LOCUS       c00315_gnlPro..         4544 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NDGFIKAM_315.
ACCESSION   c00315_gnlPro..
VERSION     c00315_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 09:40:34
            Original ID  :: gnl|Prokka|NDGFIKAM_315
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4544
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4544
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:3907](-)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      complement(2..3907)
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..4544
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4544
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(2..3907)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg315_1"
                     /sec_met_domain="HGD-D (E-value: 3.6e-24, bitscore: 72.1,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 1.5e-35, bitscore:
                     109.0, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNAYKVGLDIGSTTTKMVVLDKDGTTVLFSGYKRHQAKIQECLLG
                     FLYQIKEQLGDIPLSFHITGSVGMGISEKCSLPFVQEVVAATNYVRREHPHTATMIDIG
                     GEDAKVVFFQDGQATDLRMNGNCAGGTGAFIDQMAILLGVSIDELNELALRSNQVYSIA
                     SRCGVFCKTDIQNLIAKNISKENIAASIFHAVAVQTTVTLARGCNIVAPVLLCGGPLTF
                     IPALRKAFANYLNLSQDTDFILPEKGNLIPAWGAALAENNEESIHLSNLIQVIENKLSV
                     TSPFQSDLSPIFDSEEEYRSWKKEKDQYKIQYASLRPGLQEATIGIDSGSTTTKIVVLD
                     NNHRILYSYYHDNNGNPIKTVENGLQKLYEECRRRGTTLRIKGGCSTGYGEDLIKAAFH
                     MDAGIIETIAHYAAAKHISKEVSFILDIGGQDMKAIFVNDGVINRIEINEACSSGCGSF
                     ISTFAQSLDYSVEDFAKAACFSQAPCDLGTRCTVFMNSKVKQVLREGASVADIAAGLSY
                     SVVKNCLYKVLQLKDTEILGDHIVVQGGTMRNDSIVRSLEKLTGKQTFRSNRPELMGAL
                     GCALYAKQIENARVTELNEMLQWAQYTSKQLQCRGCENQCAIMRYTFNSENHYFSGNRC
                     EKVFSNKGSHADKGINTYDKKLELLFDRSADIPQPLFTIGIPRILNMYEEYPFWHTLFT
                     ACGIQVQLSEPSTFSKYETAAGMVMSDNICFPAKLVHSHIRNLTQQNVNRIFMPFVVFE
                     KKDKQQQNSYNCPIVSGYSEVIKSVQEENIPIDAPTITFKDEALLYKQCYEYLKSLGIR
                     DEVCKNAFSRALQEQYAFEEKIAAYNQEVLNEGREKHKLIILLAGRPYHSDPLIQHKVS
                     DMIAAMGVYVITDDIVRQQEISLEKTHYLSQWAFTNRILKATKWAAMQEGDIQYMQMTS
                     FGCGPDAFLIDEVRNLLKRYGKNLTLLKIDDVNNIGSIKLRVRSLVESLNFSLKHSQAK
                     DPEPFVSTAPFTKKDKKKKILAPFFTPFISPLIPSIMKVAGYEMETLPLSDTASCDWGL
                     KYSNNEVCYPATLIVGDIVKAFKSGRYDPANTCVAITQTGGQCRASNYISLIKKALIEN
                     GYTNTPVVSLAFGSGIENEQSGFKVNWLKVLPIILASVLYSDCIAKFYYAAVVREKERG
                     QAARLRDLYLDTAQPIIQKNKPEDLLSYLYLAARDFNKICEQRSCHKVGIVGEIFLKFN
                     PFAQKDVTSWLINQKIEVIPPLISDFFMQGFVNLKVRQNQHLQRKLTPDFVIDWLYKKV
                     QKQINKVNEIGKSFNYFI"
ORIGIN
        1 gtatgaagta attgaaactt ttgccgattt cattcacctt attaatttgt ttctggactt
       61 tcttgtataa ccagtctata acaaagtcgg gagtgagttt tcgctgcaag tgttggttct
      121 gtctcacttt caggttgaca aatccctgca tgaagaaatc acttatcagt ggcggaatca
      181 cttcgatctt ctggttgatg agccatgagg tgacgtcctt ttgagcaaaa gggttgaact
      241 tcaggaatat ttccccaaca atacctactt tgtgacagga tctctgttca cagattttgt
      301 tgaagtctct ggcagcgaga tataagtagc tcaacaagtc ctccggttta tttttctgga
      361 taatgggctg tgctgtatcc agataaagat cgcgcagacg ggcggcctgt ccccgttctt
      421 tctcacgaac gacagctgca taatagaatt tggcaatgca atcactgtat aacacagacg
      481 caagtatgat tggcaaaacc ttgagccagt ttactttaaa accggattgc tcattttcaa
      541 taccactgcc gaaagcaagt gataccaccg gtgtattggt gtatccattc tcaatcaatg
      601 ccttttttat cagtgaaata tagttgctgg cacgacattg tccaccggtt tgtgtgatgg
      661 caacgcacgt attggcagga tcataacgtc cgcttttaaa ggcttttact atgtcgccga
      721 caatcaatgt tgccggataa catacctcat tattggagta tttcaatccc cagtcgcaag
      781 atgcggtgtc gctcaacggt aacgtttcca tttcatatcc ggccactttc atgatggatg
      841 gaatgagtgg tgaaatgaaa ggcgtaaaga atggagcgag gattttcttc tttttgtctt
      901 tcttggtgaa aggagcagtt gaaacaaatg gctcgggatc tttcgcttga gaatgtttca
      961 aactgaaatt caagctttcc accaacgagc ggactcgtaa tttgatggag ccgatattat
     1021 taacatcgtc tatcttgagc aaagtaagat tcttgccgta tcgtttcagc aagttacgta
     1081 cttcatctat aaggaaagca tccggaccgc agccaaaaga agtcatctgc atatattgta
     1141 tgtcaccctc ttgcattgcc gcccacttgg ttgctttcag aatacggttg gtaaatgccc
     1201 attgagagag gtaatgagtc ttttccagtg aaatctcctg ctggcggaca atatcatcag
     1261 ttatcacata aactcccatc gcagcaatca tatccgaaac cttgtgctgt atcagtggat
     1321 cgctatggta agggcgtccg gcgagtaaga taatcagttt gtgtttttcc cgtccctcgt
     1381 tcagaacttc ctgattatag gcagcaattt tttcttcgaa agcatattgt tcctgtaatg
     1441 cgcgtgagaa ggcatttttg catacctcat ccctgatacc caaagatttc agatattcat
     1501 agcattgttt atacagcaac gcttcatctt tgaaggtgat agtcggagca tctatcggta
     1561 tattctcttc ctgtacactt ttgatgactt ccgaataacc ggaaacgatg gggcaattat
     1621 agctgttttg ctgttgctta tcctttttct cgaaaacgac gaacggcata aatatccggt
     1681 tgacgttttg ttgcgtcaga ttacggatat gactgtgcac aagtttggca gggaagcaga
     1741 tgttatcaga catcaccatg ccggcagcag tctcgtattt gctgaatgta gagggttccg
     1801 acaactgtac ctgaatgccg caagcagtaa ataaggtatg ccagaaaggg tattcctcat
     1861 acatgttcag aatacgtgga atacctatgg tgaatagggg ttgtggaatg tctgcgctcc
     1921 ggtcgaataa taattccagc tttttatcat acgtattgat gcctttgtcc gcatggcttc
     1981 ctttattgga aaacaccttc tcacaccggt tgccggagaa gtaatggttt tcactgttga
     2041 atgtatatcg cataatggcg cactggttct cgcacccccg gcattgaagt tgcttggaag
     2101 tatattgcgc ccattggagc atctcgttca gctccgtcac ccgggcattt tcgatttgct
     2161 tggcatatag ggcacaacca agagcaccca tcaattcagg acggttactg cggaaggttt
     2221 gtttcccggt cagtttttcc agacttcgta cgatggaatc attacgcatg gttcctccct
     2281 gcacgacaat atggtcgcca agtatctccg tgtcttttaa ttgcagaacc ttatacaggc
     2341 agtttttaac aacggaataa gataatccgg cagctatatc cgctacggac gctccttccc
     2401 gaagtacctg ttttactttg gagttcatga agactgtgca acgtgtaccc agatcacatg
     2461 gtgcttgcga aaaacaagct gctttggcaa agtcctccac actgtaatcc agcgattgcg
     2521 caaatgtgct gataaaagaa ccgcaaccgg aggaacaggc ttcattgatt tcgattctgt
     2581 tgattactcc gtcgttcaca aagatggctt tcatatcctg ccctccaata tccaggataa
     2641 aagaaacttc tttgctgata tgtttggcag cagcataatg agcgatggtt tcaatgattc
     2701 ccgcatccat gtggaaagcc gcttttatca gatcttctcc gtaaccggta gaacatcctc
     2761 cttttatgcg cagggtagtt cctcgtctcc ggcattcttc atatagtttc tgcaagccgt
     2821 tctctactgt ttttataggg tttccgttgt tgtcgtgata ataggagtaa aggatacgat
     2881 gattgttgtc gagtactacg attttggtgg tagtagagcc ggagtctatg ccgatagttg
     2941 cttcctgtaa tccgggacgc agactggcat attgaatttt atactggtct ttttcttttt
     3001 tccatgaccg atattcttcc tcactgtcga atataggaga aagatcactc tggaaaggtg
     3061 aggtaacact tagtttgttt tctattacct gtatcaggtt ggagagatga atactttctt
     3121 cattgttctc tgccaatgct gctccccagg cagggatcag atttcctttt tccggtaata
     3181 tgaaatctgt atcttgtgac agattcagat agttggcaaa tgctttccga agggccggaa
     3241 taaaagtcaa tgggccaccg cagagcaata ccggagctac gatattgcaa ccgcgtgcaa
     3301 gtgtgacagt ggtctgtacg gctactgcat ggaaaatgga agctgctata ttttctttgg
     3361 atatattttt ggctatcaga ttctgtatgt ccgttttgca gaatactccg cagcgtgaag
     3421 cgatagagta tacctgattg gaacgaagtg ctagttcatt caattcgtcg atggagactc
     3481 caagcagaat agccatctgg tcaatgaaag ccccggttcc gccggcgcaa tttccattca
     3541 tgcgcaggtc tgtagcctgt ccgtcctgaa agaaaacaac tttcgcatct tctcctccaa
     3601 tatcgatcat agtggctgtg tgcggatgct cccggcgcac atagtttgtt gcagctacaa
     3661 cctcttgcac aaacggcaga gagcatttct ctgaaattcc cattccaaca gagcccgtaa
     3721 tatggaaaga taaaggaata tcacccaact gttcctttat ttgataaaga aatccgagta
     3781 aacattcttg tatttttgcc tgatgccttt tgtatccgga aaacaagact gtagttccat
     3841 ctttgtccaa tactaccatc ttagtggtag tagagcctat atctaaaccc actttatatg
     3901 cattcatata ccaaacatct taaattagac ctcgtgtttg acacttgtgt cagacgcaaa
     3961 tgtatgtaaa aatatggtag atatggcaaa tcgtaattga agttttgggt ttaattaacg
     4021 aattgggaaa aagaaagatg gaatgtgagg gaggaaaacg atcgtgtaaa aaagaagtaa
     4081 atgaataatt gtgtatttct attacttgtt aggttgtagt agatctttta tggtgaatct
     4141 tagaaatctt gtttactcta tttttgacaa aaggtaacgt ggcttttaat aaacccggat
     4201 tctgtttata ataaactttt tgatagtttt agtaaaacgg tattcgagtt tattataaac
     4261 tattgtagaa cagttttagg atcagacgag agattttaag ctgaattata aacaaaatgg
     4321 ggcaataatc attcgactat tgccccatat cgtataggag tggtattgta aaataccagg
     4381 ttatttatga tttatgtatg attatttctt tctattttca tgttgtttat actttttctt
     4441 ttctcaaagc tacaaaataa aaagtcattt gtcaatagtc tgatgagaaa agtagctaat
     4501 cggtctcaaa acagatcaat atctttactt ctaatatctg attt
//
