GCA_020687085.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaHGCANGJP_10 (original name was: gnl|Prokka|HGCANGJP_10)

Region	Type	From	To	Most similar known cluster		Similarity
Region 10.1	lanthipeptide-class-ii	1	5,726

gnlProkkaHGCANGJP_55 (original name was: gnl|Prokka|HGCANGJP_55)

Region	Type	From	To	Most similar known cluster		Similarity
Region 55.1	NRPS	19,074	55,937	dipeptide aldehydes	NRP	58%

gnlProkkaHGCANGJP_62 (original name was: gnl|Prokka|HGCANGJP_62)

Region	Type	From	To	Most similar known cluster		Similarity
Region 62.1	ranthipeptide	28,361	47,464

gnlProkkaHGCANGJP_74 (original name was: gnl|Prokka|HGCANGJP_74)

Region	Type	From	To	Most similar known cluster		Similarity
Region 74.1	RRE-containing	6,318	27,736

gnlProkkaHGCANGJP_87 (original name was: gnl|Prokka|HGCANGJP_87)

Region	Type	From	To	Most similar known cluster		Similarity
Region 87.1	lanthipeptide-class-ii	2,446	25,436

Region	Type	From	To	Most similar known cluster	Similarity
Region 10.1	lanthipeptide-class-ii	1	5,726
Region 55.1	NRPS	19,074	55,937	dipeptide aldehydes	NRP	58%
Region 62.1	ranthipeptide	28,361	47,464
Region 74.1	RRE-containing	6,318	27,736
Region 87.1	lanthipeptide-class-ii	2,446	25,436

Compact view

gnlProkkaHGCANGJP_10 - Region 1 - lanthipeptide-class-ii

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Location: 1 - 5,726 nt. (total: 5,726 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg10_3

ctg10_4

Leader:

MNEMNDKIIGNVEKELDEIEIIDTESIEEKLSFIVYG

Core:

TIVCC

Core with dehydrations:

DhbIVCysCys

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg10_4 - Class II: Cleavage pHMM score: -5.90; RODEO score: 0; Monoisotopic mass: 519.2 Da; Molecular weight: 519.7 Da; Number of bridges: 1

gnlProkkaHGCANGJP_55 - Region 1 - NRPS

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Location: 19,074 - 55,937 nt. (total: 36,864 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 19073 - 55937:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg55_40

AMP-binding (20..419): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVLTDGGISK
Nearest Stachelhaus code(s):: Leu or Trp DVLTDGGISK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1033..1421): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMFSGAIFK
Nearest Stachelhaus code(s):: Leu or Trp DAMFSGAIFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2027..2418): X

nrpys: Leu, Trp

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAITTGGVSK
Nearest Stachelhaus code(s):: Leu or Trp DAITTGGVSK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaHGCANGJP_62 - Region 1 - ranthipeptide

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Location: 28,361 - 47,464 nt. (total: 19,104 nt) Show pHMM detection rules used

Region on contig edge.

ranthipeptide: (TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085 or SPASM)) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716 or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or (YcaO or TIGR03604))))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaHGCANGJP_74 - Region 1 - RRE-containing

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Location: 6,318 - 27,736 nt. (total: 21,419 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaHGCANGJP_87 - Region 1 - lanthipeptide-class-ii

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Location: 2,446 - 25,436 nt. (total: 22,991 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

KnownClusterBlast

Lanthipeptides

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

No matches found.

Lanthipeptide predictions

Lanthipeptide(s) for ctg87_14

ctg87_15

Leader:

MFAEFEKEEMLNDEDLYINAM

Core:

NNDSASSYASNVSDSCCC

Core with dehydrations:

NNDDhaADhaDhaYADhaNVDhaDDhaCysCysCys

antiSMASH-DB 4.0 matches for ctg87_15

28.0% NZ_AP023438.1and 1 other:

NZ_AP023438.1 Lanthipeptides: IGA62_RS12300_lanthipeptide

Lanthipeptides: IGA62_RS12300_lanthipeptide

5

ASSYASNVSDSC

16

AS YA+N D C

1

ASGYATNTDDGC

12

allorf_24856_25029

Leader:

MFCSCIHGCSKFNRKLFDERENNRFNAADILYA

Core:

IQAICVAFLKTALIYYLIAIFFRR

Core with dehydrations:

IQAICysVAFLKDhbALIYYLIAIFFRR

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Lanthipeptides

Core peptide predictions

ctg87_15 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 1717.6 Da

Molecular weight: 1718.7 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 1736.8 Da N = 1; 1754.8 Da N = 2; 1772.8 Da N = 3

allorf_24856_25029 - Class II

Cleavage pHMM score: -0.60

RODEO score: 0

Monoisotopic mass: 2827.6 Da

Molecular weight: 2829.5 Da

Number of bridges: 1