LOCUS       c00351_gnlPro..         4048 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NDGFIKAM_351.
ACCESSION   c00351_gnlPro..
VERSION     c00351_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 09:40:34
            Original ID  :: gnl|Prokka|NDGFIKAM_351
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4048
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4048
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[56:4046]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      57..4046
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4048
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4048
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             57..461
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg351_1"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-24, bitscore:
                     75.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGETKIELLEPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAEGKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
     CDS             490..2043
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg351_2"
                     /sec_met_domain="CT_Btheta (E-value: 8.1e-291, bitscore:
                     952.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERLAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYVFAQDFTVSAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAKTEEEGFELIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPT
                     KPYDMYEVIGAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             2075..2995
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg351_3"
                     /sec_met_domain="OAD_gamma (E-value: 2.8e-16, bitscore:
                     48.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLVLNEILIDNQNNFQD
                     DYGLHSAWIEIFNKSFGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWIGLFDSGRKLLDKIVVPAGVLGPNQSYARVSDGAAEW
                     EVKGGSGDKYVTPSTNNKTLDSNAKMEKFEEHDADGVGMSISAMSVVFCGLILLFIAFK
                     IVGKVAVNLSKRNAMKSKGIDKHEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRETPPRK"
     CDS             3020..3451
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.7; E-value:
                     2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg351_4"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.6e-22, bitscore:
                     67.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKPA
                     AKPVTVRPMPNAPTAPTQVVKPTAPSTGKSGVKSPLPGVILDIKVNVGDTVKKGQTIII
                     LEAMKMENNINADKDGKITAINVNKGDSVLEGNDLVIIE"
     CDS             3453..4046
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg351_5"
                     /sec_met_domain="OAD_beta (E-value: 1.8e-71, bitscore:
                     229.3, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMILVGLGFIYLAVAK
                     EFEPMLLIPIGFGMLIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAESAYSYMALVPVIQPPIMRLLT"
ORIGIN
        1 gtgctgtttt atgccttata agcacagatt taaaaccaaa taaaaaaagt atagttatga
       61 agatttcaca cattgaacat ctgggcattg ctgtaaaaag catcgaagaa gcccttcctt
      121 actacgaaaa tgtattaggt ctgaagtgtt acaacattga aacagtggaa gatcagaaag
      181 taagaactgc ttttttaaaa gtgggcgaaa caaaaattga actgttggag cctacttgcc
      241 cggagagtac tattgccaaa ttcattgaaa acaaaggtgc aggcgttcac cacgtcgcat
      301 ttgctgtaga agatggcgta gccaatgcgc tggcagaggc agaaggcaaa gaaatccgcc
      361 ttatcgacaa agctccccgc aaaggtgctg aaggtttgaa tatcgctttc cttcacccga
      421 aatcaacttt gggtgtgtta acagaactct gcgaacatta atcataaact ctaaaaccaa
      481 caagaactca tgagtaatca acttgaaaaa attaaagagc ttattgaacg ccgtgccgta
      541 gcacgtatcg gaggcggtga aaaagcaatt gcgaagcaac acgaaaaagg gaaatacaca
      601 gcacgcgagc gtctggctat gctgcttgac gagggtagct tcgaagaaat ggacatgttc
      661 gttgagcaca gatgcacgaa cttcggcatg gagaaaaaac attacccggg agacggtgta
      721 gtaaccggct gcggtacgat cgaaggacgt ctggtatatg tgttcgcaca ggacttcact
      781 gtttctgccg gttcactgtc agaaacgatg tcattgaaaa tttgtaaaat catggatcaa
      841 gccatgaaga tgggcgctcc ctgtatcggt atcaacgact cgggtggtgc acgtattcag
      901 gaaggtatca acgctttggc aggttatgca gaaatcttcc aacgcaatat ccttgcttcc
      961 ggtgttatcc cacagatttc aggtatcttc ggtccttgtg ccggcggtgc tgtttattct
     1021 ccggctttga ccgacttcac gctaatgatg gaaggtactt cttatatgtt ccttaccgga
     1081 ccaaaagttg tgaagaccgt aacaggtgaa gacgttagcc aggaaaacct cggtggcgca
     1141 agcgttcact ccactaaatc aggtgtgact catttcaccg ctaaaacaga agaagaaggt
     1201 ttcgaactga ttcgtaaact gttgagttat attcctcaaa acaaccttga agaagctcct
     1261 tatgtagatt gtacagaccc aatcgaccgt ttggaagatt ctttgaatga tattatcccc
     1321 gacagcccta ctaaaccgta tgacatgtac gaagttatcg gcgctattgt ggacaacggt
     1381 gagttccttg aaatccagaa agattatgca aagaatatca tcattggttt cgcccgtttc
     1441 aacggacaat cggtaggtat tgttgccaat cagcctaagt atctggctgg tgtgctcgac
     1501 agcaacgcat ctcgcaaggg tgcacgtttc gtgcgtttct gcgatgcatt caatattcct
     1561 atcgtatcgt tagtagacgt accgggattc cttccgggaa caggtcagga atacaacggc
     1621 gttatccttc atggtgctaa gctattgtat gcttacggtg aagctactgt acctaaggtt
     1681 acgattacat tgcgtaaatc ctacggtggt tctcacatcg taatgagctg taagcaactt
     1741 cgcggtgaca tgaactacgc atggccgact gctgaaatcg cagtaatggg tggagcagga
     1801 gcagtagaag tattgtacgc acgcgaagcc aaagatcagg aaaatcccgc tcaattcctt
     1861 gctgaaaaag aagcagaata cacgaaactg tttgccaatc cttacaatgc agccaaatac
     1921 ggctacatcg acgatgtgat tgaaccacgc aacacgcgtt tccgcgtaat ccgtgctttg
     1981 cagcagttgc agactaagaa attgagtaat ccggctaaga agcatggtaa tattccgttg
     2041 taatcctact tttcacatta aaacaagaac gattatgaac aaaaccaaaa tcggaatatt
     2101 cctttctttg ctgttgctga ttgggctgac ttcttgtgga gagcaaaagt cgaacaacaa
     2161 gctggtactg aacgaaatcc tgatagacaa tcagaataat tttcaagatg actacggatt
     2221 gcacagcgca tggattgaaa tattcaataa atcattcggt agcgccgacc tggcagcttg
     2281 cttgttgaaa gtaagcagcc aaccgggcga tacagttact tattttatcc cgaaaggtga
     2341 tatacttaca ttagtgaagc cacgtcaaca tgcactattc tgggcagatg gcgaacctaa
     2401 ccgcggtact ttccacacca gcttcaagct gaatccggaa acggccaact ggatcggtct
     2461 gtttgactcc ggtagaaagc tgctcgacaa gattgtagtg cccgcaggtg ttcttggtcc
     2521 caaccagtca tacgctcgtg tcagcgacgg agcagcggag tgggaggtaa aaggtggaag
     2581 tggtgacaaa tacgtgaccc cgagtacgaa caacaaaact cttgacagca atgccaagat
     2641 ggaaaaattc gaagagcatg atgctgacgg tgtcggcatg tctatctctg ccatgagtgt
     2701 agtattctgc ggattgattc ttctctttat tgccttcaag attgtaggaa aagtagctgt
     2761 aaatttgagc aagcgcaatg ctatgaaatc taaaggcatt gacaagcatg aagctaagga
     2821 actgtcacag gctccgggcg aagtttacgc tgcgatttct atggcattgc acgaaatgca
     2881 ggatgaagtg cacgacgtag aagaaacggt actgaccatc actcgcgtaa aacgcagtta
     2941 ctcaccatgg agttcgaaga tttacacttt gcgtgaaact cctcccagaa agtaagtcac
     3001 cttttaaaaa gttaaaacga tgaaagaata taaatataaa atcaacggta actcatataa
     3061 agtaaccatc ggagatattg aagataatat cgctcatgta gaagtgaacg gtacacacta
     3121 taaagtggaa atggagaaac agccgaagcc tgctgccaaa cctgtaacag tacgtcctat
     3181 gcctaatgct cctactgctc ctactcaggt agtgaaaccg actgctccgt ctaccggaaa
     3241 atcaggagtg aaatctccgc tgccgggtgt tatcctcgac atcaaagtga atgtaggcga
     3301 tactgtaaag aaaggacaga ccattatcat attggaagcc atgaagatgg aaaacaatat
     3361 caatgcggat aaggacggta agattactgc catcaacgtc aacaagggag actctgttct
     3421 tgaaggtaat gacctcgtaa ttattgaata atatgggaga ttttataaac tttttaggaa
     3481 ataacctcgc cgacttctgg acatacacgg gctttgcaaa tgctactgta gggcatgtcg
     3541 ttatgattct tgtgggtttg gggttcatct atttggcggt agccaaagag ttcgaaccga
     3601 tgctgctgat tcctatcggc ttcggtatgc tgatcggtaa tatacctttt aatatggatg
     3661 ccggactgaa agtcggtatt tatgaagaag gttccgtatt gaatatattg tatcagggag
     3721 taacttccgg ctggtatccg ccgctcatct ttttgggcat cggcgccatg acggacttct
     3781 cggcacttat ctctaatccg aaattgatgt tgatcggtgc tgctgcacaa ttcggtatct
     3841 tcggtgctta catgatcgca ttggaaatgg gcttcgaccc gatgcaagcc ggcgctatcg
     3901 gtatcattgg tggagcagac ggcccgacgg ccattttcct ttcttccaag ctagcaccta
     3961 acctgatggg agccattgcg gagtccgcct actcctatat ggcgttggtt ccggtgatac
     4021 agccgcctat catgcggctg ctcaccac
//
