LOCUS       c00064_gnlPro..         3467 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KODPHNPI_64.
ACCESSION   c00064_gnlPro..
VERSION     c00064_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:13:45
            Original ID  :: gnl|Prokka|KODPHNPI_64
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3467
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1614
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg64_1"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-42, bitscore:
                     132.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 6.9e-188, bitscore:
                     613.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LREEIARQISALKGMKKMAEAYGYDISQPAKDAKEACQWLYFGYL
                     AAIKTQNGAAMSVGRISTFLDIYIQRDLDRGILTESQAQELIDHMVMKFRMVKFARIPS
                     YNQLFSGDPVWATLEVGGIGMDGRSMVTKNCYRFLHTLENMGPAPEPNLTVLYSSALPE
                     AFKKYAAKVSVNTSSVQYENDDVMKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKC
                     LLYAINGGVDEKSHEQCGPNYAPITSEYLTYDEVLPKYVQMLDWLAGLYVNVLNLIQYM
                     HDKYYYEEAEMALIDTDVRRTFATGIAGFSHVIDSLSAIKYAKVKVVRDENGLATGFET
                     EGDFPKYGNDDDRADEIGVWLLKTFLEMIKKRHTYRNSEATTSILTITSNVVYGKYTGA
                     LPDGRAAFTPFAPGATPSYGAEQNGLLASLNSVAKLPYHWALDGISNTQTINPEALGHS
                     EDERVENLVQVLDGYFDQGAHHLNVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAVKF
                     IDLTREQQLDVLARTCHGVL"
     protocluster    1..3467
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2353]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2353
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3467
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3467
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             1601..2353
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.3;
                     E-value: 1.9e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg64_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.6e-30, bitscore:
                     94.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKALGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQLDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPDDPAYLAAFDRLMANTSLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWVRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEELLEVSRYPG"
     CDS             2773..3465
                     /locus_tag="ctg64_3"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAVWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLHCGGQ"
ORIGIN
        1 ctgcgcgagg agatcgcccg ccagatcagc gccctgaagg gcatgaagaa gatggccgag
       61 gcttacggct acgacatctc ccagcccgcc aaggacgcca aggaggcctg ccagtggctg
      121 tacttcggct atctggccgc catcaagacc cagaacggcg ccgccatgag cgtgggccgc
      181 atctccacct tccttgacat ttatatccag cgcgatctgg acaggggcat cctgaccgaa
      241 agtcaggcac aggagctcat cgaccacatg gtcatgaagt tccgcatggt caagtttgcc
      301 cgcatcccca gctacaacca gctgttctcc ggcgaccccg tgtgggcgac cctggaggtg
      361 ggcggcatcg gcatggacgg ccgcagcatg gtcaccaaga actgctaccg cttcctgcac
      421 acgctggaaa acatgggccc cgcaccggag cccaacctga ccgtgctgta ctcctctgcc
      481 ctgcccgagg cctttaagaa gtacgccgcc aaggtgtccg tcaacaccag cagcgtccag
      541 tacgagaacg acgatgtgat gaagcccgtc tggggcgacg attactccat ctgctgctgc
      601 gtgtctgcca cccagaccgg caaggagatg cagttcttcg gcgcacgcgc caaccttgcc
      661 aagtgcctgc tgtacgccat caacggcggc gtggatgaga agagccacga gcagtgcggc
      721 cccaactacg cccccatcac cagcgagtac ctgacctatg acgaggtgct gcccaagtat
      781 gtgcagatgc tggactggct ggccgggctg tacgtcaacg tgctgaacct catccagtat
      841 atgcacgaca agtactacta cgaggaagcc gagatggcac tcatcgacac cgatgtgcgc
      901 cgcacctttg ccaccggcat tgcaggcttc tcccatgtca tcgactccct gagcgccatc
      961 aagtacgcca aggtgaaggt ggtgcgcgac gagaacggcc ttgccaccgg ctttgagacc
     1021 gagggcgact tccccaagta cggcaacgat gacgaccgcg ccgacgagat cggcgtgtgg
     1081 ctgctcaaga ccttccttga gatgatcaag aagcgccaca cctaccgcaa ctccgaggct
     1141 accacctcca tcctgaccat cacctccaac gtggtgtacg gcaagtacac cggcgctctg
     1201 ccggatggcc gcgccgcctt caccccgttt gcgcccggtg ctacccccag ctacggcgca
     1261 gagcagaacg gtctgctggc ctcgctgaac tccgtggcaa agctgcccta ccactgggcg
     1321 ctggacggca tctccaacac ccagaccatc aaccccgagg cactgggtca cagcgaggac
     1381 gagcgcgtgg agaatctggt gcaggtactg gatggctact tcgatcaggg cgcacaccac
     1441 ctgaacgtga atgtctttgg caaggagaag ctgctggacg ccatggagca ccccgagaag
     1501 gaagagtacg ccaacttcac catccgcgtt tccggctacg ctgtcaagtt tatcgacctg
     1561 acccgcgagc agcagctgga cgtgctggca cgcacctgcc atggcgtcct gtaaggccct
     1621 tgggtacgtc cactcgctgg aatccttcgg ctcggtggac ggccccggcg tgcgctttgt
     1681 ggtgtttttg caggggtgtg ccctgcggtg caagtactgc cacaaccccg aaacatgggc
     1741 agagggcggc gaggcgtgga cggcagaagc gctgttccag cgggtatacc gctaccgcaa
     1801 ctactggggc aaaaagggcg gcatcacggt cagcggcggc gagccgctgc ggcagctgga
     1861 ctttttgact gagttcttca cgctggcgcg tgccaagggc gtgcacacgg cgctggacac
     1921 cgccgggcag cctttccgcc cggacgaccc ggcgtatctg gcggcgttcg accgtctgat
     1981 ggctaacacc agtctggtga tcttagacct caaggagatc gacccggagc gccaccggca
     2041 gctcaccggc aaggacaacg ccaacatcct tgccatggcg cggcacatct ccgacctcgg
     2101 catcccgctg tgggtgcggc acgtccttgt gcccgggctg accgatgacg aggaggggct
     2161 gcgcaaaacg gctgacttta tccgctcgct caaaacggtg cagcgggtgg aggtgctgcc
     2221 ctaccacacc ttggggctgt tcaagtggca aaagctgggc atcccctatc ccctgccgga
     2281 cgctgtaccg cccacggcag agcaggtaaa gcgggcagaa gaactgctgg aagtgagccg
     2341 gtatcccggg taaagaggaa agcatctttt tgatttgaaa ttcagccaag ccggtaggct
     2401 aggctgaatt tcattttcac tggttcgttc tgccttgact tccctgcacc gcaccgctat
     2461 aataaagggg taaaaggata aatgcgttta tccgggcaga gtagcgccgc acaccgtctg
     2521 cgcagagaga acaggccact ggctggaagc ctgtttgcag accgccgcgc aaagtgcgcc
     2581 cggacagcac gcggcgggaa ccccgtgggg aagtatcgcc gtgcggtgcg ccgccgttac
     2641 cggctttgag cgacaaacag aagtggaacc gcgattttga ttttaaaacc gccttcggag
     2701 ctgtgccttg tgcaccgctc cgggggcgtt ttgttttgcg gcttctgttt ggaacaaaaa
     2761 ggagaattga ccatgcagat cttcaacacc ctgacccgac agaaggaaga atttgtgccg
     2821 caggtgcccg gcgagtaccg catctacgtc tgcggcccta ccgtgtacaa ctatatccac
     2881 atcggcaacg cacgcccgct gatcgtgttc gacaccctgc gccgctatct ggaataccgg
     2941 ggcaacaagg tgatctacgt ttccaacatc accgatatcg acgataagct catcaaaaag
     3001 ggacaggaag agggcacctc catgaaggag gtggcacagc gctttgaggc cgagtacctg
     3061 aaggatgccg ccgggctgaa ctgcgaaaag cccactgtgc agccccgcgc taccgagcat
     3121 atcccccaga ttttggatat cgtcaaggat ctgatcgact ccggccacgc ctacgttgcc
     3181 aaaaacggcg atgtgtactt ccgggtgaag agcgaccccg gctacggcaa gctgagccac
     3241 ctgaatctgg acgatctgga aagcggcaac cgcgagctgc gcagccgcat ggaggacgat
     3301 ctgaaggagg atcccgccga ctttgccgtc tggaaggccg caaagcccgg tgaacccgca
     3361 tgggaaagcc cctacggcat gggccgcccg ggctggcaca ttgagtgcag cgccatgagc
     3421 cgcacccatc tgggcaagac catcgacctg cactgcggcg gtcagga
//
