LOCUS       c00353_gnlPro..         3664 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PIJEIODF_353.
ACCESSION   c00353_gnlPro..
VERSION     c00353_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 10:37:43
            Original ID  :: gnl|Prokka|PIJEIODF_353
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3664
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3664
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[103:3617]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      104..3617
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3664
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3664
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             104..808
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg353_1"
                     /sec_met_domain="SucD_FumR (E-value: 2e-131, bitscore:
                     423.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLSNSSVGRKVVMSVTGIALVLFLTFHMAMNLVAIISADGYNMI
                     CEFLGANWYALVATAALAALFIIHIIYAFWLTMQNRTARGSERYAVVDKPKTVEWASQN
                     MLVLGIIVIVGLGLHLFNFWAKMQLPELMHNLGMHADTLTLAYAANGAYHIQQTFSCPV
                     YVVLYLIWLFALWLHLTHGFWSSMQSLGWNNKVWINRWKCISNIYSTIVVLGFALVVVV
                     FFVKSLLCGGAC"
     CDS             846..2825
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 200.6; E-value:
                     1.5e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg353_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 7.4e-63, bitscore:
                     200.6, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.6e-37,
                     bitscore: 116.1, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKIDSKIPEGPVAEKWTNYKAHQKLVNPANKRRLDIIVVGTGLA
                     GASAAASLGEMGFRVFNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKG
                     GDYRAREANVYRLAEVSNAIIDQCVAQGVPFAREYGGTLDNRSFGGAQVSRTFYAKGQT
                     GQQLLLGAYSALSRQVNVGTVKLFTRYEMQDVVIIDGRARGIIAKNLITGELERFAAHA
                     VVIATGGYGNAYFLSTNAMGCNCTAAISCYRKGAVFANPAYVQIHPTCIPVHGDKQSKL
                     TLMSESLRNDGRIWVPKKKEDAIKLQKGEIKGSDIPEEDRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSEAINRLGIDVVLQRYGNLFDMYEEITDVNPGE
                     LAKEISGVKYYNPMMIYPAIHYTMGGIWVDYELQTTIKGLFAIGECNFSDHGANRLGAS
                     ALMQGLADGYFVLPYTIQNYLADQITVPRFSTDLPEFAEAEKAVQAKIDKFMSIQGKES
                     VDSIHKKLGHIMWEYVGMGRTAEGLKKGIAELKAVRKEFETNLFIPGSKEGMNVELDKA
                     IRLYDFITMGELVAYDALNRNESCGGHFREEYQTEEGEAKRDDENFFYVACWEYQGDDE
                     KAPVLIKEPLVYEAIKVQTRNYKS"
     CDS             2862..3617
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg353_3"
                     /sec_met_domain="Fer4_7 (E-value: 2.2e-13, bitscore: 38.5,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 1.6e-26, bitscore: 80.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 9.7e-08, bitscore: 20.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKVWRQAGPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQLISERKEPVVFDHDCREGICGMCSLYINGHPHGPATGATTCQIYMRRFNDGDTITVE
                     PWRSAGFPVIKDLMVDRTAYDKIMQAGGYVSVRTGAPQDANAILIPKPIADEAMDAASC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKPEALRRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNISISNIARLNRDFIIAKLKD"
ORIGIN
        1 tctttggtca atcaatctat ttcccctatt tttgcagtct gtaagaggtg taaaacgtcg
       61 aaattttagt tctaacaatt aaataattta aattcaatca tttatgtggt taagtaattc
      121 atctgtagga aggaaagtgg tgatgagcgt taccggtatc gcccttgtcc tgtttctaac
      181 atttcacatg gcgatgaact tagttgcaat catctcggct gatggttaca acatgatttg
      241 tgagtttttg ggagcaaact ggtatgcgct ggtagctacc gcagcactgg ctgctctttt
      301 cattattcac atcatctacg cgttctggct gacaatgcag aatcgcacag cgcgcggtag
      361 cgaacgctat gcagtagtcg acaaaccgaa aactgtagag tgggcttctc aaaacatgtt
      421 ggtgttaggc attatcgtta tcgtaggcct cggtttgcac ctcttcaact tctgggcaaa
      481 aatgcagttg ccggaactga tgcacaacct gggcatgcac gctgatacac tgacgctggc
      541 ttatgcggca aacggtgcat atcacatcca gcagacattc tcttgtccgg tttatgtagt
      601 gctctacctc atttggctgt ttgctctgtg gctccacctg actcacggtt tctggagctc
      661 catgcaatca ctgggatgga acaacaaggt atggatcaat cgttggaaat gtatttctaa
      721 catctattct acgattgtag tactcggttt cgcactcgta gtagtggtat tcttcgtgaa
      781 atcactcctt tgtggcggtg cttgctaaat agtaaattgt aaatgattaa attgtaaata
      841 atattatgat caagatagat tcaaaaattc cagaaggccc ggtggctgag aaatggacca
      901 actataaagc tcaccagaaa ttggtgaacc ccgctaataa acgccgtttg gatatcatcg
      961 ttgtgggtac gggtctggca ggtgcttctg ctgctgcatc cctcggtgaa atgggtttca
     1021 gagtattcaa cttctgtatt caggactctc cgcgccgtgc acactctatc gctgcacagg
     1081 gtggtatcaa tgctgcaaag aattatcaga atgatggtga ctccgtttac cgtctgtttt
     1141 acgatacagt aaaaggtggt gactatcgtg cacgtgaagc caacgtttac cgtctggccg
     1201 aagtgtccaa cgctatcatc gaccaatgcg tggcacaagg tgttcctttc gcacgcgaat
     1261 acggcggtac actggacaac cgttctttcg gtggcgcaca ggtatcacgt acattctacg
     1321 ctaaaggcca gaccggtcag caactcttgc tgggtgctta ctccgcactg agccgtcagg
     1381 taaacgtagg tacggtgaag ttgttcaccc gttatgaaat gcaggacgtt gtgatcatcg
     1441 acggacgtgc acgcggtatc atcgccaaga acttaatcac cggcgaactg gaacgtttcg
     1501 ctgctcacgc cgtagtaatc gctaccggtg gttacggtaa cgcttacttc ctgtctacca
     1561 acgctatggg ttgtaactgt acagctgcta tctcttgcta ccgcaagggt gctgtattcg
     1621 ctaaccccgc atacgttcag attcacccga cttgtatccc ggtacacggt gacaagcagt
     1681 ccaaactgac tctgatgtcc gaatctctgc gtaacgacgg tcgtatctgg gttccgaaga
     1741 agaaagaaga cgctatcaaa ttgcagaaag gcgaaatcaa gggaagcgat attcctgaag
     1801 aagaccgcga ctattatctg gaacgccgtt atccggcatt cggtaacctg gttccacgtg
     1861 acgttgccag ccgtgcagct aaagaacgtt gcgacgcagg tttcggtgtg aacaacacag
     1921 gtctggccgt attcctcgat ttctctgaag ctatcaaccg cctgggcatc gatgtagtgc
     1981 ttcagcgtta tggtaacctc ttcgatatgt atgaagaaat cactgacgtt aatccgggcg
     2041 aactggcaaa agaaatcagt ggtgtgaaat attataatcc gatgatgatt tatccggcta
     2101 tccactacac aatgggtggt atctgggtag actacgaact gcagactacc atcaagggtc
     2161 tgttcgctat cggtgaatgt aacttctccg accacggtgc aaaccgcctc ggtgcttccg
     2221 cattgatgca aggtctggcc gacggttact tcgtattgcc ttacactatc cagaactatc
     2281 tggcagatca gattactgtt cctcgtttct ctactgacct tccggaattt gccgaagcag
     2341 aaaaggcagt tcaggctaag atcgacaagt tcatgagcat ccagggtaaa gaatcagttg
     2401 attctatcca caagaaactg ggtcacatca tgtgggaata cgtaggtatg ggacgtacgg
     2461 cagaaggcct gaagaaaggt atcgccgaac tgaaagcagt ccgcaaggaa ttcgaaacca
     2521 atctgtttat ccccggttct aaggaaggta tgaacgttga gctcgacaag gctatccgtc
     2581 tgtatgactt catcactatg ggcgagctcg ttgcttacga cgcattgaac cgtaacgaaa
     2641 gttgtggtgg tcacttccgt gaagaatacc agactgaaga aggagaagca aaacgtgatg
     2701 acgaaaactt cttctacgta gcttgctggg aatatcaggg tgacgatgaa aaggctcctg
     2761 tattgatcaa agaaccgctg gtatacgaag ctatcaaggt tcagacccgt aactacaaga
     2821 gctaatcggt gaagttaaac atttaaaaga attagacgaa aatggataaa aatatatcat
     2881 ttacactgaa ggtatggcgt caagcaggcc cgaaggctaa aggcgctttt gaaacctacc
     2941 aaatgaaaga tatccccggt gatacttcct tcctcgaaat gctggatatt ctgaacgaac
     3001 agctgatcag cgaaagaaaa gaaccggttg tattcgacca cgactgccgc gaaggtatct
     3061 gcggtatgtg ttctctttac atcaacggac atccgcacgg tcctgcaaca ggtgctacta
     3121 cttgccagat ttacatgcgt cgtttcaacg atggtgacac catcactgtt gagccttggc
     3181 gttcggccgg tttccctgtc atcaaggact tgatggtaga ccgtactgct tatgacaaga
     3241 tcatgcaggc cggtggttat gtaagcgttc gtacaggcgc tccgcaggat gccaacgcta
     3301 tcctgatccc gaagcccatc gctgacgaag ctatggatgc cgcttcttgt atcggttgcg
     3361 gtgcttgtgt agctgcttgt aagaatggtt ctgctatgtt gttcgtttct gcaaaggtca
     3421 gccagctgaa cttgcttccg caaggtaaac cggaagctct gcgccgtgcg aaagctatgt
     3481 tgtctaagat ggatgaactc ggattcggta actgtaccaa cacccgcgct tgcgaggctg
     3541 aatgtccgaa gaacatctct atcagcaaca tcgcccgcct gaaccgcgac ttcatcatcg
     3601 ctaaactgaa agactaagcg tgagcttgat ttgattaata gcataaatcc cccgtcggcg
     3661 ttag
//
