LOCUS       c00157_gnlPro..         4625 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GAHIDPKH_157.
ACCESSION   c00157_gnlPro..
VERSION     c00157_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:53:25
            Original ID  :: gnl|Prokka|GAHIDPKH_157
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4625
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1020)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_1"
                     /sec_met_domain="OAD_beta (E-value: 3e-131, bitscore:
                     425.9, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFVSFLGNNLVDFWGYTGFANATPGHLFMLLIGLFFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMEAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYIIALLIGFEPNQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRALTTKHERLIRMKPPRIVSHTEKVIFP
                     IVGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDVITILLGLTVGAS
                     TQASQFLTWDSILIFILGAFSFIIATASGVMFVKFFNLFLKKGNKINPLIGNAGVSAVP
                     "
     protocluster    1..4625
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4498]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4498
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4625
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4625
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(1023..1454)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 69.7; E-value:
                     4.8e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 3.9e-23, bitscore:
                     69.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQYKYKINGNLYNVTVNDVEDNIANVEVNGTSYKVELDKPVKAA
                     PKPVTRPAAAPKTETGAPVVTKQPTASKKDGVKSPLPGVILDIKVKEGDTVKRGQTIII
                     LEAMKMENNINANKDGKVAEIKVNKGDSVLEGTDLVIIE"
     CDS             complement(1480..2394)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_3"
                     /sec_met_domain="OAD_gamma (E-value: 6.3e-17, bitscore:
                     50.6, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKRIGIFIALLAMVCMGLRAQSATSLRINEVLVVNDQNYQDDY
                     GLHNAWIEIFNTSFASVNLEGCFLTNDKNNPTKYPIPKGDVLTLIKPRQHALFWADGMP
                     NRGTFHVNFTLDPNKENYIALYDSNGKTLIDEVTIPAGQLADCSYAREKDGSANWVVKG
                     EGEHSYVTPSTNNMTIDKNPKIENFKKHDSIGIGMAIIAMSVVFIGLVLLYLSFKAVGN
                     VAVRLGKKNAMKATGITDKTEAKEKNLGSHTGEETAAIAMALHEYLNDAHDVEDMILTI
                     NKVKRTYSPWSSKIYTLRQTPKR"
     CDS             complement(2415..3968)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_4"
                     /sec_met_domain="CT_Btheta (E-value: 1e-291, bitscore:
                     955.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIELRAQARLGGGEKAIEKQHAKGKYTARERIAQL
                     LDEGSFEELDMFVQHRCTNFGQEKKHFLGDGVVTGYGTIEGRLVYVFAQDFTVFGGSLS
                     ETMAQKICKVMDMAMKMGAPVIGINDSGGARIQEGINALSGYAEIFQRNIMASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMTEGTSYMFLTGPAVVKTVTGEDVSQEDLGGASVHAS
                     KSGVTHFTAETGEEGLAIIRKLLSFIPQNNLEEAPLVNCTDPIDRMDDLLNEIIPDSPN
                     KPYDMYEVIGAIIDNGEFLEVQKDYAKNLIIGFARMNGQSVGVVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTV
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVAVLYAKEAKDQENPAQFLA
                     DKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLTNPAKKHGNIPL
                     "
     CDS             complement(4094..4498)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg157_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.1e-24, bitscore:
                     75.4, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGDTKIELLEPTCPESTIAKFIENKGAGVHHVAFAIEDGVANALAEAESKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
ORIGIN
        1 cggtacagca ctcacgcctg cattaccaat cagcggattg atcttattgc ctttcttcaa
       61 gaatagattg aagaatttga caaacatgac acctgacgct gttgcgatga tgaaagagaa
      121 tgcaccgagg ataaagatca agatagaatc ccaggtgagg aattgtgaag cttgggtaga
      181 agctcccaca gtcagtccca acaggatagt gatcacatcg atcaacggac cactggccgt
      241 atttgccaaa cgacgggtta ctccactttc tttcagaaga ttaccgaaga acagcatacc
      301 cagcaaaggc agacccgaag gaaccaggaa gcatgtaagt aacagaccca cgatggggaa
      361 gattactttt tccgtatgcg atacgattct cggtggtttc atgcggatca gacgctcgtg
      421 tttggtggtt agcgcacgca tgataggtgg ctgtatcacc ggcaccagcg ccatgtaaga
      481 ataggctgac acagcgatgg cacccatcag gttgggagcc agtttggacg aaaggaagat
      541 ggccgtcggt ccgtcggcac cgccaatgat accgatagca cccgcctggt tgggttcaaa
      601 cccaatgagc agggcaatga tatacgcgcc gaagataccg aattgcgcag cagctccgat
      661 cagcatcaat ttggggttgg agatcagcgc cgagaagtcc gtcatggctc cgatacccag
      721 gaaaatgagc ggcggatacc agccggaggt tactccttga taaaggatgt tcagtaccga
      781 accttcttcg taaataccca cttttaatcc ggcttccata ttgaatggaa tattaccgat
      841 cagaataccg aagccaatgg ggatcagcag catcggttca aattctttgg ctacggcaag
      901 gtagataaag aagagaccga ttagcaacat gaaaagatgt ccgggtgtcg cgttcgcaaa
      961 accggtgtat ccccagaagt caacaaggtt attccctaag aatgatacga aatctcccat
     1021 aattattcaa tgattacgag gtctgtacct tcaagtacag aatctccttt attaacttta
     1081 atttctgcta cttttccgtc tttattggca ttgatgttgt tttccatctt catagcctca
     1141 aggatgatga tcgtctggcc tctcttcacg gtatcccctt ctttcacttt tatgtcgagg
     1201 ataacgcccg gaagcggaga tttcacaccg tcttttttgg aagctgtcgg ttgtttggtt
     1261 actaccggag cacctgtttc cgttttcgga gcggctgccg gacgggtcac cggtttcgga
     1321 gcggctttga cgggcttgtc caactctact ttataagagg ttccgttcac ttcaacgttg
     1381 gctatgttat cttctacatc gttcactgta acgttgtaaa ggttgccgtt gattttatat
     1441 ttatattgtt tcattgcttc attttattag ggttagtaat tatcttttcg gagtctgacg
     1501 cagtgtgtag atcttcgaac tccagggtga gtaggtgcgt ttcactttgt tgatagtcaa
     1561 aatcatgtct tcaacgtcgt gagcgtcatt caggtattca tgcaaagcca tggcgatcgc
     1621 ggcggtttct tcacctgtgt ggctacccag gttcttttcc ttcgcttccg tcttgtcggt
     1681 gataccggtg gctttcatcg cgtttttctt acccagtctg acagctacgt tacctaccgc
     1741 cttgaatgaa agatacaaga gtaccaaacc tatgaatacg accgacatgg cgatgatggc
     1801 cataccgata ccgatagagt cgtgtttctt gaagttctcg atcttcgggt ttttgtcgat
     1861 agtcatattg ttggtgctcg gagtgacata gctgtgttct ccttcacctt ttactaccca
     1921 gttggcactt ccgtcttttt cgcgagcgta tgaacagtcg gcaagctgac cggcgggtat
     1981 ggttacttcg tcaatcagag ttttaccgtt agagtcatac aaagcaatgt aattctcttt
     2041 gttcgggtcg agcgtaaagt tcacgtggaa cgtaccccgg ttaggcattc cgtctgccca
     2101 gaacaaagca tgttggcgcg gcttgatcag agtcagcacg tcgcctttgg gaataggata
     2161 tttggtcgga ttgtttttat cgttcgtcag aaagcaacct tccagattta cagaagcgaa
     2221 agaggtgttg aatatctcta tccacgcatt gtgcagtccg tagtcatcct gatagttctg
     2281 gtcgttgacg accaaaacct cattgattct cagactggtg gcactttgcg ccctgagccc
     2341 catgcaaacc attgccagca gagcgataaa tatcccgatt cttttcttat tcataacaat
     2401 cgatgtttaa tttgttacaa tggaatatta ccgtgctttt tagcagggtt ggttaatttt
     2461 ttggtctgca gctgttgcag ggcgcggatc acgcggaaac gagtgtttct cggttcgata
     2521 acatcgtcga tgtaaccgta tttggctgca ttgtacgggt tggcgaacag tttagtgtac
     2581 tcggcttcct tgtctgccag gaattgagca gggttttcct gatctttggc ttctttggca
     2641 tacaataccg ctactgcacc ggcaccgccc attacggcga tttcggctgt cggccatgca
     2701 tagttcatgt cgccgcggag ttgcttgcaa ctcattacga tgtgcgaacc accgtaagat
     2761 ttacgcaggg taacagttac cttgggcaca gtggcttcac cataagcgta cagcaacttg
     2821 gctccgtgaa ggattacacc attatattcc tgacccgtac ccggaaggaa tcccggaacg
     2881 tctaccaatg tcaccagcgg aatgttgaat gcgtcgcaga agcgaacgaa gcgtgcacct
     2941 ttgcgtgaag cattgctgtc gagtactccg gcgaggtatt taggctgatt ggcaaccaca
     3001 cctaccgatt gtccgttcat acgggcaaaa ccgataataa gatttttcgc atagtctttc
     3061 tgtacttcca ggaattctcc gttatcgatg atggcgccga tcacttcgta catatcgtac
     3121 ggtttgttcg ggctgtcggg gatgatctcg ttcagcaggt cgtccatacg gtcgatgggg
     3181 tcggtacagt tcaccaatgg agcttcttcc aggttgtttt gcggaataaa gctgagaagc
     3241 ttgcgaataa tcgccagacc ttcttcaccg gtttcggcag tgaagtgagt tacaccggac
     3301 ttgctggcat gtacgcttgc accacccaga tcttcctgag acacgtcttc acccgttact
     3361 gttttcacaa cagccggtcc ggtgaggaac atgtaagatg taccttccgt catcagcgtg
     3421 aagtccgtca gggcgggaga gtaaaccgca ccgccggcac acggaccgaa aatacctgaa
     3481 atctgtggga tgacacccga agccatgatg ttgcgctgga agatttcagc ataaccggac
     3541 agggcgttga tgccttcctg aatacgtgcg ccacccgagt cgttgatacc gataacaggg
     3601 gcacccatct tcatggccat atccattact ttacagatct tttgtgccat ggtttccgac
     3661 agtgaaccac cgaatactgt gaaatcttgt gcgaaaacat atactaatct accttctatc
     3721 gttccataac cggttaccac accgtcgccg aggaaatgtt ttttctcttg tccgaaattg
     3781 gtacatctgt gttgaacaaa catatccagt tcttcgaaac taccttcgtc aagcaactgt
     3841 gcaatacgct cgcgggctgt atatttgcct ttggcgtgtt gtttttcaat agccttttcg
     3901 ccaccaccta aacgagcctg agcacgaagt tcaataagct cttttacttt ttcaagctgg
     3961 ttactcatga gtttttctta atttaaaggg ttataatatt tacaacagga cgattgacaa
     4021 cccggatggg gaatttaatg caaagaaaaa ttccgtttgt ccgtggcgcg gatcgtccga
     4081 atcgtacatc tttttagtgt tcgcagagtt ctgtcagtac gcccagagtt gatttcgggt
     4141 gaaggaaagc aatgttcagg ccttcggcac ctttgcgcgg agctttgtcg atcaggcgga
     4201 tctctttgct ttcggcttcg gccaaggcat tggctacgcc gtcttcaatg gcgaatgcta
     4261 cgtgatgaac acctgcacct ttgttttcaa tgaatttcgc gatggtactt tccggacatg
     4321 tgggttccag taattcgatt ttggtatcac ctactttcag gaaggctgtt cttacttttt
     4381 gatcttctac agtttcgatg ttgtagcatt tcaaacctaa aacgttttca taatacggaa
     4441 gggcttcttc gatacttttt acggcaatgc ccaaatgttc aatgtgtgaa atcttcataa
     4501 ctatattttt aaattttggt attaatcgtc ttttcggaat ggtataaaac agcacaaaga
     4561 aaatcaattc ttgtttaata aagaaattta tctgcaatta attatggtga aaataggttt
     4621 aaaac
//
