LOCUS       c00031_gnlPro..         5805 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EKFCBCIP_31.
ACCESSION   c00031_gnlPro..
VERSION     c00031_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:40:06
            Original ID  :: gnl|Prokka|EKFCBCIP_31
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5805
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5805
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:5710]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..5710
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..5805
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5805
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             3..1433
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg31_1"
                     /sec_met_domain="ILVD_EDD (E-value: 1.7e-128, bitscore:
                     418.0, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IVVPGDISSATFFIVAGLITPNSCIRLKNVGINPTRDGILRVCKD
                     MGADVTLENVIDNGGEPTADIVVKTSRLKGTVIGGEVIPTLIDEIPVIALLAAFAEGET
                     VIKDAAELIVKNAKLYYEEGDESVLPRSIATRQAFLNAMTLDIAMGGSTNTVLHLLAIA
                     HEAEVDFKMDDIDMLSRKAPCLCKVAPNTQKYHIQDVNRAGGIIAIMDELAKGGLIDTS
                     VRRVDGMSLAEAINEYSITSPNVSEKAIKKYSSAAGNRFNLVLGSQGMYYKELDKDRAN
                     GCIRDLEHAYSKDGGLAVLKGNIAQDGCVVKTAGVDESIWKFTGPAKVFDSQEAACEGI
                     LGGRVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGKECALITDGRFSGGTSGLS
                     IGHISPEAAAGGNIGKIVDGDIIEIDIPARKINVRLTDEELAARPMTPVTRDRYVPKSL
                     KAYASMVSSADKGAVRII"
     CDS             complement(1358..1507)
                     /locus_tag="ctg31_2"
                     /transl_table=11
                     /translation="MTVENEWFTIIALSAAQLTITYQLYYIILTAPLSAELTMLAYAFR
                     LFGT"
     CDS             1548..3242
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg31_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.9e-48, bitscore:
                     152.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDLITGAEALMRSLEHQGVTTIFGYPGGSIMPVFDALYDHQNIL
                     NHILVRHEQGAAHAAQGYARVSGDVGVCLVTSGPGATNTVTGIADAMIDSTPIVVIAGQ
                     VGTGFLGTDAFQEVDLVGITQPITKWSYQIRRAEDVAWAVARAFYIAHSGRPGPVVLDF
                     AKNAQVEKTKYEPTKVDFIRSYVPVPDTDEETVQAAAELINNAERPLVLVGQGVELGNA
                     QSELREFIEKADMPAGCTLLGLSALPTEHPLNKGMLGMHGNLGPNINTNKCDVLIAVGM
                     RFDDRVTGNLATYAKQAKVIHFDIDPAEVNKNVKVDVAVLGDCKETLASVTKLLKKNTH
                     TEWIDSFKEYEKVEEEKVIRPELHPATNSLSMGEVVRAVSDATHHEAVLVTDVGQNQMI
                     SARYFKYTKERSIITSGGLGTMGFGLPAAIGATFGAPERTVCVFMGDGGLQMNIQELGT
                     IMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTPMLNPDYMKVASAYDIPSKRVFT
                     REELKEAIAEMLATDGPFLLEACVVEEGNVLPMTPPGGSVNQMLLEC"
     CDS             3293..3850
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg31_4"
                     /sec_met_domain="ALS_ss_C (E-value: 1.2e-19, bitscore:
                     59.1, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDIIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQENP
                     EASKLIRRYNARIVEVNPVFSIVEKNGMSEDITSLYGELKALNCVLQFVRSGRVAITTS
                     CFERVNEFLDGREAMYNQSKNQ"
     CDS             3856..4599
                     /locus_tag="ctg31_5"
                     /transl_table=11
                     /translation="MSEENRIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHANDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYENFSVQTWVENVYRLFTDRNFAIIDKDGK
                     KIGYARSVWAMINLNTRKPADLLTLHGGSIVDYVCDEPCPIEKPSRIKVTSDQPIATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYKTKRIRRFEMAYVAESYFGDELSFFCDEV
                     NANEFHVEVKKNGNEVVCRSKVIFE"
     CDS             4667..5710
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg31_6"
                     /sec_met_domain="IlvN (E-value: 1.6e-46, bitscore: 146.6,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 4e-42, bitscore: 132.7,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTTETVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQREGKTYDKAVADGWVPGETLFGIEEACEKGTIVMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWNDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATSKAYERTIALGIGIGSGYLFETTFQREATSDLTGERGSLMGAIQGL
                     LLAQYEVLRENGHSPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLYNSVKTGNEAQISIDSNSQPDYREKLNEELRQLRESEMWQTAVTVR
                     KLRPENN"
ORIGIN
        1 atatcgttgt gcctggagac atttcttcag cgacattctt tatcgtagca ggccttatca
       61 caccaaattc atgcattcgt ttaaagaacg ttggaatcaa cccaacaagg gatggaatct
      121 tacgtgtatg caaagatatg ggggctgatg ttacattgga aaatgtgatt gataatggtg
      181 gagaaccaac agcagatatc gttgtaaaga caagcagatt aaaaggaaca gtcattggtg
      241 gagaagttat cccaacactg atcgatgaga ttccagtcat tgccctttta gcagcatttg
      301 cagaaggtga gacagtgatc aaagatgcag cagaactgat cgtaaaaaat gcaaagttat
      361 attatgaaga aggagacgaa agtgtgcttc ctcgtagcat tgctacccgt caggctttct
      421 tgaatgccat gacgctggat atagcaatgg gtggttctac taatacagtt cttcacctgt
      481 tggctattgc tcatgaagca gaggtagatt tcaagatgga tgatatcgat atgctttccc
      541 gcaaggctcc ttgtctctgc aaggtggctc ccaatacaca gaaatatcat attcaggatg
      601 taaaccgtgc cggtggtatt attgctatca tggatgaact ggcaaaaggc ggtctgatag
      661 atacatctgt tcgacgggta gacggaatgt cattggcgga agctattaat gaatattcaa
      721 tcaccagccc gaatgtaagc gaaaaggcaa ttaaaaaata ttcgagtgca gccggaaata
      781 gattcaatct cgtacttggt tcacagggca tgtactataa ggaactggat aaagaccgtg
      841 caaacggatg tatccgtgat ttggaacatg cttatagcaa agacggtgga ttggccgtat
      901 tgaaaggcaa tattgcccaa gacggttgtg tggtaaaaac agcaggtgta gacgaaagta
      961 tctggaaatt taccggacct gccaaagtat ttgattcgca agaagcagcc tgtgaaggca
     1021 tcctcggagg ccgagtcgtc agtggcgatg tagtcgtcat tacgcacgaa ggtccgaagg
     1081 gtggtcccgg tatgcaggaa atgctttatc ctacctctta tatcaaatct cgtcatctcg
     1141 gaaaagaatg tgctttgatt accgacggac gtttcagtgg tggaacttcc ggattgagta
     1201 tcggacatat ttctcctgaa gcggcagctg gaggcaatat cggaaaaatt gtagacggag
     1261 acattatcga aatcgatatt ccggcccgga agattaacgt gcgactgacg gatgaagaac
     1321 tggcggcccg tccgatgact cctgtcactc gtgaccgtta tgtaccgaag agcctgaagg
     1381 cgtatgccag catggtaagc tctgccgata agggagcagt gagaattata taatatagtt
     1441 gataggtaat agttagctgc gcagcactta gcgcaatgat tgtaaaccat tcattttcaa
     1501 ctgtcatttc tcaactatca tttatcattt atcaactatc aactaatatg aaagatttaa
     1561 taacaggtgc agaggctttg atgcgctcct tggaacatca gggagtgact accatctttg
     1621 gttaccccgg tggttctatt atgccggtat tcgatgcctt gtacgatcat caaaatatat
     1681 tgaaccatat cttggttcgt cacgagcagg gagccgccca tgccgcacaa ggttatgccc
     1741 gtgtatcggg tgatgtaggt gtttgcctgg tgacgagcgg tcccggagca accaatacgg
     1801 taacaggcat tgcggatgcg atgatcgata gtacgcctat cgtggtgatt gccggacagg
     1861 taggaacagg ctttcttggg accgatgctt ttcaggaagt tgacttggta ggtattaccc
     1921 agccaatcac taaatggagt tatcagattc gccgtgcgga agatgtagcc tgggcggtag
     1981 cacgtgcgtt ttacattgcc catagtggtc gtccggggcc ggtggtactg gactttgcca
     2041 aaaatgcgca agtggagaaa acgaaatacg aaccaacgaa agttgatttt atccgtagtt
     2101 atgttcctgt tcccgatacg gacgaggaga ctgtacaggc tgctgccgaa ctgattaata
     2161 acgctgaacg tccgcttgta ttggttggac aaggtgttga actgggaaat gcacagagcg
     2221 aattgcgcga gttcatcgaa aaagcggata tgcctgcggg ttgtacgctg ctcggacttt
     2281 cggctttgcc gacagaacat ccgttgaata aaggtatgtt gggtatgcac ggcaatctgg
     2341 gaccgaatat caataccaat aaatgtgatg tgctgattgc tgtcggtatg cgttttgacg
     2401 accgtgtgac tggtaatttg gctacttatg ccaaacaggc aaaagtgatt cattttgata
     2461 tcgatcctgc ggaagttaat aagaatgtaa aggtagatgt agctgtattg ggagattgca
     2521 aggaaacact ggcttctgtg acgaagctcc tgaaaaagaa cactcatacc gaatggattg
     2581 atagcttcaa agaatatgag aaagtagagg aggaaaaagt aatccgtcct gaacttcatc
     2641 ctgccacaaa ctcgttgagt atgggggaag tggtacgtgc ggttagtgat gctactcacc
     2701 atgaagctgt tttggtaaca gatgtaggtc aaaatcagat gatatctgca cgttacttta
     2761 aatatacgaa agaacgcagt atcattactt ccggaggact tggaacaatg ggcttcgggc
     2821 ttcccgcagc tatcggggca actttcgggg caccggagcg taccgtatgc gtatttatgg
     2881 gagatggcgg actgcaaatg aatattcagg aactgggtac gattatggag cagaaggctc
     2941 cggtgaagat tatctgcctg aacaataatt acctcggaaa cgtacgtcag tggcaagcta
     3001 tgtttttcaa ccgtcgctat tcgtttaccc cgatgttgaa tccggattat atgaaagtgg
     3061 cctcggctta tgatattcct tccaagcgtg ttttcactcg tgaagaactg aaggaagcta
     3121 ttgctgaaat gctggcaacg gacggtccgt tcctgctcga agcttgcgtg gtggaagaag
     3181 gtaatgtttt gccgatgact cctccgggtg gttcggtaaa ccagatgttg ctggagtgct
     3241 aggagaagtt gagaatggag agttgagagt tcgcatttaa aaaattagag ttatgagtga
     3301 taaaacgtta tatacaatca ttgttcattc ggagaatata gctggattgc tgaatcaggt
     3361 aactgctgta tttacccgtc ggcaaataaa tattgagagt ctgaatgtat ctgcttcttc
     3421 tattaaaggt gtacataagt atacaataac agcctggact gataaggaca tcattgagaa
     3481 ggtagtgaaa cagatcgaaa agaagatcga cgtgattcag gcacattatt tcacagagga
     3541 tgagatttat ttccacgaga ttgcattata taaggtgtct actcctgctt ttcaggagaa
     3601 tccggaagca tccaagctga ttcgtagata caatgcacgt atagtggaag tcaatccggt
     3661 attttctatt gtcgagaaaa atggaatgag cgaagatata acttcgctct atggtgaact
     3721 gaaggctttg aattgtgttt tgcagtttgt tcgttcagga cgtgtagcga ttactaccag
     3781 ctgttttgaa cgggtgaatg agtttctcga tggacgggaa gctatgtata accaaagtaa
     3841 aaaccaataa gaataatgag tgaagaaaat agaataggga cttatcagtt tgtggcagaa
     3901 ccgttccatg ttgacttcaa cggacgtctg actatggggg tactgggaaa tcatctactg
     3961 aattgtgcag gttttcacgc caatgaccgt ggttttggta ttgcaacgct gaatgaggac
     4021 aattatacat gggtgctttc acgtctggct attgagttgg atgaaatgcc ttatcagtat
     4081 gagaatttct cggttcagac gtgggtggaa aatgtctacc gtttgtttac ggaccgtaat
     4141 tttgctatta ttgataagga tgggaagaag attggctacg cacgttccgt atgggcaatg
     4201 attaatctta atacccgcaa gcctgccgac ttgttgactt tgcatggggg aagtatcgta
     4261 gactatgtct gtgatgagcc ttgcccgatt gaaaagcctt cacgcatcaa agtgacaagc
     4321 gatcagccga tagctaccct gacggcaaaa tacagtgata tagatatcaa cggtcatgta
     4381 aacagtatcc gttacatcga gcatatcctt gatttgttcc cgattgaatt gtataagacc
     4441 aaacgtatcc gtcgttttga aatggcgtat gtggcggaaa gctattttgg tgatgaactc
     4501 tctttcttct gcgatgaagt gaatgcaaac gagtttcacg tagaagtgaa gaagaatggc
     4561 aacgaagttg tttgccgctc gaaagtgata tttgaataaa tgtgagaata taaaatcaat
     4621 taataacaga tggcgtaagt catcaaaaac taaaaacaaa aagaaaatgg cacagttgaa
     4681 ttttggaggt actactgaaa ctgtagtaat ccgtgacgaa tttccattgg aaaaagctcg
     4741 tgaagtattg aaaaatgaaa caatcgctgt aatcggttat ggcgtacagg gtcctggaca
     4801 ggcactgaac ttgcgtgata acggtttcaa tgtaatcgtt ggtcaacgtg aaggaaaaac
     4861 atacgataag gcagtagcag acggatgggt tccgggtgaa actttgttcg gtatcgaaga
     4921 agcttgtgag aaaggtacta ttgtaatgtg tctgttgtct gacgcagcag taatgtcagt
     4981 atggcctact atcaagcctt acctgactgc cggaaaagct ctttatttct cacacggttt
     5041 tgctatcact tggaacgacc gtacaggtgt agttcctcct gctgatatcg acgttatcat
     5101 ggttgctcct aaaggttcgg gtacatcttt gcgtactatg ttcctcgaag gacgcggctt
     5161 gaactcttct tatgctatct atcaggatgc tacaagcaag gcttacgaaa gaaccatcgc
     5221 actgggtatt ggtatcggtt ccggttatct gttcgaaaca actttccaac gtgaggctac
     5281 ttctgacctg acaggtgaac gtggttcatt gatgggggct atccaaggtt tgttgctggc
     5341 acagtacgaa gtattgcgtg aaaacggtca tagtccttct gaagcattca atgaaacagt
     5401 ggaagaactg acacagtcat tgatgccgtt attcgcaaag aacggtatgg actggatgta
     5461 cgctaactgc tctactactg ctcaacgtgg tgctctcgat tggatgggcc cattccacga
     5521 tgctatcaaa ccggtagttg aaaagttgta taatagcgtg aagactggta acgaagcaca
     5581 gatttctatt gatagtaatt cacaacctga ttatcgtgaa aagttaaacg aggagctccg
     5641 tcaattacgc gaaagcgaaa tgtggcagac tgctgtaact gttcgtaaac ttcgtccgga
     5701 gaataattaa ttagaccaga ttatacaagt tttaaaagaa ggagagagct ttttgctttc
     5761 tcctttttta ttaataagat atttaaaaat gggtaggtga agctt
//
