LOCUS       c00596_gnlPro..         4025 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DEAPBPJF_596.
ACCESSION   c00596_gnlPro..
VERSION     c00596_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 12:10:24
            Original ID  :: gnl|Prokka|DEAPBPJF_596
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4025
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4025
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3964]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3964
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4025
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4025
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..596)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg596_1"
                     /sec_met_domain="OAD_beta (E-value: 5.2e-72, bitscore:
                     231.1, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESFLTFLGENLQLFLTYTGVYNATPGHLIMILVGLLFIFLAIK
                     YEFEPMLLIPIGFGILIGNIPFKDAGLQVGIYEQGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYTIALQMGFEPNQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPIIQPPFMRLLT"
     CDS             complement(601..1032)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 64.8; E-value:
                     1.6e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg596_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.3e-21, bitscore:
                     64.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSFKYTINGNVYKVHINSVVDDIAELEVNGTPYQVKMEKPAKKQ
                     MVTLKRPAQAPTTASGDPVVSRPAASTTQGAVKTPLPGVILQVKCNVGDTVKRGQTLII
                     LEAMKMENNINADRDGKIIEIKVHKGDSVLEGADLVVIG"
     CDS             complement(1055..1942)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg596_3"
                     /sec_met_domain="OAD_gamma (E-value: 4e-13, bitscore: 38.4,
                     seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINKKIGVLLLFALLISFGAKAQRATSMRINEVLVINEDNFVDDY
                     GKRHGWIELFNTSAGTVNIAGCYLTDDKNNPMKYPIPKGDVLTQIQPRQHTLFWADGQP
                     GRGTFHVNFVLDPSKENYVALYDADGKTLIDEITIPAGQLADISYGRVIDGEKDWAQLK
                     KVTPSTNNLTLDSNEKIDNFRVNDSLGIGMTITAMAVVFLGLFLLYIIFKQIGKLSIAA
                     SKRNVEKAVGSASVTADAGQESGEIFAAIATALYEMSDDNHDIENTVLTIRKVQRAYSP
                     WSSKLYGLRQTPQK"
     CDS             complement(1964..3517)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg596_4"
                     /sec_met_domain="CT_Btheta (E-value: 4.2e-293, bitscore:
                     960.5, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALREQARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIFAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVVPQI
                     SGIFGPCAGGAVYSPALTDFNIMTRGTSYMFLTGPKVVKTVTGEDVTQEQLGGASVHTT
                     KSGVAHFAVDTEEDGLQLIRKLLSFLPQNNLEETPLVECKDPIDRLDDVLNDIIPDNAN
                     QSYDMYEVIGTIVDNNEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKVMAGCLDSNA
                     SRKAGRFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKITV
                     TLRKSYGGAYCVMSSKHLRGDMNYAWPTAEIAVMGPSGAVEVIFAKEVAAAEDPKACAA
                     EKEAEYKKAFANPYNAAQYGYIDDVIEPRNTRFRIIRALQQLQTKKLSNPAKKHDNLPL
                     "
     CDS             complement(3554..3964)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg596_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.1e-27, bitscore:
                     85.0, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEGVLGLKCYNIEVVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALNEAEEKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGNK"
ORIGIN
        1 gtcgtaagca atctcatgaa cggtggttgg ataataggaa ccaacgccat gtatgagtag
       61 gcggataccg caatcgcacc cattaagttt ggcgctaact tagaagaaag gaagatagcc
      121 gtcggaccgt cagcaccacc gataataccg atagcacctg cttggttcgg ctcaaatccc
      181 atttgaagag caatcgtata agcaccaaag ataccgaact gagccgctgc accgatcaac
      241 atcaactttg gattggagat caatgcggag aagtcggtca tggccccgat gccgaggaag
      301 atcaatggcg gataccatcc agaggtcaca ccctgataga ggatgttcag tacagaccct
      361 tgctcataaa tacctacttg aagacctgcg tccttgaaag ggatattacc gatcaagata
      421 ccgaatccga tagggatcaa aagcatcggt tcaaactcat atttaatcgc caggaatata
      481 aacaacagac ctaccagaat catgatgaga tgtcccggag tagcgttgta tacacctgta
      541 taggtaagga acagttgtaa gttctcacct aagaatgtta aaaagctctc ttgcatagtc
      601 ttatccgatt actacgagat cagcaccttc tagcacagag tctcccttgt gtactttaat
      661 ttctataatc ttaccgtcac ggtctgcgtt gatgttgttc tccatcttca tagcctcaag
      721 gataatcaag gtttgacctc tcttcacggt atcacctacg ttgcacttaa cctgcaagat
      781 aacacctggt agcggagtct taacagcccc ttgtgtcgta gaagccgccg gacgggaaac
      841 aacagggtct ccacttgccg tagtcggggc ttgagctggg cgtttcaatg taaccatttg
      901 tttctttgcc ggtttctcca tcttaacttg gtagggagtt ccgtttactt caagttctgc
      961 gatatcatcc actacagaat tgatgtgtac tttatatacg ttaccgttaa tggtatattt
     1021 aaaacttttc attctttagt tcgtttttag ataattactt ttgaggagtt tgacgtaagc
     1081 catagagttt tgaactccaa ggagaataag ctcgttgtac cttacgtata gtgagaaccg
     1141 tgttctcgat atcgtgatta tcatcgctca tttcgtatag agcggtagcg atggcggcga
     1201 atatctctcc agattcttgt cctgcgtctg cggtaactga agcagaacct acggccttct
     1261 ctacgttacg tttgctagcg gcgatagata atttaccaat ctgcttaaag atgatgtaca
     1321 acaagaacag acctaagaat acaacagcca tagccgtgat agtcataccg atacctaaag
     1381 agtcattaac cctgaagtta tcgattttct cattggagtc caacgtcaag ttgttcgtac
     1441 tgggagtgac tttctttaac tgtgcccagt ctttttcgcc atcaatcact ctaccgtagc
     1501 taatgtctgc taattggcct gccggaattg tgatctcatc gatcaaggtc ttaccgtcgg
     1561 cgtcatataa ggcaacataa ttctccttag agggatctaa tacgaagtta acgtggaatg
     1621 tacctctgcc cggctgtccg tcagcccaaa atagtgtgtg ctggcggggt tggatctgag
     1681 ttaatacgtc tcccttcggg atcggatatt tcatgggatt gtttttgtcg tctgtcaaat
     1741 agcatcccgc gatatttacc gttccggccg aagtgttgaa caactcaatc cagccatgac
     1801 gtttgccata gtcatcaacg aagttatcct cgttgataac caatacttca ttaattcgca
     1861 tggaggttgc gcgctgtgcc tttgccccga atgaaatgag caaagcgaac aatagcaata
     1921 cccctatttt cttatttatc atattcatct cctgttttac ggattataga ggcaggttat
     1981 catgtttctt agcaggattg cttagcttct tagtctgcaa ttgctgcaaa gcacgaatta
     2041 tacggaaacg agtgttacgc ggttcgataa catcgtcgat ataaccgtat tgagctgcgt
     2101 tataaggatt agcgaatgct ttcttatatt cagcttcttt ctctgctgcg catgctttcg
     2161 gatcttcagc ggctgctact tccttggcga agataacctc tacggcaccg ctcgggccca
     2221 taacggcgat ctcggctgtc ggccatgcgt aattcatatc accacgtaaa tgtttagaac
     2281 tcatcacgca ataagctcca ccgtaagact tacgcaatgt aacggtaatc ttaggaacgg
     2341 tagcctcacc ataagcgtaa agcaatttag cgccatgaag gattacaccg ttgtattcct
     2401 gacctgtacc cggaaggaat cccggaacgt caaccaatgt taccaaagga atattgaaag
     2461 cgtcgcagaa acgaacgaag cgacctgctt tacgagatgc gttgctatcc aagcatcctg
     2521 ccataacctt cggctggttg gctacgatac cgacagattg tccgttgaaa cgagcgaaac
     2581 ctacgatgat attcttagcg tagtccgcat ggacctctag gaactcgttg ttatcgacga
     2641 tcgtaccgat aacctcatac atatcgtaag attggttagc gttatcggga atgatatcgt
     2701 tcaatacatc atccagacga tcgatcgggt ctttacactc aactaacggc gtttcctcta
     2761 agttgttctg aggtaagaaa ctgagcaatt tacgaatcaa ttgcaaacca tcctcttctg
     2821 tatcaacagc gaaatgggct acacctgatt ttgtagtgtg tacgctggct ccacccaact
     2881 gctcttgggt tacatcctca cctgttacgg tttttactac tttcggacct gtcaagaaca
     2941 tgtagctggt accacgtgtc atgatgttga agtctgtcaa agccggagaa taaacggctc
     3001 caccggcgca agggccgaag ataccggaaa tctggggaac aacaccagat gccaagatgt
     3061 tacgttggaa aatctccgca tatcctgcca aggcgtttac accttcttgg atacgagctc
     3121 cgccggagtc gttcaaaccg ataacgggag cgcccatctt catggcttga tccattactt
     3181 tacagatctt catagccaaa gcctcagaca aggctccacc gaatacggtg aaatcttgag
     3241 cgaagatata taccaaacgt ccgtcgatcg taccataacc tgttacgata ccatcgccta
     3301 aatatttagt tttctcgata ccgaagtttg tacaacggtg ttgaacaaac atgtcgaatt
     3361 cctcgaaact accttcgtca agcaacatgg cgatacgctc acgagcggta tatttgcctt
     3421 ttttgtgctg tgattcgatt cttttctctc ctccacccaa gcgagcttgc tcacgaagtg
     3481 cgatgagctc ttttactttt tcaagctggt tactcattgt attttgattt gttgttagaa
     3541 atgaacggat taattatttg ttgcccggca tgcaaagttc tgtcaatacg ctgcaagttg
     3601 atttcgggtg aaggaaagcg atgttcaagc cttccgcgcc accacgagga gctttatcga
     3661 ttaatttaac gcctttttcc tcagcctcgt tcaaagctgc ctgtacatca ggtaccgcga
     3721 atgcgatatg gtggatacct tcaccttttt tctcgataaa tttcgcgatc gtgctatctt
     3781 cgctggtcgg ctctagtagt tcaatcttag tctgtcctac cttgaaaaat gcggttttta
     3841 ctttttggtc tgctactact tcgatattgt agcattttaa tccaagaacg ccttcataat
     3901 agggtaaaca agcctcgata ctcttcactg cgatacctaa atgttcgata tgtgtaagtt
     3961 ccatgtcaat aataatttaa agatttagta aatgtttgtt tctattgtgt gcaaatgtac
     4021 actac
//
