LOCUS       c00307_gnlPro..         4346 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PNPANNIN_307.
ACCESSION   c00307_gnlPro..
VERSION     c00307_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:46:14
            Original ID  :: gnl|Prokka|PNPANNIN_307
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4346
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4346
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[161:4346]"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      162..4346
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /neighbourhood="10000"
                     /product="acetate2butyrate"
                     /protocluster_number="1"
     cand_cluster    1..4346
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(not (POR or POR_N or PFOR_II) and
                     minimum(4, [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF,
                     ETF_alpha, HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and
                     minimum(1, [EC1_2-faecali, ECH1_Enoyl_CoA_DH1,
                     ECH1_Enoyl_CoA_DH2, ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4,
                     Thiolase_C]))"
                     /kind="single"
                     /product="acetate2butyrate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4346
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="acetate2butyrate"
                     /region_number="1"
                     /rules="(not (POR or POR_N or PFOR_II) and minimum(4,
                     [Acyl-CoA_dh_1, Acyl-CoA_dh_2, ECH_2, ETF, ETF_alpha,
                     HCDH_N, Thiolase_N, ketoacyl-synt]) and HCDH and minimum(1,
                     [EC1_2-faecali, ECH1_Enoyl_CoA_DH1, ECH1_Enoyl_CoA_DH2,
                     ECH1_Enoyl_CoA_DH3, ECH1_Enoyl_CoA_DH4, Thiolase_C]))"
                     /tool="gutsmash"
     CDS             complement(162..1208)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF_alpha"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 161.3;
                     E-value: 7.7e-50)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg307_1"
                     /sec_met_domain="ETF (E-value: 6.2e-51, bitscore: 161.3,
                     seeds: 129, tool: rule-based-clusters)"
                     /sec_met_domain="ETF_alpha (E-value: 2.7e-39, bitscore:
                     121.7, seeds: 511, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALEEYKGVFVFAQQVDNVLDGVAFELLGKGKDLAKDLGTDVTAV
                     LIGSGVKGLADQLAEYGADKVIVVDDPELKDYRTEPYAHALASVINEFKPEIMLVGATA
                     IGRDLGPTVSARVKTGLTADCTSLEIGDFPLVAAPGKESEQKHNQLLMTRPAFGGNTIA
                     TIACPDNRPQMATVRPGVMQKIAPISGAKANVVEYNPGFTPNNRYVEILNIVKAVKSTA
                     NIMEAKILVSGGRGVGSKENFKLLEDLAEVLGGTVSCSRAVVENGWLPVDLQVGQTGKT
                     VRPQIYFAIGISGAIQHVAGMEDSDLIIAINKDEDAPIFDVADYGLVGDLNKIVPALTT
                     ALKAELGK"
     CDS             complement(1235..2017)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: ETF"
                     /gene_functions="electron_transport (smcogs)
                     SMCOG1008:Electron-transferring flavoprotein (Score: 159.1;
                     E-value: 3.7e-49)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg307_2"
                     /sec_met_domain="ETF (E-value: 3e-50, bitscore: 159.1,
                     seeds: 129, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIVVCIKQVPDTKGGVKFNPDGTLDRAAMLAIMNPDDKAGLEAA
                     LRIKDETGAKVTVLTMGLPKADAVLREALAMGADEAVLVTDRVLGGADTWATSTTIAGA
                     LRNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLPVISYAADIKVEGDSVVVKRQYEDRY
                     HELKAKMPCLVTALSELNEPRYMTPGGIFDAFDKEVTVWGRADLKDVDDSDLGLKGSPT
                     KIAKASDKVAKGAGEKVNLDPAESVAYLIGKFKEKHII"
     CDS             complement(2074..3228)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg307_3"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.8e-53, bitscore:
                     168.6, seeds: 49, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1e-31, bitscore:
                     97.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.9e-40, bitscore:
                     126.6, seeds: 796, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFTLDKKHEMARSLFKEFAETEVKPLAQETDETEAFPAETVKKM
                     QKYGFMGIPVPKEYGGQGCDPLTYVMCVEELSKVCATTGVVVSAHTSLCIDPIMTYGTE
                     EQKQKYVKPLATGEKLGAFALTEPGAGTDAQGAQTKAVLDGDEWVLNGSKCFITNGKVA
                     DVYIVIAITSITEDKRGRKKKNFSAFIVDKGAPGFSFGTKEKKMGIRGSSTYELIFEDC
                     RIPKENLLGPEGKGFPIAMHTLDGGRIGIAAQALGIAEGALDRAIAYTKERKQFGRSIA
                     QQQNTQFKLADMAARIEAAQLLVYKAAMAKATQKVYSVEAAKAKLFAAETAMAVTTEVV
                     QLFGGYGYIREYDVERMMRDAKITEIYEGTSEVQRMVISGALLK"
     CDS             complement(3258..4130)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: HCDH_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg307_4"
                     /sec_met_domain="HCDH (E-value: 8.4e-36, bitscore: 111.1,
                     seeds: 68, tool: rule-based-clusters)"
                     /sec_met_domain="HCDH_N (E-value: 4e-64, bitscore: 204.2,
                     seeds: 60, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGVIGAGTMGQGIAKAFAQVEGYEVALCDIKQEWAEGGKEKIK
                     KGYARLVEKGKMTQEAVDGILAKITPGLKEDLCKDCDLIVEAAFENMEVKKTTFKELDE
                     IAKPDCIFASNTSSLSITEIGNGLNRPMVGMHFFNPADRMKLIEVIAGVNTPDETVAAI
                     KKISEEIGKTPVQVNEAAGFVVNRILIPMINEAAFIKMEGVSDIAGIDAAMKLGANHPM
                     GPLELGDFIGLDICLAIMDVLYKETGDSKYRACPLIRKMVRGGNLGAKSGKGFYVYNAD
                     RTKTPVDAQ"
     CDS             complement(4224..4346)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     acetate2butyrate: Thiolase_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg307_5"
                     /sec_met_domain="Thiolase_C (E-value: 1.8e-16, bitscore:
                     48.4, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="ASGCRILVTLLHEMQKRDAKRGLATLCIGGGMGCSTIVER"
ORIGIN
        1 ctcaactcct gaccaagagc ctcaaatttc ctccaactcc aaacaagaag cctcaaattt
       61 tctccagctc ccagcaaaaa gcccgtcctt cctgtcagct tcctcacaga agccgcacac
      121 gaaagacggg ctttctatct tacaggttat tcacctatgt attatttgcc taactcagct
      181 tttaatgctg ttgttaaagc aggaacgatc ttattcagat caccaaccag accgtaatca
      241 gctacgtcga agattggagc atcttcatct ttgttgattg cgatgatcag gtcagaatct
      301 tccataccag ctacgtgctg gattgctccg gaaataccga ttgcaaagta gatctgagga
      361 cgaacagttt taccagtctg acctacctgt aaatcaactg gtaaccagcc attctctaca
      421 actgcacggg agcagcttac agtaccgcca agtacctctg caagatcttc caaaagtttg
      481 aagttctctt tggaaccaac tccacgacca ccggaaacaa ggatctttgc ttccataatg
      541 tttgctgtgc tcttaacagc ttttacgatg tttaagatct caacatatct gttgtttggt
      601 gtaaatcccg ggttgtactc tacaacgttt gcttttgcac cgctgatcgg agcgatcttc
      661 tgcataacac ctggacgtac agttgccatc tgaggacggt tgtccgggca ggcaattgtt
      721 gcgatggtgt taccaccaaa tgcaggacgt gtcattaata actggttgtg tttctgctct
      781 gattctttac caggtgcagc aaccagtggg aaatcaccga tctcaagaga tgtacagtct
      841 gctgtcagac ctgtttttac tcttgcagaa acagtaggtc ctaagtcacg gccgatagca
      901 gttgcaccaa ctaacatgat ttcaggtttg aactcgttaa ttacagatgc taatgcatga
      961 gcatacggct ctgttctgta gtcttttaat tcaggatcat ctactacgat aactttgtca
     1021 gcaccatact cagctaactg atctgccaat ccttttacac cagaaccaat taatacagca
     1081 gttacgtctg tacctaaatc ttttgctaag tctttgcctt ttccaagtaa ctcaaatgca
     1141 acgccatcta atacgttatc tacctgctgt gcaaagacaa atactccttt atattcttct
     1201 aatgccatgt tattcaccac ttttcctttt tttattagat aatgtgtttc tctttgaatt
     1261 taccgatcaa atatgctacg gattctgccg gatcaaggtt tactttctca ccagcaccct
     1321 ttgcaacttt atcagatgct tttgcaatct tagtaggaga tccctttaaa ccaagatcag
     1381 aatcgtcaac atcctttaag tctgctctgc cccaaacggt tacttctttg tcaaatgcat
     1441 cgaagattcc gcccggtgtc atgtaacgcg gctcatttaa ttcagaaaga gcagttacca
     1501 gacaaggcat ttttgctttt aactcatgat atctgtcttc gtactgacgt tttacaacaa
     1561 cagaatcacc ctctactttg atgtctgctg cgtaagagat aaccggaagt ccaagatgct
     1621 cagcgatctg aggaccaacc tgtgcagtat caccatcgat agcctgacgt cctgtgatga
     1681 tcaggtcata gtctaagtta cgaagtgcac cagcgattgt ggtagatgtt gcccatgtat
     1741 cagctccacc taatacgcgg tctgttacaa gaactgcttc atcagcaccc attgctaatg
     1801 cttcacgaag aactgcatca gcctttggaa gtcccatggt aagaactgta acttttgctc
     1861 ctgtctcatc tttgattcta agtgctgctt caagaccagc tttatcatct gggttcatga
     1921 tagcaagcat tgctgctctg tcaagcgttc catccgggtt gaacttaacg ccgccctttg
     1981 tatcaggaac ctgtttaata catactacga tattcatgta tttttcactc cttatgtttt
     2041 atatgtttaa attttacgcg gattgattcg aaattacttt aacagtgcac cggagattac
     2101 catacgctgg acttcgcttg taccctcgta gatctcagtg atcttagcat cacgcatcat
     2161 acgctctaca tcgtactctc tgatatatcc gtatccaccg aataactgaa caacctcagt
     2221 tgtaacagcc attgcagtct ctgctgcgaa taattttgct tttgcagctt ctacagaata
     2281 tactttctga gtagcttttg ccattgctgc tttgtaaact aaaagctgtg cagcttcgat
     2341 tcttgcagcc atatcagcaa gtttgaactg tgtattctgc tgctgtgcga ttgaacgtcc
     2401 aaactgtttt ctctcttttg tataagcgat agctctgtct aatgcgccct cagcgatacc
     2461 aagtgcctga gcagcaatac cgatacgtcc accatcaagt gtgtgcattg cgatcgggaa
     2521 accttttcct tccggtccta acagattctc tttcgggatt ctgcagtctt cgaagatcag
     2581 ctcataagta gatgatccac ggatacccat cttcttctct tttgttccga atgagaagcc
     2641 tggagctccc ttatcaacga tgaatgcaga gaagtttttc ttctttctgc ctctcttatc
     2701 ctctgtgatg cttgtgatag cgattacaat ataaacatct gcaactttac cattggtaat
     2761 gaagcatttt gatccattta atacccactc gtcaccatct aaaacagctt ttgtctgtgc
     2821 accctgtgca tcagtaccag cccccggctc agttaatgcg aatgcaccta acttctcgcc
     2881 tgttgcaagt ggttttacat atttctgttt ctgctcttct gtaccatatg tcatgatcgg
     2941 atcgatgcag agagatgtat gtgcagatac aacaacacct gtggttgcgc aaactttaga
     3001 taactcttct acgcacataa cgtatgtaag aggatcacaa ccctgtccac catattcttt
     3061 cggaaccggg attcccatga atccgtattt ctgcattttc ttaaccgttt ctgccgggaa
     3121 agcttctgtc tcatctgttt cctgtgcaag cggttttact tctgtttcag caaattcctt
     3181 gaataaggaa cgcgccattt catgtttctt gtctaaagtg aaatccatga tctaattttc
     3241 ctccataaat atttatttta ctgagcatcg accggagtct ttgtacgatc agcattgtat
     3301 acatagaatc ccttgccgct ctttgctcca aggttaccac cacgaaccat cttacggatc
     3361 agtgggcatg cacgatattt gctgtcgcct gtctctttgt aaagtacatc catgatagca
     3421 aggcagatat caagaccgat gaaatcacct aactctaacg gtcccattgg gtggttagca
     3481 ccaagtttca tagctgcatc gataccagcg atatcggaaa caccttccat tttgatgaat
     3541 gcagcttcgt tgatcattgg gattaagata cggtttacaa cgaaaccagc agcctcatta
     3601 acctgtaccg gagtttttcc gatctcttca gaaattttct taatagctgc aacagtttca
     3661 tccggagtat tcacaccagc gataacctcg atcagtttca tacggtctgc cgggttgaag
     3721 aaatgcatac caaccattgg acggtttaat ccgtttccga tctctgtgat agaaagactg
     3781 gatgtgttag aagcgaagat acaatctggt tttgcgatct cgtctaattc tttgaaagta
     3841 gttttcttta cttccatgtt ctcgaaagct gcctctacga tcagatcaca gtctttgcaa
     3901 aggtcttctt ttaaacctgg agtgattttt gcaaggatgc catcaacagc ttcctgtgtc
     3961 atttttcctt tttctactaa tcttgcataa ccttttttga ttttctcttt tccgccttca
     4021 gcccactcct gcttgatatc gcaaagtgct acttcatatc cttctacctg agcaaatgct
     4081 tttgcaatgc cctgtcccat agttcctgca ccaataacgc caaccttcat ttcggttacc
     4141 tcctgaaaat aattatgtta actgtatgtt tgaaaaatgc aatccccggg aagtcattcc
     4201 ccgggaatta ctataaaaaa ctattatctc tcaacgattg tagagcatcc cataccaccg
     4261 ccgatgcaaa gtgtagcaag acctctctta gcgtctctct tctgcatctc atgcagtaat
     4321 gtaacaagga tacggcatcc ggaagc
//
