LOCUS       c00272_gnlPro..         7937 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JMKKPDAJ_272.
ACCESSION   c00272_gnlPro..
VERSION     c00272_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 10:43:46
            Original ID  :: gnl|Prokka|JMKKPDAJ_272
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7937
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..141)
                     /locus_tag="ctg272_1"
                     /transl_table=11
                     /translation="MEGCIYPFERFERLKKWKGYSLAYSFGYSFKLLQKRNVLIAYSFG
                     YS"
     protocluster    1..7937
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[944:3917]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      945..3917
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7937
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7937
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(209..421)
                     /locus_tag="ctg272_2"
                     /transl_table=11
                     /translation="MSKVIHVHLIFEKKNIYFGSISAIFETLTEKQVGITKSSLLHAGL
                     VDDIAKYTKRAMIIQSRLITCTRKG"
     CDS             945..3173
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg272_3"
                     /sec_met_domain="Gly_radical (E-value: 3.9e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTDARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             3189..3917
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg272_4"
                     /sec_met_domain="Radical_SAM (E-value: 3.4e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3989..4435)
                     /locus_tag="ctg272_5"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVSELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIEEFES"
     CDS             complement(4422..4589)
                     /locus_tag="ctg272_6"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             complement(4610..5113)
                     /locus_tag="ctg272_7"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSVKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             5307..5537
                     /locus_tag="ctg272_8"
                     /transl_table=11
                     /translation="MYYPDQPFHIRTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             5625..7742
                     /locus_tag="ctg272_9"
                     /transl_table=11
                     /translation="MDHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPMFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPADEMDALIGSAYGHNEEFDTRKLNSTQKRILQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSITYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPSNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVNSME
                     FIKINYAQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
ORIGIN
        1 tgaataacca aatgaataag caatcaaaac atttcgtttt tgtaatagct taaatgaata
       61 accaaatgaa taagcaagtg aataaccttt ccacttttta agacgttcaa agcgttcaaa
      121 cggataaata cagccttcca tcatagtttg acacttataa gggcaaaaaa agccgctttt
      181 gcggctttta attgcgttct aaggcatttt atccctttct ggtacatgtt atcaagcgag
      241 actgaataat cattgcacgt ttcgtgtatt tggcaatgtc atcaaccagt ccagcatgta
      301 aaagactact cttagtgatt ccgacctgtt tctccgtcag agtttcaaaa atggccgata
      361 tactaccaaa gtagatgttc tttttctcaa aaatcaaatg tacatggata actttactca
      421 tgatatatag tatttatttc actgcaaata taccaaatat cagctatatg gaataatttt
      481 aataaataaa aataggagag aagcgaagcg ctcccctact ccacttgcat aaattacacc
      541 atttggttat ctttgtatat ggaagtatgg cctgggcaaa gcatcggagt gaaataatac
      601 catactgcct gaattctccc ctactccact cctaatgtaa agagattcat ttgaacggcg
      661 ttcaaacaag gttcaaatgt aagctcgatg taaagcgatg taaacgcttc gtttttccac
      721 ccagctcact cctaccccgt tctaacgctt tgaaaaccaa agcaatcaga tattttcaga
      781 ccgaccgaac tttgacacgc atcgtttctc cccccttaca atatctaggt attttatgtt
      841 gaaaagcata aaatacagca tatttatgct gattatatga aaaaacgttg taactttgtg
      901 cgctctaaag aaaaggataa ctacaacctc taattttatc atttatggaa ttaaataaga
      961 tctttaaaga cggtctttgg agcacagaaa tcaacgtaag agatttcgtt agtcacaaca
     1021 tcactccgta ttatggagat gcttctttcc tcgaaggacc taccgaacgc actaaagctg
     1081 tatggaaccg ctgcctcgaa gcactggcag aagaaagagc caataacgga gtacgctcac
     1141 tggataatgt caccgtctca accatcactt ctcataaagc cggatatatc gacaaagaaa
     1201 acgaactgat cgtcggtctg caaacagacg aacttctgaa acgtgcaatc aaacctttcg
     1261 gaggtatcaa cgtggtgagc aaagcctgcc acgaaaacgg cgtggaagtg gacgaccgtg
     1321 taaaagatat attcactcac taccgcaaga cgcacaatga cggagtattt gacgtatata
     1381 ccgaagaaat ccgttcgttc cgttctctgg gattcctcac cggacttcct gacaactatg
     1441 cccgcggacg tatcatcggc gactaccgcc gtatggctct ctatggtatc gaccgactga
     1501 ttgaggcaaa aaaagaagac ttgcgtaacc ttaccggtcc gatgaccgat gcccgcatcc
     1561 gcctgcgcga agaagtggca gagcaaatca aggcattgaa ggacatgaag gtaatgggcg
     1621 aatactacgg actcgacttg agccgtcctg cctacaccgc acaagaagca gtacaatggg
     1681 tatacatggc ttaccttgct gccgtaaaag aacaagacgg tgctgccatg tcactgggta
     1741 acgtctcttc gttcctcgac atttatatgg aatatgaatt gagcaaagga accatcacag
     1801 aatctttcgc acaggagctg atcgaccagt ttgtcatcaa actgcgtatg gtgcgccacc
     1861 tgcgtatgca atcttacaac gacatctttg ccggcgaccc gacatgggta accgaatctc
     1921 tgggcggacg cctcaacgac ggacgcacga aggtaacaaa gacttctttc cgcttcctgc
     1981 aaacactcta caacctcggc ccttcaccgg aaccgaacct gactgtattg tggagcccgg
     2041 aactgccgga aggattcaaa gaattctgcg caaaagtatc tatcgacact tcttccatcc
     2101 aatatgaaaa cgacgacctg atgcgtgaag tacgccagtc tgacgactac ggaatcgcgt
     2161 gctgcgtatc ttatcaggaa atcggaaaac aaatccagtt cttcggcgca cgttgcaacc
     2221 tggctaaagc cctgctgctt gccatcaacg gcggacgttg cgaaaacacc ggcacggtaa
     2281 tggtgaaaaa catccccgta ctaaccagcg acacactgaa cttcgaagaa gtaatgagca
     2341 actacaagaa ggtattgaca gaaatcgccc gtgtttacaa cgaagcgatg aacatcatcc
     2401 attatatgca tgataagtat tactacgaaa aagcccaaat ggctcttgta gataccaacc
     2461 cgcgcatcaa ccttgcttac ggtgtagccg gactttctat cgccctcgat tcactgtcgg
     2521 ccatcaaata tgccaaagtg actgcacgcc gcaatgacat cggtctgaca gaaggtttcg
     2581 atattcaagg cgaattccct tgcttcggta atgacaacga caaggtagac cacctgggtg
     2641 ttgacctggt ctatttcttc agcgaagaat tgaagaaact tccggtttac aaaaatgccc
     2701 gtcctactct gtctttgctc accatcactt ccaacgtgat gtatggcaag aaaacaggtg
     2761 ctactcccga cggacgcgcc aaaggtgtcg cttttgctcc gggagccaac ccgatgcacg
     2821 gacgtgacaa gaacggtgct atcgcctctt tgagttccgt agcgaaattg cgttaccgcg
     2881 actcacaaga cggtatcagc aacaccttct ccatcgttcc gaaatcactg ggagctactg
     2941 aagaggatcg cgtagaaaat ctggtgacaa tgatggatgg ttacttcacc aaaggcgctc
     3001 accacttgaa cgtaaatgtg ctgaaccgtg agatgcttta cgacgccatg gagcatccgg
     3061 aaaaatatcc gcagcttacc atccgtgttt ccggttatgc cgtaaacttc gtgaagttga
     3121 gccgcgaaca tcaattggaa gtaatcagcc gtagcttcca cgaacgtatg taatcagatc
     3181 tactcaatat gacgataaac gtacattcat acgaaagtat gggaacattc gacgggccgg
     3241 gcttacggct cgtcgttttt cttcaaggat gcaatttccg ctgcctgtat tgtgccaatc
     3301 ccgacacgat agccggaaaa ggaggtacac ctaccccacc ggaagaaatc gtccgcatgg
     3361 ctatgagcca acgtcctttt ttcgggaaac gcggaggagt cactttctcc ggaggagaac
     3421 ctacgtttca ggccaaagca ctggttcccc tggtgcgtga actgaaagaa aagggaatac
     3481 atgtatgcat agacagcaac ggcggtatct ggaacgaaga agtagaagaa cttttcaaac
     3541 tgacggatct tgtattgctg gatattaaag agttcaatcc ggcacgccat caggcactga
     3601 ccggaagaag taacgagcaa accatccgca cggcagcatg gctggaagaa aacgagaaac
     3661 ctttctggct gcgttatgta ttagtgcccg gatacagcga tttcgaagaa gacatccgcc
     3721 gtttaggaga ggctctggga aaatataaga tggtgcaacg agtggaaatc ctgccttatc
     3781 atacattagg cgtacataag tatgaagcga tggagcagga atacaaactg aaagacgtaa
     3841 aagaaaatac ccccgaacaa cttgaaaaag ccgcggaggt atttaaagag tatttcacta
     3901 ctgtggtggt caattagttg aagaagttga gaattgagag ttaactatgc agtataacag
     3961 tgcgcagcca actctcaact ctccattctc aactctcaaa ttcttcaatc acctcaaaac
     4021 agggacattc tttgatccgt tcccaggggt ctacaattcc gttgtagttg gtgtcagggc
     4081 tcaagtcccg gtgaccgacc acctttgcat ccggataacg ctgcaacaac tcgcttacca
     4141 gacgatgcat cgcctctttc tggcgggaag tacgtgtatc cgctgccttt ccgttctcat
     4201 ccagcccgcc ttcatagcac acccctaaac taatcgcatt aaaaccgcgt gcatgagcac
     4261 ccaccgtatc ttcatccctc atcggttcta tctgtcccga cttgcggata taataatgat
     4321 acccccaaca agaaaagccc cgaaagcgat gggcagtgtc cacatccttc gccgtgaagt
     4381 cgcgatcagc acgggtagca gagcagtgaa tcacaatcag actaatcttt ctcatcgtcc
     4441 ttttcccttt ccgttgtttt cacattacac acttccttat gggtagcatt gaagcgcacc
     4501 tctctcacaa ttctgttccg ctttcttttt ccgaagaaag gcacaaggaa catgattaca
     4561 tccaaaatct tagttacaat ttctttcatt tgatctaata ttaaagttat catgctgccg
     4621 gatccggagc ttctccgctt ccaccaccgt ctgaaccgcc accggaacct ttgtcatcgt
     4681 cttctccttc attgccgcta ccgcccgttt gggccaccgc acgcgtcgtt acctgttggt
     4741 agctaagaat cttcgtgatg tccgccaacg tctttccggg acggaaaata atatgggcag
     4801 ccgtaatcag cgaacttgaa aactccttga ccgacacagc tccttcactg ttcaacccga
     4861 tctgcaaact gcccaaatca cccagccgga ctattcttcc cgctttcaac gcacgcttcg
     4921 cctcataaag aatacccgaa atggcggcac gcacatctgt ctccgtacag gtagaccgac
     4981 tcgtgattcc ctcgcacaac tcttcaaaat ccaactctcc cgaagcctgt gcctgcgcgt
     5041 aatactttgc cgactcatcc tttttggcag gattcttgcg cggcactact gaataagtta
     5101 ctgtcattgc cataataaaa taattttaag atttcacaca tttcgaacgc gttccttatt
     5161 tgttaacgac ataaagataa cacatttttc accctccgga ttccccaacg aagtttcctg
     5221 atattctatg aataataatg acgttattct tttaataaac cattaatttt cagtaacttt
     5281 atagcataaa ccaaatatca ttcatcatgt attatccgga tcagccgttt catatccgca
     5341 cttataaaaa gtcggaacta gctcatcttt acaaccccaa tgtatgtctc aaagtagcct
     5401 tacaaatact acgtcgttgg attgtctaca accttccgct cctgcaagaa ctcgaacaag
     5461 aaggctatcg tgcccgcaac cgccttctct ctccccggca agtggcaacg atcatacgct
     5521 atttgggaga accctaggaa cttaatcaaa aaaacgaaaa gttccattct attattttac
     5581 aaacaaattt ctttgcaacc gcaaattaaa aagaacaaac caacatggat cattttacca
     5641 tctttcaaga cttttaccgc gtcatagccg aaatgacaga agaagaaatc gtatcggcca
     5701 tcagcaacgg cacataccgg gaaacggtgg aggacgtgcg ccgaatcttc gcggaacaag
     5761 gcgagaaagc agccaacgaa aagaaaatag aactaccgga aatcaccttt tcagccaact
     5821 accggggagg acgttcaaac gcaactctcg tcaagtatct gggatacatc gtggtagaca
     5881 tcgaccatca gacacaggaa gcattggcac gcatcctcgc actggcaaaa aagtgtgccc
     5941 acacccgcat cgctttcatc agccccaaag gaatgggact gaaaatcgtg gtacgtgtat
     6001 gccggacaga cgggacattg cccgaaacaa ttcaggaaat tgaagatttc catcatgctg
     6061 cttatcacaa aatcgcttcc ttttatgccc agctctgcgg agttgaggtc gacacttccg
     6121 ggcaggatgt tagccgcact tgcctgttct cttacgatcc ggatatttat ttcaatcccg
     6181 atgccacagc tgtcatcgtg gaacagccac cgatgttctt caagtctcaa aagaagaaaa
     6241 gggcatccgg caaaaagaaa aagacgacag caccagacaa caatcccctc accgaacaag
     6301 tggccctcaa ctaccattcc tcgcacgctt ccttaatggt gactctgaac tattatcaca
     6361 ataaatccga aaagtacgtc accggcaacc ggaacaacta cctgcattgc ctggcttgta
     6421 tgtacaatcg ctacggcgtc ccccaggaag aggcggcagc ttttatcaag agccagttca
     6481 ccgatcttcc cgcggatgaa atggacgcac tgatcgggag tgcttacgga cacaatgagg
     6541 aattcgacac ccgcaagctg aatagtacgc agaagcgaat attacagatt gagcaacata
     6601 tcaaagaaaa ttacgataca cgctacaacg aagtgcttca tatcatggaa tatcgcagaa
     6661 ggaaaacgga cacggagcaa ccggaacctt tccatattct cgacgaaatg atggaaaaca
     6721 gcatttggat ggagatgaac gaactgggct attcgtgcac cgtcaagacg attcagaatc
     6781 tgatctactc cgacttcagc atcacctacc atcctattcg cgagtattta gactcgctgc
     6841 ctgaatggga cggaacggat tatatcggta tccttgccaa cagcgtgcat actagtcacc
     6901 agaaattttg ggtagaatgt ctggaacgtt atctggtggg aatgtgcgct gccgccacac
     6961 aagatgatgt ggtgaaccac actgttttgt tgctttgcag cgaaatacag aacatcggaa
     7021 agactacgtt tatcaacaac ctgctgccgc ccgaattgcg cgcctatctt tctaccggtc
     7081 ttatcaaccc gagcaacaag gatgatctag ccaaaatagc gcaggcaatg cttatcaatc
     7141 tggatgagtt tgaagggatg aacggacggg aactgaatat cttcaaggat ttagttaccc
     7201 gcaaagtaat cagcatccgc ctgccatacg cgcggcgttc gcagaacttt cctcacacgg
     7261 cttcttttgc cggcacatgc aattatcagg aagtgcttca cgacaccaca ggtaaccgcc
     7321 gttttctctg ctttcatgtc aactcgatgg agtttatcaa aataaactac gcgcagttgt
     7381 atgcacaaat caagtatctg ctcaacaagc cggggtatca gtattggttc acccagtcgg
     7441 aaaactcacg gattgaagaa aacaacgagg atttcatttt ccactcaccg gaagaagaac
     7501 tggtgttgac acatatccgc aaaccggaac gtttcgaaaa ggtgcattat ctgacggtca
     7561 ctgaaattgc ggaattgatt cgcgaacgga cggggtatca atattctcac ggaaccaaag
     7621 cccaactagg aaaagtgatg tccaaacacg gcttcgaatt tcataaggga aaaaacggga
     7681 gaagatacac tgtattcatt attgatacgg aacaggtgaa atcaaacagg ctatatgaat
     7741 aagatttcat ctccttcgca cgcaaaaagg tgagggttgg tgagggtact tttgcaactt
     7801 ttcattttta tatttcttat ttctattctt ctattttctt ctaaaaaaaa tacgaatgtt
     7861 agaaatttac cctcacctac cctcaccttt ttgcgtgcga aggagatgaa atcttattca
     7921 tatagcctgt ttgattt
//
