LOCUS       c00070_gnlPro..         7671 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GKKKACFH_70.
ACCESSION   c00070_gnlPro..
VERSION     c00070_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:43:56
            Original ID  :: gnl|Prokka|GKKKACFH_70
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7671
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7671
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[4668:7639]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      4669..7639
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7671
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7671
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..179)
                     /locus_tag="ctg70_1"
                     /transl_table=11
                     /translation="MLFNYTTHFLVLYYFSSSSPPKVRGVREELKLFLFYFFISTFYIS
                     LKKKIRMLEILSHS"
     CDS             196..528
                     /locus_tag="ctg70_2"
                     /transl_table=11
                     /translation="MKREIIAYKGYFKEFFENLDAGTQDKILYVLMLLQTQDRIPLKFM
                     RLIEEGLYELRIEYQSNIYRIFFCFDEGRIVILFNGFQKKTEKTPKKEIDKAKILRKEY
                     YGSKNK"
     CDS             509..814
                     /locus_tag="ctg70_3"
                     /transl_table=11
                     /translation="MEAKTNEEFFNVSALIDDRFGKEGTASRAEAEEKAYAFYTGQIIE
                     DARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTFYRIMNALGCKIEFSMIL"
     CDS             complement(884..2998)
                     /locus_tag="ctg70_4"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIVGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYACTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGRELN
                     AFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHPYSIQF
                     ITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEMVLTHIRKPER
                     FEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNGRRYAVFIIDI
                     EQVKSNRLYE"
     CDS             complement(3085..3315)
                     /locus_tag="ctg70_5"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3509..4012
                     /locus_tag="ctg70_6"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVLTRAVAQAGAGDDGGEDPDKTPGSGGDGSGDEEAP
                     DPTV"
     CDS             4073..4240
                     /locus_tag="ctg70_7"
                     /transl_table=11
                     /translation="MKEIVNRILDVIMYLIPFFGKRKRDKVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             4227..4676
                     /locus_tag="ctg70_8"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDEDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(4669..5397)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.6;
                     E-value: 1.3e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg70_9"
                     /sec_met_domain="Radical_SAM (E-value: 1.7e-28, bitscore:
                     89.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFCCLYCANPDTIAGKG
                     GTPTSPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(5411..7639)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg70_10"
                     /sec_met_domain="Gly_radical (E-value: 6.2e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1174.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDERVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 gaggagtgag agaggatttc taacattcgt attttttttt ttagagaaat atagaaagta
       61 gaaataaaga aataaaatag aaaaagtttc aattcctctc tcactcctct cacttttggg
      121 ggtgaagatg atgaaaaata atataaaacg aggaaatgtg ttgtatagtt gaacaacatt
      181 ccttatattt gcgttatgaa aagagaaata atagcatata aaggatactt caaagaattc
      241 tttgaaaatt tagatgctgg cacccaagat aagatattgt atgtgctaat gttattgcag
      301 acacaagaca gaataccttt gaaatttatg cggttgatag aagaaggact ttatgaactt
      361 cgtatcgagt accaaagcaa tatatataga atattctttt gtttcgacga agggcgtatt
      421 gtgattctgt ttaacggttt tcaaaagaaa accgaaaaaa caccaaagaa agaaattgat
      481 aaagcaaaga tattaagaaa ggagtattat ggaagcaaaa acaaatgagg aattctttaa
      541 tgtcagtgca ttaatagatg atcgttttgg taaggaagga acagcttctc gtgcagaggc
      601 agaagaaaag gcctacgcct tttatacagg acagattatc gaggacgcaa gaaaaaaggc
      661 taaaatcact caggctgaat tagcccggcg tatcggctct gaccgttctt atatttcaag
      721 agtagaaagt ggtcagacag agcctaaagt atctactttc tatcgtataa tgaatgcgtt
      781 aggctgtaaa atcgaatttt caatgatttt gtaaaaacaa taaatgcaga gtatctgtca
      841 agatgactct gcattttgct atattcacaa ggctttcccg cttctattca tacagcctgt
      901 ttgattttac ctgctcgata tctataataa atacggcata tcggcgcccg ttatttcctt
      961 ttttagattc aaaatggtgc ttagtcatca ccttccccaa ctgtattttt gatccgatgg
     1021 aatactgata accggtacgc tcacgtatta gttccgcaat ctcactgaca gtcagataat
     1081 aaaccttctc gaaacgttcc ggcttgcgga tatgcgtaag aaccatttct tcttccggtg
     1141 aatgaaagat gaaagcctcg ttattctctt caagccgttc attgtccgct tgcgtgaacc
     1201 agtattgata tcccggctta ttcagcagat atttgatctg agcatagagc tgcgcataat
     1261 tgatggtaat aaattggatg gaataaggat gaaaacaaag gaaacgccgg ttgcctgtcg
     1321 tgtcgtgaag cacttcctga taattgcagg ttcctgcaaa agaggccgta tgagggaagt
     1381 tttgcgaacg gcgtgcataa ggcagacgga aactgatcac tttgcgcgtt accaaatctt
     1441 tgaatgcatt cagttcccga ccgctcatcc cttcaaactc gtcgagattg atcagcatgg
     1501 cctgcgttat tttggctaaa tcatctttat tgttcgaatt gattagtccg gtagagagat
     1561 aagcacgcag ttcgggaggc aggagattat tgataaaggt agtctttcct atgttctgta
     1621 tctcgctgca tagcagaagt accgtgtgat tgacaatatt ctcttgagta gccgctgcgc
     1681 acatcgccac cagataacgt tccaggcatt ccgcccaaaa ctcctgatga ctcgtctgga
     1741 cactatccgc caggatacgg atatagtcgg ttccgtccca ttcgggcagt aattcaaaat
     1801 actcgcgaat gggatgatac gactggctga aatcggagta aatcagattt tcgattgttt
     1861 tgacagtgca ggcatatccc agttcgttga tttctatcca gatactgttt tccatcatct
     1921 catccaagat gcggaaaggc tccggtttct cggtgtcggg atgccttctg cgatattcca
     1981 tgatatgcag cacttcgttg tagcgtgttt cgtagtgctc gctgatatat tgctctatcc
     2041 gtaatattcg tttctgcgtg ccatttagct tacaggtatt aaattcgtcg gtatgagcgt
     2101 aggcgctatt gatcaaagag gctgtctcgt ccgccggaaa gtctgtaaac tggcttttga
     2161 taaaggcgga tgcttcctcc tgcggaatgc cgtagcgatt gaaggtacat gagagatggt
     2221 gcagataatt gttccggttg cccactatgt atttttcgga cttgttgtga tagtaggtta
     2281 aagtcagcac cagagaagcg ttggcggaat gggcgttgag cgctgtgtct tcattcgtcg
     2341 gggggccgtc cggaggagtc tgttgtttac tgccggagag atttttacga tttgaaacct
     2401 tgtaggatgc ctgaggttga tctacaagaa aagcgtcagc gttcggattg aaataaatct
     2461 ccggatcgta agagaacaga caagtgcgtg ccacatcctg accggaggtg tcgatttcta
     2521 tctgacagag ttcggtgtaa aaagaaacga gtctggtgta agctgcatga tggaagtctt
     2581 ctatttcctg aagggtttcg ggcaatgtct catccggatg gcatgcgcgt actatgattt
     2641 tcacgcccat ccctttgggg ctgataaagg cgatgcgggt gtaatcgcat ctcttgatga
     2701 tagggaggat gcgtgccaat tcttctttgc tgaggtggtc gatgtctatc acgatgtgtc
     2761 ccagatattt gacgaggttc actttggtac gccttcctcg gtagctggcg gagaaggcga
     2821 ttgccggcaa ttcttttttc ttctcattgg cagccttttc tccttgctct gcaaagatgc
     2881 ggcgtatttc ttcgactttt tcccggtagg taaaactgcc gatagtggac actatttctt
     2941 cttcggtcat ctctgctatt gcgtagtaga aaccttgaaa tatggtgaaa tgtttcattt
     3001 ttattttgat tattgtttgt cgatgcaaag gaacttcttt gcggaaatgt gtaaaggaac
     3061 tttgtataaa atggcaataa gttactacgg ttcgcccaaa aagtgtatga tagttgccac
     3121 ttgtttcggc gaaaggagac ggttgcgtgc gcggtagcct tcctgttcga gttcgtgcag
     3181 gagagggagg ttgtaaataa tccaacgacg aagtatttgt aaagcaactt tgaggcagac
     3241 atggggattg taaaggtgag ccagttccgc tttggtatag gtgcggataa ggaaagaatc
     3301 ttctttgtaa ttcatgatgt tttcttcttg ttttatgcta taaagttacg taaaattcct
     3361 tgtttttcaa aggtttgact tcgtttttat tcgttgggaa tcaagaaact tcgttgggga
     3421 atatggcttg tccggctatc cttatctttg tgctgttaac aagaagggga tgcatcctac
     3481 gtattataaa ctttaaaatt attttattat ggcaatgaca gtatcttatt cagtggtgcc
     3541 gcgcaagaat cctgcaaaaa agagtgaacc ggcaaagtat tatgcacagg cgcaggcgtc
     3601 gggtgagctg gactttgaag agttgtgcga agcgatcacc agtcgttcta cttgtacgga
     3661 aacggatgta cgtgcggcta tttcgggtat tctttacgaa gtgaagcgtg cgttgaaggc
     3721 aggaagaatt gcgagactgg gcgatttggg cagtttgcag atagggctga acagtgaagg
     3781 tgcggcatcg gtcaaggaat tttccggttc gatgattaag ggggcgcatc ttattttccg
     3841 tcccggaaag acgctggcgg agttgatgaa gattctgagt tatcagcaag tgttgactcg
     3901 tgcggtggcg caggcgggtg cgggcgatga cgggggagaa gaccctgaca agacccccgg
     3961 ttccggcggt gatggctccg gcgatgaaga ggctccggac cctacggtat aattggcaga
     4021 cgggtcaagg taataacgaa ttaaaataac taccacttaa tattttgata agatgaaaga
     4081 aatagtaaac cggattttag atgtgatcat gtatctgatt cctttcttcg gaaagagaaa
     4141 gcgggacaag gtggtgagag aagtgcgcta tcatactact tgtaaggagg tgtgcaaagt
     4201 gaaaacgacg gaaagggaaa aggaacatga gaaagattag tctgattgtg atccattgtt
     4261 ccgccacccg tgtcgattgc gactttacgg caaaggatgt ggatacggct caccgttatc
     4321 gtggcttttc gtgctggggg tatcattatt atatacgcaa gtcgggcgag attgagccga
     4381 tgcgggatga agatacggtc ggagctcatg cacgtggcta caatgcgatc agcttaggtg
     4441 tatgttatga gggcggtctg gatgagaatg gaaaagcggc ggatacgcgt acaccccgtc
     4501 agaaggaggc gctacatcgt ttggtacacg aattgttgca gcgttatccg gaagctaaag
     4561 tagtagggca tcgggattta agtcccgaca ccaactataa tgggatcgta gatccttggg
     4621 aacggattaa agaatgccct tgctttgagg tcattggaga attctccatt aattaactac
     4681 caccgtagtg aaatactctt tgaatacctc cgcagctttt tcaagctgtt cgggggtatt
     4741 ttcttttacc cctttcatct tgtattcctg tcccatcgct tcgtacttat gtacgcccag
     4801 tgtatggtaa ggcagtattt ccacccgttg gatcatcttg tattttccga gtgcttctcc
     4861 cagtgcgcgt atgtcttctt caaaatcgct gtatccgggc actagcacat aacgcagcca
     4921 gaaaggtttc tcctgttctt ccagccaggc ggccgtgcgg atggtttgct cgttgcttct
     4981 tccggtcagc gtctgatggc ggttgggatt gaattccttt atatccaata acacaagatc
     5041 ggtcagcttg aataattctt ctacgtcttc gttccaaagt cctccgttgc tgtccagaca
     5101 gacatggatg cctttttctt tcagttcgcg gaccagcggg acgagtgctt tcgcctgaaa
     5161 cgtcggttcg cctccggaga aagtgattcc tcctcgtttc ccgaagaagg ggcgctggct
     5221 cattgccatg cggacgattt cttccggtga ggtaggcgtg cctccctttc ctgctatcgt
     5281 atccggattg gcgcaataga ggcagcagaa gttgcatcct tgaagaaaaa cgacgagccg
     5341 taagcccggc ccgtcgaatg ttcccatact ttcgtatgaa tgtacgttta tcatcatata
     5401 gagtaataga ttacatacgt tcgtggaagc tacggctgat aacttccagt tggtgttcgc
     5461 ggctcagctt cacgaagttt acggcatagc cggaaacgcg gatggtcagc tgcggatagt
     5521 tttctggatg ctccatggca tcgtacagca tatcacggtt cagtacgttt acgttcaggt
     5581 ggtgggcacc tttggtgaag taaccgtcca tcatcgtcac gaggttctcg atacggtctt
     5641 cgtccgtagc acccagtgat ttcggaacga tagagaaggt gttgctgata ccgtcctgtg
     5701 agtcacggta acgaagtttc gctacagagc tcagagaagc gatcgcacca ttcttgtcgc
     5761 gtccgtgcat cgggttggca cccggagcga aggcaacacc tttggcacgt ccgtcgggag
     5821 tagcaccggt ctttttgccg tacatcacat tggaagtgat agtcagcaag gagagggtag
     5881 gacgggcatt cttgtaaaca ggcaatttct tcaattcttc gctgaagaag tataccaggt
     5941 ctacgccgag gtggtctacc ttgtcgttgt cgttaccgaa gcaagggaat tctccttcga
     6001 tgtcgaaacc ttctgtcagg ccgatatcgt tgcggcgggc agttactttc gcatatttga
     6061 tggcagacag tgagtcgagt gcgatggaaa gtccggcaac accgtaagca aggtttatgc
     6121 gcgggttcgt atctacaaga gccatctggg ctttctcgta gtaatacttg tcgtgcatat
     6181 agtggatgat gttcatcgct tcgttgtata cgcgggcgat ttctgtcagc actttcttgt
     6241 agttgtccat tacttcttcg aacttcagcg tgtcactggt cagtacgggg atgttcttca
     6301 ccatgactgt accggtattc tcgcaacgtc cgccgttgat ggcaagcaac agggctttgg
     6361 ccaggttgca acgggcaccg aagaactgaa tctgcttacc tatttcctgg tatgatacgc
     6421 agcaggcgat tccgtagtcg tcggactgac gtacttcgcg catcaggtcg tcattttcgt
     6481 actggataga agaagtgtcg atagaaacct ttgcacagaa ttctttgaat ccttccggaa
     6541 gttccgggct ccagagtacg gtcaggttcg gttccggtga agggccgagg ttgtacaatg
     6601 tctgcaggaa acggaaggag gtctttgtca ctttggtacg tccgtcgttg agacgtccgc
     6661 ccagagattc ggttacccag gtcgggtcgc ctgcaaagat gtcgttgtat gattgcatac
     6721 gcaggtggcg gaccatacgc agtttgatga caaactggtc gatcagctct tgcgcgaacg
     6781 attcggtgat ggttcctttg ctcagttcat attccagata gatatcgagg aaagaagaaa
     6841 cgttacccag tgacatagcg gcaccgtctt gttctttgac ggcggcaagg taagccatat
     6901 atacccactg tacggcttct tgtgccgtgt aagcgggacg gctcaggtcg agaccgtagt
     6961 attcgcccat cactttcatg tctttcagtg ccttgatctg ttctgccact tcttcgcgca
     7021 gacggatgcg ggcctctgtc atcggaccgg tgaggttgtg caaatcttcc ttctttgctt
     7081 cgatcagacg gtcgatgccg taaagagcca tacggcggta gtcaccgatg atgcgtccgc
     7141 gtgcatagtt gtcgggaagt ccggtgagga atcccagcga acggaaggaa cggatttctt
     7201 ccgtatatac gtcgaatact ccgtcgttgt gcgtcttgcg gtagtgagtg aaaatatctt
     7261 ttacgcgttc gtccacttcc acaccgtttt cgtgacaggc tttgctgact acgttgatac
     7321 ctccgaaagg cttgatggca cgtttcagaa gttcgtctgt ctgcaggccg acgatcagtt
     7381 cgttttcctt gtcgatatat ccggccttgt gggaagtgat ggttgatacg gtaacattat
     7441 ctaatgagcg gacaccgttg ttttctcttt cttctgccaa cgcttcgagg cagcggttcc
     7501 atacggcttt ggtacgttct gtaggtcctt cgaggaatga agcatctccg taatacggag
     7561 tgatgttatg acttacgaaa tctctgacgt tgatttcgct gctccaaaga ccgtctttaa
     7621 agatcttgtt taattccata aaatgataaa attagaggtt gtagttatcc t
//
