LOCUS       c00316_gnlPro..         4524 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NDGFIKAM_316.
ACCESSION   c00316_gnlPro..
VERSION     c00316_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 09:40:34
            Original ID  :: gnl|Prokka|NDGFIKAM_316
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4524
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4524
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[283:3256]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      284..3256
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4524
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4524
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             284..925
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg316_1"
                     /sec_met_domain="PFL_acetate1 (E-value: 9.6e-111, bitscore:
                     359.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAE"
     CDS             953..2512
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg316_2"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-47, bitscore:
                     149.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 8.1e-237, bitscore:
                     775.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVS
                     SFLDIYMEYELSKGTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLG
                     GRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQ
                     YENDDLMREVRQSDDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTV
                     MVKNIPVLTSDTLNFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDT
                     NPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDH
                     LGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGAN
                     PMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYF
                     TKGAHHLNVNVLNREMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHE
                     RM"
     CDS             2528..3256
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg316_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.3e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3284..3727)
                     /locus_tag="ctg316_4"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTTHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDEDGKAADTRTSRQKESLHRLVRELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(3714..3905)
                     /locus_tag="ctg316_5"
                     /transl_table=11
                     /translation="MITLILDQMKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATH
                     KEVCNVKTTEREKDDEKD"
     CDS             complement(3902..4405)
                     /locus_tag="ctg316_6"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLKIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
ORIGIN
        1 cggttttcaa gaccgccgca atcgaccact ctgccacctc tccaaaactt ctttatcaga
       61 agtgcttttc gttaaaagcg gtgcaaaggt acgaatatat tttaaactta caagagtttt
      121 cacaaaaaaa atcacgaaca tcatttccaa agaatagcaa tatctaggta ttttatgttg
      181 aaaagcataa aatagagcat atttatgctg attatatgaa aaaacgttgt aactttgtgc
      241 gctctaaaga aaaggataac tacaacctct aattttatca tttatggaat taaataagat
      301 ctttaaagac ggtctttgga gcacagaaat caacgtaaga gatttcgtta gtcacaacat
      361 cactccgtat tatggagatg cttctttcct cgaaggacct accgaacgca caaaagctgt
      421 atggaaccgc tgcctcgaag cactggcaga agaaagagcc aataacggcg tacgctcact
      481 ggataatgtc accgtctcaa ccatcacttc tcataaagcc ggatatatcg acaaagaaaa
      541 cgaactgatc gtcggtctgc agacagacga acttctgaaa cgtgcaatca aacctttcgg
      601 aggtatcaac gtagtgagca aagcctgcca cgaaaacggc gtggaagtgg acgaccgcgt
      661 aaaagatatc ttcactcact accgcaagac gcacaacgac ggagtatttg acgtatatac
      721 cgaagaaatc cgttcgttcc gttctctggg attcctcacc ggacttcctg acaactatgc
      781 ccgcggacgt atcatcggcg actaccgccg tatggctctc tatggtatcg accgactgat
      841 tgaggcaaaa aaagaagatt tgcgtaacct taccggtccg atgaccgaag cccgcatccg
      901 cctgcgcgaa gaagtggcag agtaaatcaa agcattaaaa gacatgaagg taatgggcga
      961 atactacgga ctcgacttga gccgtcctgc ctacaccgca caagaagcag tacaatgggt
     1021 atacatggct taccttgctg ccgtaaaaga acaagacggt gctgctatgt cactaggtaa
     1081 cgtctcttcg ttcctcgaca tttatatgga atatgaattg agcaaaggaa ccatcacaga
     1141 atctttcgca caggagctga tcgaccagtt tgtcatcaaa ctgcgtatgg tgcgccacct
     1201 gcgtatgcaa tcttacaacg acatctttgc cggcgacccg acatgggtaa ccgaatctct
     1261 gggcggacgc ctcaacgacg gacgcacgaa ggtgacaaag acttctttcc gcttcctgca
     1321 aacactctac aacctcggcc cttcaccgga accgaacctg actgtattgt ggagcccgga
     1381 acttccggaa ggattcaaag aattctgcgc aaaagtatct atcgacactt cttccatcca
     1441 atatgaaaac gacgacctga tgcgtgaagt acgccagtct gacgactacg gaatcgcgtg
     1501 ctgcgtatct tatcaggaaa tcggaaaaca aatccaattc ttcggcgcac gttgcaacct
     1561 ggcaaaagcc ctgttgcttg ccatcaacgg cggacgttgc gaaaacaccg gtacggtaat
     1621 ggtgaaaaat atccccgtac taaccagcga cacactgaac ttcgaagaag taatgagcaa
     1681 ctacaagaag gtactgacag aaatcgcccg tgtttacaac gaagcgatga acatcatcca
     1741 ttatatgcat gataagtatt actacgaaaa agcccaaatg gctcttgtag ataccaaccc
     1801 acgcatcaac cttgcttatg gtgtagccgg actttctatc gccctcgatt cactgtcggc
     1861 catcaaatat gccaaagtga ccgcacgccg caacgacata ggtctgacag aaggtttcga
     1921 tattcaaggc gaattccctt gcttcggtaa tgacaacgac aaggtagacc acctgggtgt
     1981 tgacctggtc tatttcttca gcgaagaatt gaagaaactt ccggtttaca aaaatgcccg
     2041 tcctacttta tctttgctca ccatcacttc caacgtgatg tatggcaaga agacaggtgc
     2101 tactcccgac ggacgcgcca aaggtgtcgc ttttgctccg ggagccaacc ctatgcacgg
     2161 acgtgacaag aacggtgcca tcgcctcttt gagttccgta gcgaaattac gttaccgcga
     2221 ctcacaggac ggtatcagca acactttctc catcgtcccc aaatcactgg gagctactga
     2281 agaggatcgc gtagaaaatc tggtgacaat gatggatggc tatttcacca aaggcgctca
     2341 ccacctgaac gtgaacgtac tgaatcgtga aatgctttac gatgccatgg aacatccgga
     2401 aaaatatccg caactcacca tccgtgtttc cggttatgcc gtaaacttcg tgaagttgag
     2461 ccgcgaacat caattggaag taatcagccg tagcttccac gaacgtatgt aatcagatct
     2521 actcaatatg acgataaacg tacattcata cgaaagtatg ggaacattcg acgggccggg
     2581 cttacggctc gtcgtttttc ttcaaggatg caatttccgc tgcctgtatt gcgccaaccc
     2641 tgacacaata gccgggaaag gaggcacacc tactccaccg gaagaaatcg tccgcatggc
     2701 tatgagccaa cgtccttttt tcgggaaacg cggaggagtc actttctccg gaggagaacc
     2761 tacgtttcag gccaaagcac tagtcccttt agtgcgtgaa ctgaaagaaa agggaataca
     2821 tgtatgcata gacagcaacg gcggtatctg gaacgaagaa gtagaagaac ttttcaaact
     2881 gacggatctt gtattgctgg atattaaaga gttcaatccg gcacgccatc aagcactgac
     2941 cggaagaagt aacgagcaaa ccatccgcac ggcagcatgg ctggaagaaa acgagaaacc
     3001 tttctggctg cgttatgtat tagtgcccgg atacagcgat ttcgaggaag acatccgccg
     3061 tttaggagaa gctctgggaa aatataagat ggtgcaacga gtggaaatcc tgccttatca
     3121 tacattaggc gtacataagt atgaagcgat ggagcaggaa tacaagctga aagacgtaaa
     3181 agaaaatacc cccgaacaac ttgaaaaagc cgcggaggta tttaaagagt atttcactac
     3241 tgtggtggtc aattaaccac cacagtaata aataggataa aaatcaccat gtctctgctt
     3301 tcacctcgaa acagggacac tctttgatcc gctcccaagg gtcaacaatt ccattataat
     3361 tggtgtcagg actcaagtcc cgatgaccga ccacctttgc atccggataa cgctgcaaca
     3421 gttcgcgcac caggcgatgc aaagattctt tctggcggga agtacgcgta tccgctgcct
     3481 ttccgtcctc atccagtccg ccttcatagc acacccctaa actaatcgca ttaaaaccgc
     3541 gtgcatgagc acccaccgta tcttcatccc tcatcggctc tatctgtccc gacttgcgaa
     3601 tgtagtaatg atacccccaa cacgaaaagc cccgaaagcg atgggtagtg tccacatcct
     3661 tcgccgtgaa gtcgcgatca gcacgggtag cagagcagtg aatcacaatc agactaatct
     3721 ttctcatcgt ccttctccct ttccgttgtt ttcacattac acacttcctt atgggtagca
     3781 ttgaagcgca cctctctcac aattctgttc cgctttcttt ttccgaagaa aggcacaagg
     3841 aacatgatta catccaaaat cttagttaca atttctttca tttgatccaa tattaaagtt
     3901 atcatgctgc cgggtccgga gcttctccgc ttccaccacc gtctgaacca ccaccggaac
     3961 ctttgtcatc gtcttctcct tcattgccgc taccgcccgt ttgagccacc gcacgcgtcg
     4021 ttacctgctg gtagctaaga atcttcgtga tgtccgccaa cgtctttccg ggacgaaaaa
     4081 taatatgggc agccgtaatc agcgaacttg aaaactcctt gaccgacaca gctccttcac
     4141 tgttcaaccc gatcttcaaa ctgcccaaat cacccaaccg gactattctt cccgctttca
     4201 acgcacgctt cgcctcataa agaatacccg aaatggcggc acgcacatct gtctccgtac
     4261 aggtagaccg actggtgatt ccttcgcaca actcttcaaa atccaactct cccgaagcct
     4321 gtgcctgcgc gtaatacttt gccgactcat cctttttggc aggattcttg cgcggcacta
     4381 ctgaataagt tactgtcatt gccataataa aataatttta agatttcaca catttcgaac
     4441 gcgttcctta tttgttaacg acataaagat aacacatttt ccatcctccg gattccccaa
     4501 cgaagtttcc tgatactcta tgaa
//
