LOCUS       c00228_gnlPro..         5712 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NDGFIKAM_228.
ACCESSION   c00228_gnlPro..
VERSION     c00228_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 09:40:34
            Original ID  :: gnl|Prokka|NDGFIKAM_228
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5712
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5712
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3:4659]"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4..4659
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /neighbourhood="10000"
                     /product="Rnf_complex"
                     /protocluster_number="1"
     cand_cluster    1..5712
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Complex1_51K and minimum(5, [FMN_bind,
                     FeS, Fer4, Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N,
                     SLBB]))"
                     /kind="single"
                     /product="Rnf_complex"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5712
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Rnf_complex"
                     /region_number="1"
                     /rules="(Complex1_51K and minimum(5, [FMN_bind, FeS, Fer4,
                     Fer4_9, NQR2_RnfD_RnfE, Rnf-Nqr, RnfC_N, SLBB]))"
                     /tool="gutsmash"
     CDS             complement(4..588)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Rnf-Nqr"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_1"
                     /sec_met_domain="Rnf-Nqr (E-value: 1.3e-70, bitscore:
                     225.9, seeds: 650, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNFKVMMNGIIKENPTFVLLLGMCPTLGTTSSAINGMGMGLATM
                     FVLICSNVVISLIKNLIPDMVRIPSFIVVIASFVTLLQMVMQAYVPGLYATLGLFIPLI
                     VVNCIVLGRAEAFAAKNNAIASMFDGIGMGLGFTIALTLLGAVREFLGIGKIFDLTIMP
                     EEYGMLVFVLAPGAFIALGYLIALINSFKKA"
     CDS             complement(603..1319)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FMN_bind"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_2"
                     /sec_met_domain="FMN_bind (E-value: 1.6e-23, bitscore:
                     72.2, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLVLTGVTAISVALLAYVNELTKGPIAEANAKTLNEALKKVLPE
                     FTNNPVAESDTIFSEKDGKKNVDFIVYPAKNGEELVGTAVEAKSMGFGGELKVLVGFNA
                     EGKIYNYSLLAHTETPGLGSKADKWFGAYDPAKGEKAVSHEESTKSILGMNPGEAPLTV
                     SKDGGAVDAITASTITSRAFLNAVNAAYQAYKAEGGEVNGVTGASQKAKGADADAADAA
                     TGATIKVELTDSVSAK"
     CDS             complement(1346..2338)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: NQR2_RnfD_RnfE"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_3"
                     /sec_met_domain="NQR2_RnfD_RnfE (E-value: 8.3e-121,
                     bitscore: 391.7, seeds: 472, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENKLIVSLSPHVHGGDSVQKNMYGVLIALIPAFLVSLYFFGLGA
                     LIVTATSVAACLFFEWAIGKYLMKKPTTTICDGSAIITGVLLAFNLPSNLPIWIIILGA
                     LFAIGVGKMSFGGLGCNPFNPALAGRVFLLLSFPVQMTSWPVVGQLTAYTDATTGATPL
                     ALMKQAIYGDTAALSQIPDALTLLIGQNGGCLGEVSALALLIGLVYMLWKKIITWHIPV
                     SILATVFVFAGIMHLADPEKYVSPVLQLLSGGLMLGAVFMATDYVTSPMSKKGMLIYGV
                     CIGLLTVVIRLFGAYPEGMSFAILIMNAFTPLINTYCKPKRFGEVAKKK"
     CDS             complement(2344..3681)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: SLBB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Complex1_51K"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: RnfC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_10"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_4"
                     /sec_met_domain="Fer4_10 (E-value: 6.9e-10, bitscore: 28.0,
                     seeds: 59, tool: rule-based-clusters)"
                     /sec_met_domain="SLBB (E-value: 4.1e-17, bitscore: 50.9,
                     seeds: 67, tool: rule-based-clusters)"
                     /sec_met_domain="Complex1_51K (E-value: 1.2e-51, bitscore:
                     163.5, seeds: 88, tool: rule-based-clusters)"
                     /sec_met_domain="RnfC_N (E-value: 2.6e-30, bitscore: 93.4,
                     seeds: 53, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.4e-06, bitscore: 17.7,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKTFSIGGVHPHENKLSAHQPIITAEVPAKAVILLGQHIGAPAK
                     PVVAKGDVVKVGTKIAEPAGFVSAAIHSSVSGKVAKIDTIVDASGYAKPAIFIDVEGDE
                     WEETIDRSATLVKECELPAEEIVKKIADAGIVGLGGACFPTQVKLCPPPSFKAECVIIN
                     AVECEPYLTADHQLMLEHAEEIMVGVSILMKAVKVNKAFIGIENNKPDAIELMTKVASS
                     YAGIEVVPLKVKYPQGGEKQLIDAITKRQVASGALPISTGAVVQNVGTAFAVYEAVQKN
                     KPLFERVITVTGKSVAKPSNFLARIGTPMKQLIDACGGLPEDTGKVIGGGPMMGKALVN
                     IEVPTAKGSSGILIMNRKEAKRGEAQTCIRCAKCVSACPMGLEPYLLGALSENGDFETM
                     EKERIMDCIECGSCQFTCPANRPLLDYCRLGKGKVGAMIRARQAKK"
     CDS             complement(3706..4659)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: FeS"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Rnf_complex: Fer4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_17"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1016:4Fe-4S dicluster domain (Score: 38.1; E-value:
                     6e-12)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg228_5"
                     /sec_met_domain="Fer4_7 (E-value: 6.8e-13, bitscore: 38.1,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4 (E-value: 1.7e-09, bitscore: 26.3,
                     seeds: 528, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_2 (E-value: 3.5e-09, bitscore: 25.5,
                     seeds: 214, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_17 (E-value: 6.1e-09, bitscore: 25.4,
                     seeds: 82, tool: rule-based-clusters)"
                     /sec_met_domain="FeS (E-value: 1.5e-11, bitscore: 32.9,
                     seeds: 364, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.9e-06, bitscore: 17.3,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLILIAVISLGAIALVLAAILYVASKKFAVYEDPRIAQVGEVLP
                     QANCGGCGYPGCSGFADACVKAGSLDGKFCPVGGQPVMAQIADILGLAAGEAEPMVAVV
                     RCNGTCMNRPRINQYDGAKSCAIAASLYGGETGCSYGCLGCGDCVAACQFDAIHMNPET
                     GLPEVDEAKCTACGACVKACPKAIIEIRPQGKKSRRVYISCVNKDKGAVARKACTVSCI
                     GCGKCVKTCPFEAITLENNLAYIDPHKCKSCRKCVEVCPQNTIIELNFPPRKPKEEAPA
                     APKPAAVSKEAVETPAPAAKVETPAEKATEAPKVTE"
     CDS             complement(4667..5092)
                     /locus_tag="ctg228_6"
                     /transl_table=11
                     /translation="MTNNTIKHLGIVENIQGSHLSVRIVQTSACAACSAKGHCSSADSK
                     DKIIDIIDTAASSYQVGEKVMVVGETSMGMMAVLLAFVLPFVLLIFSLFLLMALVENEL
                     YAALLSLAVLIPYYFVLWLNKTRLKQQFSFTIKPINN"
     CDS             complement(5465..5605)
                     /locus_tag="ctg228_7"
                     /transl_table=11
                     /translation="MKKKTVCCSDLGAYINELLKRAKLKNEYVCETLGMGHDVLNGIKK
                     G"
ORIGIN
        1 gaattaggct ttcttgaaac tgttaatcaa cgcaatgagg tatcccaaag cgatgaaagc
       61 gcccggagcc aatacaaaca ccaacattcc atattcttcc ggcatgatgg tcaaatcgaa
      121 gattttaccg attcccaaaa actcgcggac agcacccagt aaagtcaggg cgatggtaaa
      181 gccaagcccc ataccgatac cgtcgaacat agaagcaatt gcgttattct tggcagcaaa
      241 ggcttcggca cgtcccaaca caatacagtt cactacaatc aacggaatga aaagaccgag
      301 tgtagcatac agtcccggca cgtaagcctg catcaccatt tgaagcaagg tcacgaagga
      361 tgcgatcacc acaataaacg atgggatacg caccatgtcc ggaatcaggt tctttattaa
      421 agagatcacc acattcgagc agatcaatac gaacatggta gccagtccca tacccatacc
      481 attgatagcc gatgatgtag tacccaatgt aggacacata cccagcagga gtacaaacgt
      541 aggattctct ttgataatcc cgttcatcat tactttaaag ttattcatat ctgtctcctt
      601 tcttatttag cactgactga atcagttagt tcaactttga tcgtagctcc cgtagcagcg
      661 tcagcagcat ctgcgtccgc gcctttcgct ttttgagaag caccggttac cccgttcact
      721 tccccgcctt cggccttata ggcttgataa gcagcattca cggcattcaa gaaagcgcgt
      781 gaagtaatgg tagaagctgt gatggcatcc actgctcccc catctttact gacggtcaac
      841 ggagcttcac ccggattcat acccaaaata gatttcgtac tctcttcgtg agatactgcc
      901 ttttctcctt tcgcaggatc atacgctccg aaccatttgt cggctttaga gcccaatccc
      961 ggagtttccg tgtgagccaa cagagagtag ttatagattt ttccttcggc attaaagcct
     1021 accagcactt tcagttcacc gccaaatccc attgatttag cttcaacagc cgtacctaca
     1081 agctcttcac cgttcttagc cggataaacg ataaagtcaa cattcttctt tccatctttc
     1141 tcgctgaaga tcgtatcgct ttccgctacc ggattattgg taaattcggg aagaaccttt
     1201 ttcaaggcct cgttcagtgt cttcgcgttc gcttcggcaa tggggccttt tgtcagttca
     1261 tttacataag ccagcaacgc aacggaaata gcagtcactc ccgtaagtac cagcaacata
     1321 ttctttaaag atgattctaa ctttttcatt tcttcttcgc tacctcccca aagcgttttg
     1381 gtttacaata ggtgttaatc agcggagtga acgcattcat aatcaggatg gcgaacgaca
     1441 tgccttcggg ataggcgccg aacagacgga taactaccgt cagcagaccg atacatacac
     1501 catagatcag catccctttc ttactcatcg gagaagtcac atagtcggtt gccataaaga
     1561 cagcacccag catcagacca ccggaaagca actgcaatac cggagaaacg tatttttccg
     1621 gatctgccag gtgcatgatg cctgcaaaga caaacacagt agccaggata gacacaggaa
     1681 tatgccaggt gatgattttc ttccagagca tataaacgag tccgatcagc agagccagcg
     1741 cactcacttc acccaagcat ccgccattct gtccgatcag caaggtcaat gcgtccggaa
     1801 tctggcttaa agcggcagtg tcaccgtaaa tggcttgctt catcaaggcc agcggagtgg
     1861 caccggtggt agcatccgta taagcggtca actgtccgac taccggccag ctggtcatct
     1921 gcaccgggaa agaaagcagc aggaatacgc ggcctgccaa tgccgggttg aaaggattgc
     1981 agcctaatcc gccgaaagac attttgccta caccgatagc aaacagtgca ccgaggatga
     2041 taatccagat aggcaggtta gacggtaaat taaatgccag caacacacct gtgatgatgg
     2101 cagagccgtc gcagatggtg gtggtcggtt tcttcattaa atattttccg atcgcccatt
     2161 cgaagaacaa acaagccgca acggaagtag ccgtgacaat cagggcaccc agtccgaaga
     2221 aatagagaga caccagaaaa gccggaatca gtgcaatcag cacgccatac atatttttct
     2281 gtacgctgtc tccgccatga acgtggggcg atagtgatac gattaattta ttttccatat
     2341 tcgttatttt ttagcttgac gtgcacgaat catagctccc actttgcctt tacccagacg
     2401 acaatagtcg agcagcggac ggttggcagg acaagtgaac tggcaagagc cgcactcgat
     2461 acaatccatg attctttctt tttccattgt ttcaaaatct ccgttttcag acaaggctcc
     2521 gagcaagtac ggttccagtc ccatcggaca ggcactcacg cacttcgcgc aacggataca
     2581 ggtttgagct tcaccacgtt tggcttcctt ccggttcata atcaggatgc cggaactgcc
     2641 cttcgcagta ggaacctcga tatttaccaa cgccttaccc atcatcggac caccgccgat
     2701 caccttaccc gtatcttcgg gcagaccacc gcaagcgtca atcaactgtt tcatcggtgt
     2761 accgatacga gccaggaagt tggacggctt cgcaaccgac tttcccgtca cggtaatcac
     2821 ccgttcaaac aacggtttat tcttctgcac agcttcataa acggcaaacg ccgttcccac
     2881 gttctgcacg actgctcccg tagagatagg caacgcaccg ctcgccacct gacgtttggt
     2941 gatcgcatcg atcagctgct tttcacctcc ttgcggatat ttcaccttca agggaacgac
     3001 ttcgatgcct gcatagctgg aagctacttt agtcatcagt tcgatagcat cgggcttatt
     3061 attttcgatt ccgataaatg ccttgttcac tttcaccgct ttcatcagga tagaaacgcc
     3121 taccatgatt tcttccgcgt gttccagcat cagctgatgg tcggcagtca aataaggttc
     3181 acattctacg gcattgataa tcacacattc agctttaaaa gaaggaggag gacacagttt
     3241 cacctgtgta gggaaacaag caccacccag accaacgatt ccggcatcgg caattttctt
     3301 cacgatctct tctgccggaa gttcacattc cttcaccagt gtggcgctac ggtcgattgt
     3361 ttcttcccat tcgtcgcctt ccacatcgat aaagatagca ggtttcgcat aaccgctggc
     3421 atcgacaatg gtatcaatct tcgccacttt accactgact gaagagtgaa ttgccgccga
     3481 aacaaagccg gcaggttcag caatcttagt gcctaccttt accacatccc ctttcgctac
     3541 aaccggtttt gcaggtgcac cgatgtgctg ccccagcaaa ataacagcct ttgcaggaac
     3601 ttccgctgtg ataataggtt gatgtgccga caatttattt tcgtgtggat gaactccacc
     3661 aattgaaaat gtctttaaca tacagttctg tattttattc tgttattatt ctgttacttt
     3721 cggagcttct gtagcctttt cagcaggagt ttccactttt gccgccggtg caggagtttc
     3781 caccgcttct ttagaaacag cagccggttt tggagcagcc ggagcttctt ctttcggttt
     3841 cctcggcggg aaattcaatt cgataatggt attctgcgga cagacttcca cacatttgcg
     3901 gcaagatttg cacttgtgcg gatcgatgta agccaagttg ttctccaacg tgatagcttc
     3961 gaacggacaa gtcttcacgc atttgccaca gccgatacag ctcactgtac aagccttacg
     4021 tgccacagct cccttgtctt tattcacaca agagatatac acacggcgtg atttctttcc
     4081 ttgcggacgg atttcgatga tcgccttcgg acaagccttc acacaggcac cgcaagcggt
     4141 acatttcgct tcatccactt ccggaagtcc tgtttcagga ttcatgtgga tggcatcgaa
     4201 ctggcaggca gccacacaat caccacaccc cagacatccg taactacaac ccgtttcccc
     4261 accatagagc gaagcggcaa tagcgcaact cttcgcaccg tcgtactgat taatacgcgg
     4321 acgattcata cacgttccgt tacatcttac cactgctacc atcggttcag cttctccggc
     4381 tgcaagccct aggatatcag caatttgtgc catgacaggc tgtccgccta cggggcagaa
     4441 tttaccatcc agcgaaccgg ctttgacgca agcgtcggca aagccgctac aaccgggata
     4501 gccacatcca ccacaattag cctggggaag cacttctccc acctgggcaa tccgcgggtc
     4561 ttcatacacg gcaaatttct tggaagccac ataaagaata gcggccaaca cgagcgctat
     4621 agctcccaat gaaatcactg caatcagaat caaattcata atgtttttag ttatttattg
     4681 gttttatagt aaatgaaaat tgttgtttga gtcgcgtttt gttaagccat aaaacgaaat
     4741 aataaggtat aaggacagcc agagaaagaa gagccgcata cagttcattc tctaccaatg
     4801 ccatcaaaag aaacaacgaa aatataagaa gtacgaaagg aagtacgaaa gccaacaata
     4861 ccgccatcat gcccatcgac gtttcgccga ccaccatcac cttctctccc acctgatagg
     4921 aagaagctgc ggtatctatg atatctatga ttttgtcctt actatccgcc gaggaacaat
     4981 gcccctttgc gctacatgcc gcacaagccg atgtctggac aatcctcacc gacagatgag
     5041 aaccctgtat gttttccaca atacctagat gctttatagt attattcgtc attttgcaaa
     5101 ctccgcatta caaatcgcgg caaatttaat cattatttgt gaactacgaa gcattcctca
     5161 aactttttct acctaataaa atgttatttt ttgtgttttc gttatcgcac acggaaaagg
     5221 tatggctacc atttgatatt tcccaagaat gacaaataga catcctaaac aaaaaaatcc
     5281 cggccgtttt tatttattca gccgggattt tctatcaatt atttcacgga ttactccact
     5341 atcctcttct tgtagtagcg cagatacatt ctcaccaacg tattcaaaat cagttcaatc
     5401 tcctcttcgt tgtaggcatt caaacagaga ttcattactt ttatataatc gttcaatggg
     5461 agatttagcc cttttttatg ccattcaaaa catcatgtcc catacccagg gtctcgcata
     5521 catactcatt tttcaatttc gcacgtttaa gcaactcatt aatataagct cccaaatcac
     5581 tgcaacaaac cgttttcttt ttcatctttg ttttttgttg ttaaatacac actttaataa
     5641 ttaaatattt taacttttat cctcatatat cagggtagag tctattttgt tattactacc
     5701 ttcatgccgt gt
//
