LOCUS       c00462_gnlPro..         7690 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BHKNFCMO_462.
ACCESSION   c00462_gnlPro..
VERSION     c00462_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:21:57
            Original ID  :: gnl|Prokka|BHKNFCMO_462
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7690
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7690
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[338:3852]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      339..3852
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7690
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7690
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(339..1094)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg462_1"
                     /sec_met_domain="Fer4_7 (E-value: 4.4e-13, bitscore: 38.5,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 3.1e-26, bitscore: 80.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.9e-07, bitscore: 20.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKVWRQAGPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQLISERKEPVVFDHDCREGICGMCSLYINGHPHGPATGATTCQIYMRRFNDGDTITVE
                     PWRSAGFPVIKDLMVDRTAYDKIMQAGGYVSVRTGAPQDANAILIAKPIADEAMDAASC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKPEALRRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNISISNIARLNRDFIIAKLKD"
     CDS             complement(1131..3110)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 202.3; E-value:
                     4.6e-62)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg462_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 4.4e-63, bitscore:
                     202.3, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5e-38, bitscore:
                     118.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKIDSKIPEGPVAEKWTNYKAHQKLVNPANKRRLDIIVVGTGLA
                     GASAAASLGEMGFRVFNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKG
                     GDYRAREANVYRLAEVSNAIIDQCVAQGVPFAREYGGTLDNRSFGGAQVSRTFYAKGQT
                     GQQLLLGAYSALSRQVNVGTVKLYTRYEMQDVVIVDGRARGIIAKNLVTGELERFAAHA
                     VVIATGGYGNAYFLSTNAMGCNCTAAISCYRKGAVFANPAYVQIHPTCIPVHGDKQSKL
                     TLMSESLRNDGRIWVPKKKEDAVKLQKGEIKGSDIPEEDRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSEAINRLGIDVVLQRYGNLFDMYEEITDVNPGE
                     LAKEISGVKYYNPMMIYPAIHYTMGGIWVDYELQTTIKGLFAIGECNFSDHGANRLGAS
                     ALMQGLADGYFVLPYTIQNYLADQITVPRFSTDLPEFAEAEKAVQAKIDKFMSIQGKES
                     VDSIHKKLGHVMWEYVGMGRTAEGLKKGIAELKEIRKEFETNLFIPGSKEGMNVELDKA
                     IRLYDFITMGELVAYDALNRNESCGGHFREEYQTEEGEAKRDDENFFYVACWEYQGDDE
                     KAPVLHKEPLVYEAIKVQTRNYKS"
     CDS             complement(3148..3852)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg462_3"
                     /sec_met_domain="SucD_FumR (E-value: 7.6e-132, bitscore:
                     425.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLSNSSVGRKVVMSVTGIALVLFLTFHMAMNLVAIISADGYNMI
                     CEFLGANWYALVATAGLAALFVIHIIYAFWLTMQNRKARGSERYAVVDKPKTVEWASQN
                     MLVLGLIVIVGLGLHLFNFWAKMQLPELMHNMGMHADTLTLAYAANGAYHIQKTFSSPV
                     YVVLYLVWLFALWFHLTHGFWSSMQSLGWNNKVWINRWKCISNIYSTIVVLGFALVVVV
                     FFVKTLICGGAC"
     CDS             complement(4064..4933)
                     /locus_tag="ctg462_4"
                     /transl_table=11
                     /translation="MPRDSFCIQYTNCTGCPKNVENILVYRNLPKGEHIPKDKCTQNCM
                     LFMIKGELLINSEEHPGITLRERQIVLQAIGSKVEILALTDVEYVVYWFNELPLLCEDR
                     YREMMEQAEAPLTYTPLIMSERLYHLVTSMPEFLAEENPCSKYIDLKCKELVFLITNFY
                     PLPQLSSFFYPISTYTESFHYFVMQNYSNVKNVEEFAHLGGYTTTTFRRLFKNLYGVPV
                     YEWILEKKREGILEDLQHTKMRITEICNRYGFDSLSHFAHFCKDSFGDTPRALRKKAAG
                     GEKIGKVQ"
     CDS             5207..6169
                     /locus_tag="ctg462_5"
                     /transl_table=11
                     /translation="MLMLRKIVFFFFLFVGLSLFAQQDYMVSSYVRGNFYNRDRISTES
                     LRASDDLIFLNVHPNKDGSLSFENPRAFQGKGVTTWEGLIKSVRAKVKGTKVKIRLGAS
                     SGEWKAMVADEAARTTFAKNIKTVLEKNKLDGIDLDFEWAENEKEYKDYSLAILKMREV
                     LGNKYLFSVSLHPVCYKISKEAIEAVDFISLQCYGPSPVRFPIEKYCSDIQMVLEYGIP
                     KEKLVAGVPFYGVTKDNSKKTEAYFNFVQNGLVTSPAQNEVIYQGDTYVFDGQDNIRIK
                     TRYAKEQKLKGMMSWDLATDLPLSDSRSLLKTMTEELRE"
     CDS             complement(6505..7689)
                     /locus_tag="ctg462_6"
                     /transl_table=11
                     /translation="KYTEQGRVTLSLYQTMRNEVAYTEIKVSDTGYGISAEALPHIFDR
                     YYQESGKHQASGTGIGLALVKNLVTLHEGEIRAESMQNEGSTFYISLLTDNIYPNALHA
                     DSTEPIQEDTNQEASLEYPQETTLDTGKPILLIVEDNEEIQKYIAESFADSFEVLTAYN
                     GEEGKQQALSRIPDIVVSDIMMPVMDGITLCRQLKEDVQTSHIPVILLTAKDSLQDKEE
                     GYEVGADSYLTKPFSASLLRSRINNLLESRKKLIAQFQTQSVPDNQADLKEKRIVIAEA
                     LSKLDNEFIEKITLLIEENLSSEKIDITYLSDKMCMSGSTLYRKMKALTGLSTNEYIRK
                     VKMQNAERLLLEGKFNISEIAYKVGMNSTGYFRQCFKEEFGVSPSDYLKQIIQS"
ORIGIN
        1 ggaactgctt cctgacagat gggaaaaggc taatgactaa aaagggatca ttagcctttt
       61 tattttaata cactatatag aatcgcggag acgcttacgc ggtaacgaat gattttacct
      121 tatttcaatc ctcgctttca cccgtaattc tctcattcat aacagttctg gctataaggt
      181 gaaatcggtg aaacttatga attaaaagtt catgttactg atccgtatta ataataattg
      241 tgatctactc atacataaag aaatcccctg tcggactagc cggcagggga ttctgtcatt
      301 aatcaaatta agctctcgct attgaaatct agcaatttct agtcttttaa ttttgcgatg
      361 atgaagtcgc ggttcaagcg agcgatgttg ctgatagaaa tatttttcgg acattcagct
      421 tcacaagcac gagtgtttgt acagttaccg aatcccagtt catccatctt tgacaacata
      481 gcttttgcac ggcgcaaagc ttccggttta ccttgcggca acaagttcaa ctggctaact
      541 tttgcagaaa cgaacaacat agcagaacca ttcttacatg cagctacaca agcaccgcaa
      601 ccgatacaag aagcagcatc catagcttcg tcagcgatag gtttcgcgat caggatagcg
      661 ttagcatcct gcggagcacc tgtgcgtacg cttacataac caccagcttg cataatcttg
      721 tcgtatgcag tacggtctac catcaagtct ttgatgacag ggaaaccagc cgaacgccaa
      781 ggttcaacag tgatggtatc accatcgttg aaacgacgca tatagatttg gcaagtagta
      841 gcacctgttg caggaccgtg cgggtgtccg ttgatgtaaa gagaacacat accgcagata
      901 ccttcgcggc agtcatgatc gaatactacc ggttcttttc tttcgctgat gagctgttcg
      961 ttcagaatat ccagcatttc gaggaaggaa gtatcaccgg ggatatcttt catttggtag
     1021 gtttcaaaag cacctttagc tttcggacca gcttggcgcc ataccttcag tgtaaatgat
     1081 atatttttat ccattttctt ctaattcttt taaatgttta acttcaccga ttagctcttg
     1141 tagttacgag tctgaacctt gatagcttcg tataccagcg gttctttgtg caataccgga
     1201 gccttttcat cgtcaccttg atattcccag catgctacat agaagaagtt ttcgtcatca
     1261 cgttttgctt ctccttcttc agtctggtat tcttcacgga agtgaccacc acaactttcg
     1321 ttacggttca atgcgtcgta agcaacaagc tcacccatag tgatgaagtc gtacagacgg
     1381 attgctttgt caagctctac gttcatacct tccttagaac cggggataaa caagttcgtt
     1441 tcgaattcct tgcggatttc tttcagttcg gcgatacctt tcttcaggcc ttctgccgta
     1501 cgtcccatac ctacatattc ccacatgaca tgacccagtt tcttgtggat agaatcaaca
     1561 gattctttac cctggatgct catgaacttg tcgatcttag cttgaactgc tttttcagct
     1621 tcagcaaatt caggaagatc ggtagagaaa cgaggaacag tgatctgatc tgccagatag
     1681 ttctggatag tgtaaggcaa tacgaagtaa ccgtcagcca aaccttgcat caatgcagaa
     1741 gcaccgaggc ggtttgcacc gtggtcggag aagttacatt caccgatagc gaacagaccc
     1801 ttgatagtgg tttgcagttc gtagtctacc cagataccac ccattgtata gtggatagcc
     1861 ggataaatca tcatcgggtt ataatatttc acaccactga tttctttagc cagttcgccc
     1921 ggattaacgt cagtgatttc ttcgtacata tcgaagaggt taccataacg ttgaagaaca
     1981 acgtcgatac ccaaacggtt gatagcttca gagaagtcga ggaatacggc caaacctgtg
     2041 ttgtttacac cgaaacctgc gtcgcaacgt tctttagcgg cacggctggc aacgtcacgc
     2101 ggaaccaggt taccgaatgc cggatagcgg cgttccaagt aatagtcgcg gtcttcttca
     2161 ggaatatcac ttcctttgat ttcgcctttc tgaagtttca cagcatcttc ttttttcttc
     2221 ggaacccaga tacgaccgtc gttacggaga gattcagaca tcaaagtcag tttagactgc
     2281 ttgtcaccgt gtaccgggat acaagtcggg tgaatctgaa cgtatgcagg gttagcgaat
     2341 acagcaccct tgcggtagca agagatagca gctgtacagt tacaacccat agcgttggta
     2401 gacaagaagt aagcgttacc ataaccaccg gtagcgataa ctactgcgtg agcggcgaag
     2461 cgttccagtt cacctgttac aagattttta gcgatgatac cgcgagcacg tccgtcaacg
     2521 atcaccacat cttgcatttc ataacgagtg tacagtttta cagtacctac gtttacctga
     2581 cggctcaatg cagagtaagc acccagcaac aactgctgac cagtctggcc tttagcgtag
     2641 aaagtacggg atacctgtgc accaccgaaa gaacggttgt ctagtgtacc accatattcg
     2701 cgagcgaaag gaacaccttg tgctacacat tggtcgatga tagcgttaga cacttcagct
     2761 agacgatata cgttagcttc acgggcacga tagtcaccac cttttacagt atcgtagaac
     2821 agacggtaaa cggagtcacc gtcattttga taattctttg cagcattgat accaccctgt
     2881 gcagcgatag agtgtgcacg acggggagaa tcctgaatac agaaattgaa tactctgaaa
     2941 cccatttcac caagtgaagc agcggcagaa gcacctgcca gacccgtacc cacaacgatg
     3001 atgtccaaac gacgtttatt agcggggttc accaatttct ggtgagcttt atagttggtc
     3061 catttctcag ccaccgggcc ttctggaatt tttgaatcta tcttgatcat aatattattt
     3121 acaatttaat catttacaat ttactattta gcaagcaccg ccacaaatca gtgttttcac
     3181 gaagaatacc actactacca gtgcgaaacc gagtacaaca atcgtagaat aaatgttaga
     3241 aatgcatttc caacgattga tccatacttt gttgttccat cccagtgatt gcatagagct
     3301 ccagaaaccg tgagtcaggt ggaaccacaa agcaaacagc caaacgaggt aaagaactac
     3361 gtaaaccggg ctagagaaag tcttctggat gtgataagca ccattagcgg cataagccag
     3421 cgtcagtgta tcagcgtgca tgcccatgtt gtgcatcagt tccggcaact gcatttttgc
     3481 ccagaagttg aagaggtgca aaccaagacc tacgataacg atcagaccca gcaccaacat
     3541 gttttgagat gcccattcta cagtttttgg tttgtcaacc actgcatagc gttcgctacc
     3601 acgcgctttg cgattctgca ttgtcagcca gaatgcgtag atgatgtgaa tcacaaaaag
     3661 agcggctagt cctgcggtag ccactaacgc ataccagttt gctcccagga actcacaaat
     3721 catgttgtaa ccatcagccg agatgattgc aaccaagttc atcgccatgt gaaatgttag
     3781 aaacaggaca agggcgatac cggtaacgct catcaccact ttccttccta cagatgaatt
     3841 acttaaccac ataaatgatt gaatttaaat tatttaattg ttagaactaa aatttcgacg
     3901 ttttacacct cttacagact gcaaaaatag gggaaataga ttgattgagc aaggaattaa
     3961 agaaataatt ctttaataat ggatcaatgc gccaatgtgt caatatgcca gccagtatgt
     4021 acgcatggtg caactgattg gcacattagt agaatgaggc tgtttattgt acttttccga
     4081 ttttctcacc gccggctgct ttcttgcgta aagcgcgtgg ggtatctccg aaagagtctt
     4141 tacaaaagtg ggcgaaatga gacagggagt cgaatccgta tctgttacag atttcagtga
     4201 ttcgcatttt ggtgtgctgc aggtcttcca ggattccttc ccgtttcttt tccagtatcc
     4261 attcgtatac gggcacacca tagaggtttt tgaaaaggcg gcggaaagta gtggtcgtat
     4321 atcctcccag atgcgcaaat tcttccacgt tcttcacgtt gctgtaattc tgcatgacga
     4381 aatagtggaa actttccgta taagtgctga tcggatagaa gaatgaactc agttggggca
     4441 gcggatagaa gttggtaatc aagaaaacca gttctttgca ttttaagtcg atatacttgc
     4501 tgcaagggtt ttcctcagcg aggaattcgg gcatactggt gacaaggtgg taaagccttt
     4561 cgctcataat cagtggagtg taggtcaaag gagcttccgc ctgttccatc atttccctat
     4621 atcggtcttc gcaaagcaaa ggcagttcgt tgaaccagta gacaacgtat tcaacatctg
     4681 tcagtgccag tatttcgacc ttggagccaa tagcttgcaa aacaatttgt ctctcgcgta
     4741 acgttattcc gggatgctct tcgctattga taagtaattc gccctttatc ataaaaagca
     4801 tacaattctg cgtacactta tccttgggga tatgctctcc tttaggtaag tttctatata
     4861 caaggatatt ctcaacattt tttggacatc ccgtacagtt ggtatattgt atgcaaaatg
     4921 aatctcttgg catgagtttt atgcgtgatt tattatctta acggaacaaa gataggcttt
     4981 ttttgcggaa aatggaactc tttcttttat ttatgtttta gtagaaacct gcaaatatta
     5041 ttcttatccc tttggatgat gttagagaat tcaatattta tctgttgtaa atttctgaat
     5101 agaaaacttt atgttgattt ttggaagtat tatagttgtt tttgaacatc tgtttccctt
     5161 tgatatgcgt atttttgcaa tctaaacgtt ttaattatca atcattatgc taatgctaag
     5221 aaaaattgta ttcttctttt ttttgtttgt tggattatca ttgtttgcac agcaagacta
     5281 tatggtcagt tcttatgtgc ggggtaattt ttacaatcgt gatcgtatct ctacagaaag
     5341 cttgcgggca agtgacgacc ttattttctt gaatgttcat cctaataaag acggatcatt
     5401 gtcatttgag aatccacgtg catttcaggg aaaaggggtg accacttggg aaggtttgat
     5461 taaatcggtt cgtgcaaaag taaaaggtac aaaagtgaaa atacgtttgg gagcttccag
     5521 tggcgaatgg aaagcgatgg tggccgatga ggccgctcgt acgacgtttg caaagaacat
     5581 aaagactgta ctggaaaaga ataaactgga tggcattgat cttgattttg agtgggcaga
     5641 aaatgagaaa gaatataaag attatagttt ggctatcctg aagatgcggg aagtgttggg
     5701 aaacaagtat cttttcagtg tatctctgca tcctgtttgt tataagatta gtaaggaagc
     5761 tattgaagct gttgatttta tttcacttca gtgctatggt ccttcaccgg ttcgttttcc
     5821 gatagagaaa tattgttcgg atatacagat ggtattggag tatggtattc cgaaggaaaa
     5881 actggtggca ggagttcctt tttatggggt gaccaaagat aattcaaaga agacagaggc
     5941 ttatttcaat tttgtacaga atggattggt tacgagtcct gcacagaatg aagtgatcta
     6001 tcagggagat acttatgtgt ttgacggtca ggataacatt cgtatcaaaa cccgttatgc
     6061 aaaagaacag aagctgaaag gtatgatgag ttgggattta gctacggatt tgccgttaag
     6121 tgatagcagg tcattactaa agacaatgac tgaagaattg agggaatgac ttagttcgca
     6181 cgactactgc ttgtaatgtt ttctttttgt agatgctgtt taacaggctc tgttttaata
     6241 ggttcctccc ctcctttatt ataaatcaga tttataaaga atgtgttaaa acgtttttct
     6301 ttagagaaat acccctgccg catcgtttga agtggcccca aaaaagttgg acggtttata
     6361 aattagcaag atagataaag agttcggtgt tagcaccgga ctctttcctt ttaatttagg
     6421 cattatgact ttaccctctt ctttttcggc aggagcaaaa tttgtttctc aaaacgtatt
     6481 aagtgtaaat ggctctctcc ctttttatga ttgtatgatc tgcttcaggt aatccgaagg
     6541 cgagacgcca aattcctctt taaagcattg acggaaatac cccgtactgt tcattcctac
     6601 tttataggcg atttcagaga tgttgaactt cccttcaagc aataaacgtt ctgcattttg
     6661 cattttcact ttccggatat attcgttggt cgataatcct gtcagtgctt tcattttccg
     6721 gtagagggtg gaaccgctca tacacatctt gtctgaaaga taggtaatat caatcttctc
     6781 cgaagagagg ttttcttcga ttaaaagggt gattttttca ataaattcat tatccagctt
     6841 gcttagagct tcggcgatta cgatacgttt ttcttttaaa tctgcctgat tgtccggaac
     6901 ggattgtgtc tggaattgag ctatcagttt ctttcttgat tccagcagat tgttgatacg
     6961 gctgcgcagt agagaagcac tgaaaggctt tgtcagataa gaatcggctc ctacctcata
     7021 tccttcttct ttgtcttgca gagaatcctt ggctgtcaat aggatgacag gaatatgact
     7081 ggtttgtaca tcctctttta attggcgaca tagagtgata ccgtccatga caggcatcat
     7141 aatatcgctg actacaatat ccgggatgcg gctaagagct tgttgcttcc cttcttctcc
     7201 attgtaggcg gtgagtactt cgaacgaatc ggcaaaggac tcggcgatat atttctgtat
     7261 ctcttcattg tcttctacaa tcaacaggat cggtttaccg gtatccagtg tggtttcttg
     7321 tggatattca aggcttgctt cctgattcgt atcttcttga atcggttctg tagaatctgc
     7381 gtgtaaggca ttgggataga tattatccgt caataggctg atatagaaag tacttccttc
     7441 gttttgcata ctttctgccc ggatttctcc ttcatgcagg gtgaccagat tcttcactag
     7501 ggccaggcca atgcctgttc cggatgcctg atgcttgccg ctttcctgat aataacggtc
     7561 aaaaatatgt ggcagggctt cagcagaaat tccatatccc gtgtcactga cttttatctc
     7621 cgtataagca acttcattgc gcatcgtttg atacaaactt agagtgacgc gtccctgttc
     7681 cgtatatttg
//
