LOCUS       gnlProkkaHFOGJAFA_268  14562 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HFOGJAFA_268.
ACCESSION   gnlProkkaHFOGJAFA_268
VERSION     gnlProkkaHFOGJAFA_268
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 10:01:39
            Original ID  :: gnl|Prokka|HFOGJAFA_268
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 20219
            Orig. end    :: 34781
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..14562
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:14096]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..14096
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..14562
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..14562
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(483..1733)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg268_22"
                     /sec_met_domain="Polysacc_synt (E-value: 4.6e-40, bitscore:
                     129.1, seeds: 31, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVIIDKILSNKILVKNYFFVSILNVLALFLPLITYPYIISKLG
                     DELYGYIVFSFTVITYIEMVAAWGFDISAVKDVAQYQHVKHRLNEIVSSVLYIRFIFCM
                     FLLSLLVGALMLFDVKMKMLYILFSGHCIASVLFPGWFFQGIQELKYVTYFQLICKLVF
                     VILMVVFIHSKADYLYYPILFSLGSLFGTAYGLYVMFEKYKVRLIGVKMRNVVYRFKRS
                     TSIFYSRIADMIIERSNAILLGNFIGMQEVAYYDLANKIVRLGSFPIMILNQVLYPKVA
                     SERNFRLMRKVMAISFWVAILIWGLCVLLAPWGVELLGKGLMEPSVEIVYILSPLIVTN
                     SIIYLQGSPILVAAGYFKAFNITMWCSLGVYVACMLFVFIFNLHTNLLSILIIQLITNI
                     SLLVARAYYIRKFKILR"
     CDS             complement(1753..2727)
                     /locus_tag="ctg268_23"
                     /transl_table=11
                     /translation="MFRSYFSRVLKESFLLLSLYNTYYRLRNKKRFWKEINRRQALSMF
                     DYKELVKPIPYYPIEAIKDSNFYGQAYALKQYSGVNKFGWSIEHGLYVDDYVPMAAWCK
                     TTKRIMTFSNIRVKGLGSLHKPVIAIGPYIHYAECMLNSEEMNSLKKELGKTLLFFPTH
                     TCCEGGLEYEIHCMIDELLELKEKLGFDTVIVNMYYLDENKNGFGDLYNKAGFKVTTAG
                     HQLDINFLNRLKTIILLSDYTCSNSIGTHTGYCVYLGKPHLVINPVQTLEEVNPLWRDL
                     WETFEKDSSQAQVDKRLLASKYWGFDCIKSREEMRALLINNEC"
     CDS             complement(2750..3826)
                     /locus_tag="ctg268_24"
                     /transl_table=11
                     /translation="MGIYYFLLWIGVIGTFLSQDSRLKRTGFYIIFIYILALFVMVVFR
                     YDVGTDYLEYTDYYYRIHSLFELTSEDFFVEPGYVLLSSLLRSIGAPFELLSFILFLII
                     VCNLKRAIAFFSDNIPLSVVLYVFLFFLSFHFNLIRHGVMVSFVWKGYSWWFVGKKKRA
                     FISLVCGAMFHALSLCFLSLLFIHLRKYPIYIYAGVLVFSFIISAHPDWLLSLFDTLLS
                     SIIGTDNRLFFYLNGGHSGVLNETGVTIGMFFNLTLFCVSYFLLIDKKSIFLYNILFIG
                     ITFFLLFNSFGAISERIASTCYVANIFILPSVLNLMYVNKWRFLCLAVILLYGILMFNK
                     EVSKESANYIPFTTIFSM"
     CDS             complement(3871..4761)
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1079:oxidoreductase (Score: 114.4; E-value: 1.2e-34)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg268_25"
                     /transl_table=11
                     /translation="MNSASVSVAYNPNQEECVSFCEKFLIPIVARDLEELYENCDAVYI
                     ASPHYTHYEYAKSALNAGKSVLCETPFVFSKKQAEELYSIAEKKGLLLQIALKTAYCPA
                     FGHLFSLLNSGVIGEIVEVNASVTTLTDENSAKLDSKYFGGSMSENACFPLLPIFKFLG
                     TKFRNINFYSKMKNDVDMFTKAVFRYDHAVASFQVGLGVKTEGSLTIAGTKGYVYVPAP
                     WWKTDYFEVRYEDQNYNKKYFYPYVGEGLRYEIKDFVVAILTDGYYFSKVSKEENLMMA
                     EVQEIYILGKNVYKL"
     CDS             complement(4758..4973)
                     /locus_tag="ctg268_26"
                     /transl_table=11
                     /translation="MIYRNMEWMFLQSVQIGKAILTISMSSVKSFTFLALRVSHLLCFA
                     RSVLLLILALLVLAVLLHVLFQSRKM"
     CDS             complement(5001..5177)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg268_27"
                     /sec_met_domain="CTP_transf_like (E-value: 9.5e-11,
                     bitscore: 33.5, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFHQGHYNLLKRAKALGDYLIVGVTTDNFDLERGKMNTCNNVMER
                     IEAVKSTGLADQL"
     CDS             complement(5235..5495)
                     /locus_tag="ctg268_28"
                     /transl_table=11
                     /translation="MKNVINMDITTKIKRSKLLYYVYYYVMSGLVNLYKLFLKPDGKLM
                     LFVCYGGRHYSDSTRVIYEAMLKDERFKDYIGLRQTDCCIY"
     CDS             complement(5820..7007)
                     /locus_tag="ctg268_29"
                     /transl_table=11
                     /translation="MEILNRKLSLLKGKVENSQPITIMIIGLGSVGCYLLDYLVSLGDS
                     QLRLVVVGRNAEKMQMDINIIRTASTIRHQCRSEIKVVDNCDLNDVNSIAAVLEAEKPD
                     FIVNSSRVYSGLKYGSISWSNLRAYGIWTPLSIRYAKNIMEAYDKANCEAISINTSYSD
                     AVIPWLKSAGKAYFDFGSGNLNHLVPRIKFYIAEKYGIKNFNDIDVTIAVSHFHDVVIS
                     KEGHAEGQDILLDIKFQGKDMDFNKEELLKSCSIAMPVDQKRNMMNASSNFDIIFSVLT
                     ALREEKQVKIHTPGVNGEIGGYPIIIDGVTATAKFDESVWTIDQMRKANRESIYCDGVE
                     NITDATLVYTDELVAKVKKSFNVDLPKSVKFVDIENVADLIINQIIKPQVFVFVQ"
     CDS             complement(6995..8002)
                     /locus_tag="ctg268_30"
                     /transl_table=11
                     /translation="MAVLERDYRIDGIKWLLIVLVTFGHVIEPALSNPIANKLYSIIYI
                     FHMPLFVFISGYYANVKDKEKLISKGFMLLETFLVVMIPQCFYYGSIIPLLNPENSGWY
                     LISLITWYIIFLLIINKLVGKIVVQYNGCGGQLLIFSCLLAVFAYMLPLKQFGNLLSFQ
                     RSCMFLPFFMMGFLRRVKGLPIIPDVSKCIKLVLAIASLVCFAFCLIYSGRILHVLEFN
                     NANVWHMSNLYNQNIGMLLVLKVLVYIGSIITTFAVLMFVNLPKSICAYGSKTLAYYVI
                     QGMFAHILSTYLQVNFYQALFLCVCILILTTVLLRFVDSKFITNPFSTLLKWKY"
     CDS             complement(8011..8751)
                     /locus_tag="ctg268_31"
                     /transl_table=11
                     /translation="MKYLIITVAGTATRFNRDTKEETLKCLYYTDEPQYALLAQLLKNC
                     GEYDKYILVGGYLYEKLGRFVKNELSGYGKKIELVYNEHFKDYGSGYSLYKGIEAIKEA
                     GDVTFVEGDLFFMATDFRQVYNSPKSVITINREPIYSNKAVALYINVDGKPRYLYDTNH
                     QTLTVSEPFKAIFNSGQMWKFSSSERLLKTVDSLTKEQLQGTNLEIIQAYFSDMNSEEY
                     DVVTFTDWFNCNTVADYDIVRKLM"
     CDS             complement(8748..9014)
                     /locus_tag="ctg268_32"
                     /transl_table=11
                     /translation="MELIASEDALDGTVYNVATEEEIAIIDLVKTIAIKMGINDVDIRF
                     EGYRKSDPERRLLNTDKIRTRTSWKPIITLDEGLTMCVNSIKK"
     CDS             9039..9281
                     /locus_tag="ctg268_33"
                     /transl_table=11
                     /translation="MKEGTFVHLRVFKKGYGMAFKQIINDMRDNVLCKTLVPAIYISKA
                     NRTTFNSSLANSIFHKEFSHQLAASITCTLAMFRC"
     CDS             9456..9734
                     /locus_tag="ctg268_34"
                     /transl_table=11
                     /translation="MLCMDNSSTIDKHQTTIALNFEFFRLIAYFIHLSDFVNVVIIEDE
                     RGIVNNVFNGINRTCKEVVETDYRMTLRNKLTRQFTTRHASNTTYYI"
     CDS             complement(9744..10472)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Metallophos_2"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg268_35"
                     /sec_met_domain="Metallophos_2 (E-value: 1.6e-27, bitscore:
                     87.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVILFADIHANLSAFEAVLADIEKHYQPDAMISLGDFIDYGMRS
                     NEIIERIQTVNIPLLANLKGNHEKALIDGELSCFSSERARVMSKYTRNHLTQTSIDYIS
                     NVMQEPMQELEIGGKKVLLLHGTIDDPYWGKLTPAKVEDEKYQDFDYVLSGHIHQPFKI
                     DVFFKVDNPIMRNQKRTTFINPGSVGQPRNQNPYAQYVYINFETGETHFNAVPYDIEAE
                     QSLFPKEIDTFYSERLKFGI"
     CDS             complement(10476..11603)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 74.7;
                     E-value: 1.1e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg268_36"
                     /sec_met_domain="TPP_enzyme_N (E-value: 3.3e-20, bitscore:
                     63.8, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.3e-23, bitscore:
                     74.9, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRPEFFVNILKEQGIDFYAGVPDSLLKNICAYITDHLPAEQNII
                     AANEGGAMGIAVGYHLATGKVPVVYMQNSGEGNIINPFASLTDKDVYNIPVLLVIGWRG
                     RPGVHDEPQHVKQGKVTIGLLNTMGINYAILPKDEEGAAKQIKIAVDFMKATNECYALV
                     IEKDTFDTYKLQSVEVNELAMSREETIQKVASNIEDNACIVSTTGMISRELFEARTAWS
                     QGHERDFLTVGAMGHASQIALGIALQKPERRVYCFDGDGASIMHMGNMAITASMKCKNY
                     VHIVFNNGAHDSVGGQPTVGLKIDFCAIAQAVGYKAAYSVETMEALEAKLAEFKNAEGP
                     VFLQICVKKGNRKDLGRPTTTPIQNKEAFMEFLNK"
     CDS             complement(11638..12948)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 190.9;
                     E-value: 4.6e-58)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg268_37"
                     /sec_met_domain="CTP_transf_like (E-value: 4.1e-12,
                     bitscore: 37.9, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 4.7e-114, bitscore:
                     371.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 2.9e-61, bitscore:
                     198.3, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTVYLGMIGDIMHPGYINIINKATEYGDVIIGLFTDKAIANHR
                     RLPYLTWEQRKNVVESIRNVSRVVPQDDWSYVSNLLKYKPDYIIHGDDWQVGPDKYIRD
                     EVFKVMEKLGGEVIEIPYTQNISASGIKQEIDALGVTPQMRLSSLRRLIAAKPIVRILE
                     SHNGLTGLIAEHTKVEVNGQEREFDGMWASSLTDSTSKGKPDIEAVDLTTRLHDLNDTL
                     EVTTKPVIFDGDTGGKIEHFGFTVRTLERLGISCVIIEDKVGLKQNSLFGTDAVQTQDT
                     IEGFQAKIRAGKEAQITRDFMIVSRCESLIAGKTVDDALERCHAYVEAGTDGIMIHSKE
                     KSGEDIKEFCLRFREKNAHTPIIVVPTTYNQFTEEELAEWGINVVIYANHMLRSAYPAM
                     VKCAETILYTSRSLEASEQYCMPIKQILNLIPGTKKK"
     CDS             complement(12973..13347)
                     /locus_tag="ctg268_38"
                     /transl_table=11
                     /translation="MTYRQVGTNSFTVKYYVEKFILDMNTMKIIRVDEYRDKKKINRPA
                     GSLFSVDGEIYRVAQKCSRAYGESIFVYKTSKNFDFIKDKKVAELTGQSIVLSDGRKPI
                     LLHTYSQAGGIEVIDYRCSL"
     CDS             complement(13344..14096)
                     /NRPS_PKS="Domain: Aminotran_5 (3-189). E-value: 1.1e-20.
                     Score: 65.7. Matches aSDomain:
                     nrpspksdomains_ctg268_39_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg268_39"
                     /sec_met_domain="Aminotran_5 (E-value: 3.5e-18, bitscore:
                     57.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMLENERLVKKFANATKDSKVVFMTCSGSGGMEAAIINCLTPQDK
                     ALVINGGSFGERFVELLTLHKIPFTEIKLKYGRALKPEHLAEYESKGYTTFLMQKHETF
                     TGVHYDINLVSDFCKRNNCFLIVDIISTFLCDSFDMVAMDVGVMITGSQKALACAPGIA
                     VMLLAPSAIKRIEKVQCCCQYLDLKLALKNMERGQTPCTPAVGILRQINVRLKEIESAG
                     GAEVEIARCAELAKYFRDKLVKNNLPLF"
     aSDomain        complement(13530..14087)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg268_39_Aminotran_5.1"
                     /evalue="1.10E-20"
                     /label="ctg268_39_Aminotran_5.1"
                     /locus_tag="ctg268_39"
                     /protein_end="189"
                     /protein_start="3"
                     /score="65.7"
                     /tool="antismash"
                     /translation="ENERLVKKFANATKDSKVVFMTCSGSGGMEAAIINCLTPQDKALV
                     INGGSFGERFVELLTLHKIPFTEIKLKYGRALKPEHLAEYESKGYTTFLMQKHETFTGV
                     HYDINLVSDFCKRNNCFLIVDIISTFLCDSFDMVAMDVGVMITGSQKALACAPGIAVML
                     LAPSAIKRIEKVQCCCQYLDLKL"
     CDS             complement(14093..14194)
                     /locus_tag="ctg268_40"
                     /transl_table=11
                     /translation="MINFTVGPVQSSDAVRVIGAEQVPYLEQQSFQK"
ORIGIN
        1 caaaaaagct ccatgtctta ctactgaata attggtatcg tgaagaaata atttcagatg
       61 cgattgccac catgaatgtt ttacataatc aaaagggaaa atatctaaaa atataccttt
      121 atgacatttc aatcgttcat acattggctc atgaaaagta ctgtatttat cacgtaattt
      181 cgcaaaaaac atgggataat cagaatctgt ttctctggtt tgcaggtaga tatcatcgcg
      241 taattcagtt tttgcaactt ctaaaaaacg ataataatca ttacgcaaca tcgctacatc
      301 aatatcatcg tcccaaggaa taaatccacc atgacgaata gctcctaaca aagttcctcc
      361 atcaagccaa taaggaatat catattttgt acaaatgcga tctaactctc taagtaattt
      421 aagcataata agtctggctt gctcattcac attcttacct ttttcacggt catctttcag
      481 cattatctta atattttaaa ttttctaatg taatatgctc ttgccactaa taatgaaata
      541 ttagttataa gctgtataat caaaattgat aaaagattgg tatgcaagtt gaaaataaat
      601 acaaataaca tgcatgcaac gtaaaccccc aaagagcacc acatggttat gttaaatgct
      661 ttaaaatagc cagctgcaac caagatggga ctaccttgca aataaatgat agaattggta
      721 actatcagcg gagaaagaat atatactatt tcaacagagg gttccatcaa ccctttgccc
      781 aaaagttcaa ctccccaagg tgccagcagc acacacagcc cccatatcag tatagcaacc
      841 caaaaagata ttgccataac cttacgcatt aatcgaaaat tacgttcaga tgccacttta
      901 ggatagagaa cttgattaag aatcattata ggaaaagaac ctaaacgaac tatcttatta
      961 gctaagtcat aatatgccac ttcctgcata ccaataaaat tgccaagcaa aatagcattg
     1021 cttcgttcta ttatcatatc agcaatacgt gaataaaaaa tggaagtaga gcgtttgaaa
     1081 cgatacacca cattgcgcat ttttacccct atcagcctaa ccttatattt ctcaaacatc
     1141 acatacaatc cataagcagt gccaaacaag ctaccaagac tgaacaaaat aggataatac
     1201 agatagtcgg ctttcgaatg aatgaacaca accatcaata tcacaaaaac aagtttacaa
     1261 atcaactgaa aataggtaac atacttcagt tcttgtattc cttgaaaaaa ccaacccggg
     1321 aataatacag aagctatgca atgtcctgaa aaaaggatat aaagcatttt catctttaca
     1381 tcaaataaca ttaatgctcc cactaatagc gacagcaaaa acatacaaaa tataaagcga
     1441 atgtaaagca ccgatgatac aatctcattc aatctgtgct tgacatgttg atattgggct
     1501 acatctttca ccgcagagat atcaaatccc catgcagcca ccatttcaat atatgtaatt
     1561 accgtaaacg aaaatacaat gtagccatat aattcatcac ccagtttact aataatataa
     1621 ggataagtaa ttaatggtaa aaacaaagct aatacattaa gtatagaaac aaaaaaatag
     1681 ttctttacca agattttatt tgataatatt ttatctatta ttacccgttt caactttacc
     1741 tatttaagat attcaacatt cattatttat taatagagcc ctcatttctt ccctactttt
     1801 aatacaatca aatccccaat atttggaggc aagaagtcgt ttatccactt gtgcttgtga
     1861 tgaatctttt tcaaaagttt cccataaatc acgccataaa ggattcactt cttccaaggt
     1921 ttgtacagga ttaattacaa gatgaggttt gccgagatat acgcaatatc ctgtatgagt
     1981 accaatacta tttgaacaag tataatcaga taataagata atcgttttca gacgattcaa
     2041 gaagtttata tccaactgat gaccagcagt ggtcacttta aatccagctt tattatataa
     2101 atcaccaaaa ccgtttttat tttcatctaa ataatacata ttcacaatta cagtatcaaa
     2161 tccaagcttc tctttaagtt ctagcaattc atcaatcata caatgtattt catattctaa
     2221 gccaccctca cagcatgtat gtgtaggaaa gaaaagaaga gttttgccta attccttttt
     2281 caaggaattc atctcttcac tattcaacat acattcagca taatgtatgt aagggccaat
     2341 agcaatcacg ggtttatgta aacttccaag ccccttcact cgtatgttgc tgaaagtcat
     2401 gatacgtttg gtcgttttac accacgcggc cataggcaca taatcatcta cataaagtcc
     2461 atgttctatt gaccatccaa acttatttac accagagtat tgtttcaaag cataagcctg
     2521 accataaaaa ttagaatcct taatagcctc tattggataa taaggtatag gttttactaa
     2581 ttccttataa tcaaacatgc tcaatgcctg acgacggtta atctctttcc aaaaccgttt
     2641 cttgtttctc aggcgataat aagtgttata gagtgacaat aaaaggaatg attctttaag
     2701 cactcgtgaa aagtaagaac gaaacatttt gcaaaatcct tcaattaggt tacattgaaa
     2761 aaatcgtagt aaaaggtata taattagctg attcctttga cacttctttg ttgaacatta
     2821 aaattccata caaaagaata acagctaaac acaaaaaacg ccatttatta acatacatca
     2881 gatttaaaac agatgggaga ataaaaatat ttgctacata acatgtgctt gctatacgtt
     2941 ctgaaatagc accaaaagaa ttgaataata aaaaaaaagt aatacctata aataagatat
     3001 tatagagaaa tatacttttt ttgtctataa gtagaaaata agaaacacaa aaaagagtga
     3061 gattaaagaa cattcctatc gtaacccctg tctcattcaa aactcctgaa tgtcccccat
     3121 ttaaataaaa aaacaatcta ttgtcagtac ctattataga agaaagcaac gtatcaaata
     3181 gagaaaggag ccaatcggga tgagcagata taataaaaga aaacaccaaa acacctgcat
     3241 atatatagat gggatatttt cttaaatgga taaacaataa tgataagaag caaagactta
     3301 acgcatgaaa catggcacca caaacaagag aaataaatgc tcttttcttt ttaccgacaa
     3361 accaccagga ataccctttc caaacaaagc tcaccatcac cccatgacga atcaagttaa
     3421 aatggaaaga taaaaagaat agaaatacat ataatacgac tgacaaaggt atattatctg
     3481 aaaaaaaagc aatggctctt tttagattac aaactattat caaaaaaaga ataaatgaaa
     3541 ggagttcaaa tggagcacca atagaacgca acaaggagga taacagcaca taaccaggtt
     3601 ccacaaaaaa atcttcactc gtcaattcaa acaaagaatg aattcgataa taataatccg
     3661 tatattccaa gtaatcagtt cctacgtcat acctaaatac taccattacg aaaagtgcaa
     3721 gaatatatat gaaaataata taaaaccccg ttctcttcaa tctgctatcc tgactcagaa
     3781 atgttcctat aacacctatc cacaataaaa aataataaat ccccatttac ttgattacaa
     3841 attaactgcc ctaacttgtc aataacaata ctacaacttg tacacgttct tccccaatat
     3901 gtatatttcc tgaacctctg ccatcataag gttctcttcc ttcgacacct tactaaagta
     3961 ataaccatcc gttaagattg cgactacaaa gtcttttatt tcataacgta aaccttcacc
     4021 tacatatgga taaaaatact tcttattata attttggtct tcataacgaa cctcaaagta
     4081 atcagtcttc caccatggag ctggtacata cacatacccc ttggttcctg caatagtcaa
     4141 agaaccttct gtcttaacgc ccaagcctac ttgaaatgaa gcaacggcat gatcataacg
     4201 aaaaacagcc ttagtgaaca tatccacatc attcttcatc ttggaataaa aattaatgtt
     4261 tcggaacttt gttcccaaaa acttaaagat aggcaacaga ggaaaacaag cattttcact
     4321 catactaccg ccaaagtatt tgctgtcaag ctttgctgaa ttttcgtctg taagagtggt
     4381 cacagaagca tttacctcaa cgatctcacc aatcacaccg gagttgagca gagagaataa
     4441 gtgcccgaaa gcaggacaat aagcagtttt gagtgcgatc tgaagcaata accctttttt
     4501 ctctgcaatc gagtagagtt cttctgcctg cttcttacta aatacaaatg gagtttcaca
     4561 aagaacagac tttcccgcgt tcaaagcaga ctttgcatat tcataatgag tatagtgtgg
     4621 agatgcaata taaacggcat cgcaattttc atacaactct tctaaatcac gagcaactat
     4681 aggaatcaag aatttttcac agaacgaaac acattcttct tgattaggat tgtatgcaac
     4741 agaaacggac gcactattca catttttcgg ctctggaaca aaacgtgaag caatactgcc
     4801 agtaccaata atgccaatat taacaatagg acgcttctgg cgaagcaaag tagatgagac
     4861 accctgagtg cgaggaaggt aaatgacttt acagaactca ttgagatagt caaaatagcc
     4921 ttcccaatct gaaccgattg caaaaacatc cactccatat ttctgtatat catcaatctt
     4981 ctgcccacga tattcttcta tcacaattga tctgcaagac cagtggattt aaccgcttca
     5041 atacgttcca tcacgttatt gcacgtgttc atcttgcctc tctccaagtc gaaattatct
     5101 gtggtaacac ccacaatgag gtaatcacct aaagctttcg cacgttttaa gaggttataa
     5161 tgcccctgat gaaacaaatc gtatgttcct aataactttc ttcataacct tagataaatc
     5221 gcttttctct taaatcaata aatgcagcaa tcagtttgtc gtaatcctat atagtccttg
     5281 aaacgttcat ccttcaacat ggcctcataa atcactcgcg tactatcgga ataatgacgt
     5341 ccaccataac atacaaaaag catgagttta ccgtctggct tcaagaacaa cttgtacaaa
     5401 tttaccaaac ctgacattac gtaatagtag acgtaataaa gtaatttact tctttttatc
     5461 tttgttgtga tatccatatt tattacattt ttcatttgac aatcggagat atagaactgt
     5521 aattatttta ttacgcgtat ccttatacca cttatgaaaa atattatagg aactttatgc
     5581 ttcatacaaa aggactggag gaacttgaca tgaggtaaag ccttggtctg gtactgaaaa
     5641 taaaaacttg ctaaatcttc gctgctatac atatcttgct aattttaatt tacaacgaag
     5701 gctaacattt cattttgaac tttttaaagt gcttacatag ctgtgtatat cataaaacta
     5761 taagttaaag attatacaga aaattaacaa caatctatta tctgccatta gtttacaaat
     5821 tattgcacaa aaacaaacac ttgaggttta attatctgat tgataattaa atctgctaca
     5881 ttttcaatat ccacaaactt taccgactta ggcaaatcaa cattaaacga cttctttacc
     5941 ttagctacaa gttcatcagt atagacgaga gtagcgtcag ttatattctc tactccatca
     6001 caataaattg actcacggtt agctttacgc atctggtcta tagtccatac agactcatca
     6061 aacttggcag tagcagttac tccatcaata attataggat aaccaccaat ttcaccattt
     6121 accccaggag tatgtatttt gacttgtttt tcctctcgaa gagcagtaag cacagagaat
     6181 atgatatcaa aattactgga agcattcatc atattgcgct tctgatctac aggcattgct
     6241 atagaacagg atttaagaag ttcttcctta ttaaaatcca tatccttgcc ttgaaacttt
     6301 atatcaagca agatgtcctg accttctgca tgaccttcct tagaaatgac aacatcatga
     6361 aaatgtgata ccgcaatagt tacatcaatg tcattaaaat tctttatacc atatttctct
     6421 gcaatataga attttatacg aggcacaaga tgattcaaat taccactacc aaagtcaaaa
     6481 taagccttac ccgcagactt tagccaagga ataacagcat cggaatatga agtattgatt
     6541 gagattgcct cacagttggc cttatcgtat gcctccatta tattctttgc atatcgaata
     6601 gaaagcggag tccaaatgcc gtatgcacga agattactcc atgagatgct accatacttc
     6661 aaaccagagt aaactcgact actatttact atgaagtcag gcttttctgc ttcaagtaca
     6721 gctgcaatgc tgttcacatc gttaaggtcg cagttatcaa ccaccttaat ctcgctacgg
     6781 cactggtgac gaatggttga agcagtacga ataatattta tatccatctg catcttttcg
     6841 gcattacgtc ctaccacaac aagacgaagt tgtgaatcac ctagtgatac aagataatcc
     6901 aacaagtagc atccaacgct acccaaacca ataatcataa tagttattgg ctgactattt
     6961 tcaaccttcc ctttgagaag ggaaagtttt ctgttcagta tttccatttt aataaagtag
     7021 aaaacgggtt tgtaataaat ttagaatcaa caaatcgtaa tagaactgtt gtaaggatta
     7081 atatgcaaac acagagaaac aaggcctggt aaaaattaac ttgcaaatag gttgataata
     7141 tgtgtgcaaa catgccttgt attacataat atgctaaagt ctttgatcca taagcacata
     7201 tcgacttggg gaggttcaca aacattaaaa cagcgaatgt agtaataatt gatccaatat
     7261 aaaccaatac tttcaaaaca agaagcattc ctatattttg attgtacaaa tttgacatgt
     7321 gccacacatt ggcattatta aactctaata catgtagtat acggccacta tagatgagac
     7381 aaaaagcaaa acatactaaa gaggctatag ccaaaacaag ctttatgcat tttgaaacat
     7441 caggaataat aggcaggcct tttactcttc tcaagaaacc catcataaag aatggtagaa
     7501 acatacacga ccgttggaac gataacaggt taccaaattg tttcaacgga agcatgtatg
     7561 caaatacggc caacaagcaa ctgaatatca ataactgccc cccgcaccca ttatattgta
     7621 cgacaatttt gccaactagc ttattgataa tcaacagaaa aataatatac caagttatta
     7681 ggctgattaa ataccagcct gagttctccg gatttagtaa agggatgata cttccataat
     7741 aaaaacattg aggaatcatc acaaccaaaa atgtttccaa aagcataaaa cctttactaa
     7801 tcagtttttc tttatcctta acatttgcat aataaccact gataaagacg aataatggca
     7861 tatgaaaaat gtagattata gaatagagct tattggcaat aggattgctt aaagcaggtt
     7921 ctattacatg accgaatgta accaatacaa tgagcaacca ctttattcca tctattctgt
     7981 aatctctttc taatacagcc atattcattt ttacatcaat tttcttacaa tatcataatc
     8041 tgcaacagta ttgcagttga accagtcagt gaaagtcact acatcatact cttcactatt
     8101 catatcacta aagtaagcct gaattatttc aagattagta ccttgaagtt gttcttttgt
     8161 aagcgaatca acagtcttca aaagcctctc agatgaagaa aatttccaca tctgaccact
     8221 gttgaagatt gccttgaatg gctctgatac agtcaatgtc tgatggttcg tatcatataa
     8281 ataccttggt ttgccatcaa cgttaatgta taacgcaact gccttattcg agtagattgg
     8341 ttctcggtta atggtaatca cactctttgg cgaattatac acttgcctga aatctgtagc
     8401 cataaagaac aagtcgcctt ctacgaaagt cacatcacct gcctccttta ttgcctctat
     8461 acctttgtat aaactgtagc cggagccata gtctttgaaa tgctcgttgt aaacaagttc
     8521 tatttttttg ccataaccag agagttcgtt ctttacaaat cttcccaact tctcataaag
     8581 gtagcccccc acaaggatat atttatcgta ctcaccacaa ttcttcagca attgagcaag
     8641 aagagcgtat tgaggttcat cggtataata caagcatttg agggtttctt ccttagtgtc
     8701 gcgattaaaa cgcgtagccg ttccagcaac agttataatc aaatatttca tttcttgata
     8761 ctatttacac acatcgtgag gccttcatcg agagtgatga taggtttcca gctagtacgg
     8821 gtacgaatct tgtccgtatt gagcaatcga cgctcaggat cagactttcg gtaaccttcg
     8881 aaacggatat ccacatcgtt gatgcccatt tttattgcaa tcgtcttgac tagatcaata
     8941 atggcaattt cctcttcagt agccacatta taaacagtac catcaagagc atcctcagaa
     9001 gcgatgagct ccataacggc acgacagcta tcaatattat gaaggaaggt acgtttgttc
     9061 accttcgagt tttcaagaag ggttacggta tggctttcaa gcagattatc aatgatatga
     9121 gggataatgt gctctgcaaa acgctcgtgc ctgctatata cattagcaaa gcgaatagaa
     9181 caacctttaa ttcgtccctt gccaacagca tctttcataa agaattcagt catcaacttg
     9241 ccgcaagcat aacttgtacg ttggctatgt tccgctgttg acattgccaa ataatcagat
     9301 tctttcacac cgccattctc attccaagac tgcattgagt aaacctccga agttgaacaa
     9361 ttaatgtaag tatcagccct catagaaatg gcacgctcca agaaatcctt catagcaagg
     9421 acgttagtct gaaaagtact ctctacctca tagaaatgct ttgtatggac aacagcagca
     9481 caattgataa acaccagacg accatcgcac tcaattttga gttctttcgc cttatcgcat
     9541 acttcatcca tctgagcgac tttgttaatg tcgtaattat agaagatgaa cgagggattg
     9601 tcaataatgt ctttaacggt atcaatagaa cttgcaaaga agttgtcgaa accgattacc
     9661 gtatgaccct gagaaacaaa ctgacgagac agttcactac ccgtcatgcc agtaacacca
     9721 cttattacat ataagttctt catttatatt ccaaatttta atcgttcgct gtaaaaagta
     9781 tctatctctt tggggaaaag agattgttca gcttcaatat cgtaaggaac tgcattgaaa
     9841 tgggtttccc ccgtttcaaa attgatatac acatattgtg catatggatt ctgattgcga
     9901 ggttgtccaa ctgaacctgg gttgataaaa gtcgtacgtt tctgattgcg catgattgga
     9961 ttgtcaactt tgaagaatac atctatctta aaaggttgat gaatgtgacc gctcaatacg
    10021 tagtcaaaat cctgatactt ttcatcttcc acttttgctg gagtcagttt tccccaatag
    10081 ggatcatcaa tagttccatg taaaagaagt actttcttac caccaatttc caactcttgc
    10141 attggctctt gcatcacatt gcttatataa tcaatagatg tctgagtgag atggtttctt
    10201 gtgtatttgg acatcacacg agctctttct gaagagaaac atgagagttc cccatcaatc
    10261 aaagccttct cgtgattgcc cttcaaattg gcaagtaatg gaatattaac agtttgtata
    10321 cgttcaataa tttcatttga acgcatacca tagtcgataa agtcacctag actaatcatg
    10381 gcatctggct gatagtgttt ctcaatatca gctagaaccg cctcaaaagc agaaagattg
    10441 gcatgtatat cagcaaacag aattactttc atactttact tgttaagaaa ttccataaaa
    10501 gcctctttgt tctgaatagg agttgtagtt ggacggccaa ggtccttgcg gtttcccttt
    10561 tttacgcaaa tctgaaggaa aacaggacct tctgcgttct tgaattctgc aagttttgct
    10621 tcaagtgcct ccatagtttc tacactataa gcagccttgt agcctacagc ttgagcaatt
    10681 gcacaaaaat caatcttgag accaacagta ggctgaccac caacagaatc gtgtgcgcca
    10741 ttgttgaaca caatgtgcac ataattcttg catttcatgc tggctgtaat agccatatta
    10801 cccatgtgca tgatgctggc tccatcacca tcaaaacaat atactcgacg ctcaggcttt
    10861 tggagagcta tgccgagagc tatctgggaa gcatggccca tcgcccctac agtaaggaaa
    10921 tcacgttcat gaccttggct ccaagcagta cgtgcctcaa agagctcgcg agaaatcata
    10981 ccagtagttg atacgataca agcattatct tcaatgtttg aagccacctt ctgaatcgtc
    11041 tcttcgcgag acattgccag ttcgtttacc tcaacgctct gaagcttata ggtatcgaaa
    11101 gtatccttct ctatcacaag ggcatagcac tcgttggttg ccttcatgaa gtcgacagct
    11161 atttttatct gctttgcagc tccctcttca tcctttggca gaatagcata gttgataccc
    11221 atggtattaa gcaaaccgat tgtcacctta ccttgcttca catgctgagg ttcatcgtga
    11281 actccagggc gccctctcca accgataacg aggagcacag gaatgttata aacgtccttg
    11341 tcggtaagag aggcaaacgg attaatgata ttgccttcac cagagttctg catataaact
    11401 acaggcacct tacctgttgc caaatgataa cctacagcaa tacccatggc ccctccttcg
    11461 ttagcagcaa taatattctg ctcagcagga aggtggtcgg taatatatgc acaaatgttc
    11521 ttgagcaacg aatcaggaac accagcataa aagtctatac cttgttcctt gagtatattt
    11581 acaaaaaatt cgggtcttac catttgtaaa atagtttaag gggtaagaaa ttaatgttta
    11641 cttcttcttt gtgcctggaa taagattaag aatctgtttg ataggcatac agtactgctc
    11701 agatgcttca agcgaacggc tagtatagag gatggtttct gcacatttaa ccattgcagg
    11761 ataagcagaa cgaagcatgt gattagcata aatcactaca ttaatacccc attcagcaag
    11821 ttcctcttct gtgaactggt tataagtagt aggcacaacg atgataggag tatgagcatt
    11881 cttctcgcga aagcgcaagc aaaactcctt gatgtcctct ccagacttct ctttggagtg
    11941 gatcattata ccgtcagtac ctgcctctac gtaagcatga caacgctcca aagcatcatc
    12001 tacagtctta ccagcaatga gagactcaca acggctgaca atcatgaagt cacgagtaat
    12061 ttgcgcttcc ttacctgcac gaattttagc ctgaaaacct tcgattgtat cctgagtctg
    12121 tacagcatca gtaccaaaaa gtgaattctg ttttaaacct accttatctt caattataac
    12181 gcaagagatt cccaagcgct caagggtgcg aacggtaaaa ccgaagtgtt caatcttgcc
    12241 accagtatcg ccatcgaaga taacaggttt tgtggttact tcaagggtat cattgaggtc
    12301 atggagacgg gtagtcaaat cgacagcctc aatatcaggc ttacccttag aggtagagtc
    12361 ggtaagggag gaagcccaca tgccatcgaa ttcacgctcc tgaccattca cctctacctt
    12421 agtatgttct gctatgaggc cagtaagacc attgtgactt tcaagaatac gtacaatcgg
    12481 tttagctgca ataaggcgac ggagagaaga aagacgcatc tgaggagtta caccaagagc
    12541 atcaatctct tgtttgatac cagaagcaga gatgttttgg gtgtaaggaa tctcgataac
    12601 ctcaccaccc aacttttcca ttactttgaa tacctcatca cggatatact tgtcaggacc
    12661 cacctgccaa tcatcaccat gaatgatgta atcaggcttg tacttaagga gattagagac
    12721 atagctccaa tcatcctgag ggaccaccct tgatacgttc ctgatgctct caacaacgtt
    12781 ctttctttgc tcccaagtga gataaggaag tctacggtgg ttagcaatcg ccttatcagt
    12841 gaacaaaccg attatcacat cgccatactc agtagctttg ttaatgatgt taatgtaacc
    12901 cggatgcatg atatcaccta tcataccaag ataaactgtc tttgccatta ttatttaaca
    12961 tttttcaaat tattataaag agcatctata gtcaatcacc tcaatccctc ctgcttgaga
    13021 ataagtgtgc aacaaaatcg gctttctacc atcactcagg actatactct gaccagtgag
    13081 ttctgctacc tttttatctt taataaaatc aaaattctta cttgtcttat acacaaagat
    13141 cgactctcca tatgcacgac tgcatttttg tgccactcga taaatttctc catctacaga
    13201 gaataacgaa cctgcgggac gattaatctt ctttttgtcg cgatactcgt caaccctaat
    13261 tatcttcatc gtattcatgt caagaataaa cttctcaaca taatacttaa ctgtaaagct
    13321 atttgttcca acctgacgat aagtcaaaag agtggaagat tattcttaac aagcttatca
    13381 cgaaaatact tagccaattc tgcacaacgc gcaatctcca cctccgcacc acctgcagac
    13441 tcaatttctt ttaaacggac attaatttga cgaaggatac caacagcagg agtacaagga
    13501 gtctgccctc tctccatatt tttaagagca agcttcaaat caagatactg acagcaacat
    13561 tggacctttt ctatacgttt aatagccgaa ggagcaagaa gcataactgc aatacccgga
    13621 gcgcaagcaa gagccttctg acttccagta atcatcaccc ctacatccat tgccaccata
    13681 tcaaatgaat cgcaaaggaa ggtagagatt atatcaacaa tcaagaaaca attattacgt
    13741 ttgcaaaaat cagaaacaag attgatatcg taatgtaccc ccgtaaaagt ttcgtgcttc
    13801 tgcataagga aagtagtgta acccttgctc tcatattcag caagatgttc aggcttcaaa
    13861 gctctaccat acttaagctt aatttccgta aaaggaattt tatgaagggt aagcaattcc
    13921 acaaaacgct caccaaagct accaccattg attacaagag ccttatcctg tggcgtaagg
    13981 caatttatga tggcagcctc cataccacca gagccagaac atgtcatgaa aacaacctta
    14041 ctatctttag tcgcattagc aaatttctta acaaggcgct cattctcaag catcatttct
    14101 gaaaactctg ctgttctaaa tatggtacct gttcggcacc tatgacccgt acagcatcag
    14161 aagattgcac cggacctact gtaaaattaa tcatttatat agattccttt aattatcagt
    14221 aataatcaac caatccaatc accactcccc cttcttccta acctccagcc atttttccac
    14281 ccattcccca gccaatttcc tgatttcgtc atcatccagt tcctccagtt catcgatata
    14341 gtatcgcaga agagtttcgt atgtactaag tgaggcgtta cacccttttt tttaagtcta
    14401 tgtacgtgga tggagaaata tgcaaatcct tgcaaagagt ttcaatagtg actccactct
    14461 gttttctcgt tcgctcgata aaaaagccca atgaagggtg aataaaattt ttgcccattt
    14521 agataaattt taagtaatta gatagaatac tatgttatat at
//
