LOCUS       c00022_gnlPro..         5697 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JAJNCFGE_22.
ACCESSION   c00022_gnlPro..
VERSION     c00022_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:31:36
            Original ID  :: gnl|Prokka|JAJNCFGE_22
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5697
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1266
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg22_1"
                     /sec_met_domain="Gly_radical (E-value: 4.8e-42, bitscore:
                     131.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.3e-128, bitscore:
                     416.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TLEVGGIGMDGRSMVTKNCYRFLHTLENMGPAPEPNLTVLYSSAL
                     PEAFKKYAAKVSVNTSSVQYENDDVMKPVWGDDYSICCCVSATQTGKEMQFFGARANLA
                     KCLLYAINGGVDEKSHEQCGPNYAPITSEYLTYDEVLPKYVQMLDWLAGLYVNVLNLIQ
                     YMHDKYYYEEAEMALIDTDVRRTFATGIAGFSHVIDSLSAIKYAKVKVVRDENGLATGF
                     ETEGDFPKYGNDDDRADEIGVWLLKTFLEMIKKRHTYRNSEATTSILTITSNVVYGKYT
                     GALPDGRAAFTPFAPGATPSYGAEQNGLLASLNSVAKLPYHWALDGISNTQTINPEALG
                     HSEGERVENLVQVLDGYFDQGAHHLNVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAV
                     KFIDLTREQQLDVLARTCHGVL"
     protocluster    1..5697
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2005]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2005
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5697
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5697
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1253..2005
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.5;
                     E-value: 1.7e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg22_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.3e-31, bitscore:
                     96.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKTLGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQLDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPNDPAYLAAFDRLMANTNLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWIRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEELLEVSRYPG"
     CDS             2510..3907
                     /locus_tag="ctg22_3"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAVWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLHCGGQDLIFPHHENEIAQSECANGCEFARYWMHNGFINVDNQKMSKSLHNFFTVR
                     DVANLYGYEPIRYFMLTAGYRMPLNYTVDLIESCKNSLERLYTCRENLDFALSKTDFGT
                     DETLKEKAAEARTKFCTAMDDDLNTPDALAAVFDLVKEINTLSAASSKAALETAAAAFD
                     EITGVLGLLYNRKKDEVPAEVTELVEKRAAAKKAKDWATADAIRAQLTELGWAVKDTAQ
                     GPQLSKL"
     CDS             complement(4201..5697)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:Iron-containing alcohol dehydrogenase (Score:
                     334.1; E-value: 2.9e-102)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg22_4"
                     /sec_met_domain="Fe-ADH (E-value: 1.9e-103, bitscore:
                     334.1, seeds: 219, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ERILGKDEVSSSNLDSSSKGKHLEVLGLRGVFLRFFQGRGLVASY
                     CLWKPPKIERLFLPVRQLKLPPNCYIINIIMKYGTALRVPYYFWRFCIMARFTLPRDLY
                     HGKGALEALKSFTGKKAMICVGGGSMKRFGFLDRAVEYLKEAGMEVELFEGIEPDPSVE
                     TVMRGAEAMLKFEPDWIIAMGGGSPIDAAKAMWIKYEYPEITFEEMCKVFGIPPLRRKA
                     HFCAISSTSGTATEVTAFSIITDYQKGIKYPIADFEITPDVAIVDPDLAETMPKKLVAH
                     TGMDAMTHAVEAYVSTAHCDYTDPLAIFAIRMIQGDLVDSYNGDMSKRDSMHNAQCLAG
                     MAFSNALLGIVHSMAHKTGAAFADYGAHIIHGAANAMYLPKVIAFNAKDPEAKKRYGVI
                     ADEMKLGGANDDEKVKLLIEYLRGMNDALNIPHCIQHYGPDSYPTEQGFVPENVFLERL
                     PEIAKNAIADACTGSNPRQPSQEEMEKLLKCCYYDTEVDF"
ORIGIN
        1 acgctggaag tgggcggcat cggcatggac ggccgcagca tggtcaccaa gaactgctac
       61 cgcttcctgc acacgctgga aaacatgggc cccgcaccgg agcccaacct gaccgtgctg
      121 tactcctctg ccctgcccga ggcctttaag aagtacgccg ccaaggtttc cgtcaacacc
      181 agcagcgtcc agtacgagaa cgatgacgtg atgaagcccg tctggggcga cgattactcc
      241 atctgctgct gcgtgtctgc cacccagacc ggcaaggaga tgcagttctt cggcgcacgc
      301 gccaaccttg ccaagtgcct gctgtacgcc atcaacggcg gcgtggatga gaagagccac
      361 gagcagtgtg gccccaacta cgctcccatc accagcgagt acctgaccta tgacgaggtg
      421 ctgcccaagt atgtgcagat gctggactgg ctggccgggc tgtacgtcaa cgtgctgaac
      481 ctcatccagt atatgcacga caagtactac tacgaggaag ccgagatggc actcatcgac
      541 accgatgtgc gccgcacctt tgccaccggc attgcaggct tctcccatgt catcgactct
      601 ctgagcgcca tcaagtacgc caaggtgaag gtggtgcgcg atgaaaacgg ccttgccacc
      661 ggctttgaga ccgagggcga cttccccaag tacggcaacg atgacgaccg cgccgacgag
      721 atcggcgtgt ggctgctcaa gaccttcctt gagatgatca agaagcgcca cacctaccgc
      781 aactccgagg ctaccacctc catcctgacc atcacctcca acgtggtgta cggcaagtac
      841 accggcgctc tgccggatgg ccgcgccgcc ttcaccccgt ttgcgcccgg tgctaccccc
      901 agctacggtg ccgagcagaa cggcctgctg gcctcgctga actccgtggc aaagctgccc
      961 taccactggg cactggacgg catctccaac acccagacca tcaaccccga ggcactgggt
     1021 cacagcgagg gcgagcgcgt ggagaatctg gtgcaggtac tggacggcta cttcgatcag
     1081 ggcgcacacc acctgaacgt gaacgtcttt ggcaaggaga agctgctgga cgccatggag
     1141 caccccgaga aggaagagta cgccaacttc accatccgcg tttccggcta cgccgtcaag
     1201 ttcatcgacc tgacccgcga gcagcagctg gacgtgctgg cccgtacctg ccatggcgtc
     1261 ctgtaagacc cttgggtacg tccactcgct ggaatccttc ggctcggtgg acggccccgg
     1321 cgtgcgcttt gtggtgtttt tgcaggggtg tgccctgcgg tgcaagtact gccacaaccc
     1381 cgaaacatgg gcagagggcg gcgaggcgtg gacggcagaa gcgctgttcc agcgggtata
     1441 ccgctaccgc aactactggg gcaaaaaggg cggcatcacg gtcagcggcg gcgagccgct
     1501 gcggcagctg gactttctga ccgagttctt cacgctggcg cgtgccaagg gcgtgcacac
     1561 agcgctggac accgccgggc agcccttccg cccgaatgac ccggcctatc tggccgcatt
     1621 tgaccgcctg atggctaaca ccaatctggt gatcttagac ctcaaggaga tcgacccgga
     1681 gcgccaccgg cagctcaccg gcaaggacaa tgccaacatc cttgccatgg cgcggcacat
     1741 ctccgacctc ggcatcccgc tgtggatccg gcacgtcctt gtgcccggtc tgaccgatga
     1801 cgaggaaggg ctgcgcaaaa cagcagactt tatccgcagc ctgaaaacgg tgcagcgggt
     1861 ggaggtgctg ccctaccaca ccttggggct gttcaagtgg cagaagctgg gcattcccta
     1921 tcccctgccg gacgcagtac cgcccacggc agagcaggta aagcgggcag aagagctgct
     1981 ggaagtaagc cggtatcccg ggtaaagcac caccgttttg attgcgctcc gcttcgctct
     2041 gcacccttcc cgtgaaaatg ggatatcgga acgcccgcag gcgttccgat atcccgaaat
     2101 tcagaataat tattccgctg aggatgccca aagcaacgcg gagggcattc tgaatcgttc
     2161 tttgttcctc cttgacttcc ctgcacagca ccgctataat aaaggggtaa aaggataaat
     2221 gcgtttatcc gggcagagta gcgccgcaca ccgtctgcgc agagagaaca ggccactggc
     2281 tggaagcctg tttgcagacc gccgcgcaaa gtgcgcccgg acagcacgcg gcgggaaccc
     2341 cgtggggaag tatcgccgtg cggtgcgccg ccgttaccgg ctttgagcga caaacagaag
     2401 tggaaccgcg attttgattt taaaaccgcc ttcggagctg tgccttgtgc accgctccgg
     2461 gggcgttttg ttttgcggct tctgtttgga acaaaaagga gaattgacca tgcagatctt
     2521 caacaccctg acccgacaga aggaagaatt tgtgccgcag gtgcccggcg agtaccgcat
     2581 ctacgtctgc ggccccaccg tgtacaacta tatccacatt ggcaacgcac gcccgctgat
     2641 cgtgttcgac accctgcgcc gctatctgga ataccggggc aacaaggtga tctacgtttc
     2701 caacatcacc gatatcgacg ataagctcat caagaagggg caggaagagg gcacctccat
     2761 gaaggaggtg gcgcagcgct ttgaggccga gtacctgaag gatgccgccg gtctgaactg
     2821 tgaaaagccc accgtgcagc cccgcgctac cgagcatatc ccccagattc tcgatatcgt
     2881 caaagatctg atcgactccg gccacgccta cgttgccaag aacggcgatg tgtacttccg
     2941 ggtgaagagc gaccccggct acggcaagct gagccacctg aatctggacg atctggaaag
     3001 cggcaaccgc gagctgcgca gccgcatgga ggacgatctg aaggaggatc ccgccgactt
     3061 tgccgtctgg aaggccgcaa agcccggtga acccgcatgg gaaagcccct acggcatggg
     3121 ccgcccgggc tggcacattg agtgcagcgc catgagccgc acccatctgg gcaagaccat
     3181 cgacctgcac tgcggcggtc aggacctgat cttcccccac cacgaaaacg agatcgccca
     3241 gagcgagtgc gccaacggct gtgagtttgc ccgctactgg atgcacaacg gtttcatcaa
     3301 cgtggacaat cagaagatgt ccaagagcct gcacaacttc tttaccgtgc gggatgtagc
     3361 aaacctctac ggctacgagc ccatccgcta cttcatgctg accgccggct accggatgcc
     3421 cctgaactac accgtagacc tcatcgagag ctgcaagaac agtctggagc gcctgtacac
     3481 ctgccgcgag aatctggact ttgccctgag caagaccgac tttggcaccg atgagacttt
     3541 gaaggaaaag gccgccgagg cacgcaccaa gttctgcacc gccatggacg acgacctgaa
     3601 caccccggat gcgctggctg ccgtgtttga tctggtgaag gagatcaaca ccctgtctgc
     3661 tgcttccagc aaggctgcgc tggagactgc tgccgccgcc tttgacgaga tcaccggtgt
     3721 gctgggtctg ctgtacaacc gcaagaagga tgaggtgccc gccgaggtga ctgagctggt
     3781 ggagaaacgt gctgctgcca agaaggccaa ggactgggcc accgccgatg ccatccgcgc
     3841 acagctgacc gaactgggct gggctgtcaa ggacaccgca cagggtcccc agctgagcaa
     3901 gctgtaagct ttgctcagct ggcgggagga ccattttaaa tggcctccgc gctgctctgg
     3961 ccatagttca aggaattata atttagaatt gggggttcag aaacgcctgc gggcgtttct
     4021 gaaccccttt ttcacagctg gggtctcaat cccgtgaaaa tgcaatgccg gagcgtccgc
     4081 agacgctccg gcattgctct attaaaaata atttttccgc aacggcaaaa gccccgcagg
     4141 gcggtatgct ctgcggggct tttgtccata aggaaggagg tgtaataaag aaaggacgaa
     4201 tcagaaatcc acctcagtat catagtagca gcacttgagc agcttctcca tctcctcctg
     4261 actgggctgg cgggggttgg agccggtgca ggcgtcggcg atggcgttct tggcgatctc
     4321 gggcagacgc tcgaggaaca cattctccgg cacaaagccc tgctcagtag ggtaggagtc
     4381 cgggccgtag tgctggatgc agtgggggat gttcagcgca tcgttcatgc cgcgcaggta
     4441 ctcgatgagc agtttgacct tttcgtcatc gtttgcgccg cccagcttca tttcgtccgc
     4501 gatgacgccg tagcgcttct tagcctcggg gtctttggcg ttgaaggcga tgaccttggg
     4561 caggtacatg gcgttagcag caccgtggat gatgtgtgcg ccgtagtctg caaaggcagc
     4621 gccggtcttg tgtgccatgg agtgcacgat gcccagcagt gcgttggaga aggccatacc
     4681 ggcaaggcac tgtgcgttgt gcatggagtc gcgcttggac atatcaccgt tgtagctgtc
     4741 caccagatcg ccctggatca tgcggatggc gaagatggcc agaggatcgg tgtagtcgca
     4801 gtgtgcggtg gaaacataag cctccacggc atgggtcatg gcgtccatac cagtgtgggc
     4861 aaccagcttt ttgggcatgg tctcagccag atcggggtcc acgatggcca cgtccggggt
     4921 gatctcaaag tctgcgatgg ggtacttgat gcccttctgg tagtcggtga taatggagaa
     4981 agcggtcacc tcggtggcag tgccggaggt ggaggaaatg gcgcagaagt gtgccttgcg
     5041 gcgcagcggc gggatgccga acaccttgca catctcctca aaggtgatct cggggtactc
     5101 gtacttgatc cacatggcct tggctgcatc gatgggagag ccgccgccca tggcaatgat
     5161 ccagtcgggc tcaaacttca gcatggcctc ggcaccgcgc atgacggtct ccacggaagg
     5221 atcgggttcg atgccctcga acagctccac ttccatgccg gcttccttca ggtactccac
     5281 agcacggtcc aagaagccga aacgcttcat ggagccgccg ccgacgcaga tcattgcctt
     5341 tttgccggtg aaggacttca gcgcttccag agcgcccttg ccatggtaca gatcacgggg
     5401 cagagtaaaa cgagccataa tacaaaacct ccaaaaatag tacggcacac gcagcgctgt
     5461 gccatatttc attattatgt taattatata acaatttgga ggcagcttca attgacgtac
     5521 cgggagaaaa agtcgttcta ttttgggcgg tttccacaag cagtaggatg caacaagccc
     5581 cctgccctga aaaaagcgca aaaaaacacc ccgaagtcct aagacttcga ggtgcttgcc
     5641 tttggagctg ctatccagat tcgaactgga gacctcatcc ttaccaagga tgcgctc
//
