LOCUS       c00381_gnlPro..         2242 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CMHPDAIK_381.
ACCESSION   c00381_gnlPro..
VERSION     c00381_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 10:44:47
            Original ID  :: gnl|Prokka|CMHPDAIK_381
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2242
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2242
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2240]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2240
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2242
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2242
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..620)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.2;
                     E-value: 3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg381_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.5e-34, bitscore:
                     106.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYK"
     CDS             complement(621..2240)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg381_2"
                     /sec_met_domain="Gly_radical (E-value: 4.7e-47, bitscore:
                     146.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.7e-198, bitscore:
                     646.4, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IIQSREEISKQIKALKAFEKMCASYGFDVTRPAQNAREAVQFVYL
                     AYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGKLTEEEAQELVDQLIIKLRIVRFLR
                     TPEYNDLFSGDPVWVTESLGGQGVDGRSLVTRTSYRYLHTLYNLGPAPEPNLTVLWFKN
                     APENWKRFCAKVSIDTSAIQYENDDLMRPDYGDDYGIACCVSPMKIGKQMQFFGARANL
                     AKCLLYAINGGRDERSGVQVAPMFEPVRGEYLEYDEVMAKYEQMMRWLAKVYVNALKII
                     HYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDTKVRVIRDERGLAVD
                     FEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQHETYRHAKPTQSILTITSNVVYGKK
                     TGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALASVAKLPFQHAHDGISYTFAVSPATL
                     GKEREVQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPEKYPQLTIRVSGYAV
                     NFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 tatttatagg ttcccatcgt atgataaggc aataattcca ctttgcgaac cactttatat
       61 gaactgacat accgagctaa cgcctccagc cactcgtcat tatccgtata tccggggaca
      121 ataacatggc gaatccaagt atctatgcca cgtctctcca attcatccaa gaacagaagc
      181 gtattatcct gcttgacacc cgccagtaac ggatgtaaat ccgggtttaa ggttttgatg
      241 tccaataaca ctaagtccgc ataatccaag acttcccaag cttttgaggt acatacgaaa
      301 ccagatgtgt ccaaagctgt gtgaagacct tgctcttggc acaaacggaa aaactctttc
      361 agaaactcag gttgcaataa aggttcccca ccggtaacag tcaccccccc acgggcgata
      421 aaactcttat accgtaaaac ttccgtcaat agctcgccgg gagtcatctg atatttcacc
      481 ttccctttcg ggttccaagt atccgggtta tggcaataca ggcaacgcaa cgggcagccc
      541 tgcataaaaa cgacaaagcg aattccggga ccatctacgg tcccgaaact ctctaatgaa
      601 tgtatcttac cttttatcac ttacagtgaa tggttaatgg ttcttgaaat aacatccaac
      661 tgttgttcac gagtcagttt cacgaagttt accgcatatc cggatacacg gatcgtaagt
      721 tgcggatatt tctcaggatg ttccatagcg tcgatcaaca aatccttatc aaatacgtta
      781 acattcagat gctgacctcc atccggagta aaatagccat ccaataagct aaccagatta
      841 ttgatctgca cttcacgctc ctttcccaac gtagccggag aaacagcgaa agtataagag
      901 ataccgtcgt gcgcatgctg gaatggaagc ttcgctaccg aagctagcgc agcgatagca
      961 cctttcgtat cacgaccgtt catagggttc gcgcccggag cgaaaggagt tccaccgata
     1021 cggccatccg gtgtcgtacc agtcttctta ccatatacca cattagaggt gatcgtaagg
     1081 atggactgcg taggtttcgc atggcgatag gtctcgtgct gacgcaaata ttccatgaac
     1141 ttctcggtga tctctacagc gatactatcc gtacgatcgt cgttattacc gaaaggaaca
     1201 tattcgccct cacgttcgaa atcaaccgcc aagccacgct cgtcacggat cacacgtact
     1261 ttcgtatcac ggatagcagc caacgaatcg gcaacgatag aaagaccggc gatacctgtc
     1321 gcacggatac gctctacatc tccatcgtga agcgacatct cgaatgcctc atatgcgtat
     1381 ttatcatgca tataatggat tatcttcaag gcgttcacat acactttcgc caaccagcgc
     1441 atcatttgct catacttagc catcacctca tcgtattcca gatactcgcc gcgaaccggc
     1501 tcgaacatcg gggctacctg tacgccgctg cgctcatccc gtcctccgtt gatcgcatat
     1561 aataaacatt tcgccaaatt cgcacgggct ccgaagaact gcatctgctt accgatcttc
     1621 ataggagaaa cgcaacaagc gataccataa tcatctccat aatccggacg catcaaatcg
     1681 tcgttctcat attggatagc cgatgtatcg atagagacct tggcacagaa gcgtttccag
     1741 ttctcgggag cgttcttaaa ccacagaact gtcaaattgg gttctggtgc cggacctaga
     1801 ttatataatg tatggagata acgataggaa gttcttgtta ccaaagaacg gccatccact
     1861 ccttggccgc ccaaggactc tgttacccaa accggatcac cggagaacaa atcattgtat
     1921 tccggcgtac gtaagaaacg aacgatacgc aacttgatga tcagctggtc taccaattct
     1981 tgcgcctcct cttcggtcaa cttaccctca cggatgtctt tctcgatata aatatccaaa
     2041 aacgtagaag tacgtccgat agacatcgcc gcgccatctt ggtcttttac cgccgccaga
     2101 taagctaagt acacaaactg aaccgcctca cgagcgtttt gtgccggacg agtcacatca
     2161 aagccatagc tagcgcacat tttctcgaac gctttcaaag ccttgatctg tttggaaatc
     2221 tcctcacggc tctggatgat ct
//
