LOCUS       c00022_gnlPro..         5109 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LOJFJKPI_22.
ACCESSION   c00022_gnlPro..
VERSION     c00022_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 06:18:01
            Original ID  :: gnl|Prokka|LOJFJKPI_22
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5109
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5109
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[326:3297]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      327..3297
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5109
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5109
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             327..2555
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg22_1"
                     /sec_met_domain="Gly_radical (E-value: 4.4e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1174.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLRRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2569..3297
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.7;
                     E-value: 6.1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg22_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3290..3739)
                     /locus_tag="ctg22_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3726..3893)
                     /locus_tag="ctg22_4"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDKVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(3953..4456)
                     /locus_tag="ctg22_5"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVLTRAVAQAGAGDDGGEDPDKNPGSGGDGSGDEEAP
                     DPTV"
     CDS             4650..4880
                     /locus_tag="ctg22_6"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             4967..5107
                     /locus_tag="ctg22_7"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GE"
ORIGIN
        1 gcggagagac agggattcga accccgggta cctcgcagta caacggtttt caagaccgcc
       61 gcaatcgacc actctgccac ctctccaaaa cttccttatc agaagtgctt ttcgttaaaa
      121 gcggtgcaaa ggtacaaaga tattttaaac ccgcaagagt tttcactaaa aatttaatct
      181 aatcttttcc taaggatagc aattaatagg tattttatgt tgaaaagcat aaaatagagc
      241 atatttatgc cgattatatg aaaaatgatt gtaactttgt gcgctctaaa gaataggata
      301 actacaacct ctaattttat cattttatgg aattaaacaa gatctttaaa gacggtcttt
      361 ggagcagcga aatcaacgtc agagatttcg taagtcataa catcactccg tattacggag
      421 atgcttcatt cctcgaagga cctacagaac gtaccaaagc cgtatggaac cgctgcctcg
      481 aagcgttggc agaagaaaga gaaaacaacg gtgtccgctc attagataat gttaccgtat
      541 caaccatcac ttcccacaag gccggatata tcgacaagga aaacgaactg atcgtcggcc
      601 tgcagacaga cgaacttctg agacgtgcca tcaagccttt cggaggtatc aacgtagtca
      661 gcaaagcctg tcacgaaaac ggtgtggaag tggacgaccg cgtaaaagat attttcactc
      721 actaccgcaa gacgcacaac gacggagtat tcgacgtgta tacggaagaa atccgttcct
      781 tccgttcgct gggattcctc accggacttc ccgacaatta tgcacgcgga cgtatcatcg
      841 gtgactaccg ccgtatggct ctttacggca tcgaccgtct gatcgaagca aagaaggaag
      901 atttgcataa cctcaccggt ccgatgacag aggcccgtat ccgtctgcgc gaagaagtgg
      961 cagaacagat caaggcactg aaagacatga aagtgatggg cgaatactac ggtctcgacc
     1021 tgagccgtcc cgcttacacg gcacaagaag ccgtacagtg ggtatatatg gcttaccttg
     1081 ccgccgtcaa agaacaagac ggtgccgcca tgtcactggg taacgtttct tctttcctcg
     1141 atatctatct ggaatatgaa ctgagcaaag gaaccatcac cgaatcgttc gcgcaagagc
     1201 tgatcgacca gtttgtcatc aaactgcgta tggtccgcca cctgcgtatg caatcataca
     1261 acgacatctt tgcaggcgac ccgacctggg taaccgaatc tctgggcgga cgtctcaacg
     1321 acggacgtac caaagtgaca aagacctcct tccgtttcct gcagacattg tacaacctcg
     1381 gcccttcacc ggaaccgaac ctgaccgtac tctggagccc ggaacttccc gaaggattca
     1441 aagaattctg tgcaaaggtt tctatcgaca cttcttctat ccagtacgaa aacgacgacc
     1501 tgatgcgtga agtacgtcag tccgacgact acggaatcgc ctgctgcgta tcataccagg
     1561 aaataggcaa gcagattcag ttcttcggtg cccgttgcaa cctggccaaa gccctgttgc
     1621 ttgccatcaa cggcggacgt tgcgagaata ccggtacagt catggtgaag aacatccccg
     1681 tactgaccag tgacacgctg aagttcgaag aagtaatgga caactacaag aaagtgttga
     1741 ccgaaatcgc ccgcgtatac aacgaagcga tgaacatcat ccactatatg cacgacaagt
     1801 attactacga gaaagcccag atggctcttg tagatacgaa cccgcgcata aaccttgctt
     1861 acggtgttgc cggactttcc atcgcactcg actcactgtc tgccatcaaa tatgcgaaag
     1921 taactgcccg ccgcaacgat atcggcctga cagaaggttt cgacatcgaa ggagaattcc
     1981 cttgcttcgg taacgacaac gacaaggtag accaccttgg cgtagacctg gtatacttct
     2041 tcagcgaaga attgaagaaa ttgcctgttt acaagaatgc ccgtcctacc ctctccttgc
     2101 tgactatcac ttccaatgtg atgtacggca aaaagaccgg tgctactccc gacggacgtg
     2161 ccaaaggtgt tgccttcgct ccgggtgcca acccgatgca cggacgcgac aagaatggtg
     2221 cgatcgcttc tctgagctct gtagcgaaac ttcgttaccg tgactcacag gacggtatca
     2281 gcaacacctt ctctatcgtt ccgaaatcac tgggtgctac ggacgaagac cgtatcgaga
     2341 acctcgtaac gatgatggac ggttacttca ccaaaggtgc ccaccacctg aacgtaaacg
     2401 tactgaaccg tgatatgcta tacgatgcca tggagcatcc ggagaactat ccgcagctga
     2461 ctatccgagt ttccggttat gccgtaaact tcgtgaagct gagccgcgaa caccaactgg
     2521 aagttatcag ccgtagcttc cacgaacgta tgtaatctat tactctatat gatgataaac
     2581 gtacattcat acgaaagtat gggaacattc gacgggccgg gcttacggct cgtcgttttt
     2641 cttcaaggat gcaacttccg ctgcctctat tgcgccaatc cggatacgat agcaggaaag
     2701 ggaggcacac ctaccccacc ggaagaaatc gtccgtatgg caatgagcca gcgccctttc
     2761 ttcgggaaac gcggaggaat cactttctcc ggaggcgaac cgacgtttca ggcgaaagca
     2821 ctcgtcccgc tggtccgcga actgaaagaa aaaggcattc atgtctgtct ggacagcaac
     2881 ggaggacttt ggaacgaaga cgtagaagaa ttattcaagc tgaccgacct tgtgttattg
     2941 gatataaagg aattcaaccc caaccgccat cagacgctga ccggaagaag caatgaacag
     3001 accatccgta cggccgcctg gctggaagaa cagggaaaac ctttctggct gcgttatgtg
     3061 ctagtgcccg gatacagcga ttttgaagaa gatatacgag cactgggaga agcactcgga
     3121 aaatacaaga tgatccaacg ggtggaaata ctgccttacc atacactggg cgtacataag
     3181 tacgaagcga tgggacagga atacaagatg aaaggggtaa aagaaaatac ccccgaacag
     3241 cttgaaaaag ctgcggaggt attcaaagag tatttcacta cggtggtagt taattaatgg
     3301 agaattctcc aatgacctca aagcaagggc attctttaat ccgttcccaa ggatctacaa
     3361 tcccattata gttggtgtcg ggacttaagt cccgatgacc tactacctta gcttccggat
     3421 aacgctgcaa caattcgtgt accaaacgat gtaacgcctc cttctgacgg ggtgtacgcg
     3481 tatccgccgc ttttccattc tcatccaaac cgccctcata gcatacacct aaactgatcg
     3541 cattgtagcc acgtgcatga gccccgaccg tatcttcatc ccgcatcggc tcaatctcgc
     3601 ccgacttgcg tatataataa tgatagcccc agcacgaaaa gccacgataa cggtgagccg
     3661 tatccacatc ctttgccgta aagtcgcaat cgacacgggt ggcggaacaa tggatcacga
     3721 tcagactaat ctttctcatg ttccttttcc ctttccgtcg ttttcacttt gcacacctcc
     3781 ttacaagtag tatgatagcg cacttctctc accaccttgt cccgctttct ctttccgaag
     3841 aaaggaatca gatacatgat cacatctaaa atccggttta ctatttcttt cattttatca
     3901 aaatattaat ggaagttatt ctaattcgtt attaccttga cccgtctgcc aattataccg
     3961 tagggtccgg agcctcttca tcgccggagc catcaccgcc ggaaccgggg ttcttgtcag
     4021 ggtcttctcc cccgtcatcg cccgcacccg cctgcgccac cgcacgagtc agcacttgct
     4081 gataactcag aatcttcatc aactccgcca gcgtcttacc ggggcggaaa ataagatgcg
     4141 cccccttaat catcgaaccg gaaaactcct tgaccgatgc cgcaccttca ctgttcagcc
     4201 ctatctgcaa actgcccaaa tcgcccagtc tcgcaattct tcctgccttc aacgcacgct
     4261 tcacttcgta aagaataccc gaaatagccg cacgtacatc cgtttccgta caagtagaac
     4321 gactggtgat cgcttcgcac aactcttcaa agtccaactc ccccgacgcc tgcgcctgtg
     4381 cataatactt tgccggttcg ctcttttttg caggattctt gcgcggcacc actgaataag
     4441 atactgtcat tgccataata aaataatttt aaagtttata atacgtagga tgcatccctt
     4501 tcttgttaac agcacaaaga taagggtatc cggacaagcc atattcccca acgaagtttc
     4561 ttgatttcca acgaataaaa acgaagtcaa acctttgaaa aacaataaat tttatgtaac
     4621 tttatagcat aaaacaagaa gaaaacatca tgaattacaa agaagattct ttccttatcc
     4681 gcacctatac caaagcggaa ctagctcacc tttacaatcc ccatgtctgc ctcaaagttg
     4741 ctttacaaat acttcgtcgt tggattattt acaacctccc tctcctgcac gaactcgaac
     4801 aggaaggcta ccgcgcacgc aaccgtctcc tttcgccgaa acaagtggca actatcatac
     4861 actttttggg cgaaccgtag taacttattg ccattttata caaagttcct ttacacattt
     4921 ccgcaaagaa gttcctttgc atcgacaaac aataatcaaa ataaaaatga aacatttcac
     4981 catatttcaa ggtttctact acgcaatagc agagatgacc gaagaagaaa tagtgtccac
     5041 tatcggcagt tttacctacc gggaaaaagt cgaagaaata cgccgcatct ttgcagagca
     5101 aggagaaaa
//
