LOCUS       c00275_gnlPro..         2724 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|APNMGPIO_275.
ACCESSION   c00275_gnlPro..
VERSION     c00275_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 06:19:04
            Original ID  :: gnl|Prokka|APNMGPIO_275
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2724
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2724
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[335:2724]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      336..2724
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2724
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2724
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             336..2564
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg275_1"
                     /sec_met_domain="Gly_radical (E-value: 2.7e-47, bitscore:
                     147.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-282, bitscore:
                     925.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQNEWRDFNGGAWENEVNVRDFIQRNYKPYDGDSSFLEGPTEDTT
                     ALWQDVLELSKQEREAGGVLDMDTKIISTITSHGPAYLDKDKEKIVGFQTDKPFKRSLQ
                     PYGGIRMAIKACEDNGYKVDPEVVEYFTTHRKTHNAGVFDAYTPEMRACRSAHIITGLP
                     DAYGRGRIIGDYRRPALYGVDRLIEDKQEQLDSTRTIMYSDVIREREELSEQIRALKML
                     KELAKIYGCDISKPATNVLEAAQAVYFAYLAAVKEQNGAAMSLGRTSTFLDIYAERDLR
                     EGTFTEKEIQEIIDQFIMKLRCVKFARTPEYNSIFAGDPTWVTESIGGIGVDGRHMVTK
                     MSYRYLNTLNNIGAAPEPNLTVLWSVKLPENFKKFCAEISIKHSAIQYENDDIMRVVHG
                     DDYGIACCVSSMRIGKEMQFFGARANLAKCLLYAINGGVDEISGKQVGPKYRPITSEYL
                     DYDEVWDKYKDMMKWLAGVYVNALNIIHYMHDKYCYERMQMALHDKQVRRWFATGIAGF
                     SVVADSLSAIKYAKVKPIRDENGITVDFEIEGDFPKYGNDDDRVDEIAKEVLHTFIKYV
                     KGNHTYRGGIATTSILTITSNVSYGKNTGATPDGRKKGVAFAPGANPMHGRDKNGAVAS
                     LASVAKMPFMDAQDGISNTFTIVPDALGKTEESSETNLVALLDGYAEKGGHHLNVNVLN
                     KETLLDAQKHPEEYPQLTIRVSGYAVNFIKLTKEQQDEVIARTFHELM"
     CDS             2581..2724
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg275_2"
                     /sec_met_domain="Fer4_12 (E-value: 2.6e-14, bitscore: 40.9,
                     seeds: 82, tool: rule-based-clusters)"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-08, bitscore:
                     22.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGYIHSLESFGTVDGPGVRFVVFFEGCPMRCQYCHNPDTWVLTDG
                     KQM"
ORIGIN
        1 aattgttata tgactgacgg aaaaagtgga agaaccacat gaagtataac gtttacaatg
       61 ccgaccgtct gggcgagata acccggactg tcggtttctt ttattcttaa agatattctt
      121 cttacaggaa tccttttgta tgaaaaaaag aacagttccg gaaacgaagc ggatattgac
      181 aagcagtgat ggaacatgat atattcactg cgttagaact agctaacaaa agttagaaat
      241 aactaccaat attttccagc aatacaaaaa tattggggga tcagatccgt tttatacagg
      301 taaaccatag ttttcagcaa acaggaggaa aaataatgca aaacgaatgg agagatttta
      361 atggtggagc atgggagaat gaagtaaacg tcagagactt catccagaga aactataagc
      421 catatgatgg tgacagctct ttcttagagg gaccgacaga ggataccaca gcactctggc
      481 aggacgtatt agagctttca aagcaggaga gagaggccgg cggcgtgctc gatatggata
      541 caaagatcat ttccacgatc acttcccatg gacctgcgta cttagacaaa gataaggaga
      601 agatcgtagg tttccagaca gataaaccat tcaaacgttc cttacagcct tatggcggta
      661 tccgtatggc aattaaggca tgtgaagata atgggtataa agtagacccg gaagtggtag
      721 aatatttcac aacacacaga aagacacaca atgcgggtgt gtttgacgct tatacaccgg
      781 agatgagagc ctgccgttcc gcacatatca tcaccggact tccggatgca tacgggcgtg
      841 gacgtatcat cggcgactac agaagacctg cactttacgg tgtagaccgt ctgatcgaag
      901 ataagcagga gcagttagat tccacacgca ccatcatgta ttccgatgtt atccgtgagc
      961 gtgaggagtt atccgagcag atcagagccc ttaaaatgtt aaaagaatta gcaaagatct
     1021 atggatgcga tatttccaaa ccggcaacaa atgttcttga ggcagcacag gctgtatatt
     1081 ttgcctacct tgcagcagtc aaagagcaga acggtgcggc aatgagtcta ggacgtacct
     1141 ctacgttctt agacatctat gcagagcgcg accttcgtga gggtacattc acggagaaag
     1201 agattcagga gatcatcgac cagttcatca tgaaactgcg ctgcgtaaaa tttgcccgta
     1261 caccggagta caacagtatt ttcgccggag atcctacctg ggtaacagag tctatcggtg
     1321 gaatcggagt agatggaaga catatggtaa ccaagatgtc ttaccgttat ttaaatacct
     1381 taaacaacat cggagcagca ccggagccaa acctgactgt tttatggtcc gtaaaacttc
     1441 cggagaactt caaaaagttc tgtgcagaga tttccatcaa acattctgcg atccagtacg
     1501 aaaatgatga catcatgcgt gtggttcacg gcgatgacta cggtatcgca tgctgcgtat
     1561 cttccatgag aatcggaaaa gagatgcagt tcttcggagc ccgtgcaaac cttgcaaaat
     1621 gtttactcta tgcgatcaac ggtggtgtgg atgagatcag cggcaaacag gtaggaccga
     1681 aatatcgtcc gatcacttcc gagtatcttg actacgacga ggtatgggat aaatacaaag
     1741 acatgatgaa atggcttgca ggtgtctatg tcaatgcatt aaacatcatt cactatatgc
     1801 atgacaaata ctgctacgag agaatgcaga tggcattaca tgacaaacag gtaagacgtt
     1861 ggtttgcaac cggtattgca ggattttccg tagtagcaga ttccctttct gccattaaat
     1921 atgcaaaagt aaaaccgatc cgtgatgaga acggtattac agtagacttt gagattgagg
     1981 gagatttccc gaaatatggt aatgatgatg accgtgtgga tgagatcgca aaagaggttc
     2041 tgcatacatt catcaaatat gtaaaaggaa accacactta ccgtggaggt atcgcaacaa
     2101 cttccattct taccatcaca tccaacgtat cctacggtaa gaatacagga gcaaccccag
     2161 atggaagaaa gaaaggtgtt gcgtttgctc cgggtgcaaa tccaatgcac ggacgtgata
     2221 aaaacggtgc ggttgcttcc ttagcttccg ttgcaaaaat gccatttatg gatgcgcagg
     2281 atggtatttc caacaccttt accatcgtgc cggatgcact tggaaagacg gaggaaagct
     2341 ctgaaacaaa ccttgtggca ctgcttgacg gctatgcaga aaaaggcggt caccacttaa
     2401 atgtaaacgt attaaataag gaaacactgc ttgatgcaca gaagcatccg gaggaatatc
     2461 cgcagcttac gatccgtgta tcaggttatg cagtaaactt catcaaactg acaaaagagc
     2521 agcaggatga ggtaattgca agaactttcc atgaattgat gtaataataa ggagattatt
     2581 atgggctata ttcattctct ggaatcattt ggtaccgtgg acggtccggg ggtgcgtttt
     2641 gtcgtattct tcgagggctg cccgatgcgc tgccagtatt gccacaaccc ggatacatgg
     2701 gtgctgactg atggaaaaca gatg
//
