LOCUS       gnlProkkaBDCAAGFA_135  14314 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BDCAAGFA_135.
ACCESSION   gnlProkkaBDCAAGFA_135
VERSION     gnlProkkaBDCAAGFA_135
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 10:17:36
            Original ID  :: gnl|Prokka|BDCAAGFA_135
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 28234
            Orig. end    :: 42548
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14314
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:9314]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..9314
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..14314
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14314
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             392..970
                     /locus_tag="ctg69_22"
                     /transl_table=11
                     /translation="MILRKKKSLNAGPINGTGEGVAHSKRWYVALVRMHHEKKVSEHLD
                     KMGIENFVPVQQEFHQWSDRRKLVTSVLLPMMVFVHVDPKERMEVLSFSTVSRYMVMRG
                     ESSPAVIPDEQMARFRFMLDYSEESISMNSSPLARGEKVRVIKGPLTGLVGELVNVDGK
                     SKIAVRLNMLGCACVDMPVGYVEPIMAAV"
     CDS             981..1349
                     /locus_tag="ctg69_23"
                     /transl_table=11
                     /translation="MDAIILQENIEGLLSLVRMLLPGGGSAGCVYLDDLSALQRSIHEK
                     INDLYSQRGETPEQDATLCLAILQGYNVSMYANPEDEERKQAVLTRSLSLLDVLPPSLL
                     KQQLSAVCHGMQELCEIN"
     CDS             1367..3778
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Poly_export"
                     /gene_functions="transport (smcogs)
                     SMCOG1186:polysaccharide export protein (Score: 32.3;
                     E-value: 6.8e-10)"
                     /gene_kind="transport"
                     /locus_tag="ctg69_24"
                     /sec_met_domain="Poly_export (E-value: 3.3e-18, bitscore:
                     56.9, seeds: 183, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIQRLFSILLFCLMLGSGSLMAQSMSDSQVLEYVKDGIRQGKEQ
                     KQLASELARKGVTKEQAMRVKQLYEQQNNVNTSKSTGTDINESRLREETKKNTSDMLED
                     HPTTQDLARGDQVFGRNIFNTRNLTFEPSVNLATPANYRLGPGDEVIIDIWGASQNTIR
                     QQISPEGTINIQKIGPVNLSGMTVSAANDYLKNALNKIYNGLNNTTDPTSDIRLTLGNI
                     RTIQINVMGEVVQPGTYALSSFSTVFHALYRAGGVSDIGSLRNVQLVRNGKNIATIDVY
                     EFIMKGNTQDDIRLQEGDVVIVPAYDVLVKISGKVKRPMRFEMKKDENLATLIKYAGGF
                     EADAYTRSLRVVRQNGEEYEVNTVKDIDYNIYKMRNGDVVTAEAILNRFTNKLEIRGAV
                     YRPGIYQLSGKLNTIRELVHEAQGLTGDAFLNRAVLYRQREDLTSEVVQIDIKSIMDGT
                     SPNLALMKNDILYIPSIHDLEDRGNVTVHGEVAHPDSYPYADNMTLEDLIIQAGGLKEA
                     ASTVRIDISRRIKNPRSTADNDTIGQMYTFSLKDGFVIDGQPGFILEPYDQVYVRRSPS
                     YQAQQNVAIEGEILFGGNYAMTSREERLSDLVNKAGGPTNYAYLRGAKLTRVANASEKK
                     RMGDVIRLMSRQLGEAMIDSLGIRVEDTFTVGIDLEKALSNPKSNADLVLREGDVISIP
                     KNTNTVTINGAVMVPNTVSYMKGKNVDYYLNQAGGCSDNARKSKKFIVYMNGQVTKVKG
                     SGKKQIEPGCEIIVPSKAKKKGNIANILGYATSFSSLGMMIASIANLIKK"
     CDS             3789..4937
                     /locus_tag="ctg69_25"
                     /transl_table=11
                     /translation="MIEKQSYQEPFSEVDKRKDDEMEIDLMAIFYKIITVRGVLCKAAG
                     VGVIIAIVVALSIPKQYTVKVTLSPEMGNAKGSNGLAGLAASFLGDGALIGESTDALNA
                     SLSADIVSSTPFLLELLEMEVSVSKKDDKMTLGSYLDEESSPWWDYLIGFPGIVIGGVK
                     SLFGEDTVPASGGRQGTIELTKEVNEKINFLKKKISASIDKKTAITSITVTLQNPQITA
                     VIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQRKYADYVDAHDNVVL
                     QSVRAEQERLQNDMSLAYQIYSQVANQLQVSRAKVQEEKPVFAVVEPAVVPLKPSGMGL
                     KIYILLFVFLSVSGTLVWVFFGKSLLASLRKELKNKEIEQDK"
     CDS             5001..5720
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 54.5; E-value:
                     1.6e-16)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg69_26"
                     /sec_met_domain="NTP_transf_3 (E-value: 4.9e-16, bitscore:
                     50.8, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CDIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             5741..7042
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 208.9;
                     E-value: 1.5e-63)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg69_27"
                     /sec_met_domain="CTP_transf_like (E-value: 6e-13, bitscore:
                     40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 6.2e-121, bitscore:
                     394.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 6.9e-63, bitscore:
                     203.6, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGISAIIIEDKVGLKKNSLFGTDVIQTQDT
                     IEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIHSKN
                     KSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAYPAM
                     MNVAKIILENERALEVRELCMPIKEILELIPGTK"
     CDS             7059..8183
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 58.1;
                     E-value: 1.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg69_28"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.3e-20, bitscore:
                     63.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.8e-20, bitscore:
                     64.0, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQHKKQGEVTLDLLKAMRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAII
                     IRKDVFGKYKLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKE
                     QGHEHDFLTVGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNY
                     YHILFNNGAHESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGP
                     VLLEIKVKIDSRENLGRPTTTPIENKEHFMDFLK"
     CDS             8193..9314
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg69_29_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg69_29"
                     /sec_met_domain="Aminotran_5 (E-value: 4.6e-31, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        8283..9290
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg69_29_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg69_29_Aminotran_5.1"
                     /locus_tag="ctg69_29"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             9311..9454
                     /locus_tag="ctg69_30"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYGNIN
                     LR"
     CDS             9657..11141
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg69_31"
                     /sec_met_domain="Polysacc_synt (E-value: 4.1e-31, bitscore:
                     99.7, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENLKQKTVSGIMWSAIERFSLQGVQFIIQIILARLLLPSDYGMI
                     GMLAIFLQVAQVFIDSGFTNALIQKKDRTEEDFATVFYFNILVAVLFYGILFFSASLIA
                     DFYNMPTLVLVIRFIALSLIINALSAIHRTKLIISVNFKTQSKISLGAAFISGVIGIWM
                     AYVGCGVWALVWQTLLNSIILTILFYCLVHWKPLHTFSKSSFERLFNFGSKLLISSLIN
                     TVYRNLYTIVIGKKFSVVELGYYTRAEQFAIFPSNNLNALVSRVAYPILSSIQDDDKCL
                     TNAYRNYIRLSSYIIFPLMVGLLVLANPIISLLLTDKWSGVVILLQILCLDWMFDHLSQ
                     INLNLLWVKGRSDLSLRLEIIKKTIAIFILFVSIPFGLEVMCWGRVIYSLIATYLNTYY
                     TNSLIDLTLKLQVKDVFPSLILSFIMGGIVFICISFFETDIVKIVIGCITGMSFYILLS
                     FLFKLDSFFCILTLIIKTKNIDENIKKDFSSNSKDN"
     CDS             11095..12354
                     /locus_tag="ctg69_32"
                     /transl_table=11
                     /translation="MKILKKIFQVIVKIINHCVPVDSRSLYFIPHPNCRTDKYDIINYS
                     SDNVLVLLNYLLKKDSAEYYKIYVEIYDSSRISEYQEYIYGINPNIRCTFLCACVGRFF
                     FNKVSFKDMLYAFFCFCRASKCFTATFYYDFSFKKRSQQIICLGYYTPFKDDYHMGDHS
                     YDKFRNTTCNSFDYSIATSCLSARIISIDCGISYDKFKVLGFPRNDLLISKNNCNVIKR
                     EISKFAGYDVTKYIVYTPTFRDYETVTEGNLRSILGYVDCDLLKLSKILLKFNAALILK
                     LHPLQNKTVLKKDLPKGILVFEQTYKYGLYDLLSFSDGMITDYTSTYFDFLLVNKPVIF
                     NFYDIEEYRRVRGFSFEPIEFFCAGDIVYNYNELIDAVMGLLAGKDIHAEHRRHISLLM
                     NQFQDDNSTKRICDIFLNEI"
     CDS             12363..13112
                     /locus_tag="ctg69_33"
                     /transl_table=11
                     /translation="MNYAFVVQARLGSTRLPGKILKPFYGNQSILDLMVHKLSAISNIP
                     VIIATTNSVINEPIEKKALALGVKCFRGEENDVLKRFIDVAEYFDIQGIFRICSDNPFL
                     DVHAARQLVEIAMKSCNDYISFDIDGTPSIKTHFGFWGEFVTLDALKRVIGFTDDLLYR
                     EHVTNYIYSHPELFNIQWISGSPVVSKHHNIRLTIDTLEDFSVAQRIYRDLQEKRVEIS
                     IEVIVDYVRRHAEYIQLMKQQIKKNSK"
     CDS             13112..14152
                     /locus_tag="ctg69_34"
                     /transl_table=11
                     /translation="MKNTYIIGEIGQNHNGSVDLAKLIIELISRPVHEDVFGMDFMPMN
                     AVKMTKRDLSEELAVSQMNQIYDNPNSFGRTYGEHRAFLELDDQAHFEIYKYAKSLGLD
                     FIETLCAKGCLSLLKLFTPDRLKVASRDLTNLPLLEALAETHIPIILSTGMAGQRELDN
                     ALDVITRYHSNIAILHCVSQYPTHPNNLNLRTITYLKKHYNKFEIGFSDHTIGISAATA
                     AVAMGAEIIEKHVTIDRHMKGTDQLGSLGPDGVNRMIRDIRIAECWLGTEDLYIEKGVE
                     KSKIKLERSIATRKYIPAGSIIQESDIHLLSPGDGYKWIDKKNVIGHFAKQDISANEII
                     YPDFIE"
ORIGIN
        1 gaaccggatg aatttgtctt acgtaaagcg gtgcggagta ggtcagtgta tctgatttag
       61 aagcttgata tacagtgttt atcggtgtgc gttacttctt aggtaacgaa caagcatagg
      121 gcaagcagaa aagggaactt ttttcaacga aaacatcaga agaggactgg tattcaggaa
      181 ataaagcaga agtgaaggtg tttttcatga attggaataa aaaaaagaca taacacggat
      241 attgtcattt tccgcctgtt atgcctgcat ttgattcatt ttccgaaatt aatttcattt
      301 ttgtttgcgt attttcataa atatgtccat ctttgcgcca tgaatcattc cgttacctaa
      361 gaagtaacgg ataaacatgg ggtaaaaaaa tatgatttta agaaaaaaga aatcactaaa
      421 tgctgggcct attaatggga caggggaagg cgtagcacac tcaaaacgct ggtatgttgc
      481 tttggttcgg atgcaccatg aaaagaaggt ttcggagcat ctagacaaga tgggtatcga
      541 gaatttcgtt cccgttcaac aggaattcca ccaatggagc gaccgtcgca agctggtcac
      601 ctccgtcttg cttcctatga tggtcttcgt acacgttgac cctaaggaac gcatggaagt
      661 cctgagtttt tctactgtca gccgttacat ggtgatgcgc ggcgagagta gtcctgctgt
      721 aattcctgac gagcagatgg ctcgtttccg tttcatgctt gattattcgg aagaatcgat
      781 aagcatgaac agttctcctt tggctcgtgg cgaaaaggtt cgcgtgatca aaggtccgtt
      841 gacgggtctt gttggcgaac tggtcaatgt ggatggtaaa agtaagattg ctgttcgctt
      901 gaatatgctg ggatgcgcct gtgttgatat gcctgtcggt tatgtagagc cgattatggc
      961 ggctgtttaa ggcaagggat atggatgcta ttattttaca agaaaatata gagggtttat
     1021 taagtttggt acgtatgctg cttcctggtg gggggagtgc aggctgcgtg tatttggatg
     1081 atctttcagc attacaacga tccatccacg aaaagattaa cgatttatat tctcaacggg
     1141 gggagacacc ggagcaggat gctaccctgt gtttggccat ccttcaggga tataatgtgt
     1201 cgatgtacgc caatccggaa gacgaagaaa gaaaacaggc tgttttgacc cgttctctgt
     1261 ctcttctcga tgttcttccc ccttctcttc ttaagcaaca gttatctgcc gtctgtcatg
     1321 ggatgcagga actgtgtgaa atcaactgaa acttttttat ttgtcaatga aaatacaacg
     1381 actttttagc attctactat tttgtttgat gctgggaagt ggctcgctta tggctcagtc
     1441 catgagtgac tcacaggtac tggaatatgt aaaagacggc atccgccaag gcaaggaaca
     1501 gaagcagctt gcttccgaac tagcccgtaa gggtgtaacc aaagaacagg caatgcgtgt
     1561 gaagcagcta tacgagcagc agaacaatgt aaatacctcg aaatcaacgg gcacggacat
     1621 caacgaatcc cgcctgcgtg aagagactaa gaagaatact tccgatatgc tggaggatca
     1681 tccgactact caggaccttg cccgtggtga tcaggtcttc ggacgtaaca tcttcaatac
     1741 ccgcaacctg acgttcgagc ctagtgtcaa ccttgccact cctgccaatt accgtcttgg
     1801 tcccggtgat gaagtcatca ttgatatctg gggtgcgagc cagaacacga tccgccagca
     1861 aatctctcct gaaggtacta tcaacattca gaagatcggc cctgtcaacc ttagcggtat
     1921 gacagtctct gccgccaatg actatctgaa gaacgccctg aataaaatat ataacggtct
     1981 gaataatact accgatccta cttccgacat ccgtctgacg ttaggtaata tccgtactat
     2041 tcagattaat gtgatgggag aagtcgtaca gcccggtacc tacgcccttt cttccttctc
     2101 taccgtcttc catgcgcttt atcgtgcagg cggtgtgagt gatatcggta gcctccgaaa
     2161 cgttcagttg gtacgcaacg gaaagaatat tgccaccatc gacgtctacg aattcattat
     2221 gaaaggcaac acacaggatg acatccgcct acaggaaggt gacgttgtga ttgttcctgc
     2281 ctatgatgta ttggtaaaga ttagcggcaa ggtaaaacgt cctatgcgtt ttgaaatgaa
     2341 gaaagacgag aaccttgcta ctctcattaa atatgccggc ggctttgaag ctgatgccta
     2401 cacccgttca ttacgtgttg tccgtcagaa tggtgaggaa tacgaagtca acacagtcaa
     2461 agatatagat tacaacattt ataaaatgcg taacggtgac gtagtaaccg ccgaagccat
     2521 ccttaaccgc ttcacaaaca aactggaaat ccgtggcgct gtctatcgtc ccggtatcta
     2581 ccagctcagc ggtaaactga acacgatccg cgaacttgtc catgaagctc agggtctgac
     2641 cggtgacgct ttcctgaatc gtgccgtcct ctatcgccag cgtgaagacc tgaccagtga
     2701 agttgttcag atagacatta agtctatcat ggacggcact tccccaaacc ttgccctaat
     2761 gaagaatgac atcctctaca ttccaagtat ccatgacctg gaagaccggg gtaatgtcac
     2821 agttcacggt gaggttgccc accctgattc ctatccttat gcggataaca tgacacttga
     2881 ggatctgatc attcaggccg gtggcttgaa agaagccgcc tctactgtcc gtattgatat
     2941 atcgagacga atcaagaatc ctcgtagtac tgcggataat gatacgattg gtcagatgta
     3001 taccttctct ttaaaggatg gttttgtaat agatggtcag cccggtttta tccttgagcc
     3061 ttatgatcag gtttatgtcc gtcgcagtcc gagctatcag gcacaacaga atgtagccat
     3121 tgaaggtgaa atccttttcg gaggaaatta cgccatgacc agccgtgagg aacgtctttc
     3181 cgatttagtg aataaagccg gcggtccgac caattatgcc tatctgcgtg gtgcgaaact
     3241 gacccgtgtg gctaacgcta gtgaaaagaa acgtatgggt gatgtaattc gccttatgag
     3301 caggcaattg ggcgaagcca tgattgactc tttgggtatt cgtgttgaag atacttttac
     3361 ggttggaata gatttggaaa aagccttgag caatcctaaa agtaatgccg atcttgtctt
     3421 acgtgaaggt gatgtgatct ccatacctaa gaatactaat actgtaacta tcaatggtgc
     3481 tgtcatggtt ccgaatacgg tttcttatat gaagggaaag aatgtggatt attacctgaa
     3541 tcaggccggt ggctgttctg ataatgcaag gaagagtaag aagttcattg tttacatgaa
     3601 tggtcaggtg acgaaggtta agggtagcgg taagaaacag attgagccgg gatgtgagat
     3661 tattgtgcct agtaaggcta agaagaaagg gaacatagct aatattttag gttatgctac
     3721 ttccttcagt tctttgggaa tgatgattgc ttctattgct aatttgataa agaaataaat
     3781 gacctattat gattgaaaag caatcttatc aagaaccgtt ttctgaagtg gataaaagga
     3841 aagatgatga aatggaaata gacttgatgg ctatttttta taagattatt actgttcgtg
     3901 gagtactatg caaagctgct ggagtgggag taataattgc tatagtggtg gcattgagca
     3961 ttcccaagca atatacagta aaggttaccc tttctcctga aatgggtaat gcgaagggaa
     4021 gtaatggttt agccggactt gctgcctcgt ttttgggaga tggggctcta ataggggaaa
     4081 gcacagatgc cttgaacgct tctctttcag cagatattgt ttcttcaact ccttttttat
     4141 tggaactttt agaaatggaa gtctccgttt ctaaaaagga tgataaaatg actttggggt
     4201 cttatctcga tgaagagtca tctccttggt gggattatct tataggtttt ccgggcatag
     4261 taataggagg agttaaatca ttattcggag aagatacggt tcctgccagt ggagggcggc
     4321 aaggaactat cgaattgacg aaagaagtaa atgagaagat taattttctt aaaaagaaga
     4381 ttagtgcatc cattgataag aaaaccgcca taacgagcat tacggtcacc ttacaaaatc
     4441 cgcagatcac tgcggttata gcagattctg tcgttcataa attacaggaa tatattattg
     4501 ggtatcgaac ttcaaaagta aaagaagact gtgcgtattt ggaacggttg tttaaagaac
     4561 gtcagcagga atattatgct gctcaaagaa aatatgcgga ttatgtagac gcccatgata
     4621 atgtggttct tcaaagtgta cgtgctgagc aggagcgttt acagaacgac atgagtttgg
     4681 cttatcagat atatagtcag gttgctaatc agttgcaggt atccagagcc aaagtacagg
     4741 aggaaaagcc agtctttgca gtagttgagc ctgccgttgt acctttgaaa ccttcaggta
     4801 tgggactcaa aatttatatt ttattatttg tgttcctgtc cgtatctggt actctcgtgt
     4861 gggtgttttt tggcaaaagt ctgctggcta gcttgagaaa agaactgaaa aataaggaga
     4921 tagaacagga taaatgatgc taaaacgtcg tttacaacat tcttacatca attaaattca
     4981 cttactatat tattaaacga atgatacgta cagctatgat aatggctgcg ggcatgggaa
     5041 cccgttttgg ccaatataca gaattgatac ctaaagggtt cgtcaaagtg ggaggtattc
     5101 caatgatagt tcgttctatt gatactttgt tatcatgtga cattgagcga attgtaatcg
     5161 gtacaggata taaacaagaa gtttatgaag aattgaagac agactatcct atgcttgaaa
     5221 cctgttttag tcctcgttat gctgaaacaa atagtatgta tactttatat aatacgcggg
     5281 agattctggg caatgatgat tttttattgt tagagtcaga tttgattttt gagaaacaag
     5341 caataatgag cttattggag tgtcctgcgg cagatgcaat gctaataact ccagtgacaa
     5401 agtttcaaga tcagtattat gtggaacatg atgataattt tcgcctttct tcatgttctg
     5461 tcaataagaa taaactaaac gctaaaggcg aattggtcgg catccataaa ctatccggtt
     5521 ccttttacaa aataatgtgt gctgattatg cgagtattgt tgatgaacaa cccaatttgg
     5581 gatatgagta tgaattacta cgaatatctt gttctcaatc tcctgtttat gtgcataaag
     5641 tagaagggct gaaatggtat gaaatcgatg acatatcaga tttggaatat gcagagaaat
     5701 atattgttcc gtattgttaa ttaaaataat atatcaaatt atgagtaaga cagtttatat
     5761 tgggatgact gcggatatta tgcatccagg acttatccgc attattaatg aggctactaa
     5821 atacggtgat gttataattg gtcttcttac agataaagct attgcggagc ataaacgcct
     5881 tccttatctg acctatgaac aacgaaaaga ggtagttcag aatattaaag gcgtatgtaa
     5941 agttgttcct caagaagaat ggagctatgt tgagaatctc aaacgcatta aacctgacta
     6001 tataattcat ggtgacgact ggaaaaccgg gcctttacgt gaagaacgtg tgcgcgtatt
     6061 tgaagtgatg aatgaacagg gaggaaaggt tatcgaaatt ccatatactt tgggaattaa
     6121 ttcatcttct ttggataaag atattaaggc tataggtaca acccctgatg tacgtttaaa
     6181 atcacttcgt cgtttgataa atgccaaacc cgtagttcgt attttggaag ctcatgatgg
     6241 actatgtggc ttgataattg aaaatcagga aatattaaaa ggggataaga gggaagtatt
     6301 tgacggaatg tggtctagta gccttacgga ttcaaccagt aaaggtaaac ctgatattga
     6361 ggcggttgat ttaactaccc gattgcaaga tttgaataat attctggagt gtactactaa
     6421 acctattatt tttgatggag atacaggtgg taaaattgaa catttcgtat ttacagtacg
     6481 cactttagaa cgtcatggaa tttcagctat aataattgaa gataaagttg gattaaaaaa
     6541 gaattcattg tttggtacag atgttattca aactcaagat acaatcgaag gtttctgtaa
     6601 taagatcaaa gcaggaaagg catctcagat tactgatgat tttatgatta tcgctcgtat
     6661 tgagagcctt atagcgggaa agccggtaag tgatgcttta gagagagctt ttgcatatgt
     6721 ccaagcggga gcggacggta ttatgataca tagcaaaaat aagtcgggtg aagatataaa
     6781 agaattttgt ttggcgtttc gaaaacagta tgctcatgta cctatcgtgg ttgtgcctac
     6841 tacatatgat catatttatg agtctgaatt gtgtgattgg ggtgtgaata taattattta
     6901 tgctaatcat atgcttcgtg ccgcttatcc tgctatgatg aatgtcgcaa agataatatt
     6961 agagaatgaa cgtgctttag aggtgcgcga actgtgtatg cccataaaag aaatattgga
     7021 actgattcca ggtactaaat aaaaagagga atatcattat gttaagcgta gaaaaagttt
     7081 atgaaacgtt tctgagtcat gacgttgatt tttttacagg agttccagac tcattactaa
     7141 aaaatatttg tgcctatatc acagaccata cttcacgtga gaaacatatc atagctgcta
     7201 atgaaggtgc ggctgtagga attgcttcag gttattatat ggcatcaggt aaggttcctg
     7261 tggtttatat gcaaaattcc ggcttgggta atactgttaa tcctctgctg tctcttgcag
     7321 atgaacaagt atacagtttt ccaatgcttt tgatgattgg ctggcgtggt gaaccgggaa
     7381 caaaagatga acctcagcat aaaaaacagg gtgaggtaac tttagatttg ttgaaagcaa
     7441 tgcggattcc ttatataatt cttgattcaa atgagaatga tgcatttatt cagctccatg
     7501 atatcataca atcagcaaaa actaagaata ttcctcatgc aataattatt cggaaagatg
     7561 tttttggcaa atataaactt caaaaagagt tcggatgtaa ttatccttta tctcgtgagg
     7621 atgctttgca aatagttgtg gattatctgc ctgaaaattc agtggtcgtt tctactactg
     7681 gcaagctttc tcgtgagtta tttgaatatc gagaaatgaa agagcaaggt catgaacatg
     7741 attttctcac ggtaggctct atgggacata gttcatcaat ctctttgggt attgctatag
     7801 caaagcaaga tagacctgtt tattgcttgg atggtgatgg agcatttatt atgcatttag
     7861 gggcaattag taacattggc gatttatctc ctaaaaacta ttatcatatt ttgtttaaca
     7921 atggtgctca cgagtctgta ggaggacagc ctactttagg atttagttta gatattccgg
     7981 ctattgtccg ggggagcgga tataagcata cttatactgt ttgtacaaaa gttgaaattg
     8041 aagaagcaat gaagcagctt ccaaagttat gtggtcctgt tttgttggaa ataaaggtta
     8101 aaatagattc tcgggagaat ttgggacgtc ccacgactac tcctatagag aataaggagc
     8161 attttatgga ttttttaaaa taaatatatt cgatgaaaat agaaagaaaa atattattaa
     8221 atcctgggcc tgccactact actgacagtg taaaaatggc acaagttgtt ccggatattt
     8281 gtcctcgcga gaaagaattc gcaggtatga tgaaacaact tcgggacgat ttggtcagag
     8341 ttgctcatgg gaatttggaa aaacataccg ctgtattatt ttgtggctca ggaaccatca
     8401 atattgatat atgtctaaat tctcttcttc ctgaagacaa aagggtgctt atagtgaata
     8461 atggggcata tagtacccgt gctgtagaag tgtgtcaata ttatggtttg cctcatatta
     8521 atttggaatt ttccgtttac gaacgtcctg atttatcggt ggtggaaaat gtgttgcaag
     8581 aaaatcctga tatagctatg gtttatacta ctcatcacga gacgggtaca ggaattctga
     8641 accctattcg cgagatgggc gctatagctc ataaatatca ggctaaattt gtcgtcgata
     8701 ctacttcttc tttgggcatg attccttttg atatagagaa agataatgtg gatttttgta
     8761 tggcatctgc tcaaaaagga cttcaagcga tgacaggtct ttcttttatt ataggcaatg
     8821 aggaacagat taagttttca aaaaaatatc ccaagcgttc ttactattgt aatctttatt
     8881 tgcagtatga gaattttgag agaacaggtg aaatgcattt tactccaccg gtacaaacta
     8941 tttacgccac tcgtcaggca ttaaatgaat attttgaagt gggtgaagaa gctaagtttg
     9001 cccgtcacaa aagagttttt gaggctattc attcaggtat aaatgaaatt ggtcttaaaa
     9061 gtgtaataaa gcgtgaatgg cagtcaggac tggttgtctc agttcaatac ccagaagatc
     9121 ccaactggga ttttgagaaa atacatgatt attgttatga gcgtggattt acgatttatc
     9181 caggtaagat atcaactgag gatactttta gaatctgtgc tttaggagag atagaagtag
     9241 atgatattga gaacttcttt aaagtactaa aagctgcatt aaatcattat aacgtcacat
     9301 taccgatgtc atgattgcag aaatattatt gaagattcgt catttttatt tttcccttcg
     9361 tggtatacac ttccatccgg aaagtgttat ttctggagtg cctattcgta gctttcgctt
     9421 agtaggcaaa tatggcaata ttaatttgag gtgatgcatt aggagttctt tattcttata
     9481 aaaaggcatc ggtagttata ggagataatg tttgtgtagg gtaatttgct tttagtggga
     9541 gagaccatat tagaattgcg gtaaggggcg aagaagataa taaccaatta gtgagaatat
     9601 tggaaaaata taattgatat cggtaaatgt agttgttttt atactgtatt taagtaatgg
     9661 aaaatctaaa gcagaaaact gtatcaggaa taatgtggag cgctatagaa cgtttctctt
     9721 tacaaggagt acagtttata attcaaataa tattagctcg tttgttacta ccatcggatt
     9781 atggtatgat cgggatgtta gctatttttc ttcaagtcgc tcaagtattt atagatagcg
     9841 gttttacaaa tgctttaatc cagaaaaaag atagaactga agaggatttt gccactgtct
     9901 tttattttaa tattcttgtc gcagtattat tctatggtat attgtttttt tcggcttctt
     9961 tgattgcaga cttttataat atgccgactt tagttcttgt aattcgtttt atagcattgt
    10021 cgttgataat aaatgcttta tcggctattc acagaacgaa attaattatt tctgtaaact
    10081 ttaaaactca atctaaaatt tcattaggtg cagcttttat atcaggcgtg attggaatat
    10141 ggatggctta tgtcggttgc ggggtttggg ctttagtttg gcaaactctc ttaaatagta
    10201 taattttgac aattttgttt tactgtttag ttcattggaa acctttacat actttctcca
    10261 aaagttcttt tgaaagatta ttcaatttcg gatctaaatt attgatctca agtcttatca
    10321 atacagtata tcgtaatctg tataccattg taataggaaa aaagttttct gttgtagagc
    10381 ttggttacta tacaagagca gaacaatttg ctatttttcc ttccaataat ttaaatgctt
    10441 tagtttctag agtagcgtat cctattttaa gttccattca agatgatgat aaatgtttaa
    10501 caaacgcata tcgaaattat atcagattat cctcatatat aatttttcct ttaatggtag
    10561 gtttacttgt tttggctaat cctattatta gtctattgtt aacagataaa tggtctgggg
    10621 ttgttattct tttacagata ttatgtttgg attggatgtt tgatcatttg agccaaatta
    10681 atttgaattt attatgggtt aaaggaagat ctgatctgtc gttacgtctt gaaataataa
    10741 aaaagacgat tgctatattc attctttttg tgtcaattcc atttgggctt gaggtgatgt
    10801 gttggggtag agttatttac tctctgatag cgacatattt gaatacatat tatactaatt
    10861 ctcttattga tttgacctta aagttgcaag ttaaagatgt atttccaagt ttaatactct
    10921 ctttcataat gggggggatc gtttttatat gtatttcttt ctttgaaact gatatagtaa
    10981 aaattgtgat tggatgtatt actgggatgt ccttctatat tttactttct tttcttttta
    11041 agctagattc ttttttttgt attttaacat tgatcattaa aactaaaaac atagatgaaa
    11101 atattaaaaa agatttttca agtaatagta aagataatta accattgtgt gccagttgat
    11161 agtagatcat tgtattttat tcctcatcct aattgtagaa cagataaata tgatattata
    11221 aattattcgt cagataatgt tctcgttctg ttaaattatt tgctaaaaaa agatagtgct
    11281 gaatattata aaatatatgt tgaaatttat gattcttctc gtatttcaga atatcaagaa
    11341 tatatttatg gcataaatcc taatattagg tgtacttttt tatgtgcatg tgttggacgt
    11401 tttttcttta ataaagtatc ttttaaggat atgttgtatg cctttttttg tttttgtaga
    11461 gcaagtaaat gttttacagc tactttttat tatgattttt catttaaaaa acgctctcaa
    11521 cagattattt gtttggggta ttatactcca ttcaaagatg attatcatat gggagatcat
    11581 tcttatgata aatttcgtaa tacaacatgt aactcatttg attactctat tgctacttct
    11641 tgtttaagtg ctagaataat ttcaatagat tgtggaatct catatgataa attcaaagtc
    11701 ttaggatttc ccagaaatga tttgttgata tcgaaaaata attgcaatgt tattaaaaga
    11761 gaaatttcga aatttgcggg atatgatgta actaaatata tagtttatac tccaactttt
    11821 cgtgattatg agactgttac tgaaggtaat ttgcgttcaa tactcggata tgttgattgt
    11881 gatttgttga agttatcgaa aatactattg aagtttaatg cggctttaat tcttaaatta
    11941 catcctttgc agaataaaac agtcttaaaa aaagatttac ccaaaggtat acttgttttt
    12001 gaacaaacat ataaatatgg attgtatgat cttttgtcat tttcagatgg aatgattact
    12061 gattatactt ctacctattt tgatttttta ttggtaaata agccagtcat atttaatttc
    12121 tacgatattg aagaatatcg tagagtaaga ggtttctcat ttgagccaat agaatttttt
    12181 tgtgcaggtg atattgttta caattataat gaattgatag atgctgtgat gggacttctg
    12241 gctgggaaag atattcatgc tgaacatcga cgtcatattt ctttattgat gaatcaattt
    12301 caagatgata attcaacaaa gagaatatgt gacatatttt taaatgaaat ataataaata
    12361 aaatgaatta tgcatttgtg gtgcaggcac gtttaggttc tacacgtttg cctggaaaaa
    12421 tattgaaacc tttctatggt aatcaatcga ttcttgactt aatggttcat aagctttctg
    12481 ctatatcgaa tattccagta attattgcta caacaaattc tgttatcaat gaacctattg
    12541 aaaagaaggc acttgcatta ggggtaaagt gcttcagagg tgaggagaat gatgttttaa
    12601 aacgattcat tgatgttgct gaatattttg atatacaggg tatattccga atatgttctg
    12661 ataatccatt tttagatgta catgcggcaa ggcaattggt cgaaatagca atgaaatctt
    12721 gtaacgatta tataagtttt gatattgatg gtactccatc aattaaaact catttcggtt
    12781 tttggggaga atttgtaacg cttgatgcgc tgaagcgagt tataggattt actgatgatt
    12841 tattgtatcg tgaacatgtt actaattata tatatagtca tcctgaactt tttaatattc
    12901 aatggatttc aggatctcca gtcgtgtcaa aacatcataa tattcgactc acgattgata
    12961 cgttagaaga tttttctgta gcacagagaa tatataggga tttacaagaa aaaagagtgg
    13021 aaatatcgat agaagtgatt gtagactatg ttcgaagaca tgcagaatac attcaactga
    13081 tgaaacaaca aataaaaaaa aattctaaat aatgaagaat acttatatta ttggtgaaat
    13141 agggcagaat cataatggtt ctgttgactt ggctaagttg attattgaat taatctcacg
    13201 acctgtacat gaggatgttt ttggtatgga tttcatgcca atgaatgctg ttaaaatgac
    13261 taaaagagat ttatcagaag aattggctgt ttctcaaatg aatcaaatat atgacaatcc
    13321 taattctttt gggcgtactt atggtgaaca tcgtgctttt cttgagcttg atgatcaagc
    13381 gcattttgag atttataaat atgctaagtc attaggatta gattttatcg agacgttatg
    13441 tgccaaagga tgtctgtctt tgcttaaact gttcacaccg gataggctaa aagttgctag
    13501 tcgtgatttg actaacttac cattgcttga agcattagca gaaactcata ttcctattat
    13561 attatcgaca ggaatggctg gtcaaagaga acttgacaat gcattagatg tgataacgag
    13621 atatcatagt aatatagcta ttctacattg tgtatctcag tatccaactc atccaaataa
    13681 tttgaatcta cgtacaataa catatttgaa gaagcattac aataagtttg agataggttt
    13741 ttctgatcat acaataggta tttctgctgc tacagctgct gtagcgatgg gagcggaaat
    13801 tatagaaaag catgtaacaa ttgatcggca tatgaaagga acagaccagc ttggctcttt
    13861 aggtccagat ggggttaatc gtatgattcg agatattcgc atagcagagt gttggcttgg
    13921 tactgaagat ctttatatag aaaaaggggt ggagaagtct aagataaaac ttgaacgttc
    13981 tattgccact cgaaaatata ttccagcagg tagtattatt caagagtcgg acattcattt
    14041 actgagtcct ggtgatggtt ataaatggat agataaaaaa aatgtaattg gtcattttgc
    14101 taaacaagat atatcagcga atgaaataat atatcctgat tttattgaat agatatattc
    14161 ctactgcaac ttgtattacg gtatagagta ctgcctgcta taagctaagt agtgattaat
    14221 aaataagggt aaaatactaa aaaaatgaaa gagataaagc ttatacttac agatattgat
    14281 ggcgtttgga ctgatggcgg aatgttttat gatc
//
