LOCUS       c00265_gnlPro..         7253 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CNCIGDAG_265.
ACCESSION   c00265_gnlPro..
VERSION     c00265_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 06:50:30
            Original ID  :: gnl|Prokka|CNCIGDAG_265
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7253
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7253
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[354:3327]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      355..3327
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7253
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7253
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             355..2583
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg265_1"
                     /sec_met_domain="Gly_radical (E-value: 3e-47, bitscore:
                     148.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSTEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERANNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLRNLTGPMTDARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYMEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     NFEEVMSNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIQGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATEEDRVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLYDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2599..3327
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 74.2;
                     E-value: 4.3e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg265_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-30, bitscore:
                     94.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGVTFSGGEPTFQAKALVPLVRELKEKGIHVCIDSN
                     GGIWNEEVEELFKLTDLVLLDIKEFNPARHQALTGRSNEQTIRTAAWLEENEKPFWLRY
                     VLVPGYSDFEEDIRRLGEALGKYKMVQRVEILPYHTLGVHKYEAMEQEYKLKDVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3355..3798)
                     /locus_tag="ctg265_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRADRDFTAKDVDTAHRFRGFSCWGYHYYIRK
                     SGQIEPMRDEDTVGAHARGFNAISLGVCYEGGLDENGKAADTRTSRQKEAMHRLVNELL
                     QRYPDAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVKAETW"
     CDS             complement(3785..3952)
                     /locus_tag="ctg265_4"
                     /transl_table=11
                     /translation="MKEIVTKILDVIMFLVPFFGKRKRNRIVREVRFNATHKEVCNVKT
                     TEREKDDEKD"
     CDS             complement(3973..4476)
                     /locus_tag="ctg265_5"
                     /transl_table=11
                     /translation="MAMTVTYSVVPRKNPAKKDESAKYYAQAQASGELDFEELCEGITS
                     RSTCTETDVRAAISGILYEAKRALKAGRIVRLGDLGSLQIGLNSEGAVSGKEFSSSLIT
                     AAHIIFRPGKTLADITKILSYQQVTTRAVAQTGGSGNEGEDDDKGSGGGSDGGGSGEAP
                     DPAA"
     CDS             4670..4900
                     /locus_tag="ctg265_6"
                     /transl_table=11
                     /translation="MYYPDQPFHIRTYKKSELAHLYNPNVCLKVALQILRRWIVYNLPL
                     LQELEQEGYRARNRLLSPRQVATIIRYLGEP"
     CDS             4988..7105
                     /locus_tag="ctg265_7"
                     /transl_table=11
                     /translation="MDHFTIFQDFYRVIAEMTEEEIVSAISNGTYRETVEDVRRIFAEQ
                     GEKAANEKKIELPEITFSANYRGGRSNATLVKYLGYIVVDIDHQTQEALARILALAKKC
                     AHTRIAFISPKGMGLKIVVRVCRTDGTLPETIQEIEDFHHAAYHKIASFYAQLCGVEVD
                     TSGQDVSRTCLFSYDPDIYFNPDATAVIVEQPPMFFKSQKKKRASGKKKKTTAPDNNPL
                     TEQVALNYHSSHASLMVTLNYYHNKSEKYVTGNRNNYLHCLACMYNRYGVPQEEAAAFI
                     KSQFTDLPEDEMDALIGSAYGHNEEFDTRKLNSTQKRMLQIEQHIKENYDTRYNEVLHI
                     MEYRRRKTDTEQPEPFHILDEMMENSIWMEMNELGYSCTVKTIQNLIYSDFSITYHPIR
                     EYLDSLPEWDGTDYIGILANSVHTSHQKFWVECLERYLVGMCAAATQDDVVNHTVLLLC
                     SEIQNIGKTTFINNLLPPELRAYLSTGLINPNNKDDLAKIAQAMLINLDEFEGMNGREL
                     NIFKDLVTRKVISIRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRFLCFHVNSME
                     FIKINYTQLYAQIKYLLNKPGYQYWFTQSENSRIEENNEDFIFHSPEEELVLTHIRKPE
                     RFEKVHYLTVTEIAELIRERTGYQYSHGTKAQLGKVMSKHGFEFHKGKNGRRYTVFIID
                     TEQVKSNRLYE"
ORIGIN
        1 cagaaagcag caaaaagctg ctttctagcg gagagacagg gattcgaacc ccgggtacct
       61 cgcagtacaa cggttttcaa gaccgccgca atcgaccact ctgccacctc tccaaaactt
      121 ctttatcaga agtgcttttc gttaaaagcg gtgcaaaggt acgaatatat tttaaactta
      181 caagagtttt cacaaaaaaa aatcacgaac atcatttcca aagaatagca atatctaggt
      241 attttatgtt gaaaagcata aaatacagca tatttatgct gattatatga aaaaacgttg
      301 taactttgtg cgctctaaag aaaaggataa ctacaacctc taattttatc atttatggaa
      361 ttaaataaga tctttaaaga cggtctttgg agcacagaaa tcaacgtaag agatttcgtt
      421 agtcacaaca tcactccgta ttatggagat gcttctttcc tcgaaggacc taccgaacgc
      481 actaaagctg tatggaaccg ctgcctcgaa gcactggcag aagaaagagc caataacgga
      541 gtacgctcac tggataatgt caccgtctca accatcactt ctcataaagc cggatatatc
      601 gacaaagaaa acgaactgat cgtcggtctg caaacagacg aacttctgaa acgtgcaatc
      661 aaacctttcg gaggtatcaa cgtggtgagc aaagcctgcc acgaaaacgg cgtggaagtg
      721 gacgaccgtg taaaagatat attcactcac taccgcaaga cgcacaatga cggagtattt
      781 gacgtatata ccgaagaaat ccgttcgttc cgttctctgg gattcctcac cggacttcct
      841 gacaactatg cccgcggacg tatcatcggc gactaccgcc gtatggctct ctatggtatc
      901 gaccgactga ttgaggcaaa aaaagaagac ttgcgtaacc ttaccggtcc gatgaccgat
      961 gcccgcatcc gcctgcgcga agaagtggca gagcaaatca aggcattgaa ggacatgaag
     1021 gtaatgggcg aatactacgg actcgacttg agccgtcctg cctacaccgc acaagaagca
     1081 gtacaatggg tatacatggc ttaccttgct gccgtaaaag aacaagacgg tgctgccatg
     1141 tcactgggta acgtctcttc gttcctcgac atttatatgg aatatgaatt gagcaaagga
     1201 accatcacag aatctttcgc acaggagctg atcgaccagt ttgtcatcaa actgcgtatg
     1261 gtgcgccacc tgcgtatgca atcttacaac gacatctttg ccggcgaccc gacatgggta
     1321 accgaatctc tgggcggacg cctcaacgac ggacgcacga aggtaacaaa gacttctttc
     1381 cgcttcctgc aaacactcta caacctcggc ccttcaccgg aaccgaacct gactgtattg
     1441 tggagcccgg aactgccgga aggattcaaa gaattctgcg caaaagtatc tatcgacact
     1501 tcttccatcc aatatgaaaa cgacgacctg atgcgtgaag tacgccagtc tgacgactac
     1561 ggaatcgcgt gctgcgtatc ttatcaggaa atcggaaaac aaatccagtt cttcggcgca
     1621 cgttgcaacc tggctaaagc cctgctgctt gccatcaacg gcggacgttg cgaaaacacc
     1681 ggcacggtaa tggtgaaaaa catccccgta ctaaccagcg acacactgaa cttcgaagaa
     1741 gtaatgagca actacaagaa ggtattgaca gaaatcgccc gtgtttacaa cgaagcgatg
     1801 aacatcatcc attatatgca tgataagtat tactacgaaa aagcccaaat ggctcttgta
     1861 gataccaacc cgcgcatcaa ccttgcttac ggtgtagccg gactttctat cgccctcgat
     1921 tcactgtcgg ccatcaaata tgccaaagtg actgcacgcc gcaatgacat cggtctgaca
     1981 gaaggtttcg atattcaagg cgaattccct tgcttcggta atgacaacga caaggtagac
     2041 cacctgggtg ttgacctggt ctatttcttc agcgaagaat tgaagaaact tccggtttac
     2101 aaaaatgccc gtcctactct gtctttgctc accatcactt ccaacgtgat gtatggcaag
     2161 aaaacaggtg ctactcccga cggacgcgcc aaaggtgtcg cttttgctcc gggagccaac
     2221 cctatgcacg gacgtgacaa gaacggtgcc atcgcctctt tgagttccgt agcgaaatta
     2281 cgttaccgcg actcacagga cggtatcagc aacactttct ccatcgtccc caaatcactg
     2341 ggagctactg aagaggatcg cgtagaaaat ctggtgacaa tgatggatgg ctatttcacc
     2401 aaaggcgctc accacctgaa cgtgaacgta ctgaaccgtg aaatgcttta cgacgccatg
     2461 gaacatccgg aaaaatatcc gcaactcacc atccgtgttt ccggttatgc cgtaaacttc
     2521 gtgaagttga gccgcgaaca tcaattggaa gttatcagcc gtagcttcca cgaacgtatg
     2581 taatcagatc tactcaatat gacgataaac gtacattcat acgaaagtat gggaacattc
     2641 gacgggccgg gcttacggct cgtcgttttt cttcaaggat gcaatttccg ctgcctgtat
     2701 tgtgccaacc ccgacacgat agccggaaaa ggaggcacac ctaccccacc ggaagaaatc
     2761 gtccgcatgg ctatgagcca acgtcctttt ttcgggaaac gcggaggagt cactttctcc
     2821 ggaggagaac ctacgtttca ggccaaagca ctagtccctt tagtgcgtga actgaaagaa
     2881 aagggaatac atgtatgcat agacagcaac ggcggtatct ggaacgaaga agtagaagaa
     2941 cttttcaaac tgacggatct tgtattgctg gatattaaag agttcaatcc ggcacgccat
     3001 caagcactga ccggaagaag taacgagcaa accatccgca cggcagcatg gctggaagaa
     3061 aacgagaaac ctttctggct gcgttatgta ttagtgcccg gatacagcga tttcgaagag
     3121 gatatccgcc gcttgggaga ggctctggga aaatataaga tggtgcaacg agtggaaatc
     3181 ctgccttatc atacattagg cgtacataag tatgaagcga tggagcagga atacaagctg
     3241 aaagacgtaa aagaaaatac ccccgaacaa cttgaaaaag ccgcggaggt atttaaagag
     3301 tatttcacta ctgtggtggt caattaacca ccacagtaat aaataggata aaaatcacca
     3361 tgtctctgct ttcacctcga aacagggaca ctctttgatc cgctcccaag ggtcaacaat
     3421 tccattataa ttggtgtcag gactcaagtc ccgatgaccg accacctttg catccggata
     3481 acgctgcaac aactcgttta ccagacgatg catcgcctct ttctggcggg aagtacgcgt
     3541 atctgctgcc tttccgttct catccagccc gccttcatag cacactccta aactaatcgc
     3601 attaaaaccg cgtgcatgag cacccaccgt atcttcatcc ctcatcggct ctatctgtcc
     3661 cgacttgcga atgtagtaat gataccccca acacgaaaag ccccgaaagc gatgggcagt
     3721 gtccacatcc ttcgccgtga agtcgcgatc agcacgggta gcagagcagt gaatcacaat
     3781 cagactaatc tttctcatcg tccttctccc tttccgtcgt tttcacatta cacacttcct
     3841 tatgggtagc attgaagcgc acctctctca caattctgtt ccgctttctt tttccgaaga
     3901 aaggcacaag gaacataatt acatccaaaa tcttagttac aatttctttc atttgattca
     3961 atattaaagt tatcatgctg ccggatccgg agcttctccg cttccaccac cgtctgaacc
     4021 accaccggaa cctttgtcat cgtcttctcc ttcattgccg ctaccgcccg tttgggccac
     4081 cgcacgcgtc gttacctgct ggtagctaag aatcttcgtg atgtccgcca acgtctttcc
     4141 gggacgaaaa ataatatggg cagccgtaat cagcgaactt gaaaactcct tgcccgacac
     4201 agctccttca ctgttcaacc cgatctgcaa actacccaaa tcacccagcc ggactattct
     4261 tcccgctttc aacgcacgct tcgcctcata aagaataccc gaaatggcgg cacgcacatc
     4321 tgtctccgta caggtagacc ggctggtgat tccctcgcac aactcttcaa aatccaactc
     4381 tcccgaagcc tgtgcctgcg cataatactt tgccgactca tcctttttgg caggattctt
     4441 gcgcggcact actgaataag ttactgtcat tgccataata aaataatttt aagatttcac
     4501 acatttcgaa cgcgttcctt atttgttaac gacataaaga taacacattt tccatcctcc
     4561 ggattcccca acgaagtttc ctgatactct atgaataata atgacgttat tcttttaata
     4621 aaccattaat tttcagtaac tttatagcat aaaccaaaca tcattcatca tgtattatcc
     4681 ggatcagccg tttcatatcc gcacttataa aaagtcggaa ctagctcatc tttacaaccc
     4741 caacgtatgc cttaaagtag ccttacaaat actacgtcgt tggattgtct acaaccttcc
     4801 gctcctgcaa gaactcgaac aagaaggcta tcgtgcccgc aaccgccttc tctctccccg
     4861 gcaagtggca acgatcatac gctatttggg agaaccctag gaacttaatc aaaaaaacga
     4921 aaagttccat tctattattt tacaaacaaa tttctttgca accgcaaatt aaaaagaaca
     4981 aaccaacatg gatcatttta ccatctttca agacttttac cgcgtcatag ccgaaatgac
     5041 agaagaagaa atcgtatcgg ccatcagcaa cggcacatac cgggaaacag tggaggacgt
     5101 gcgccgaatc ttcgcggaac aaggcgagaa agcagccaac gaaaagaaaa tagaactacc
     5161 ggaaatcacc ttttcagcca actatcgggg aggacgttca aacgcaactc tcgtcaagta
     5221 tctgggatac atcgtggtag acatcgacca tcagacacag gaagcattgg cacgcatcct
     5281 cgcactggca aaaaagtgtg cccacacccg catcgctttc atcagcccca aaggaatggg
     5341 actgaaaatc gtggtacgtg tatgccggac agacgggaca ttgcccgaaa caattcagga
     5401 aattgaagat ttccatcatg ctgcttatca caaaatcgct tccttttatg cccagctctg
     5461 cggagttgag gtcgacactt ccgggcagga tgttagccgc acttgcctgt tctcttacga
     5521 tccggatatt tatttcaatc ccgatgccac agctgtcatc gtggaacagc caccgatgtt
     5581 cttcaagtct caaaagaaga aaagggcatc cggcaaaaag aaaaagacga cagcaccaga
     5641 caacaatccc ctcaccgaac aagtggcact caactaccat tcctcgcacg cttccttaat
     5701 ggtgactctg aactattatc acaataaatc cgaaaagtac gtcaccggca accggaacaa
     5761 ctacctgcat tgcctggctt gtatgtacaa tcgctacggc gtcccccagg aagaggcggc
     5821 agcttttatc aagagccagt tcaccgatct tcccgaggat gaaatggacg cactaatcgg
     5881 gagtgcttac ggacacaacg aggaattcga cacccgcaag ctgaatagta cgcagaagcg
     5941 aatgttacag attgagcaac atatcaaaga aaattacgat acacgctaca acgaagtgct
     6001 tcatatcatg gaatatcgca gaaggaaaac ggacacggag caaccggaac ctttccatat
     6061 tctcgacgaa atgatggaaa acagcatttg gatggagatg aacgaactgg gctattcgtg
     6121 caccgtcaag acgattcaga atctgatcta ctccgacttc agcatcacct accatcctat
     6181 tcgcgagtat ttagactcgc tgcctgaatg ggacggaacg gattatatcg gtatccttgc
     6241 caacagcgtg catactagtc accagaaatt ttgggtagaa tgtctggaac gttatctggt
     6301 gggaatgtgc gctgccgcca cacaagacga tgtggtgaac cacactgttt tgttgctttg
     6361 cagcgaaata cagaacatcg gaaagactac gtttatcaac aacctgctgc cgcccgaatt
     6421 gcgcgcctat ctttctaccg gtcttatcaa cccgaacaac aaggatgatc tggccaaaat
     6481 agcgcaggca atgcttatca atctggatga gtttgaaggg atgaacggac gggaactgaa
     6541 tatcttcaag gatttggtta cccgcaaagt aatcagcatc cgcctgccat acgcgcggcg
     6601 ttcgcagaac tttcctcaca cggcttcttt tgccggcaca tgcaattatc aggaagtgct
     6661 tcacgacacc actggtaacc gccgttttct ctgctttcat gtcaactcga tggagtttat
     6721 caaaataaac tacacgcaat tatatgcaca aatcaagtat ctactcaaca agccggggta
     6781 tcagtattgg ttcacccaat cggaaaactc acggattgaa gagaacaacg aggatttcat
     6841 tttccactca ccggaagaag aactggtgtt gacacatatc cgcaaaccgg aacgtttcga
     6901 aaaggtgcat tatctgacgg tcactgaaat tgcggaattg attcgcgaac gaacggggta
     6961 tcaatactct cacggaacca aagcccaact aggaaaagtg atgtccaaac acggcttcga
     7021 atttcataag ggaaaaaacg ggagaagata cactgtattc attattgata cggaacaggt
     7081 gaaatcaaac aggctatatg aataagattt catctccttc gcacgcaaaa aggtgagggt
     7141 tggtgagggt acttttgcaa cttttcattt ttatatttct tatttctatt cttctatttt
     7201 cttctaaaaa aaaaaatacg aatgttagaa atttaccctc acctaccctc acc
//
