LOCUS       c00071_gnlPro..         3262 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NEAMPNKN_71.
ACCESSION   c00071_gnlPro..
VERSION     c00071_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:17:07
            Original ID  :: gnl|Prokka|NEAMPNKN_71
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3262
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3262
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[290:3260]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      291..3260
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3262
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3262
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             291..2519
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_1"
                     /sec_met_domain="Gly_radical (E-value: 8.6e-48, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1172.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2535..3260
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg71_2"
                     /sec_met_domain="Radical_SAM (E-value: 7.6e-31, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
ORIGIN
        1 gtacaacggt tttcaagacc gccgcaatcg accactctgc cacctctcca aaacttcttt
       61 atcagaagtg cttttcgtta aaagcggtgc aaaggtacga atatatttta aacttacaag
      121 agttttcaca aaaaaaaatc acgaacatca tttccaaaga atagcaatat ctaggtattt
      181 tatgttgaaa agcataaaat agagcatatt tatgctgatt atatgaaaaa acgttgtaac
      241 tttgtgcgct ctaaagaaaa ggataactac aacctctaat tttatcattt atggaattaa
      301 ataagatctt taaagacggt ctttggagca cagaaatcaa cgtaagagat ttcgttagtc
      361 ataacatcac tccgtattac ggagatgctt ctttcctcga aggacctacc gaacgcacaa
      421 aagctgtatg gaaccgctgc ctcgaagcac tggcagaaga aagagccaat aacggagtac
      481 gctcactgga taatgtcacc gtctcaacca tcacttctca taaagccgga tatatcgaca
      541 aagaaaacga actgatcgtc ggtctgcaga cagacgaact tctgaaacgt gccatcaagc
      601 ctttcggagg tatcaatgtg gtgagcaaag cctgccacga aaatggcgtg gaagtggacg
      661 accgtgtaaa agatatattc actcactacc gcaagacgca caatgacgga gtatttgacg
      721 tatataccga agaaatccgt tcgttccgtt ctctgggatt cctcaccgga cttcctgaca
      781 actatgcccg cggacgtatt atcggcgact accgccgtat ggctctctat ggtatcgacc
      841 gactgattga ggcaaaaaaa gaagatttgc gtaaccttac cggtccgatg accgaagccc
      901 gcatccgcct gcgcgaagaa gtggcagagc aaatcaaagc attaaaagac atgaaggtaa
      961 tgggcgaata ctacggactc gacttgagcc gtcctgccta caccgcacaa gaagcagtac
     1021 aatgggtata catggcttac cttgctgccg taaaagaaca agacggtgct gctatgtcac
     1081 taggtaacgt ctcttcgttc ctcgacattt atatggaata tgaattgagc aaaggaacca
     1141 tcacagaatc tttcgcacag gagctgatcg accagtttgt catcaaactg cgtatggtgc
     1201 gtcacctgcg tatgcaatct tacaacgaca tctttgccgg cgacccgaca tgggtaaccg
     1261 aatccctggg tggacgcctc aacgacggac gtaccaaggt gacaaagact tctttccgct
     1321 tcctgcaaac actctacaac ctcggccctt caccggaacc gaacctgact gtattgtgga
     1381 gcccggaact gccggaagga ttcaaagaat tctgcgcaaa agtatctatc gacacttctt
     1441 ccatccaata tgaaaacgac gacctgatgc gtgaagtgcg ccagtctgac gattacggaa
     1501 tcgcatgctg cgtatcttat caggaaatcg gaaaacaaat ccaattcttc ggcgcacgtt
     1561 gcaacctggc aaaagccctg ttgcttgcca tcaacggcgg acgttgcgaa aacaccggta
     1621 cggtaatggt gaaaaatatc cccgtactaa ccagcgacac actgaacttc gaagaagtaa
     1681 tgagcaacta caagaaggta ttgacagaaa tcgcccgtgt ttacaacgaa gcgatgaaca
     1741 tcatccatta tatgcatgat aagtattact acgaaaaagc ccaaatggct cttgtagata
     1801 ccaacccacg catcaatctt gcttatggtg tagccggact ttctatcgcc ctcgattcac
     1861 tgtcggccat caaatatgcc aaagtgaccg cacgccgcaa cgacataggt ctgacagaag
     1921 gtttcgatat tcaaggcgaa ttcccttgct tcggtaatga caacgacaag gtagaccacc
     1981 tgggtgttga cctggtctat ttcttcagcg aagaattgaa gaaacttccg gtttacaaaa
     2041 atgcccgtcc tactttatct ttgctcacca tcacttccaa cgtgatgtat ggcaagaaga
     2101 caggtgctac tcccgacgga cgcgccaaag gtgtcgcttt tgctccggga gccaacccta
     2161 tgcacggacg tgacaagaac ggtgccatcg cctctttgag ttccgtagcg aaattacgtt
     2221 accgcgactc acaggacggt atcagcaaca ctttctccat cgtccccaaa tcactgggag
     2281 ctactgaaga ggatcgcgta gaaaatctgg tgacaatgat ggatggctat ttcaccaaag
     2341 gcgctcacca cctgaacgtg aacgtactga accgtgaaat gctttacgat gccatggaac
     2401 atccggaaaa atatccgcaa ctcaccatcc gtgtttccgg ttatgccgta aacttcgtga
     2461 agttgagccg cgaacatcaa ttggaagtaa tcagccgtag cttccacgaa cgtatgtaat
     2521 cagatctact caatatgacg ataaacgtac attcatacga aagtatggga acattcgacg
     2581 ggccgggctt acggctcgtc gtttttcttc aaggatgcaa tttccgctgc ctatattgcg
     2641 ccaaccccga cacaatagcc ggaaaaggag gcacacctac cccaccggaa gaaatcgtcc
     2701 gcatggctat gagccaacgt ccttttttcg ggaaacgcgg aggagtcact ttctccggag
     2761 gagaacctac gtttcaggcc aaagcactag tccctttagt gcgtgaactg aaagaaaagg
     2821 gaatacatgt atgcatagac agcaacggcg gtatctggaa cgaagaagtt gaagaacttt
     2881 tcaaactgac ggatcttgta ttgctggata ttaaagagtt caatccggca cgccatcaag
     2941 cactgaccgg aagaagtaac gagcaaacca tccgcacggc agcatggctg gaagaaaacg
     3001 agaaaccttt ctggctgcgt tatgtattag tgcccggata cagcgatttc gaggaagaca
     3061 tccgccgttt aggagaagct ctgggaaaat ataagatggt gcaacgagtg gaaatcctgc
     3121 cttatcatac attaggcgta cataagtatg aagcgatgga gcaggaatac aagctgaaag
     3181 acgtaaaaga aaataccccc gaacaacttg aaaaagccgc ggaggtattt aaagagtatt
     3241 tcactactgt ggtggtcaat ta
//
