LOCUS gnlProkkaCNCIGDAG_442 15755 bp DNA linear UNK 01-JAN-1980 DEFINITION gnl|Prokka|CNCIGDAG_442. ACCESSION gnlProkkaCNCIGDAG_442 VERSION gnlProkkaCNCIGDAG_442 KEYWORDS . SOURCE ORGANISM . COMMENT ##antiSMASH-Data-START## Version :: 7.1.0 Run date :: 2024-11-17 09:45:26 Original ID :: gnl|Prokka|CNCIGDAG_442 NOTE: This is a single region extracted from a larger record! Orig. start :: 0 Orig. end :: 15755 ##antiSMASH-Data-END## FEATURES Location/Qualifiers protocluster 1..15755 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="True" /core_location="[5551:5755]" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="1" /tool="antismash" proto_core 5552..5755 /aStool="rule-based-clusters" /tool="antismash" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="1" cand_cluster 1..15755 /candidate_cluster_number="1" /contig_edge="True" /detection_rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /kind="single" /product="RRE-containing" /protoclusters="1" /tool="antismash" region 1..15755 /candidate_cluster_numbers="1" /contig_edge="True" /product="RRE-containing" /region_number="1" /rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /tool="antismash" CDS 3..176 /locus_tag="ctg442_1" /transl_table=11 /translation="MLSVLAREGRPLRQSLQSVGEKLPLLNVRIKCMEERVLILAVFRP KHWFIRLKLLPV" CDS 173..1354 /gene_functions="biosynthetic-additional (rule-based-clusters) Pyr_redox_2" /gene_functions="biosynthetic-additional (smcogs) SMCOG1175:pyridine nucleotide-disulfide oxidoreductase (Score: 368.6; E-value: 6.4e-112)" /gene_kind="biosynthetic-additional" /locus_tag="ctg442_2" /sec_met_domain="Pyr_redox_2 (E-value: 4.3e-49, bitscore: 158.0, seeds: 64, tool: rule-based-clusters)" /transl_table=11 /translation="MKDATFEERSEFYRNAISVKEAVTSALRNKNYHNLADNPNVTVYT GVGSFVSSDVVSVRTSTEEIMLTSKQIIINTGAETVIPPIEGVAGNPLVYTSTSIMELT ELPRRLVIVGGGYIGLEFASMYASFGSQVTVLESYPELIVREDRDIAASVKETLEKKGI VFRMNAKVQSVKHIEDGALVVFSDSQTGEVVELEADAVLLATGRRPNTKDLNLEVAGVE TDARGAIIVDEYLKTTQPNIRAVGDVKGGLQFTYISLDDYRIIREDLFGDKERTTNDRN PVSYSVFIDPPLARIGLNEEEARKQNLDIIIKKLPVMAIPRAKTLGETDGLLKAVIDKN TGKILGCMLFAPDSSEVINIVAVAMKTGQDYTFLRDFIFTHPSMSEALNDLFS" CDS 1869..1964 /locus_tag="ctg442_3" /transl_table=11 /translation="MDDKLRLLIEEHGGYVTRKEIIGNRYLYEQI" CDS 2560..4071 /locus_tag="ctg442_4" /transl_table=11 /translation="MKIFPSSSIKKLDAYTIENEPIASIDLMERAATVLTKAITDRWNT ETPVTIFAGPGNNGGDALAVARMMAEKGYKIEVFLFNTKGELSPDCQTNKELVEMMEEV KFHEISTQFVPPALTPDHLVIDGLFGSGLNKPLSGGFAAVVKYINSSPAMVVAIDIPSG LMGEENTFNVKANIIRADVTFSLQLPKLAFLFAENTEFVGEWELLDIQLSEDGIEEMET NYEMLEIEEIRSLIKPRKQFAHKGNFGHALLIAGSKGMAGASVLAARACLRSGVGLLTV HAPMCNNDILQTSIPEAIVETDINETCFAVPTDTDDYQAVGIGPGLGRNEETEAALLEQ LEHCQTPVVVDADALNILANHRHTLTHLPKGSILTPHPKELERLTGKCQDSYERLMKAC ELARAAHVHIILKGAYSAIITPEGKCFFNPTGNPGMATGGSGDVLTGVILALLAQGYPT EEAAKIGTYIHGLAGDVAQKKQGMIGMIASDIITCLPTAWRLVSE" CDS 4112..5212 /locus_tag="ctg442_5" /transl_table=11 /translation="MFSKPVKKEPNMKKLIIAAGLLMTTSAYAQTEVLTGVTRGKDYGV VYSLPKTQIELEIKANKVNYTPGEFSKYADRYLRLTNVSADPEEYWELASVKVKSVGVP NSETTYFVKLKDKTVAPLMELTEDGIVKTINVPYSNSSVGKKVAPAPAVLQKKANPREF LTEEILMASSTAKMAELVAKEIYNIRESKNALLRGQADNMPSDGAQLKIMLDNLNAQEE AMTQMFSGTCNKEERTFTVRLTPDKEFNNEVAFRFSKKLGVVANNDLAGTPFYISLKDL KSVKMPQEDGKKKKDLDGIAYNVPGQAMVTLTDGKKKLYEGELPITQFGVIEYLAPVLF NKNSTIKVYFDPNTGGLLKVDREEGK" CDS complement(5552..5755) /gene_functions="biosynthetic (rule-based-clusters) RRE-containing: PqqD_RRE" /gene_functions="biosynthetic-additional (rule-based-clusters) PF05402" /gene_kind="biosynthetic" /locus_tag="ctg442_6" /sec_met_domain="PqqD_RRE (E-value: 6.5e-12, bitscore: 35.9, seeds: 9, tool: rule-based-clusters)" /sec_met_domain="PF05402 (E-value: 4.3e-18, bitscore: 56.2, seeds: 461, tool: rule-based-clusters)" /transl_table=11 /translation="MSKELHVKLEEHGTAVWELIDGKRNVREIIEKLADHFQNEEGYES RVSAYLSQMQKDGFIKLVIPVA" CDS complement(6000..7310) /gene_functions="transport (smcogs) SMCOG1106:major facilitator transporter (Score: 85.5; E-value: 4.7e-26)" /gene_kind="transport" /locus_tag="ctg442_7" /transl_table=11 /translation="MLCGYFLTDVMSPLKPMLEKELLWDSLDYGFFTSAYGWFNVFLLM LIFGGIILDKMGVRFTGMGACLLMVFGCGLKYYAISTTFPEGAMLFGFKTQVTLAALGY AIFGVGVEIAGITVSKIIVKWFKGKEMALAMGLEMATARIGTTLAMVLTVPLADFFGST DESGTFHTNIPAPILFCLIMLCVGTIAFFLYTFYDKKLDASLDAEGLEPEEPFRMKDIV YIITNKGFWLIALLCVLFYSAVFPFIKYAADLMVQKYNVDPKLAGTIPGLLPIGAIILT PLFGSLYDRIGKGATLMIIGSVMLIFVHTMFALPILNIWWFATIIMIILGFAFSLVPSA MWPSVPKIIPEKQLGTAYALIFWVQNWGLMGVPLLIGWVLNTYCKGPVVDGAQTYDYTL PMAIFALFGVLALIVALMLKAENKKKGYGLEEANIQK" CDS 7325..7444 /locus_tag="ctg442_8" /transl_table=11 /translation="MLEHSIGVRRSHSISVSNVLSLLYLKIVVNNLHTTNKGS" CDS complement(7638..8033) /locus_tag="ctg442_9" /transl_table=11 /translation="MYLLFVILMVIAALLMCFIVLIQNSKGGGLASGFSSSNAIMGVRK TTDFLEKATWGLAIFMVVMSIATAYVVPRSAVAKDAVLEQAQKEQQTNPYNLPAGTAAP QTEAPATNAPATETPAPAAETPAPAAE" CDS complement(8040..8813) /locus_tag="ctg442_10" /transl_table=11 /translation="MTSVNFQQWIQHPETLNRDTLYELRNLLARYPYFQSLRLLYLKNL YILHDISFGGELRKAVLYIADRRQLFRLIEGDRYDVQARKKGVPLTEVLKDEPSVDRTL ALIDAFLSTAPEEVTARTSFDYSMDYTSYLLEETPATEQPAEETPKLKGYELIDDFIEK SESDSPLCMKPLREEMPSSPTSSDELTEEETIKEEEEDDSCFTETLAKIYVKQQRYSKA LEIIKKLSLKYPKKNAYFADQIRFLEKLIINANSK" CDS 8849..9313 /locus_tag="ctg442_11" /transl_table=11 /translation="MVGDLFRHFSNQSVLYGSQQLARVVGTERRKLLFKVFGMIGVVDK TYIDGNSQLRLRRVTVGRYCLVVLIVSCDFSVNLQVRILIRLEQLQTGLPDEDIFQVLI EFGCQRSIYILRLDREVGYRNGLYLIIIDRRSVKLIGNSARNDYQRQAEQ" CDS complement(9333..10574) /gene_functions="regulatory (smcogs) SMCOG1112:sigma-54 dependent trancsriptional regulator (Score: 390.2; E-value: 2.3e-118)" /gene_kind="regulatory" /locus_tag="ctg442_12" /transl_table=11 /translation="MTKAEIQQVKQRFGIIGNTEALSRAIDVAIQVAPTDLSVLITGES GVGKESFPQIIHQYSRRKHGQYIAVNCGAIPEGTIDSELFGHEKGAFTGAIGERKGYFG EADGGTIFLDEVGELPMSTQARLLRVLESGEFMKVGSSKVQKTDVRVVAATNVNLTQAI AEGRFREDLYYRLNTVPIQIPPLRERGDDVLLLFRKFSADFAEKYRMPAIQLTEDAKKE LLAYPWPGNVRQLKNITEQISIIETNREISAAILQNYLPAQNTQRLPALMGTRESKGFE SEREILYSVLFDMRQEVAELKKMVHNLMAERAGQVGQVGQMGTVVTTPVVTAHQPSVPA IIHTMQPTVCKDDDDIQDTEEYVEESPLSLDEVEKEMIRKALERHHGKRKSAAKDLNIS ERTLYRKIKEYELD" CDS complement(10584..11678) /locus_tag="ctg442_13" /transl_table=11 /translation="MEDNKIRIGITQGDINGVGYEVILKTFSDPTMLELCTPIIYGSPK VAAYHRKALDIQANFSIVNSATEAGYNRLSVVNCTDDEVKVEFSKPDPEAGKAALGALE RAIEEYREGLIDVIVTAPINKHTIQSEEFSFPGHTEYIEERLGNGDKSLMILMKNDFRV ALVTTHIPVREIATTITKELIQEKLMIFHRCLKQDFGIGAPRIAVLSLNPHAGDGGLLG MEEQEVIIPAMKEMEEKGILCYGPYAADGFMGSGNYTHFDGILAMYHDQGLAPFKALAM EDGVNYTAGLPVVRTSPAHGTAYDIAGKGVACEDSFRQAIYVAIDVFRNRQREKVARAN PLRKQYYEKRDDSDKLKLDTVDED" CDS complement(11683..11997) /locus_tag="ctg442_14" /transl_table=11 /translation="MSTDSLLTDVRPINVQNSYTMEARGLWRMKGDFMGGPYVSHTRLD EKNQRIITAEIFVYSPDKMKRNLVRQMEASLYTLKLPNEVQQKQIPLGEASKEAEQTNK " CDS complement(12742..13776) /gene_functions="biosynthetic-additional (rule-based-clusters) PF04055" /gene_kind="biosynthetic-additional" /locus_tag="ctg442_15" /sec_met_domain="PF04055 (E-value: 3.6e-18, bitscore: 57.2, seeds: 518, tool: rule-based-clusters)" /transl_table=11 /translation="MSKYPLLGMTLVELQSLTKRLGMPGFAAKQIASWLYEKKVASIDD MTNLSLKHRELLKQNYEVGAEAPVDEMRSVDGTVKYLYKVGENHFVESVYIPDDDRATL CVSSQVGCKMNCKFCMTGKQGYTANLTASQIINQIHSLPERDKLTNVVMMGMGEPLDNL DEVLKALELLTATYGYAWSPKRITLSTVGLRKGLQRFIEENDCHLAISLHSPLTVQRAE LMPAEKAFSITEMVELLKNYDFSKQRRLSFEYIVFKGLNDSQVYAKELLKLLRGLDCRV NLIRFHAIPGVDLEGADMDTMTRFRDYLTSHGLFTTIRSSRGEDIFAACGMLSTAKQEK NNES" ORIGIN 1 tattattatc ggttttggca agggagggaa gacccttgcg gcagagtttg caaagcgtgg 61 gcgaaaagtt gccattgttg aacgttcgga taaaatgtat ggaggaacgt gtattaatat 121 tggctgtatt ccgaccaaaa cattggttca tcaggctaaa attgcttccg gtatgaaaga 181 cgctacattt gaagaaagga gtgagttcta tcgcaatgcc atttctgtga aagaggctgt 241 gacaagtgca ttgagaaata agaattacca taatctggca gataatccca atgtaacagt 301 ctatacaggt gtgggctctt ttgtctcttc cgatgtggtt tctgtccgta catctactga 361 agaaataatg ctgacatcga agcaaattat tatcaatacg ggggcagaaa ctgttattcc 421 tcccattgag ggagttgcgg gtaatccgtt ggtttataca agtacctcaa tcatggaatt 481 gaccgagctt ccccgccggc tggttattgt cggtggcggt tacatcggtt tggagtttgc 541 gtctatgtat gcttcgttcg gttcacaggt gaccgtactc gaaagttatc ctgaactgat 601 tgtgcgtgaa gaccgggata ttgctgcaag tgtaaaagaa acattagaga aaaaaggcat 661 tgtttttcga atgaatgcaa aggttcaatc tgtgaaacat attgaagatg gagcactcgt 721 tgttttctct gattcacaaa ctggcgaggt ggtggaactt gaagctgatg ctgtattgct 781 ggcgacagga cgccgaccga acacgaaaga tctgaatttg gaagttgccg gagtggagac 841 ggatgcacgc ggagcgatca ttgtggatga atatctgaaa acaacccaac cgaatatacg 901 tgccgttggt gatgtgaaag gtggtttgca gttcacttat atttcattgg atgattaccg 961 gattatccgg gaggatctgt ttggcgataa agaacggaca acgaatgatc gcaatccggt 1021 tagctactct gtttttattg atcctccatt agcacgtatc ggattgaatg aagaagaggc 1081 tcgtaagcag aatttggata ttattatcaa gaaattgccc gttatggcga taccaagagc 1141 gaaaacattg ggagaaacgg atggcctttt gaaagctgtt attgataaga atacgggaaa 1201 aatattgggc tgtatgctgt ttgcgcctga ttccagcgaa gtaatcaata tagttgctgt 1261 tgcaatgaag acaggtcagg attatacttt cctgcgtgac ttcattttta ctcatcctag 1321 tatgagcgag gcgttaaatg atttgttttc atagtagtaa gaatgagtat aatcttcctc 1381 tataaagttt aaaaaacatc aaagtatctg ccacctgcaa caaaacgctt gtaatgtatt 1441 agtaaatagg cagttggagc gttgcagata cctgttgcag atacgttgca gataaagtat 1501 ctgcaacgca taatataact aaagaaaagt ccttgatttc tttatgcaaa gctatgggaa 1561 gttcatggta tccctataag gagactgcag tatctacgta cggaaactgc ggtatccata 1621 catggacact gtcgtttcct gccttggaca ctgtcgtttc ctacggtggg aactggagtt 1681 tcttgcctcg gaactgtagt ttcttatgct ggaaactgaa gtttgcttgc ttggaactat 1741 aatttctttt gcttgaaaaa tagttaagtc atgctcctac tcacttttgt atcaaaagaa 1801 aatatgtaca aaactacggc acttagaaat aaatgccgta tatttgtaca taataagaca 1861 agtgtgatat ggatgataaa ttgagacttc tgatagaaga acatggagga tatgtcaccc 1921 gtaaagagat tatcggtaac cgttatctgt atgaacaaat ctaattgctt gaaacatatt 1981 gctttaagct tgtgagcaaa gtcttctaat tatagaatta agaagttcga ctaaaatcaa 2041 gacgcttatg acaaggtatg agggtaaaag ttactctcat cttactctca tgcttactct 2101 catacctgta actccgatga ataaaggctt ttcaaacgat tgatgagagt atgagagtaa 2161 aattgcttat ttatttggtt cttcgtcaaa tttggtggta agtttttctc cgctcattta 2221 gctaattcag tcagtgccta tattttctta tgaaagtgag taaaaaggga taattattaa 2281 cttggaaata aagagataaa gaatttacca ccaaatttga cgaagaacca gaaatctttt 2341 tcaggagaaa taaaagtaag ttcataatgg tgctgcattg aattattttt tggcggagtt 2401 attctaacac ttaatagttc aaaagcagat atttctctga cccaaagata gaactaatga 2461 tgaattaaaa gttgcaatta tggttttctt cccacaacaa acgggaaaag tcagaagaat 2521 catgtacatt tgtaatccga aaactaaaag aacatcgcaa tgaaaatatt tccaagcagc 2581 agtatcaaga aattggacgc ttataccatt gagaacgaac ctattgcatc aatcgatctg 2641 atggaacgtg cagcaaccgt attaacaaaa gccatcacag acagatggaa tacggaaacg 2701 ccggtcacta tctttgccgg accggggaat aatggcggag acgctctcgc cgtagcccgc 2761 atgatggctg aaaaaggata taaaatcgag gtatttctct tcaacacgaa aggagaactt 2821 tcacccgact gccagaccaa taaagaactg gtagaaatga tggaagaagt aaaattccac 2881 gagatcagta cccaattcgt acctcccgcc ctgacgcccg accatctggt aatcgacgga 2941 ttattcggtt caggcctgaa caaaccgtta agcggcggat tcgctgccgt agtgaaatac 3001 atcaattcct cccctgctat ggtagtagcc atcgacattc cttccggact gatgggagaa 3061 gaaaatacat tcaacgtaaa agctaatatt atccgcgccg acgtaacttt cagtctgcaa 3121 ctgccgaagc tcgcctttct tttcgcagaa aacacagaat ttgtcggaga atgggaattg 3181 ttggatatcc aattgagcga agacggtatt gaagagatgg agactaacta cgaaatgttg 3241 gaaatagaag aaatccgttc actcatcaag ccacgcaaac agtttgccca caagggaaac 3301 ttcggtcatg cactattgat tgccggttca aaaggaatgg caggagcctc tgttctcgct 3361 gcccgtgcct gcctccgttc gggagtaggg ttgctgactg ttcatgcacc aatgtgcaac 3421 aacgacatct tgcagacctc catacccgaa gcaatcgtag aaaccgacat caacgaaacc 3481 tgttttgccg tacctaccga cacggacgac tatcaagcag tcggcatcgg tccgggactg 3541 ggacgaaacg aggaaacgga agctgcctta ttggagcaac tggaacattg ccagactcct 3601 gttgtagtgg atgccgacgc acttaatatc cttgccaacc accgccatac ccttacccac 3661 ctgccgaagg gttccatcct caccccgcat ccgaaagaac tggaacgtct gaccggcaaa 3721 tgccaagact cttacgaacg tctgatgaag gcttgcgaat tggctcgtgc cgcccatgtt 3781 cacattattt taaaaggagc ctattccgcc attatcaccc cagaaggtaa atgtttcttc 3841 aacccaaccg gaaatccggg aatggcaacc ggaggtagcg gtgacgtact gaccggagta 3901 atcctggcac tgcttgccca aggttatccc accgaagaag ccgctaaaat cggcacttac 3961 attcacggat tggcgggcga tgtcgctcaa aagaagcaag gaatgatcgg aatgatcgca 4021 agcgatatta tcacctgtct gccgacagca tggcgacttg taagcgaata aaagttaaga 4081 acttacagtt ttttcaataa cttcattaac tttgttttca aaaccagtta aaaaagaacc 4141 gaatatgaaa aagttaatta tagcagcagg actacttatg acaacgtccg cctatgcaca 4201 aaccgaagta ctgacagggg ttacccgagg aaaagactat ggagtggttt acagtcttcc 4261 caaaacccaa atagaacttg aaataaaagc caacaaagtc aactatactc cgggagaatt 4321 cagcaaatat gctgatcgtt acctgcgact gaccaacgtc tccgccgatc ctgaagagta 4381 ctgggaactg gctagcgtaa aagtgaaatc cgtaggtgtg cccaacagcg aaactacgta 4441 tttcgtaaaa ttgaaggata agacagtagc accgcttatg gaactgacag aggatggcat 4501 tgtgaaaacg atcaatgttc cttacagcaa tagcagtgta ggcaaaaaag ttgctcccgc 4561 tcccgcagtc ctccaaaaga aagcgaatcc tcgcgaattc ctgaccgagg agattctgat 4621 ggctagctct accgccaaga tggccgagct ggtggctaaa gaaatctata acatccgcga 4681 aagcaaaaat gccttattgc gcggacaggc tgacaatatg ccttcagacg gagcacagct 4741 taagattatg ctcgacaatc tgaatgcaca ggaagaagcc atgacgcaaa tgttctccgg 4801 cacctgcaac aaagaagaaa gaacattcac tgtccgcctt acccctgaca aggaattcaa 4861 caatgaagtt gctttccgct tctcgaagaa gttaggtgta gttgccaaca atgatttggc 4921 aggaactccg ttttatatca gtctgaaaga cctgaaatct gtcaagatgc cacaggaaga 4981 cggtaagaag aagaaagatt tggatggaat cgcctacaac gtacccggac aagctatggt 5041 tacactgacc gacggtaaaa agaaacttta cgaaggtgag cttcctatta ctcaattcgg 5101 tgtgatagaa tatctggctc cggttttgtt caataagaat tctaccatca aagtttattt 5161 tgatccgaat acgggaggat tgctcaaagt ggacagagaa gaagggaaat agaaaaacaa 5221 ggaagtgaac ttctaaaaaa caaagaatat ggttggtgaa ggacatgcat tcgacatgat 5281 acgccgaata aaagagggac gggaagcttc ccgcctacgt cgtgaacgtg ctaacgataa 5341 gttgaagcat ctaaaccgtg caaacgaacc ttatccacta cccaacacca ctcccgaaga 5401 aatggaacgt atcatccacg actcggagaa aaagaaagag aaagacagca attattttgt 5461 ttggggaacg cttattatca tgggggtcct gatagcaatt gccgtcatac tttgggctgt 5521 atttatcaaa taaataataa taagttccta tttatgcgac tggtatcacc agttttataa 5581 atccatcttt ctgcatttga gaaagataag ccgatacacg cgactcgtag ccctcttcat 5641 tttgaaagtg gtctgcgagt ttttctataa tctcacgaac attgcgtttt ccatctatca 5701 gttcccatac agccgtaccg tgttcttcca atttcacatg aagttctttc gacattcctt 5761 tcggcacaag gaagcgttgc atccacggat atttaaaacg gggaaaggag agcacaattg 5821 tctccccttc cctttctgct gtaatatgtt cactgcgaca gggaatgacc tctaacaggt 5881 tgattttctc tttggcagcc atacttctta ttttttattt cagagtattc atactgatac 5941 ccttttgttt acaccttgat acaaaattcc tttgttactt taggtattga atttcctagt 6001 tacttttgga tattcgcctc ttccagaccg tatcccttct tcttgttctc tgccttcaac 6061 atcaaagcca caatcagagc aagcacacca aagagagcaa aaatagccat cggcaacgtg 6121 tagtcataag tctgtgcacc gtctactacc ggacctttgc aataagtatt caacacccat 6181 ccgatcaaca aaggaacacc catcaatccc cagttctgta cccagaaaat caatgcgtat 6241 gcggtaccca actgtttttc cggaataatc ttcggaacag acggccacat agctgaaggc 6301 accaacgaga aggcaaatcc caatataatc atgatgatag tagcaaacca ccatatattc 6361 agtataggca gagcgaacat cgtatgcaca aaaataagca tcactgatcc gattatcatc 6421 aacgtagctc ccttaccgat acggtcataa agcgagccga acaacggagt caggataatc 6481 gcaccaatcg gcaacaatcc gggaatcgtt cccgccaact tcggatctac attatacttc 6541 tgcaccatca agtcggcagc atacttgata aaggggaata cagccgagta gaacaataca 6601 catagtaatg caatcaacca gaatccttta ttggtaatga tatacacaat atctttcata 6661 cggaacggct cttccggctc caatccctct gcatccaaag aagcatccaa cttcttatca 6721 tagaaagtat aaaggaagaa agcaatcgta cccacgcaca acataatcag acaaaacaga 6781 ataggagcag gaatattggt atggaatgtg ccactctcat ccgtagatcc gaaaaagtcg 6841 gccaaaggaa ccgtaagcac cattgccaga gttgttccga tacgtgccgt agccatttca 6901 agtcccatag ccaatgccat ttccttaccc ttgaaccatt ttacgataat cttggaaact 6961 gtgataccgg caatttccac acctacaccg aagatagcat accccaatgc agccagggtt 7021 acctgtgttt tgaatccgaa gagcatggca ccttccggaa aagtagtaga aatcgcatag 7081 tatttcaatc cacaaccaaa caccatcaat aagcaagccc ccattccggt gaaacgaact 7141 cccatcttat ccaaaatgat accaccgaag atcagcatca acaggaatac attaaaccac 7201 ccgtaagcgc ttgtgaagaa accataatcc agactgtccc atagtagttc tttctcaagc 7261 atgggtttta gaggagacat tacgtcggtg aggaaatagc cgcatagcat agtaaatgcg 7321 actaatgcta gaacactcca tcggagtacg gcggagtcat tcaatttctg tttcaaatgt 7381 tctgtcattg ttatatttaa aaatagtagt taataatctt catacgacca acaaaggtag 7441 ttaaatctcg tttgtttcta tcaaaaagag attgttttta caagaaaaag ttaagtgcgg 7501 attacactga ccacgcggaa gattgaaaat ccgtgtaatc tgcgtaatcc gcacttaaat 7561 atcaaagaaa tgcagcattc gtctgcacct tcccattaat ggttttaccc caaagtctta 7621 cctgacgaca gagtgaatta ctctgctgca ggagcaggag tttcagcagc cggtgccgga 7681 gtttctgtag caggtgcatt ggtagcagga gcttcagttt gcggcgcagc cgtacctgcg 7741 ggtaagttat acgggttagt ctgttgttct ttctgtgcct gttccaacac tgcgtcttta 7801 gcaacagcag agcgaggaac tacataagca gtagcaatgc tcattaccac catgaagata 7861 gctaagcccc atgttgcttt ttccagaaaa tctgtagtct tgcgcacgcc catgatggcg 7921 ttagatgaag agaaaccaga agcaagtccg cctccttttg agttctgaat cagcacgatg 7981 aaacacatca acaaggctgc aataaccatt aagataacga ataataagta catttttagt 8041 tattttgaat tagcgttaat aatcaatttc tccaaaaatc tgatttggtc tgcaaagtaa 8101 gcattttttt ttggatattt caagcttaat tttttaatta tttcaagagc ttttgaatat 8161 cgttgctgtt taacatagat ttttgccaaa gtttccgtaa aacagctgtc atcttcctct 8221 tcttccttta tcgtttcttc ttcggtaagt tcgtccgaag aggttggcga agatggcatt 8281 tcttctctta aaggtttcat gcagagggga gagtcgctct cgctcttttc tataaagtcg 8341 tcaatcagtt cgtagccttt gagtttgggc gtttcttcgg ccggctgttc cgtggccgga 8401 gtctcctcca aaagataaga ggtgtaatcc atcgaatagt cgaagctggt gcgggcagtc 8461 acttcttcgg gggctgtgga caagaaagcg tcgatcaatg ctaatgtgcg gtctacagat 8521 ggttcgtctt tcagtacctc cgtgagaggt actccctttt tccgtgcttg cacatcatag 8581 cggtcacctt caatcaaccg gaacaactgc cgtctgtctg caatgtaaag aactgctttc 8641 cgcagttctc caccgaaact aatatcatgg agaatataca gattcttcag atacagcaga 8701 cgaagtgact gaaaatacgg atacctcgca agaagattgc gcagttcgta caaagtatcc 8761 ctattcagcg tctcgggatg ctgaatccat tgttgaaagt taacagaagt catttatatt 8821 accagtttgc tacagttgca ttaaaaattt ggtcggtgat ctctttagac atttcagcaa 8881 tcaatccgtc ctgtacggca gtcagcaact ggctagagtc gtaggtacgg aacgcagaaa 8941 actgttgttc aaagtcttcg gcatgattgg tgttgttgac aaaacgtaca ttgacggtaa 9001 tagtcaactt cgtctccgaa gagtaaccgt cggcagatac tgcctggttg tactgattgt 9061 atcctgtgat ttctccgtca atctgcaagt ccgcattctg attcggcttg agcagttgca 9121 gacgggtctg ccggatgaag atatctttca ggtcctcatt gaatttggtt gccaacggag 9181 catatacata ctccgacttg atcgggaagt cggctatcga aatggtcttt accttatcat 9241 aattgataga cgatccgtta aacttatagg aaacagtgca cgcaatgact accaacggca 9301 agccgaacag taacaaaggg cgtattattt tcttaatcca attcatattc ttttattttt 9361 ctataaagtg tacgctcgga aatattcaag tccttggccg cactcttccg ctttccatga 9421 tgcctctcaa gtgccttgcg tatcatttct ttctccactt cgtccagtga caaaggagat 9481 tcttctacat attcttccgt gtcttgtatg tcatcatcat ccttgcaaac cgtaggctgc 9541 atggtgtgaa taatagcagg taccgaaggt tgatgcgccg tcactaccgg agtagtaacc 9601 accgttccca tttgtcctac ctgccccacc tgtccggcac gttccgccat caggttgtgc 9661 accatcttct tcagctccgc cacttcctgg cgcatatcga acagaacgga ataaagaatc 9721 tcccgttcgc tctcaaagcc tttactctca cgcgttccca tcagcgcggg aagacgttgc 9781 gtattttgtg cgggaagata attctgcaag atagcagcag agatctccct attggtttca 9841 atgatagata tttgctcggt gatgttcttc aactgacgca cgtttccggg ccacgggtaa 9901 gctaacaact ctttcttcgc atcctccgtc aactgaatag caggcatccg atatttctct 9961 gcaaagtctg cggaaaactt acggaacaac aaaagtacat cgtcaccacg ttcacgcaaa 10021 ggcggtattt ggataggcac cgtattcaac cggtaatata aatcttcacg gaaacgtcct 10081 tctgcaatcg cttgtgtcag attgacgttg gtagctgcca ccacacgcac atccgttttt 10141 tgtactttgg acgaacctac tttcataaac tcgccactct ccagtacacg gagcaaacgt 10201 gcctgggtag acatcggcaa ctcccctact tcatcaagaa aaatagtacc accgtcagct 10261 tcgccaaagt accctttccg ttcgccaatc gcaccggtaa acgcaccttt ctcatgtccg 10321 aacagttcgg aatcaatcgt gccctccgga atagcaccac agttgacagc aatatactgt 10381 ccgtgcttcc tccggctata ttgatgaata atctgcggaa aactttcttt tccgactcca 10441 ctttctccgg taatcagcac agacaaatcg gtaggagcta cctgaatggc aacatctatg 10501 gcgcgcgaca atgcttcagt atttccaata ataccaaaac gctgtttcac ttgttgtatc 10561 tctgctttcg tcatcactcg tttttaatct tcgtctactg tatccagctt cagtttatca 10621 ctatcgtcac gtttctcgta atactgttta cgcaacggat tggcacgagc cactttttca 10681 cgctgacgat tacggaacac gtcgatggca acatagattg cctggcggaa agaatcttca 10741 caagccactc ccttgcctgc aatatcgtat gccgttccat gagccggaga agtacgtacc 10801 accggcaggc ctgccgtata gttcaccccg tcttccatag ccaaggcctt gaacggagcc 10861 agcccctgat cgtgatacat cgccaggata ccgtcaaaat gagtataatt gcctgaaccc 10921 ataaagccgt cggccgcata aggaccgtag caaaggattc ctttctcttc catttctttc 10981 atggcaggga taatcacttc ctgctcttcc attcccaaca aaccaccgtc tccggcatga 11041 ggattgagag agagcaccgc aatacgcgga gcgccgatac cgaaatcctg tttcagacaa 11101 cgatggaata tcatcagttt ctcctgaatc agttctttgg taatcgtagt cgcgatctct 11161 ctgacaggga tatgtgtagt caccaaagcc acccggaagt cattcttcat caaaatcatc 11221 aatgacttat caccattgcc cagacgttct tcgatatatt ccgtatgtcc ggggaaggag 11281 aactcttccg actgaatcgt atgtttgttg atcggagctg ttacgataac gtctatcaac 11341 ccttcgcgat attcttcgat ggcacgttcc aaagccccca gcgcagcttt accggcttcg 11401 gggtcgggtt tggaaaattc cactttcacc tcatcatccg tacagttcac cacgctcaaa 11461 cggttgtacc ccgcttcggt agcagagttg acaatgctga aattggcctg tatatccagc 11521 gctttgcgat gataagccgc tactttcggt gagccataaa tgatcggagt acatagttcc 11581 aacatcgtag ggtcggaaaa tgtcttaaga atcacctcat agcccacccc gttaatatct 11641 ccttgggtga tacctatcct tatcttgtta tcttccatgt ttttatttat tagtttgttc 11701 cgcttcctta gatgcttctc ccaatggaat ctgcttctgc tgaacttcat tcggaagttt 11761 cagtgtatac agagatgctt ccatctggcg taccaggttg cgtttcattt tatcaggtga 11821 ataaacaaat atttctgcag taataattcg ttggttcttc tcgtccagac gagtatgaga 11881 tacatacgga ccacccataa agtcgccctt catacgccac agaccacgtg cttccattgt 11941 gtagctgttc tgcacattga tcggacggac atcggtcagc aaactatcgg tcgacatata 12001 aacgccttcc ttgaatccgg ggatattggc tttcatcacg gagtcacgtt tgtgcacaaa 12061 atattctttg gtaaacgtat ccttgtcagt gtacggataa gagtacatca caaagttacg 12121 gtcggcacta cctgtgttgg tagaagccca gaagaaatca tccccggtct ttgaattagc 12181 cagttcggca tccacccaga tgtcgcatcc gaacaaactg tccactttct gcgaaatgaa 12241 attactgtgt ttcccttcca ggaatgtaat ctgacggttc atttccgcac gggtaaagaa 12301 gtcgacaatc gtctttttgt tctcttccac aaacttctca aactcttctt cgttcggagc 12361 ctggatagtc aatatcatct gcggattggc atacacgtcc ttcgcatatt tgaacgaggc 12421 tttcgtatat atatcctgta tatctacaat aataatgtta cgtatcaact tcagcgtaga 12481 atcataatct ttcggcgaag tatacataat ccggaaagaa ggttccgact gcggcaaacc 12541 gggcatatca gcatcgagcg catcatgtaa agcccttccg gcagcacgat cccaaacacc 12601 gtgatctact acaaccaaaa gctcgtaagc acgaccacta gaggtgtgat ttcctttcag 12661 accacaagat gcaacgacaa aggctaccaa agcgatgcat aaaataaaag agtacttttt 12721 catgatgttc ggttttatat attaactttc attatttttc tcctgtttcg ctgtcgacag 12781 cataccgcaa gcggcaaaaa tatcttcgcc tctggatgaa cggatagtgg tgaaaagtcc 12841 gtgtgacgtc agataatcac gaaaacgggt catcgtatcc atgtccgccc cctcgaggtc 12901 tacccccggt atggcatgaa aacgaatcag attcaccctg caatccagcc cgcgaagaag 12961 tttcagcaac tctttggcat agacctgcga atcattgagc cctttaaaaa caatatattc 13021 aaacgaaagt cgacgttgtt tactaaaatc atagtttttt aacagttcca ccatttcggt 13081 gattgaaaac gccttttccg ccggcattaa ttcagcacgc tgaacggtca gcggcgagtg 13141 aaggctgatg gcaaggtgac agtcattttc ttcgataaaa cgctgcaatc ctttccgtag 13201 cccgacagta gaaagcgtga tacgcttcgg actccatgcg taaccgtagg tagcggtcag 13261 tagttccaat gccttcaata cctcatccag attatcgagc ggctcgccca ttcccatcat 13321 tacgacatta gtcagtttgt cccgttccgg cagcgaatga atctggttta tgatctggct 13381 ggcggtcagg ttagctgtat acccctgttt gccggtcata cagaacttac agttcatctt 13441 gcaacctact tgtgacgaca cgcacaacgt tgcccggtca tcatccggta tataaaccga 13501 ctctacaaaa tggttctcac ccactttata aagatacttc accgtaccat ctacagagcg 13561 catttcatct accggagctt ccgctcccac ctcatagttc tgcttgagca actcccggtg 13621 tttcaatgac aaattagtca tatcatcaat cgaggctacc ttcttttcgt aaagccagga 13681 tgcaatctgc ttggcagcaa agccgggcat acccagtctt tttgtcaatg attgaagctc 13741 tacaagagtc attcctaaaa ggggatattt agacattctt ctttctgttg atagtttata 13801 tctgcaaatg taaggaaata aagtcgattt ccgtatagca ggaatgtaaa agttaacaaa 13861 aaagggaagt atgcctccgt tatgattcaa ccacgctgat atattgaagt agcttttgcg 13921 ccatttcaga atcctcgcca ttctttttca cttcatgacc gtacgcctca cggaaacaag 13981 ccaacgcctc ttcccgttca ttacaaaagc tgtgcaaaac ccctaagttc tgacaagcca 14041 gaatcgtatc ttcttcctcc catcccaaat acttctttcg caattcaagt tctctgaaga 14101 aagcctcttt cgccattgga taattctcca acttcacata agcctgtgct ttgtaataat 14161 ataccagaca ggaagcatta gggtcagcat catcgtgaat atccaaagac gtcgcaatac 14221 gaatcacgtt atcatagttt ttattcatgt aatactgctg cataatatca catctcaatg 14281 cctcgtagcg gacagtcagt tccgccagac gcggtttttc gctgaaatac atttcatgca 14341 gaagagactc acggaaaaat aaagtagacg cctgctcatc gccacgttca ttcatcatcc 14401 gtttgaattc cctgcataga taatacaact gtttatgaca agcgacgatc tgttttttgc 14461 tgtctatctg tatagcaatg tccaacgcct gattcatcag catccatgct tcatcgtatc 14521 gtttgatata ataaagacat ttaccctgca tacgcaaaca tttagccact ccaccggaca 14581 gtctgccatt caatgactcg tgcatttcag ctactttccg gaatttctcg gcagcactct 14641 cttcctcatc cttatagtaa tcacaataag ccagatattc ggaaagttca atgacttctg 14701 cctgactctt ctcgtcagca gaagccgcat gataatcaag cgccctattc aaataggtca 14761 ctgcttcatt gtattgctca ttgtagtaat atgccagagc tagatttcgg caggtgttgg 14821 caatctccag ttccggagtc ggtacaatgt tttcctgcat caccaatgct ttcaagaaac 14881 agtcgcatgc cgtttcgtac tcttcattac gggcatagcg gcatccctca ttcatcttca 14941 ataccgactt ggcaatgagt ggcatggcag caccttccat actcttctca ctcacagcag 15001 ctgccgcctt cgtcagatgg aaagaactgc tcaagaaagt agccatatca ttagcaatga 15061 caggaattaa aaaagaatcg atctcatcaa agtccaattc ggcatagact tcaggcgtaa 15121 cttcaggatt gtcgttcatg atcgccttcc agctatcttc caacagagaa cgattcgtgc 15181 gatacagaat ctcgaaaatc tccacatcac aaatccaatc cttcaatata tcccattctt 15241 gcatcttata gcattgatag gtaatctcca ctttatagcg atacgaatca tggtagcaga 15301 actgtttcat gcgatagtta taatcggcaa aagtcaacgg atgagaaagg atctcttcct 15361 catccagcaa agcggaaata atttcatggc gactatcatc tatacattcg tcatcttgag 15421 cgaaatagcg ttcgatcgcc tctaccattt gtgtgtcacc aatgcaatat cgccctcctt 15481 tgttggtgag ccagctgctc atttccactc tcaactgcga ccaaagtatc tgattaatgt 15541 ctgccatact cttcacttca tcttcggtaa atccttctaa agtaagtgaa agcatcagaa 15601 gcgttcttcc aatctcttcg aaaccataat cagccgacaa acgccgtaac acactgtcat 15661 agaattgccc aacctcattg agccggcaat atccactcat atattcattg agagcatcgt 15721 actgaccgaa cgaaaccagc tcgttgagca ataca //