LOCUS       c00062_gnlPro..         3400 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HNAKIJJP_85.
ACCESSION   c00062_gnlPro..
VERSION     c00062_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:00:27
            Original ID  :: gnl|Prokka|HNAKIJJP_85
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3400
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3400
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[379:3350]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      380..3350
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3400
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3400
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             380..2608
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg62_1"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTDARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLIEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2622..3350
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.7;
                     E-value: 6.1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg62_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-31, bitscore:
                     97.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
ORIGIN
        1 tgggtgtaaa attgggtgtt tgttttataa tttgctgatt ttcagcgttt attgcggaga
       61 gacagggatt cgaaccccgg gtacctcgcg gtacaacggt tttcaagacc gccgcaatcg
      121 accactctgc cacctctcca aaacttcctt atcagaagtg cttttcgtta aaagcggtgc
      181 aaaggtacga agatatttta aacttgcaag agttttcact aaaaatttaa tctaatcttt
      241 tcctaaggat agcaattaat aggtatttta tgttgaaaag cataaaatag agcatattta
      301 tgctgattat atgaaaaatg attgtaactt tgtgcgctct aaagaatagg ataactacaa
      361 cctctaattt tatcatttta tggaattaaa caagatcttt aaagacggtc tttggagcag
      421 cgaaatcaac gtcagagatt tcgtaagtca taacatcact ccgtattacg gagatgcttc
      481 attcctcgaa ggacctacag aacgtaccaa agccgtatgg aaccgctgcc tcgaagcgtt
      541 ggcagaagaa agagaaaaca acggtgtccg ctcattagat aatgttaccg tatcaaccat
      601 cacttcccac aaggccggat atatcgacaa ggaaaacgaa ctgatcgtcg gcctgcagac
      661 agacgaactt ctgaaacgtg ccatcaagcc tttcggaggt atcaacgtag tcagcaaagc
      721 ctgtcacgaa aacggcgtgg aagtggacga ccgcgtaaaa gatattttca ctcactaccg
      781 caagacgcac aacgacggag tattcgacgt atatacggaa gaaatccgtt ccttccgttc
      841 gctgggattc cttaccggac ttcccgacaa ctatgcacgc ggacgcatca tcggtgacta
      901 ccgccgtatg gctctttacg gcatcgaccg tctgatcgaa gcaaagaagg aagatttgca
      961 caacctcacc ggtccgatga cagatgcccg tatccgtctg cgcgaagaag tggcagaaca
     1021 gatcaaggca ctgaaagaca tgaaagtgat gggcgaatac tacggtctcg acctgagccg
     1081 tcccgcttac acggcacaag aagccgtaca gtgggtatat atggcttacc ttgccgccgt
     1141 caaagaacaa gacggtgccg ccatgtcact gggtaacgtt tcttctttcc tcgatatcta
     1201 tctggaatat gaactgagca aaggaaccat caccgaatcg ttcgcgcaag agctgatcga
     1261 ccagtttgtc atcaaactgc gtatggtccg ccacctgcgt atgcaatcat acaacgacat
     1321 ctttgcaggc gacccgacct gggtaaccga atctctgggc ggacgtctca acgacggacg
     1381 taccaaagtg acaaagacct ccttccgttt cctgcagaca ttgtacaacc tcggcccttc
     1441 accggaaccg aacctgaccg tactctggag cccggaactt cccgaaggat tcaaagaatt
     1501 ctgtgcaaag gtttctatcg acacttcttc tatccagtac gaaaacgacg acctgatgcg
     1561 tgaagtacgt cagtccgacg actacggaat cgcctgctgc gtatcatacc aggaaatagg
     1621 caagcagatt cagttcttcg gtgcccgttg caacctggcc aaagccctgt tgcttgccat
     1681 caacggcgga cgttgcgaga ataccggtac agtcatggtg aagaacatcc ccgtactgac
     1741 cagtgacacg ctgaagttcg aagaagtaat ggacaactac aagaaagtac tgatcgaaat
     1801 cgcccgcgta tacaacgaag cgatgaacat catccactat atgcacgaca agtattatta
     1861 cgagaaagcc cagatggctc ttgtagatac gaacccgcgc ataaaccttg cttacggtgt
     1921 agccggactt tccatcgcac tcgattcact gtctgccatc aaatatgcga aagtaactgc
     1981 ccgccgcaac gatatcggtc tgacagaagg cttcgacatc gaaggagaat tcccttgctt
     2041 cggtaacgac aacgacaagg tagaccacct tggcgtagac ctggtatact tcttcagcga
     2101 agaattgaag aaattgcctg tttacaagaa tgcccgtcct accctctcct tgctgactat
     2161 cacttccaat gtgatgtacg gcaaaaagac cggtgctact cccgacggac gtgccaaagg
     2221 tgttgccttc gctccgggtg ccaacccgat gcacggacgc gacaagaatg gtgcgatcgc
     2281 ttctctgagc tctgtagcga aacttcgtta ccgtgactca caggacggta tcagcaacac
     2341 cttctctatc gttccgaaat cactgggtgc tacggacgaa gaccgtatcg agaacctcgt
     2401 aacgatgatg gacggttact tcaccaaagg tgcccaccac ctgaacgtaa acgtactgaa
     2461 ccgtgatatg ctatacgatg ccatggagca tccggagaac tatccgcagc tgaccatccg
     2521 cgtttccggt tatgccgtaa acttcgtgaa gttgagccgc gaacaccaac tggaagttat
     2581 cagccgtagc ttccacgaac gtatgtaatc tattactcta tatgatgata aacgtacatt
     2641 catacgaaag tatgggaaca ttcgacgggc cgggcttacg gctcgtcgtt tttcttcaag
     2701 gatgcaactt ccgctgcctc tattgcgcca atccggatac gatagcagga aagggaggca
     2761 cgcctacccc accggaagaa atcgtccgca tggcaatgag ccagcgcccc ttcttcggga
     2821 aacgaggagg aatcactttc tctggaggcg aaccgacgtt tcaggcgaaa gcactcgtcc
     2881 cgctggtccg cgaactgaaa gaaaaaggca tccatgtctg tctggacagc aacggaggac
     2941 tttggaacga agacgtagaa gaattattca agctgaccga tcttgtgtta ttggatataa
     3001 aggaattcaa tcccaaccgc catcagacgc tcaccggaag aagcaacgag caaaccatcc
     3061 gcacggccgc ctggctggaa gaacagggaa aacctttctg gctgcgttat gtgctagtgc
     3121 ccggatacag cgattttgaa gaagacatac gcgcactggg agaagcactc ggaaaataca
     3181 agatgatcca acgggtggaa atactgcctt accatacact gggcgtacat aagtacgaag
     3241 cgatgggaca ggaatacaag atgaaagggg taaaagaaaa tacccccgaa cagcttgaaa
     3301 aagctgcgga ggtattcaaa gagtatttca ctacggtagt agttaattaa tggagaattc
     3361 tccaatgacc tcaaagcaag ggcattcttt aatccgttcc
//
