GCA_021991685.1 - 5 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

gnlProkkaPCFPDMBM_4 (original name was: gnl|Prokka|PCFPDMBM_4)

Region	Type	From	To	Most similar known cluster		Similarity
Region 4.1	arylpolyene	110	40,292

gnlProkkaPCFPDMBM_12 (original name was: gnl|Prokka|PCFPDMBM_12)

Region	Type	From	To	Most similar known cluster		Similarity
Region 12.1	terpene	11,162	34,731	carotenoid	Terpene	100%
Region 12.2	NRP-metallophore,NRPS	248,590	304,518	enterobactin	NRP	100%

gnlProkkaPCFPDMBM_34 (original name was: gnl|Prokka|PCFPDMBM_34)

Region	Type	From	To	Most similar known cluster		Similarity
Region 29.1	HR-T2PKS	1	22,441

gnlProkkaPCFPDMBM_37 (original name was: gnl|Prokka|PCFPDMBM_37)

Region	Type	From	To	Most similar known cluster		Similarity
Region 32.1	thiopeptide	128,854	155,154	O-antigen	Saccharide	14%

Region	Type	From	To	Most similar known cluster	Similarity
Region 4.1	arylpolyene	110	40,292
Region 12.1	terpene	11,162	34,731	carotenoid	Terpene	100%
Region 12.2	NRP-metallophore,NRPS	248,590	304,518	enterobactin	NRP	100%
Region 29.1	HR-T2PKS	1	22,441
Region 32.1	thiopeptide	128,854	155,154	O-antigen	Saccharide	14%

Compact view

gnlProkkaPCFPDMBM_4 - Region 1 - arylpolyene

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Location: 110 - 40,292 nt. (total: 40,183 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

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core biosynthetic genes

additional biosynthetic genes

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Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

gnlProkkaPCFPDMBM_12 - Region 1 - terpene

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Location: 11,162 - 34,731 nt. (total: 23,570 nt) Show pHMM detection rules used

terpene: (Terpene_synth or Terpene_synth_C or phytoene_synt or Lycopene_cycl or terpene_cyclase or NapT7 or fung_ggpps or fung_ggpps2 or trichodiene_synth or TRI5)

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core biosynthetic genes

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transport-related genes

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resistance

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KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

gnlProkkaPCFPDMBM_12 - Region 2 - NRP-metallophore,NRPS

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Location: 248,590 - 304,518 nt. (total: 55,929 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

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transport-related genes

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resistance

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Gene overview

NRPS/PKS domains

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

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Similar known gene clusters from MIBiG 3.1

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 2, location 248589 - 304518:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ctg12_258

Search NORINE for peptide: strict or relaxed

AMP-binding (36..433): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: AMPSQGVVNK
Nearest Stachelhaus code(s):: diOH-Bz TMPSQGVVNK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

ctg12_266

Search NORINE for peptide: strict or relaxed

AMP-binding (460..860): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHFSLVDK
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

gnlProkkaPCFPDMBM_34 - Region 1 - HR-T2PKS

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Location: 1 - 22,441 nt. (total: 22,441 nt) Show pHMM detection rules used

Region on contig edge.

HR-T2PKS: (hr-t2pks-ksa and (t2fas or APE_KS2 or ketoacyl-synt))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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Gene overview

NRPS/PKS domains

KnownClusterBlast

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters from MIBiG 3.1

No matches found.

NRPS/PKS substrates

NRPS/PKS substrate predictions

ctg29_9

PKS_AT (12..294): pk

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 62.5%; inactive: 62.5%

minowa: Methoxymalonyl-CoA

Prediction, score:

Methoxymalonyl-CoA: 19.7
Malonyl-CoA: 16.9
Methylmalonyl-CoA: 12.9
Acetyl-CoA: 11.3
inactive: 10.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

gnlProkkaPCFPDMBM_37 - Region 1 - thiopeptide

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Location: 128,854 - 155,154 nt. (total: 26,301 nt) Show pHMM detection rules used

thiopeptide: ((YcaO or TIGR03604) and ((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or PF07366 or PF06968 or PF04055) or thiostrepton)

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core biosynthetic genes

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Gene overview

KnownClusterBlast

Thiopeptides

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Similar known gene clusters from MIBiG 3.1

Thiopeptide predictions

No core peptides found.

Thiopeptides

Core peptide predictions

No core peptides found.