IAEYFGIDPDhbLVRLAWVILDhbCysFGGAGIWAYIIAAIIIPKR

MIBiG 3.1 matches for ctg34_121

12.5% BGC0000517 (BAB04173.1): Lanthipeptide: haloduracin β or haloduracin α

14

LAWVILTC-FGGAGIWAYIIAAII

36

AW ++C G G + + +

1

CAWYNISCRLGNKGAYCTLTVECM

24

antiSMASH-DB 4.0 matches for ctg34_121

97.5% NZ_CZBP01000025.1: Lanthipeptides: ARA22_RS13900_lanthipeptide

1

IAEYFGIDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

40

IAEYF IDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

1

IAEYFDIDPTLVRLAWVILTCFGGAGIWAYIIAAIIIPKR

40

14.3% NZ_MOWR01000001.1: Lanthipeptides: BMI76_RS01735_lanthipeptide

16

WVILTCFGGAGIWA

29

+V L C GAG++A

28

YVTLACAAGAGVYA

41

ctg34_130

Leader:

MKNNEKNFIGDINKELEEIEKLDILEEIEESLLTMLGS

Core:

VNSLFCCH

Core with dehydrations:

VNDhaLFCysCysH

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg34_121 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 4367.5 Da

Molecular weight: 4370.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4388.3 Da N = 1

ctg34_130 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 903.4 Da

Molecular weight: 904.1 Da

Number of bridges: 1

gnlProkkaCGDIBNIP_122 - Region 1 - cyclic-lactone-autoinducer

Download region GenBank file

Download region SVG

Location: 1 - 17,425 nt. (total: 17,425 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCGDIBNIP_126 - Region 1 - RRE-containing

Download region GenBank file

Download region SVG

Location: 1 - 15,229 nt. (total: 15,229 nt) Show pHMM detection rules used

Region on contig edge.

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCGDIBNIP_130 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 18,897 nt. (total: 18,897 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 18897:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys) + (Me-Cys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg46_9

Search NORINE for peptide: strict or relaxed

AMP-binding (526..926): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLANISQIWK
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (74% 8Å match)
Stachelhaus code match:: 70% (weak)

ctg46_10

Search NORINE for peptide: strict or relaxed

AMP-binding (557..957): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNMSMIWK
Nearest Stachelhaus code(s):: Cys DLYNMSMIWK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

gnlProkkaCGDIBNIP_162 - Region 1 - cyclic-lactone-autoinducer

Download region GenBank file

Download region SVG

Location: 1 - 12,871 nt. (total: 12,871 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaCGDIBNIP_173 - Region 1 - cyclic-lactone-autoinducer

Download region GenBank file

Download region SVG

Location: 107,620 - 124,295 nt. (total: 16,676 nt) Show pHMM detection rules used

Region on contig edge.

cyclic-lactone-autoinducer: (TIGR04223 or AgrB)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.