LOCUS       c00604_gnlPro..         5361 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KECICMPC_604.
ACCESSION   c00604_gnlPro..
VERSION     c00604_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 06:11:42
            Original ID  :: gnl|Prokka|KECICMPC_604
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5361
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5361
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3396:5359]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3397..5359
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5361
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5361
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             50..1267
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035:Iron-containing alcohol dehydrogenase (Score:
                     335.1; E-value: 1.4e-102)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg604_1"
                     /sec_met_domain="Fe-ADH (E-value: 9e-104, bitscore: 335.1,
                     seeds: 219, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARFTLPRDLYHGKGALEALKSFTGKKAMICVGGGSMKRFGFLDR
                     AVEYLKEAGMEVELFEGIEPDPSVETVMRGAEAMLKFEPDWIIAMGGGSPIDAAKAMWI
                     KYEYPEITFEEMCKVFGIPPLRRKAHFCAISSTSGTATEVTAFSIITDYQKGIKYPIAD
                     FEITPDVAIVDPDLAETMPKKLVAHTGMDAMTHAVEAYVSTAHCDYTDPLAIFAIRMIQ
                     GDLVDSYNGDMSKRDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHIIHGAA
                     NAMYLPKVIAFNAKDPEAKKRYGVIADEMKLGGANDDEKVKLLIEYLRGMNDALNIPHC
                     IQHYGPDSYPTEQGFVPENVFLERLPEIAKNAIADACTGSNPRQPSQEEMEKLLKCCYY
                     DTEVDF"
     CDS             complement(1427..2824)
                     /locus_tag="ctg604_2"
                     /transl_table=11
                     /translation="MQIFNTLTRQKEEFVPQVPGEYRIYVCGPTVYNYIHIGNARPLIV
                     FDTLRRYLEYRGNKVIYVSNITDIDDKLIKKGQEEGTSMKEVAQRFEAEYLKDAAGLNC
                     EKPTVQPRATEHIPQILDIVKDLIDSGHAYVAKNGDVYFRVKSDPGYGKLSHLNLDDLE
                     SGNRELRSRMEDDLKEDPADFAIWKAAKPGEPAWESPYGMGRPGWHIECSAMSRTHLGK
                     TIDLHCGGQDLIFPHHENEIAQSECANGCEFARYWMHNGFINVDNQKMSKSLHNFFTVR
                     DVANLYGYEPIRYFMLTAGYRMPLNYTVDLIESCKNSLERLYTCRENLDFALSKTDFGT
                     DETLKEKAAEARTKFCTAMDDDLNTPDALAAVFDLVKEINTLSAASSKAALETAAAAFD
                     EITGVLGLLYNRKKDEVPAEVTALVEKRAAAKKAKDWATADAIRAQLTELGWAVKDTAQ
                     GPQLSKL"
     CDS             complement(3397..4149)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 62.6;
                     E-value: 1.5e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg604_3"
                     /sec_met_domain="Radical_SAM (E-value: 4.4e-31, bitscore:
                     96.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASCKTLGYVHSLESFGSVDGPGVRFVVFLQGCALRCKYCHNPET
                     WAEGGEAWTAEALFQRVYRYRNYWGKKGGITVSGGEPLRQLDFLTEFFTLARAKGVHTA
                     LDTAGQPFRPDDPAYLAAFDRLMANTNLVILDLKEIDPERHRQLTGKDNANILAMARHI
                     SDLGIPLWIRHVLVPGLTDDEEGLRKTADFIRSLKTVQRVEVLPYHTLGLFKWQKLGIP
                     YPLPDAVPPTAEQVKRAEELLEVSRYPG"
     CDS             complement(4136..5359)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg604_4"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-42, bitscore:
                     132.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5e-122, bitscore:
                     396.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VTKNCYRFLHTLENMGPAPEPNLTVLYSSALPEAFKKYAAKVSVN
                     TSSVQYENDDVMKPVWGDDYSICCCVSATQTGKEMQFFGARANLAKCLLYAINGGVDEK
                     SHEQCGPNYAPITSEYLTYDEVLPKYVQMLDWLAGLYVNVLNLIQYMHDKYYYEEAEMA
                     LIDTDVRRTFATGIAGFSHVIDSLSAIKYAKVKVVRDESGLATGFETEGDFPKYGNDDD
                     RADEIGVWLLKTFLEMIKKRHTYRNSEATTSILTITSNVVYGKYTGALPDGRAAFTPFA
                     PGATPSYGAEQNGLLASLNSVAKLPYHWALDGISNTQTINPEALGHSEDERVENLVQVL
                     DGYFDQGAHHLNVNVFGKEKLLDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVL
                     ARTCHGVL"
ORIGIN
        1 atacggcaca gcgctgcgtg tgccgtacta tttttggagg ttttgtatta tggctcgttt
       61 tactctgccc cgtgatctgt accatggcaa gggcgctctg gaagcgctga aatcctttac
      121 cggcaaaaag gcaatgatct gtgtcggcgg cggctccatg aagcgtttcg gcttcctgga
      181 tcgtgccgtg gagtacctga aggaagccgg catggaagtg gagctgttcg agggcatcga
      241 acccgatcct tccgtggaga ccgtcatgcg cggtgccgag gctatgctga agtttgagcc
      301 cgactggatc atcgccatgg gcggcggctc tcccatcgat gcagctaagg ccatgtggat
      361 caagtacgag taccccgaga tcacctttga ggagatgtgc aaggtgttcg gcatcccgcc
      421 gctgcgccgc aaggcacact tctgcgccat ttcttctacc tccggcactg ctaccgaggt
      481 gaccgccttc tccattatca ccgactacca gaagggcatc aagtacccca tcgcggactt
      541 cgagatcacc ccggacgtgg ccatcgtgga ccccgatctg gctgagacca tgcccaagaa
      601 gctggttgcc cacaccggta tggacgccat gacccacgct gtggaggctt acgtttccac
      661 tgcacactgc gactacaccg acccgctggc catcttcgcc atccgcatga tccagggcga
      721 tctggtggac agctacaatg gcgatatgtc caagcgcgac tccatgcaca acgcacagtg
      781 ccttgccggt atggctttct ccaacgcact gttgggcatc gtgcactcca tggcccacaa
      841 gaccggcgct gcctttgcag actacggcgc acacatcatc cacggcgcgg ccaacgccat
      901 gtacctgcct aaggtcatcg ccttcaacgc caaggacccc gaggccaaaa agcgctacgg
      961 cgtcatcgca gacgagatga agctgggcgg cgcaaacgat gacgaaaagg tcaagctgct
     1021 catcgagtac ctgcgcggca tgaacgacgc gctgaacatc ccccactgca tccagcacta
     1081 cggcccggac tcctacccca ccgagcaggg ctttgtgccg gagaatgtgt tcctggagcg
     1141 gctgcccgag atcgccaaga acgccatcgc cgacgcctgc accggctcca acccccgcca
     1201 gcccagtcag gaggagatgg agaagctgct caagtgctgc tactacgata ctgaggtgga
     1261 tttctgatcc gtcctttctt tattacgcct ccttccctat ggacaaaagc cccgcagagc
     1321 ataccgccct gcggggcttt tgccggtttc gcctgcattc tcccgtgaaa aaaggggttc
     1381 ggaaacgcct acggcgtttc cgaacccctt tcatactact aaggcgttac agcttgctca
     1441 gctggggacc ctgtgcggtg tccttgacag cccagcccag ttcggtcagc tgtgcgcgga
     1501 tggcatcagc ggtggcccag tccttggcct tcttggcagc agcacgcttc tccaccagcg
     1561 cggtcacctc ggcgggcact tcgtccttct tgcggttgta cagcagaccc agcacgccgg
     1621 tgatctcgtc aaaggcggcg gcagcggtct ccagtgcagc cttgctggaa gcagcagaca
     1681 gggtgttgat ctccttgacc agatcaaaca cggcagcaag cgcatccggg gtgttcaggt
     1741 cgtcgtccat ggcggtgcag aacttggtgc gtgcctcggc ggccttttcc ttcagggtct
     1801 cgtcggtgcc gaagtcggtc ttgctcaggg caaagtccag attctcgcgg caggtgtaca
     1861 ggcgctccag actgttcttg cagctctcga tgaggtctac ggtgtagttc aggggcatgc
     1921 ggtagccggc ggtcagcatg aagtagcgga tgggctcgta gccgtagagg tttgccacat
     1981 cccgcacggt aaagaagttg tgcaggctct tggacatctt ctgattgtcc acgttgatga
     2041 aaccgttgtg catccagtag cgggcaaact cgcagccgtt ggcgcactcg ctctgggcga
     2101 tctcgttttc gtggtggggg aagatcagat cctgaccgcc gcagtgcagg tcgatggtct
     2161 tgcccagatg ggtgcggctc atggcgctgc actcaatgtg ccagcccggg cggcccatgc
     2221 cgtaggggct ctcccatgcg ggctcaccgg gctttgcagc cttccagatg gcaaagtcgg
     2281 cggggtcttc cttcaaatcg tcctccatac ggctgcgcag ctcgcggttg ccgctctcca
     2341 gatcgtccag attcaggtgg ctcagcttgc cgtagccggg gtcgctcttc acccggaagt
     2401 acacatcgcc gttcttggca acataggcgt ggccagagtc gatcagatct ttgacgatat
     2461 ccaaaatctg ggggatatgc tcggtagcgc ggggctgcac ggtgggcttt tcgcagttca
     2521 gaccggcggc atccttcagg tactcggcct caaagcgctg tgccacctcc ttcatggagg
     2581 tgccctcttc ctgccccttc ttgatgagct tatcgtcgat atcggtgatg ttggaaacgt
     2641 agatcacctt gttgccccgg tattccagat agcggcgcag agtgtcgaac acgatcagcg
     2701 gacgtgcgtt gccgatgtgg atatagttgt acacggtagg gccgcagacg tagatgcggt
     2761 actcgccggg cacctgcggc acaaattctt ccttctgtcg ggtcagggtg ttgaagatct
     2821 gcatggtcaa ttctcctttt tgttccaaac agaagccgca aaacaaaacg cccccggagc
     2881 ggtgcacaag gcacaactcc gaaggcggtt ttaaaatcaa aatcgcggtt ccacttctgt
     2941 ttgtcgctca aagccggtaa cggcggcgta ccgcacggcg atacttcccc acggggttcc
     3001 cgccgcgtgc tgtccgggcg cactttgcgc agcggtctgc aaacaggctt ccagccagtg
     3061 gcctgttctc tctgcgcaga cggtgtgcgg cgctactctg cccggataaa cgcatttatc
     3121 cttttacccc tttattatag cggtgcggtg cagggaagtc aaggagggaa caaagaacga
     3181 ttcagaatgc ccgccgcgtt gctctgggca tcctcagcgg aagaattatt ttaaatttcg
     3241 ggatatcgga acgcctgcgg gcgttccgat atcccatttt tacccgtgaa aagggccccc
     3301 ggagcgtccg cagacgctcc gggggccaaa atcatagata ttctttccgc tgaaattgcg
     3361 cagagcgaag cggagcgcaa tcaaaacggt ggtgctttac ccgggatagc ggctcacttc
     3421 cagcagttct tctgcccgct ttacctgctc tgccgtgggc ggtactgcgt ccggcagggg
     3481 ataggggatg cccagcttct gccacttgaa cagccccaag gtgtggtagg gcagcacctc
     3541 cacccgctgc accgttttca ggctgcggat aaagtctgct gttttgcgca gcccttcctc
     3601 gtcatcggtc agcccgggca cgaggacgtg ccggatccac agcgggatgc cgaggtcgga
     3661 gatgtgccgc gccatggcaa ggatgttggc gttgtccttg ccggtgagct gccggtggcg
     3721 ctccgggtcg atctccttga ggtcaaggat caccagattg gtgttagcca tcaggcggtc
     3781 gaacgcggcc agataggccg ggtcgtccgg gcggaagggc tgcccggcgg tgtccagcgc
     3841 cgtgtgcacg cccttggcac gcgccagcgt gaagaactcg gtcagaaagt ccagctgccg
     3901 cagcggctcg ccgccgctga ccgtgatgcc gccctttttg ccccagtagt tgcggtagcg
     3961 gtatacccgc tggaacagcg cttctgccgt ccacgcctcg ccgccctctg cccatgtttc
     4021 ggggttgtgg cagtacttgc accgcagggc acacccctgc aaaaacacca caaagcgcac
     4081 gccggggccg tccaccgagc cgaaggattc cagcgagtgg acgtacccaa gggtcttaca
     4141 ggacgccatg gcaggtgcgt gccagcacgt ccagctgctg ctcgcgggtc aggtcgatga
     4201 acttgacagc gtagccggaa acgcggatgg tgaagttggc gtactcttcc ttctcagggt
     4261 gctccatggc gtccagcagc ttctccttgc caaagacatt cacgttcagg tggtgtgcgc
     4321 cctgatcgaa gtagccatcc agtacctgca ccagattctc cacgcgctcg tcctcgctgt
     4381 gacccagtgc ctcggggttg atggtctggg tgttggagat gccgtccagc gcccagtggt
     4441 agggcagctt tgccacggag ttcagcgagg ccagcagacc gttctgctct gcaccgtagc
     4501 tgggggtagc gccgggggca aacggggtga aggcggcgcg gccatccggc agagcgccgg
     4561 tgtacttgcc gtacaccacg ttggaggtga tggtcaggat ggaggtggta gcctcggagt
     4621 tgcggtaggt gtggcgcttc ttgatcatct caaggaaggt cttgagcagc cacacgccga
     4681 tctcgtcggc gcggtcgtca tcgttgccgt acttggggaa gtcgccctcg gtctcaaagc
     4741 cggtggcaag gccgctctcg tcgcgcacca ccttcacctt ggcgtacttg atggcgctca
     4801 gggagtcgat gacgtgggag aagcctgcaa tgccggtggc aaaggtgcgg cgcacatcgg
     4861 tgtcgatgag tgccatctcg gcttcctcgt agtagtactt gtcgtgcata tactggatga
     4921 ggttcagcac gttgacatac agcccggcca gccagtccag catctgcaca tacttgggca
     4981 gcacctcgtc ataggtcagg tactcgctgg tgataggggc atagttgggg ccgcactgct
     5041 cgtggctctt ctcatccacg ccgccgttga tggcgtacag caggcacttg gcaaggttgg
     5101 cgcgtgcgcc gaagaactgc atctccttgc cggtctgggt agcagacacg cagcagcaga
     5161 tggagtaatc gtcgccccag acgggcttca tcacatcgtc gttctcgtac tggacgctgc
     5221 tggtgttgac ggacaccttg gcggcgtact tcttaaaggc ctcgggcagg gcagaggagt
     5281 acagcacggt caggttgggc tccggtgcgg ggcccatgtt ctccagcgtg tgcaggaagc
     5341 ggtagcagtt cttggtgacc a
//
