LOCUS       c00566_gnlPro..         7165 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AOHANEMP_566.
ACCESSION   c00566_gnlPro..
VERSION     c00566_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 10:06:49
            Original ID  :: gnl|Prokka|AOHANEMP_566
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7165
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7165
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3989:6981]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3990..6981
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7165
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7165
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             612..1829
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:Iron-containing alcohol dehydrogenase (Score:
                     335.6; E-value: 1e-102)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg566_1"
                     /sec_met_domain="Fe-ADH (E-value: 6.5e-104, bitscore:
                     335.6, seeds: 219, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARFTLPRDLYHGKGALEALKSFTGKKAMICVGGGSMKRFGFLDR
                     AVEYLKEAGMEVELFEGIEPDPSVETVMRGAEAMLKFEPDWIIAMGGGSPIDAAKAMWI
                     KYEYPEITFEEMCKVFGIPPLRRKAHFCAISSTSGTATEVTAFSIITDYQKGIKYPIAD
                     FEITPDVAIVDPDLAETMPKKLVAHTGMDAMTHAVEAYVSTAHCDYTDPLAIFAIRMIQ
                     GDLVDSYNGDMSKRDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHIIHGAA
                     NAMYLPKVIAFNAKDPEAKKRYGVIADEMKLGGANDDEKVKLLIEYLRGMNDALNIPHC
                     IKHYGPDSYPTEQGFVPENVFLERLPEIAKNAIADACTGSNPRQPSQEEMEKLLKCCYY
                     DTEVDF"
     CDS             complement(2172..3569)
                     /locus_tag="ctg566_2"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAVWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLHCGGQDLIFPHHENEIAQSECANGCEFARYWMHNGFINVDNQKMSKSLHNFFTVR
                     DVANLYGYEPIRYFMLTAGYRMPLNYTVDLIESCKNSLERLYTCRENLDFALSRTDFGT
                     DETLKEKAAEARTKFCTAMDDDLNTPDALAAVFDLVKEINTLSAASSKAALETAAAAFD
                     EITGVLGLLYNRKKDEVPAEVTALVEKRAAAKKAKDWATADAIRAQLTELGWAVKDTAQ
                     GPQLSKL"
     CDS             complement(3990..4742)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.7;
                     E-value: 1.4e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg566_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-30, bitscore:
                     94.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKTLGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQMDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPDDPAYLAAFDRLMANTSLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWIRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEDLLEVSRYPG"
     CDS             complement(4729..6981)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg566_4"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-41, bitscore:
                     129.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-267, bitscore:
                     876.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIQFEQWEGFEGRIWKEEVNVRDFIQKNYTPYDGDESFLAGPTEA
                     TDKLWGALQQLQKAERAKGGVLDMETEVVSSLTSYGPGYIDESLKDLEQIVGLQTDKPL
                     KRAFMPYGGIKMAEQACTTYGYQPSAELHKIFTDYTRTHNQAVFDAYTPEMKKARHTHI
                     ITGLPDTYGRGRIVGDYRRVALYGIDTLIQFKQEDLANCGDGTMTDDVIRLREEIARQI
                     SALKGMKKMAEAYGYDISQPAKDAKEACQWLYFGYLAAIKTQNGAAMSVGRISTFLDIY
                     IQRDLDRGILTESQAQELIDHMVMKFRMVKFARIPSYNQLFSGDPVWATLEVGGIGMDG
                     RSMVTKNCYRFLHTLENMGPAPEPNLTVLYSSALPEAFKKYAAKVSVNTSSVQYENDDV
                     MKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEKSHEQCGPNYAP
                     ITSEYLTYDEVLPKYVQMLDWLAGLYVNVLNLIQYMHDKYYYEEAEMALIDTDVRRTFA
                     TGIAGFSHVIDSLSAIKYAKVKVVRDESGLATGFETEGDFPKYGNDDDRADEIGVWLLK
                     TFLEMIKKRHTYRNSEATTSILTITSNVVYGKYTGALPDGRAAFTPFAPGATPSYGAEQ
                     NGLLASLNSVAKLPYHWALDGISNTQTINPEALGHSEGERVENLVQVLDGYFDQGAHHL
                     NVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVLARTCHGVL"
ORIGIN
        1 ggggtatagc tcagctggta gagcagcggt ctccaaaacc gcgtgccgtg agttcgaaac
       61 ttactacccc tgccatggcg cacaggttct tcggaacttg tgcgttttgt tttttggccc
      121 gcttttgcaa aaaattgcat tttccgcaaa tttgtgcttg acaacagacc gactttccga
      181 tataatagag cacgttcccg cggtaagatg ccgcacgaac ttgagcgatc atggcccggt
      241 agctcagttg gttagagcac cagcctgtca cgctgggggt cgtcggttcg agcccgatcc
      301 gggtcgccat ttgctgctat agctcagttg gtagagcgca tccttggtaa ggatgaggtc
      361 tccagttcga atctggatag cagctccaaa ggcaagcacc tcgaagtctt aggacttcgg
      421 ggtgtttttt tgcgcttttt cagggcaggg ggcttgttgc atcctactgc ttgtggaaac
      481 cgcccaaaat agaacgactt tttctcccgg tacgtcaatt gaagccatct ccaaattgtt
      541 atataattaa cataatgatg aactgcggta cagcgctgcg tgtgccgtac tatttttgga
      601 ggttttgtat tatggctcgt tttactctgc cccgtgatct gtaccatggc aagggcgctc
      661 tggaagcgct gaagtccttt accggcaaaa aggcaatgat ctgcgtcggc ggcggctcca
      721 tgaagcgctt cggcttcctg gaccgtgccg tggagtacct gaaggaagcc ggcatggaag
      781 tggagctgtt cgagggcatc gaacccgatc cttccgtgga gaccgtcatg cgcggtgccg
      841 aggcgatgct gaagtttgag cccgactgga tcatcgccat gggcggcggc tctcccatcg
      901 atgcagccaa ggccatgtgg atcaagtacg agtaccccga gatcaccttt gaggagatgt
      961 gcaaggtgtt cggcatcccg ccgctgcgcc gcaaggcaca cttctgcgcc atttcctcca
     1021 cctccggcac tgctaccgag gtgaccgctt tctccatcat caccgactac cagaagggca
     1081 tcaagtaccc catcgcggac ttcgagatca ccccggacgt ggccatcgtg gacccggatc
     1141 tggctgagac catgcccaag aagctggttg cccacaccgg tatggacgcc atgactcacg
     1201 ctgtggaggc ttatgtttcc accgcacact gcgactacac cgacccgctg gccatcttcg
     1261 ccatccgcat gatccagggc gatctggtgg acagctacaa tggcgatatg tccaagcgcg
     1321 actccatgca taacgcccag tgccttgccg gtatggcttt ctccaacgca ctgctgggca
     1381 tcgtgcactc catggcacac aagaccggcg ctgcctttgc agactacggc gcacacatca
     1441 tccacggtgc ggccaacgcc atgtacctgc ccaaggtcat cgccttcaac gccaaggacc
     1501 cggaggccaa gaagcgctac ggcgtcatcg cagacgagat gaagctgggc ggcgcaaacg
     1561 atgacgagaa ggtcaagctg cttatcgagt acctgcgcgg catgaacgat gcgctgaaca
     1621 tcccccactg catcaagcac tacggcccgg actcctaccc cactgagcag ggctttgtgc
     1681 cggagaatgt gttcctggag cgtctgcccg agatcgccaa gaacgccatc gccgacgcct
     1741 gcaccggctc caacccccgc cagcccagcc aggaggagat ggagaagctg ctcaagtgct
     1801 gctactacga tactgaggtg gatttctgat ccgtcctttc tttagtacgc ctccttccct
     1861 atggacaaaa gccccgcaga gcataccgcc ctgcggggct tttgccgttt atgccaaacg
     1921 atttaaaatg cccgccgcgt gcgctttggg catattcagc ggaaaaatta tttttaatag
     1981 agcaatgccg gagcgtctgc ggacgctccg gcattgcatt ttcacgggat tgagacccca
     2041 gctgtgaaaa aggggttcag aaacgcccgc aggcgtttct gaacccccaa ttctaaatta
     2101 taattccttg aactatggcc agagcagcgc ggaggccatt caaaatggtc cccccgccag
     2161 ctgagcaaag cttacagctt gctcagctgg ggaccctgtg cggtgtcctt aacagcccag
     2221 cccagttcgg tcagctgtgc gcggatggca tcagcggtgg cccagtcctt ggcctttttg
     2281 gcagcagcgc gcttttccac cagcgcggtc acctcggcgg gcacctcgtc cttcttgcgg
     2341 ttgtacagca gacccagcac accggtgatc tcgtcaaagg cggcggcagc ggtctccagt
     2401 gcggccttgc tggaagcagc ggacagggtg ttgatctcct tgaccagatc gaacacggca
     2461 gccagcgcat ccggggtgtt caggtcgtcg tccatggcgg tgcagaactt ggtgcgtgcc
     2521 tctgcggcct tttccttcag ggtctcgtcg gtgccaaagt cggtcctgct cagggcaaag
     2581 tccagattct cgcggcaggt gtacaggcgc tccaggctgt tcttgcagct ctcgatgagg
     2641 tctacggtgt agttcagggg catgcggtag ccggcggtca gcatgaagta gcggatgggc
     2701 tcgtagccgt agaggtttgc cacatcccgc acggtaaaga agttgtgcag gctcttggac
     2761 attttctggt tgtccacgtt gatgaaaccg ttgtgcatcc agtagcgggc gaactcgcag
     2821 ccgttggcgc actcgctctg ggcgatctcg ttttcgtggt gggggaagat cagatcctga
     2881 ccgccgcagt gcaggtcgat ggtcttgccc agatgggtgc ggctcatggc gctgcactca
     2941 atgtgccagc ccgggcggcc catgccgtag gggctttccc atgcgggctc accgggcttt
     3001 gcagccttcc agacggcaaa gtcggcggga tcttccttca gatcgtcctc catgcggctg
     3061 cgcagctcgc ggttgccgct ctccagatcg tccagattca ggtggctcag cttgccgtag
     3121 ccggggtcgc ttttcacccg gaagtacaca tcgccgtttt tggcaacgta ggcgtggccg
     3181 gagtcgatca aatccttgac gatatccaaa atctggggga tatgctcggt agcgcggggc
     3241 tgcacggtgg gcttttcgca gttcagaccg gcggcatcct tcaggtactc ggcctcaaag
     3301 cgctgcgcca cctccttcat ggaggtgccc tcttcctgtc ccttcttgat gagcttatcg
     3361 tcgatatcgg tgatgttgga aacgtagatc accttgttgc cccggtattc cagatagcgg
     3421 cgcagggtgt cgaacacgat cagcgggcgt gcgttgccga tgtggatata gttgtacacg
     3481 gtagggccgc agacgtagat gcggtactcg ccgggcacct gcggcacaaa ttcttccttc
     3541 tgtcgggtca gggtgttgaa gatctgcatg gtcaattctc ctttttgttc caaacagaag
     3601 ccgcaaaaca aaacgccccc ggagcggtgc gcaaggcaca gctccgaagg cggttttaaa
     3661 atcaaaatcg cggttccact tctgtttgtc gctcaaagcc ggtaacggcg gcgcaccgca
     3721 cggcgatact tccccgcagg ggttcccgcc gcgtgctgtc cgggcgcact ttgcgcagcg
     3781 gtctgcaaac aggcttccag ccagtggcct gttctctctg cgcagacggt gtgcggcgct
     3841 actctgcccg gataaacgca tttatccttt taccccttta ttatagcggt gcggtgcagg
     3901 gaagtcaagg cagaacgaac cagtgaaaat gaaattcagc caagcctacc ggcttggctg
     3961 aatttcaaat caaaaagatg ctttcctctt tacccgggat accggctcac ttccagcaga
     4021 tcctctgcgc gctttacctg ctctgccgtg ggcggtacag cgtccggcag gggatagggg
     4081 atgcccagct tttgccactt gaacagcccc aaggtgtggt agggcagcac ctccacccgc
     4141 tgcaccgttt tgagcgagcg gataaagtct gctgttttgc gcagcccttc ctcgtcatcg
     4201 gtcagaccgg gcacgaggac gtgccggatc cacagcggga tgccgaggtc ggagatgtgc
     4261 cgcgccatgg caaggatgtt ggcattgtct ttgccggtga gctgccggtg gcgctccggg
     4321 tcgatctctt tgaggtctaa gatcaccaga ctggtgttag ccatcaggcg gtcgaacgcg
     4381 gccagatagg ccgggtcatc cgggcggaag ggctgcccgg cggtgtccag cgccgtgtgc
     4441 acgcccttgg cgcgtgccag cgtgaagaac tcagtcagaa aatccatttg ccgcagcggc
     4501 tcgccgccgc tgaccgtgat gccgcccttt ttgccccagt agttgcggta gcggtatacc
     4561 cgctggaaca gcgcttctgc cgtccacgcc tcgccgccct ctgcccatgt ttcggggttg
     4621 tggcagtatt tgcaccgcag ggcacacccc tgcaaaaaca ccacaaagcg cacgccgggg
     4681 ccgtccaccg agccgaagga ttccagcgag tggacgtacc caagagtctt acaggacgcc
     4741 atggcaggtg cgtgccagca cgtccagctg ctgctcgcgg gtcaggtcga tgaacttgac
     4801 ggcgtagccg gaaacgcgga tggtgaagtt ggcgtactct tccttctcgg ggtgctccat
     4861 ggcatccagc agcttctcct tgccaaagac gttcacgttc aggtggtgtg cgccctgatc
     4921 gaagtagccg tccagcacct gcaccagatt ctccacgcgt tcgccctcac tgtgacccag
     4981 tgcctcgggg ttgatggtct gggtgttgga gatgccgtcc agtgcccagt ggtagggcag
     5041 ctttgccacg gagttcagcg aagccagcag accgttctgc tctgcaccgt agctgggggt
     5101 agcgccgggt gcaaacgggg tgaaggcggc gcggccatcc ggcagggcgc cggtgtactt
     5161 gccgtacacc acgttggagg tgatggtcag gatggaggtg gtagcctcgg agttgcggta
     5221 ggtgtggcgc ttcttgatca tctcaaggaa ggtcttgagc agccacacgc cgatctcgtc
     5281 ggcgcgatcg tcatcgttgc cgtacttggg gaagtcgccc tcggtctcaa agccggtggc
     5341 aaggccgctc tcgtcgcgca ccaccttcac cttggcgtac ttgatggcgc tcagggagtc
     5401 gatgacatgg gagaagcctg caatgccggt ggcaaaggtg cggcgcacat cggtgtcgat
     5461 gagtgccatc tcggcttcct cgtagtagta cttgtcgtgc atatactgga tgaggttcag
     5521 cacgttgacg tacagcccgg ccagccagtc cagcatctgc acatacttgg gcagcacctc
     5581 gtcataggtc aggtactcgc tggtgatggg agcatagttg gggccgcact gctcgtggct
     5641 cttctcatcc acgccgccgt tgatggcgta cagcaggcac ttggcaaggt tggcgcgtgc
     5701 gccgaagaac tgcatctcct tgccggtctg ggtggcagac acgcagcagc agatggagta
     5761 atcgtcgccc cagacgggct tcatcacatc gtcgttctcg tactggacgc tgctggtgtt
     5821 gacggacacc ttggcggcgt acttcttaaa ggcctcgggc agggcagagg agtacagcac
     5881 ggtcaggttg ggctccggtg cggggcccat gttttccagc gtgtgcagga agcggtagca
     5941 gttcttggtg accatgctgc ggccgtccat gccgatgccg cccacctcca gggtcgccca
     6001 cacggggtcg ccggagaaca gctggttgta gctggggatg cgggcaaact tgaccatgcg
     6061 gaacttcatg accatgtggt cgatgagctc ctgtgcctga ctttcggtca ggatgcccct
     6121 gtccagatcg cgctggatat aaatgtcaag gaaggtggag atgcggccca cgctcatggc
     6181 ggcgccgttc tgggtcttga tggcggccag atagccgaag tacagccact ggcaggcctc
     6241 cttggcgtcc ttggcgggct gggagatgtc gtagccgtaa gcctcggcca tcttcttcat
     6301 gcccttcagg gcgctgatct ggcgggcgat ctcctcgcgc aggcggatga cgtcatcggt
     6361 catggtgccg tcgccgcagt tggcaagatc ctcctgcttg aactggatca gggtgtcaat
     6421 gccgtacagg gcaacgcggc ggtagtcgcc cacgatgcgg ccgcgtccat aggtgtctgg
     6481 cagaccggtg atgatgtggg tgtggcgtgc cttcttcatc tccggggtgt aggcatcgaa
     6541 cacggcctga ttgtgggtgc gggtgtagtc ggtaaagatc ttgtgcagct cggcagaggg
     6601 ctggtagccg taggtggtgc aggcctgttc tgccatcttg atgccgccgt agggcataaa
     6661 ggcgcgcttg aggggcttgt cggtctgtaa gccaacgatc tgctccagat ccttcaggct
     6721 ttcgtcaata tagccggggc cgtaggaggt caggctgctg accacctcgg tctccatatc
     6781 cagcacgccg cccttggcgc gctcggcctt ctgcagctgc tgcaaagcac cccaaagctt
     6841 atcggtagcc tcggtggggc ctgccagaaa gctttcgtcg ccgtcatagg gagtgtagtt
     6901 tttctggatg aagtcacgca cgttgacctc ttccttccag atgcggcctt caaagccttc
     6961 ccactgttca aactggatca ttggaacctc cttgctccat tacgcagtta gcgttttcta
     7021 actaatgagc ctttgaaaaa ccgctctgtc tgggcggttt tctgggggcg aaacggtctt
     7081 gcgctgccgt ctgcggcagc ccttactaca aggtgtagac aaaaataaaa acctgcccac
     7141 aaaatcttgc gttactataa tagca
//
