LOCUS       c00217_gnlPro..        15410 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LANLAPFM_217.
ACCESSION   c00217_gnlPro..
VERSION     c00217_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:37:01
            Original ID  :: gnl|Prokka|LANLAPFM_217
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 819
            Orig. end    :: 16229
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..15410
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[10819:16063]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      10001..15244
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..15410
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..15410
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             complement(18..314)
                     /locus_tag="ctg217_2"
                     /transl_table=11
                     /translation="MKDNRNRYNEEIEKYQSMDQFQVGIAVFDPGTTETQFYDNVRSVC
                     EMMNNNISISVGVTWAEHGKEFNEKIMEADWRMYEQKNAYHQQQQAAQQELLQ"
     CDS             complement(317..586)
                     /locus_tag="ctg217_3"
                     /transl_table=11
                     /translation="MCRADLTERAVEEKDMLVLAKEEENIVVNKPALSQIRSICELATL
                     ECYYHNVEKSAKTKGTGLAHVGEKEKNSGSNTPELLRLKSTCRM"
     CDS             complement(802..2463)
                     /locus_tag="ctg217_4"
                     /transl_table=11
                     /translation="VLTNGETFSYDKNEIESYVVTGLKYVPVKVKTEDYEAFKAAYTVV
                     ENGSTLSGGFSEENLKNYTDLVAEVTENTNGLKTVTQNEDGSFSFAARVNNGTDSGIKD
                     AALKTAENITTTVKEANGSYGEFLRVDLTGEGYGALGADMQAVEWTYYGSDSTYTDPLQ
                     SYGTKFASDNWMHKAQGIQLGLTDSLRCKLPAGTDGTGYWTITVYALGYNDYTVKFKVT
                     DANIVKDEEETVDTTALEAAIKSAENLTESDYTAASWSDLCVELKEAKDELAAPHTQST
                     VDEATEHLNAAIKALVKAETKEETKTDVTKLNAVIEKAEALKQSDYTAESWKNLQTALD
                     AAKKLTDATAEQTVVDQAASDLETAILALVKADTENTGTTDKKKKPAVGTVKTVGQIKY
                     KVTGKNTVTVNKYAKKNITKASIPATVKINGYTFKVTAIADSAFSGCSKLTKVTVGSNV
                     KAIGNKSFYKCTKLTTFTASSTGLNKIGKEAFSGDKKLANITLKTTKLKKSGVGKDAFK
                     NIKKNATFKVPAKKVSDYKAIFKSKGAGKNIKIKKL"
     CDS             complement(2457..2804)
                     /locus_tag="ctg217_5"
                     /transl_table=11
                     /translation="MKRKNVSARILTGVLAFSMVAATGTSSMTVFAAEESAVEAEQITG
                     TEVKSETGETQEYQYAYAALSLTWVHLIPYREQPPITDCTGEVISVRQKLQKKMVQSSK
                     WHIIQEQPPVC"
     CDS             complement(2953..3732)
                     /locus_tag="ctg217_6"
                     /transl_table=11
                     /translation="LESTKLKNIVEIIQKNCISKERKTNGAVFWKKHKKQIPAWILAGI
                     QLLFFVFFPSAFTAAFSGVKYIAGQMGTGAQIEITSFVLSLIIFCASTVVFGRFFCGFA
                     CAFGSLSDWVHAAYVWACKKLKKKPVTLGMELTAAVRGMSFPCFMQEILHWKDILRESS
                     CSFSSCEACACRSVFSAVFCARWERYFHFFQYCHILRSEEKGRTVSLAVQYARKNVPRI
                     SDCRNWGRLRREATVLCVRNVPASVRKEISTMVSLEN"
     CDS             complement(3747..4184)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FMN_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg217_7"
                     /sec_met_domain="FMN_bind (E-value: 4.4e-17, bitscore:
                     52.9, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDFLIRSFCLAAVILILAGYNQVLKDRSKDEEITKLEVQVTKLQ
                     QEKEKTVDIKGTYPDGRWEGGAKGFGGMITVLVTVENGTISEIEITSADGEDKAYLSMA
                     EDIIPKIIEAQSADVDTVSGATFSSTGIRDAVSEALKQAEQ"
     CDS             complement(4198..5901)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FMN_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg217_8"
                     /sec_met_domain="FMN_bind (E-value: 5.7e-18, bitscore:
                     55.7, seeds: 891, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNISGIVPVILAAVVGIGAVVSLSDYTAPVYGSESEDELTETET
                     IIESAGMESVEETEVENTEAAVVLDGAFDLADGTYEGSANGFSGKIKVSVVIKNQTIRS
                     INILSNSDDEAFFNRAKEGVTASILAKQSTDVDTVSGATYSSRGIINAVKDALSSSDGE
                     EQTVMAKGDFALNDGYYEGTGNGFAGPVKLFIEIKDKSIIGIYIVKTSDDAGFFNRAKE
                     GVTASILEKQSLSVDTVSGATYSSKGIIEAVSNAMEAAKKAKKGETDTETEDDTEKPDT
                     SKASSYYGVVVCNPDESGAFTAYNLHVKVMFQNGKIIAVSDVYGDGDSQNDTYITKAVS
                     GSSKHPGVVTQIVNKNGIDGVDTVSGATCTSKAVLHAVDVVVKAAAKDGNYTFSGSQSG
                     DDDTKNSEDTEKDSEDTENNSENTEKNSENTENNSEDTENRGENTESGGDTEKNDTENT
                     ENSGETTLIYKNGDYKVSVVCIPDEDEDFDEYKLTLTLVIENDQIKEIKDISGDGGKSN
                     SFYIKAASEGVVSQILQKNSTEGIDTVSSATCSSKAIIEACQKALEQAKN"
     CDS             complement(5928..6968)
                     /locus_tag="ctg217_9"
                     /transl_table=11
                     /translation="MSEGKVKRKIIFLIFAAVMVVAGGCGTDGGDTEASRDLFAMDTYM
                     TVTAYGKGATEAVDQAADEISRLDQLLSTGNEDSEIYKINQSGDGILSEETAYLVERSL
                     DLYEATDGAFDIAIYPVMKAWGFTDDNFRVPDADELKDLLTLTDASDISYDKETSQIAF
                     KKDGMQIDFGGIAKGYTSARIMDIFRACGIKSGLVNLGGNVQALGTKKDGSSWRVAVQD
                     PQDTDQYLGVLSIQDKAVITSGGYERYFEQDGRTYHHIIDPKTGYPVENGLISVSIVTA
                     DGTLADGLSTSVFIMGKEAATEYWRNHSDEFDMILMTDDREIYVTKGIADSFESEMDTK
                     IIEKKV"
     CDS             complement(7164..7847)
                     /locus_tag="ctg217_10"
                     /transl_table=11
                     /translation="LRNEHASVILNNVVKLHITRKEEETMTQKKFSTKYLVEMALLVAI
                     ILIMAFTPIGYIRTAGLEITLIVVPVAVGAVTLGPAAGAILGGVFGVTSFIQCFGMSPF
                     GAALLGINGFLTFLVCVPTRILMGWLTGLIYKGLRKTKLPSGVSVTISNLCCPLLNTTF
                     FMGMLVIGFYNTEYIQSFVSALGAKNALLFILAFVGVNGLVEAIVCFVVGTAISAALKK
                     ALKYN"
     CDS             complement(7918..8526)
                     /locus_tag="ctg217_11"
                     /transl_table=11
                     /translation="MNKKKFSTKFLVEMALLVAIILIMAFTPLGYIKTAGIEITLIVVP
                     VAVGAVTLGPAAGAILGGVFGLASFLRCFGLNAFGAMLLSINPFLAFLLYVPTRILTGW
                     LTGLIYQGIRKTKIPSNVSITIANLCCPLLNTTFFMSMLVFGFYQTEYIQSFVQTLGVS
                     NPFLFVIAFVGINGLVEAVVCFIVGTAISAALKKALKYS"
     CDS             8829..9443
                     /locus_tag="ctg217_12"
                     /transl_table=11
                     /translation="MIQKKNVLNTVPIICLGALLLGAAYGQLHTPMTPPPITALASDIL
                     PTTGNVRALVVVVDFADAKYDTDRRLSDEEISSYLFGTDNTEFYPCESLTSYFDRASYG
                     SLHMTGNVFHYTAKGTIASYEKTNDGYETLVREVLSGLNDTIDYTDYDSDGDGYIDALC
                     LSVPSGGNADFWYGCTASWWASTLPDLDGEKLRKLSDHTAH"
     CDS             9469..9828
                     /locus_tag="ctg217_13"
                     /transl_table=11
                     /translation="MILDYITPNGNYDGLFSSGGLRIYHVDATLANDEYVTPYLTADNY
                     SPVYDSTNNGRRILRLVNNGNGSYRDGDVIGGTATGFGWYDNNGQLTIDTGYTITVDSV
                     SEDQKNCTITILTNN"
     CDS             complement(10001..11047)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 161.3;
                     E-value: 7.7e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg217_14"
                     /sec_met_domain="ETF (E-value: 2.3e-50, bitscore: 161.3,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 1e-38, bitscore:
                     121.7, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALEEYKGVFVFAQQVDNVLDGVAFELLGKGKDLAKDLGTDVTAV
                     LIGSGVKGLADQLAEYGADKVIVVDDPELKDYRTEPYAHALASVINEFKPEIMLVGATA
                     IGRDLGPTVSARVKTGLTADCTSLEIGDFPLVAAPGKESEQKHNQLLMTRPAFGGNTIA
                     TIACPDNRPQMATVRPGVMQKIAPISGAKANVVEYNPGFTPNNRYVEILNIVKAVKSTA
                     NIMEAKILVSGGRGVGSKENFKLLEDLAEVLGGTVSCSRAVVENGWLPVDLQVGQTGKT
                     VRPQIYFAIGISGAIQHVAGMEDSDLIIAINKDEDAPIFDVADYGLVGDLNKIVPALTT
                     ALKAELGK"
     CDS             complement(11074..11856)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 158.5;
                     E-value: 5.5e-49)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg217_15"
                     /sec_met_domain="ETF (E-value: 1.7e-49, bitscore: 158.5,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVVCIKQVPDTKGGVKFNPDGTLDRAAMLAIMNPDDKAGLEAA
                     LRIKDETGAKVTVLTMGLPKADAVLREALAMGADEAVLVTDRVLGGADTWATSTTIAGA
                     LRNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLPVISYAADIKVEGDSVVVKRQYEDRY
                     HELKVKMPCLVTALSELNEPRYMTPGGIFDAFDKEVTVWGRADLKDVDDSDLGLKGSPT
                     KIAKASDKVAKGAGEKVNLDPAESVAYLIGKFKEKHII"
     CDS             complement(11913..13067)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg217_16"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1e-52, bitscore:
                     168.6, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 3.9e-31, bitscore:
                     97.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 7.4e-40, bitscore:
                     126.6, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLDKKHEMARSLFKEFAETEVKPLAQETDETEAFPAETVKKM
                     QKYGFMGIPVPKEYGGQGCDPLTYVMCVEELSKVCATTGVVVSAHTSLCIDPIMTYGTE
                     EQKQKYVKPLATGEKLGAFALTEPGAGTDAQGAQTKAVLDGDEWVLNGSKCFITNGKVA
                     DVYIVIAITSITEDKRGRKKKNFSAFIVDKGAPGFSFGTKEKKMGIRGSSTYELIFEDC
                     RIPKENLLGPEGKGFPIAMHTLDGGRIGIAAQALGIAEGALDRAIAYTKERKQFGRSIA
                     QQQNTQFKLADMAARIEAAQLLVYKAAMAKATQKVYSVEAAKAKLFAAETAMAVTTEVV
                     QLFGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGALLK"
     CDS             complement(13097..13969)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg217_17"
                     /sec_met_domain="HCDH (E-value: 3.2e-35, bitscore: 111.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 2.3e-63, bitscore: 203.6,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGVIGAGTMGQGIAKAFAQVEGYEVALCDIKQEWAEGGKEKIK
                     KGYARLVEKGKMTQEAVDGILAKITPGLKEDLCKDCDLIVEAAFENMEVKKTTFKELDE
                     IAKPDCIFASNTSSLSITEIGNGLNRPMVGMHFFNPADRMKLIEVIAGVNTPDETVSAI
                     KKISEEIGKTPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDAAMKLGANHPM
                     GPLELGDFIGLDICLAIMDVLYKETGDSKYRACPLIRKMVRGGNLGAKSGKGFYVYNAD
                     RTKTPVDAQ"
     CDS             complement(14063..15244)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg217_18"
                     /sec_met_domain="Thiolase_N (E-value: 8.6e-98, bitscore:
                     317.1, seeds: 30, tool: rule-based-clusters)"
                     /sec_met_domain="Thiolase_C (E-value: 2.7e-51, bitscore:
                     162.8, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKKVVLAGACRTAIGKMGGALSNTPAADLGAIVIKEALRRANVK
                     PEMVDEVKMGCVIQAAQGQNVARQAAIKAGLPIEVPAITINVVCGSGLKCVNDAATMIM
                     AGEADIVVAGGMENMSMAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMITAE
                     NVCDKYGITREELDEFSANSQQKCEKAIAEGKFDDEIVPVPVKVKKEIVDFVKDEGPRA
                     GTTAESLAKLKCCSGKEGGLVTAGNASGINDGAAAIVVMSEEKAKELGVTPMATWVAGA
                     LGGVEPEIMGVGPVASTRKVLAKTGLSIDDMDLIEANEAFAAQSIAVARDLKFDMSKVN
                     VNGGAIALGHPVGASGCRILVTLLHEMQKRDAKRGLATLCIGGGMGCSTIVER"
     CDS             complement(15259..15408)
                     /locus_tag="ctg217_19"
                     /transl_table=11
                     /translation="FKMKEKKINFNRKFEFIFLKKIVKKLTAFFGNFKEKETNSLQKQS
                     FVLN"
ORIGIN
        1 actattcttt caaacattta ttgcagcagt tcctgttgtg cagcctgctg ctgttgatga
       61 tatgcatttt tctgttcata catccgccag tcagcttcca tgatcttctc attaaattct
      121 tttccgtgct ctgcccaggt aactcccact gaaatgctaa tattgttgtt catcatttca
      181 cagacagatc ttacattatc ataaaattgc gtttctgtcg tacccggatc aaatactgca
      241 atgccaacct gaaactgatc catactctga tatttttcta tttcctcatt gtatctgtta
      301 cgattatctt tcatatttac atccgacatg tcgatttcag tcttagcaac tccggtgtat
      361 tcgatccaga atttttttct ttttctccca catgtgccaa tccggtacct tttgttttcg
      421 cggacttttc cacattgtga taataacatt ctagtgttgc aagttcacag atcgaccgga
      481 tctgtgacag ggcaggttta ttgacaacaa tattttcctc ttcctttgcc agcacaagca
      541 tatccttctc ttctactgca cgctctgtca gatccgctct gcacatttca tacgcttcct
      601 gcgatatcgt ttctgtttca tatttttatt ttcaaaaata taatccatag aaccaaaacc
      661 taccgccatg ttctgtatct cactctccgg aagtgtgagg gtgattgttt ttgtttcctc
      721 ctcaaaggtt caattacaca ttccattcta caataaggtc tcttcacccc ctgtttttta
      781 acaaaagaat attttttcac cctacagttt ctttatctta atattttttc cagcaccctt
      841 tgacttaaaa atagctttat aatcagaaac tttttttgcc ggtactttaa aggttgcatt
      901 tttcttaatg ttcttgaatg catcctttcc aacacctgat tttttcagtt ttgttgtctt
      961 taatgtgata tttgcaagtt ttttatcacc ggaaaacgct tcctttccaa tcttgtttaa
     1021 tccggtagaa gatgccgtaa atgtggtaag ttttgtacat ttataaaatg atttatttcc
     1081 gattgccttt acattagaac caaccgtaac tttcgtaagt ttactgcatc cggaaaatgc
     1141 gctgtctgca attgcagtta ccttaaatgt atatccgttg attttaaccg ttgcaggaat
     1201 agatgccttt gtaatgtttt tctttgcata tttatttaca gttactgtat tttttccggt
     1261 tactttatac ttgatctgtc caactgtttt caccgttcca actgccggtt ttttcttttt
     1321 gtctgtagtt ccggtatttt ccgtatcagc ttttaccagc gccaaaatcg ctgtttccag
     1381 atcacttgct gcctgatcta ctacagtctg ttctgctgtt gcatctgtaa gtttctttgc
     1441 agcatcgagc gcagtctgta aatttttcca gctctctgct gtgtaatcac tctgctttaa
     1501 tgcctccgct ttctcgatta cagcgttaag ttttgtaaca tctgtttttg tttcttcctt
     1561 tgtctctgcc tttaccagtg ctttgattgc agcattcaga tgctctgttg cctcatctac
     1621 agtagactgc gtgtgcggag ccgcaagctc atcttttgcc tctttcaatt caacgcaaag
     1681 atcagaccag cttgctgccg tataatcact ctcagttaag ttctctgcac ttttaattgc
     1741 agcttcaagt gccgtcgtat ccaccgtttc ctcttcatcc ttaacaatat ttgcatcggt
     1801 taccttaaat tttaccgtgt aatcattgta accaagtgca tataccgtga ttgtccagta
     1861 tccggttccg tctgttcccg ccggcagttt acaacgcagt gaatctgtaa gaccaagctg
     1921 gattccctgt gctttgtgca tccagttatc tgatgcaaac ttggttccat aagattgaag
     1981 tggatcggta taagtactgt cggatccata ataagtccac tctactgcct gcatatcagc
     2041 accaagtgca ccgtagccct ccccagtcag atcgacacga agaaattctc cgtaagaacc
     2101 gtttgcctct tttaccgttg ttgtaatatt ctctgctgtt tttaatgcag cgtccttgat
     2161 tccggaatcc gttccattgt ttactctcgc agcaaacgaa aaacttccgt cctcattttg
     2221 ggtaactgtt tttagaccat tggtgttttc tgttacttct gcaaccagat ccgtatagtt
     2281 tttcagattt tcctccgaaa atccaccgga taaagtactg ccattttcca ctacggtata
     2341 tgctgcttta aaagcttcat aatcttcagt ttttactttg actggaacat atttaagtcc
     2401 tgttaccaca tagctttcaa tctcattttt atcataggaa aaagtctctc cgttcgtcag
     2461 cacaccggtg gctgctcctg tataatatgc cactttgagc tttgtaccat ctttttttgt
     2521 aatttctgac gtacactgat aacttcccct gtgcagtccg tgattggtgg ttgctctcga
     2581 tacggtatca aatgcaccca tgtcaagctc agcgctgcat aagcatactg atactcctgc
     2641 gtttctcccg tttcactctt cacctcggtt ccagtgatct gctctgcttc cactgcactt
     2701 tcttctgcgg caaaaaccgt catggacgat gtccctgttg ctgccaccat ggaaaaagcc
     2761 aatactccgg tcaggatccg ggcagataca ttttttcttt tcattttttt aatccttcat
     2821 tttgctttat ctatcaaaag ttagtctttg ttaactctca atcaaaaaat tcacactatc
     2881 agattccgat ccacaggtat aagaaaagca gaaataccgc ccttatggca gtaaaccaca
     2941 attcattccc tttcaatttt ccagagacac catagtggat atttcctttc ggacagatgc
     3001 cggtacattt ctgacacata aaacagtcgc ctctcgtctc aatcgccccc agttccggca
     3061 gtccgatatc cgagggacat tttttcgtgc atactgcaca gccagagata cagttctccc
     3121 tttctcttct gagcgcaaaa tatggcagta ctggaagaag tgaaaatatc gctcccatcg
     3181 ggcacaaaat acggcagaaa aaacgctcct gcacgcacat gcctcacagg atgagaatga
     3241 gcaggacgac tcccgcaaaa tatccttcca atgcaaaatt tcctgcatga agcatggaaa
     3301 agacatccca cggactgctg ccgtaagttc catgccaagc gtgaccggtt tcttttttaa
     3361 ttttttacag gcccagacat aagctgcatg cacccagtcc gacagacttc caaatgcaca
     3421 ggcaaatcca cagaaaaaac ggccgaacac tacggtagat gcacagaata tgataagtga
     3481 cagcacaaat gaagtgattt caatctgtgc acccgttccc atctgtccgg caatatattt
     3541 cacaccggaa aatgcagccg tgaacgctga cggaaaaaat acaaaaaaca ataactgtat
     3601 tcccgcaagg atccatgccg gaatctgctt tttatgcttc ttccagaaaa cagctccatt
     3661 tgtctttcgt tcctttgata tacaattttt ttgaattatt tcaactatat tttttaactt
     3721 cgtactttcc aaatttttct tcactcttac tgttctgcct gtttcagtgc ctcgctgaca
     3781 gcatccctga tcccggtaga actgaacgtt gcaccgctta cggtatccac atctgcggac
     3841 tgcgcctcta tgatctttgg aataatatct tccgccatgg ataaataggc tttatcctcg
     3901 ccgtctgcgg aagttatctc aatttcgctg attgttccat tttccacggt cacaagcacg
     3961 gtgatcattc cgccaaaacc ttttgccccg ccttcccatc tgccgtccgg ataagttcct
     4021 tttatatcga cagttttttc tttttcctgc tgcagttttg tcacctgcac ctctaacttt
     4081 gtgatctctt catccttgct tctgtccttt aatacctgat tgtaacctgc cagtatcaat
     4141 atgaccgcag ccagacaaaa tgaccggatc aaaaaatctt tcatctgcta ttctccctta
     4201 attttttgcc tgctccaatg ccttttgaca ggcttcaatg attgcttttg aggaacaggt
     4261 tgcagacgat accgtatcga tcccttctgt actgttcttt tgtaatatct gactgacaac
     4321 accttctgac gcggctttta tatagaatga attgctcttt cctccgtctc cggaaatatc
     4381 tttgatctcc ttgatctgat cgttttcgat cacaagcgtg agcgtcagtt tatattcatc
     4441 aaaatcttca tcctcatccg gaatgcagac aacactgacc ttataatcac catttttata
     4501 gatcagcgtt gtctcgccac tattctcggt attttccgta tcattttttt cagtatctcc
     4561 gccactctca gtattttcac ctctattttc cgtatcctcg ctgttatttt ccgtattttc
     4621 gctgttcttt tccgtatttt cgctgttgtt ttccgtatcc tcactgtctt tttccgtgtc
     4681 ttcgctgttc tttgtatcgt catcaccgct ctggctgcca gagaacgtgt agtttccatc
     4741 ttttgccgca gcttttacca ccacatccac ggcatgcagt actgcctttg atgtacaggt
     4801 tgcaccggag accgtatcca ccccgtcaat tccgttttta tttacgatct gtgttacaac
     4861 ccccggatgc ttactactgc cggaaaccgc ctttgtaata taagtatcat tctgggaatc
     4921 tccatccccg tacacatcgg aaactgcaat gatttttcca ttctgaaaca tcacttttac
     4981 atgcaggttg tacgccgtaa aagcaccgct ctcatccggg ttacagacaa cgactccata
     5041 ataggagctc gcttttgatg tgtccggctt ttcggtatca tcttctgttt cggtatccgt
     5101 ctcccccttt ttcgcttttt ttgcagcctc catcgcattt gatactgcct caatgattcc
     5161 tttggaactg tatgttgcac cggaaaccgt atctacagac aggctctgct tttctaaaat
     5221 acttgccgtc acgccctcct ttgcacggtt aaaaaatccc gcatcatctg atgtttttac
     5281 aatataaatc ccaatgatcg acttgtcttt gatctcgata aacaatttga ccggtccggc
     5341 aaatccattt ccagttcctt cataatagcc atcattcaac gcaaaatctc cctttgccat
     5401 aaccgtttgt tcttccccgt cactggaaga taacgcatct tttactgcat ttatgattcc
     5461 tcttgaactg tatgttgcac cggaaaccgt atccacatcc gtactctgct ttgcaaggat
     5521 gctcgctgtc acaccttctt ttgcacgatt gaaaaaggct tcatcatctg aattactcaa
     5581 aatgttgatc gaccggatcg tctgattttt tatcacaact gaaactttga tcttaccgga
     5641 aaatccgttc gcactgcctt cataagttcc gtctgcaaga tcaaacgcac cgtctaaaac
     5701 aacagccgct tccgtatttt caacttccgt ctcttctacc gactccatac cggcactttc
     5761 gataatagtt tccgtctctg tcagttcatc ttctgactct gaaccataaa ccggtgctgt
     5821 ataatcactc agggaaacca ctgcaccaat tcccaccacc gctgccagaa tgacgggtac
     5881 aattcctgaa atatttttca tacttgtctc cataccaaat cattcgttta tacttttttt
     5941 tcaatgatct tcgtatccat ttccgactca aaactgtctg cgattccctt tgtgacatag
     6001 atttccctgt catccgtcat cagaatcatg tcaaattcat cactgtgatt tctccaatat
     6061 tccgttgcgg cttctttccc cataataaaa acggatgtag aaagtccgtc cgcaagcgtt
     6121 ccatctgctg tgacaatgga aacggaaatt aacccgttct ccaccggata accggttttg
     6181 ggatctatga tatgatgata tgtcctgccg tcctgctcaa aataacgttc ataaccgccg
     6241 gaagtgatca ccgccttatc ctgtatggaa agcaccccaa gatactgatc tgtatcctgt
     6301 gggtcctgca ctgccacgcg ccagctgctt ccatccttct ttgttccaag tgcctgcaca
     6361 tttccaccaa gattgaccag tccgctcttg atcccgcagg cacgaaaaat atccatgata
     6421 cgtgcagaag tataaccttt cgcgattcct ccaaagtcga tctgcattcc gtccttttta
     6481 aaagcgatct gcgatgtctc tttatcataa gaaatatccg atgcatctgt cagcgtgagc
     6541 agatccttaa gttcgtctgc gtccggcacc ctgaaattgt catctgtaaa tccccatgct
     6601 ttcataaccg gataaatcgc aatatcaaat gcaccatctg tagcttcata cagatccagt
     6661 gagcgttcca ccagatatgc tgtctcctcc gagagtattc catctccgct ctgattgatt
     6721 ttatatatct cactgtcctc atttcccgta gacaacaatt gatccagccg gctgatctca
     6781 tctgccgcct gatccacggc ctctgtggct cctttgccat aagcagttac cgtcatatat
     6841 gtgtccattg caaagagatc tctgctcgcc tcggtatctc ccccatcggt accacagcca
     6901 cctgccacaa ccatgactgc tgcaaaaatc agaaatataa tttttctttt tactttacct
     6961 tcgctcattt tccctctaac tttggttagt attttctaac cattttagct tacaatgcac
     7021 cagacgcaaa tgtcaatctg cacttttgag actttttctc attttgatct ccatttccca
     7081 tgttaccgca aaaaaatccc ctcacataca gacaacaacc tgcatataag aggattcctc
     7141 aattatatta tatttcattc aatttaatta tatttcaatg cctttttgag agctgctgaa
     7201 atcgccgttc ccacaacaaa gcagacaatt gcctcaacta gtccgttgac acctacaaat
     7261 gctaaaatga acagcaatgc attttttgca ccaagagctg atacaaatga ctggatgtat
     7321 tccgtattgt aaaatccaat cactaacatt cccataaaaa atgtggtgtt cagcagcgga
     7381 caacaaaggt tgctgatcgt aacagataca cctgacggca gctttgtttt acgaagtcct
     7441 ttatagatca gacctgtcag ccatcccatt aaaatacggg taggtacgca tactaaaaat
     7501 gttaaaaatc cgttgattcc aagcagtgca gcaccaaatg ggctcatgcc gaagcactgg
     7561 atgaaacttg taacaccgaa cacgccgcct aagattgcgc ctgccgccgg tcctaacgta
     7621 acagctccta ccgctaccgg aacgacgatc agcgtgatct caagaccagc tgttctgata
     7681 tatccgatcg gggtaaaagc catgatgaga atgatagcaa ctaaaagtgc catctccaca
     7741 agatactttg tggaaaactt tttctgagtc atagtctctt cctcttttct tgtaatatgt
     7801 aattttacaa cgttattaag tataacactt gcatgctcat tccgcaatcc cgcatccata
     7861 tttacttcgt aatgtcatgt ttttattttc atccaatttt taccatattc catccaatta
     7921 actatatttc aatgctttct tcaaagccgc ggaaattgcg gttccgacga taaaacatac
     7981 caccgcctca accagtccgt tgattccgac aaatgctatc acaaacaaaa atggattgct
     8041 gacaccaagc gtctgaacaa acgactggat gtactcggtc tggtaaaacc caaataccag
     8101 catgctcata aaaaacgtgg tatttaaaag cggacagcac agattcgcga ttgtgattga
     8161 tacattcgat ggtatttttg ttttgcggat tccctgataa atcagtcctg tcagccagcc
     8221 cgtcaggata cgggtcggta catacagcaa aaatgccaga aacggattga tgctaagaag
     8281 catcgcacca aatgcattca gtccgaagca gcgcaaaaaa ctggcaagtc caaatacacc
     8341 tcctaaaatt gcaccagccg caggccctaa agtaacagct cctaccgcca ccggaacaac
     8401 gatcagtgta atctcaatac ctgccgtctt aatgtatccg agtggggtaa aagccatgat
     8461 aagaatgatg gcaactaaaa gtgccatttc cacaagaaac tttgtggaga actttttctt
     8521 gttcatagtc ccttcctctt ttctcgtttt tatgtaattt tatacgaaac acaggacgag
     8581 ccatttgtgg atcagtataa atttccggca tcctgtatct ctgcaacgtt actaagtata
     8641 acatctggca gaattttccg caatcttaga ttgcgttaat cctaaaatcc taaaattgtg
     8701 ccattctcag aattgcacta ccactttttt atatttttat tgtttcctta attatcaata
     8761 aatgccattg actttctgct ttttgcgtgc aaagctaacc atatatttat aagaaaagag
     8821 tttttaccat gatacagaaa aaaaatgtac ttaacaccgt tccgattatc tgccttggtg
     8881 ccctgctttt gggggcggca tacgggcagc ttcacacgcc aatgacgccg ccaccgatta
     8941 cagctctcgc ctcagatatt ctgccgacga caggcaatgt ccgagcactt gttgttgtgg
     9001 tcgattttgc ggatgcgaaa tatgatacag acagacgcct gtccgatgaa gaaatttcct
     9061 cctatctgtt tggcacagac aatactgaat tttatccctg tgagagcctg actagttatt
     9121 ttgaccgtgc ttcatacgga tcactgcata tgaccggaaa tgtatttcat tacacagcga
     9181 aaggtacgat cgcatcctac gaaaaaacga acgatggata cgaaacgctg gtgcgtgagg
     9241 ttctttccgg tttaaatgat acgatcgact ataccgatta tgattctgac ggcgatggct
     9301 atatcgacgc tctctgcctt tctgttccaa gcggaggcaa tgctgatttc tggtatggct
     9361 gtacggcatc ctggtgggct tccaccctgc cggatttaga tggcgaaaaa ttaaggaagc
     9421 tgtctgatca taccgcgcac tgatgcttcc agttattttt cagaatacat gatccttgac
     9481 tatatcaccc cgaatggtaa ttatgacggt ctgttttctt ccggcggact tcgtatttat
     9541 cacgtcgatg ccacccttgc aaacgacgaa tacgttaccc cctatctgac cgctgataac
     9601 tacagtcctg tctacgattc caccaataac ggcaggcgga tcttaagact ggtcaataac
     9661 ggaaatggct cttaccggga cggtgacgtg atcggaggca ctgccacagg ctttggctgg
     9721 tatgacaaca acggacagtt gaccattgat accgggtata cgatcacggt cgactccgta
     9781 tctgaggatc agaaaaactg taccataacg attttgacaa acaactaagt atattttccc
     9841 tcaactcctg accgagagcc tcaaatttcc tccaactcca aacaagaagc ctcaaatttt
     9901 ctccagctcc cagcaaaaag cccgtccttc ctgtcagctt cctcacagaa gccgcacacg
     9961 aaagacgggc tttctatctt acaggttatt cacctatgta ttatttgcct aactcagctt
    10021 ttaatgctgt tgttaaagca ggaacgatct tattcagatc accaaccaga ccgtaatcag
    10081 ctacgtcgaa gattggagca tcttcatctt tgttgattgc gatgatcagg tcagaatctt
    10141 ccataccagc tacgtgctgg attgctccgg aaataccgat tgcaaagtag atctgaggac
    10201 gaacagtttt accagtctga cctacctgta aatcaactgg taaccagcca ttctctacaa
    10261 ctgcacggga gcagcttaca gtaccgccaa gtacctctgc aagatcttcc aaaagtttga
    10321 agttctcttt ggaaccaact ccacgaccac cggaaacaag gatctttgct tccataatgt
    10381 ttgctgtgct cttaacagct tttacgatgt ttaagatctc aacatatctg ttgtttggtg
    10441 taaatcccgg gttgtactct acaacgtttg cttttgcacc gctgatcgga gcgatcttct
    10501 gcataacacc tggacgtaca gttgccatct gaggacggtt gtccgggcag gcaattgttg
    10561 cgatggtgtt accaccaaat gcaggacgtg tcattaataa ctggttgtgt ttctgctctg
    10621 attctttacc aggtgcagca accagtggga aatcaccgat ctcaagagat gtacagtctg
    10681 ctgtcagacc tgtttttact cttgcagaaa cagtaggtcc taagtcacgg ccgatagcag
    10741 ttgcaccaac taacatgatt tcaggtttga actcgttaat tacagatgct aatgcatgag
    10801 catacggctc tgttctgtag tcttttaatt caggatcatc tactacgata actttgtcag
    10861 caccatactc agctaactga tctgctaatc cttttacacc agaaccaatt aatacagcag
    10921 ttacgtctgt acctaaatct tttgctaagt ctttgccttt tccaagtaac tcaaatgcaa
    10981 cgccatctaa tacgttatct acctgctgtg caaagacaaa tactccttta tattcttcta
    11041 atgccatgtt attcaccact tttccttttt ttattagata atgtgtttct ctttgaattt
    11101 accgatcaaa tatgctacgg attctgccgg atcaaggttt actttctcac cagcaccctt
    11161 tgcaacttta tcagatgctt ttgcaatctt agtaggagat ccctttaaac caagatcaga
    11221 atcgtcaaca tcctttaagt ctgctctgcc ccaaacggtt acttctttgt caaatgcatc
    11281 gaagattccg cccggtgtca tgtaacgcgg ctcatttaat tcagaaagag cagttaccag
    11341 acaaggcatt tttactttta actcatgata tctgtcttcg tactgacgtt ttacaacaac
    11401 agaatcaccc tctactttga tgtctgctgc gtaagagata accggaagtc caagatgctc
    11461 agcgatctga ggaccaacct gtgcagtatc accatcgata gcctgacgtc ctgtgatgat
    11521 caggtcatag tctaagttac gaagtgcacc agcgattgtg gtagatgttg cccatgtatc
    11581 agctccacct aatacgcggt ctgttacaag aactgcttca tcagcaccca ttgctaatgc
    11641 ttcacgaaga actgcatcag cctttggaag tcccatggta agaactgtaa cttttgctcc
    11701 tgtctcatct ttgattctaa gtgctgcttc aagaccagct ttatcatctg ggttcatgat
    11761 agcaagcatt gctgctctgt caagcgttcc atccgggttg aacttaacgc cgccctttgt
    11821 atcaggaacc tgtttaatac atactacgat attcatgtat ttttcactcc ttatgtttta
    11881 tatgtttaaa ttttacgcgg attgattcga aattacttta acagtgcacc ggagattacc
    11941 atacgctgga cttcgcttgt accctcgtag atctcagtga tcttagcatc acgcatcata
    12001 cgctctacat cgtactctct gatatatccg tatccaccga ataactgaac aacctcagtt
    12061 gtaacagcca ttgcagtctc tgctgcgaat aatttcgctt ttgcagcttc tacagaatat
    12121 actttctgag tagcttttgc cattgcagct ttgtaaacta aaagctgtgc agcttcgatt
    12181 cttgcagcca tatcagcaag tttgaactgt gtattctgct gctgtgcgat tgaacgtcca
    12241 aactgttttc tctcttttgt ataagcgata gctctgtcta atgcgccctc agcgatacca
    12301 agtgcctgag cagcaatacc gatacgtcca ccatcaagtg tgtgcattgc gatcgggaaa
    12361 ccttttcctt ccggtcctaa cagattctct ttcgggattc tgcagtcttc gaagatcagc
    12421 tcataagtag atgatccacg gatacccatc ttcttctctt ttgttccgaa tgagaagcct
    12481 ggagctccct tatcaacgat gaatgcagag aagtttttct tctttctgcc tctcttatcc
    12541 tctgtgatgc ttgtgatagc gattacaata taaacatctg caactttacc attggtaatg
    12601 aagcattttg atccatttaa tacccactcg tcaccatcta aaacagcttt tgtctgtgca
    12661 ccctgtgcat cagtaccagc ccccggctca gttaatgcga atgcacctaa cttctcgcct
    12721 gttgcaagtg gttttacata tttctgtttc tgctcttctg taccatatgt catgatcgga
    12781 tcgatgcaga gagatgtatg tgcagataca acaacacctg tggttgcgca aactttagat
    12841 aactcttcta cgcacataac gtatgtaaga ggatcacaac cctgtccacc atattctttc
    12901 ggaaccggga ttcccatgaa tccgtatttc tgcattttct taaccgtttc tgccgggaaa
    12961 gcttctgtct catctgtttc ctgtgcaagc ggttttactt ctgtttcagc aaattccttg
    13021 aataaggaac gcgccatttc atgtttcttg tctaaagtga aatccatgat ctaattttcc
    13081 tccataaata tttattttac tgagcatcga ccggagtctt tgtacgatca gcattgtata
    13141 catagaatcc cttgccgctc tttgctccaa ggttaccacc acgaaccatc ttacggatca
    13201 gtgggcatgc acgatatttg ctgtcgcctg tctctttgta aagtacatcc atgatagcaa
    13261 ggcagatatc aagaccgatg aaatcaccta actctaacgg tcccattggg tggttagcac
    13321 caagtttcat agctgcatcg ataccagcga tatcggaaac accttccatt ttgatgaatg
    13381 cagcttcgtt gatcattggg attaagatac ggtttacaac gaaaccagca gcctcattaa
    13441 cctgtaccgg agtttttccg atctcttcag aaattttctt aatagcggaa acagtttcat
    13501 ccggagtatt cacaccagcg ataacctcga tcagtttcat acggtctgcc gggttgaaga
    13561 aatgcatacc aaccattgga cggtttaatc cgtttccaat ctctgtaata gaaagactgg
    13621 atgtgttgga agcgaagata caatctggtt ttgcgatctc gtctaattct ttgaaagtag
    13681 ttttctttac ttccatattc tcgaaagctg cctctacgat cagatcacag tctttgcaaa
    13741 ggtcttcttt taaacctgga gtgatttttg caaggatgcc atcaacagct tcctgtgtca
    13801 tttttccttt ttctactaat cttgcataac cttttttgat cttctctttt ccgccttcag
    13861 cccactcctg cttgatatcg caaagtgcta cttcatatcc ttctacctga gcaaatgctt
    13921 ttgcaatgcc ctgtcccata gttcctgcac caataacgcc aaccttcatt tcggttacct
    13981 cctgaaaata attatgttaa ctgtatgttt gaaaaatgca atccccggga agtcattccc
    14041 cgggaattac tataaaaaac tattatctct caacgattgt agagcatccc ataccaccgc
    14101 cgatgcaaag tgtagcaaga cctctcttag cgtctctctt ctgcatctca tgcagtaatg
    14161 taacaaggat acggcatccg gaagcaccta ccgggtgtcc taatgcgatt gcaccaccgt
    14221 ttacgtttac ttttgacata tcgaatttca gatctcttgc tactgcgata gactgagctg
    14281 cgaatgcttc gtttgcttcg attaagtcca tatcatcaat agataaacca gtttttgcta
    14341 atacttttct tgtagaagca acaggtccaa cacccatgat ttcaggctca actccaccaa
    14401 gagcacctgc tacccatgta gccattggtg taacaccaag ctcttttgct ttttcttcac
    14461 tcataacaac gattgctgct gcaccatcgt tgataccgga agcgttacct gctgtaacaa
    14521 gtccgccctc tttaccggag cagcatttta attttgctaa agactcagca gttgtaccgg
    14581 cacgtggtcc ctcatctttc acgaaatcaa cgatctcttt tttcacttta acaggaaccg
    14641 gaacgatctc atcatcaaat ttgccttcag cgattgcttt ttcacatttc tgctggctgt
    14701 ttgctgagaa ctcgtcaagt tcttctcttg taatgccata tttatcacat acgttctctg
    14761 cagtgatcat catatgatag tggttgaatg catctgttaa tgcatcattt accattgtat
    14821 cgatgatcgt tgcattgttc atacgatatc caaaacgagc ttttgtcata gcatatggtg
    14881 ccatagacat gttttccata ccacctgcaa caacgatatc agcttcacct gccatgatca
    14941 tggtagctgc atcattgaca cattttaaac cagaaccaca aacaacgttg attgtgattg
    15001 ccggaacttc gattggcagt cctgctttga ttgctgcctg acgtgcaacg ttctgtccct
    15061 gagctgcctg gattacacaa cccattttta cctcgtcaac catctccggt ttaacgtttg
    15121 ctcttcttaa agcctctttg ataacgattg cacctaagtc tgctgctgga gtattgctta
    15181 atgctccacc cattttaccg attgcggtac ggcaggcacc tgctaaaact actttctttg
    15241 ccattttgaa ttctcctttc aatttaacac aaagctctgc ttttgcagag aatttgtttc
    15301 tttctcttta aagttgccga aaaatgctgt taatttttta acaatctttt ttaaaaaaat
    15361 aaattcaaac tttcggttga aatttatctt tttttctttc attttaaaaa
//
