LOCUS       gnlProkkaCACIEOLF_1403  4627 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CACIEOLF_1403.
ACCESSION   gnlProkkaCACIEOLF_1403
VERSION     gnlProkkaCACIEOLF_1403
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-17 10:53:53
            Original ID  :: gnl|Prokka|CACIEOLF_1403
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4627
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4627
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3412:4627]"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3413..4627
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(APE_KS1 or APE_KS2)"
                     /neighbourhood="20000"
                     /product="arylpolyene"
                     /protocluster_number="1"
     cand_cluster    1..4627
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(APE_KS1 or APE_KS2)"
                     /kind="single"
                     /product="arylpolyene"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..4627
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="arylpolyene"
                     /region_number="1"
                     /rules="(APE_KS1 or APE_KS2)"
                     /tool="antismash"
     CDS             complement(201..407)
                     /locus_tag="ctg1403_1"
                     /transl_table=11
                     /translation="MILFSETGEKLVNNRLNWSFGTFILTSDMWKQSEGWKGTFKYGLF
                     LTYYLVEYVHISVEFLHRIVLET"
     CDS             494..925
                     /locus_tag="ctg1403_2"
                     /transl_table=11
                     /translation="MEEIVFHHTLPIQLRFNDVDKFGHVNNTVYFSFYDLGKTEYFGSV
                     CPGVDWEKIGIVVVHIEANFVKQIFASDHIAVQTAVSKIGTKSFHLVQQVIDTKTNEVK
                     CVCKSIMVTFNLERHESMPLTKEWIEAICKYEGRDLQKA"
     CDS             1133..2656
                     /locus_tag="ctg1403_3"
                     /transl_table=11
                     /translation="MIADKSINLDTLHKVLFDNEKLELSEECIRKVEESFDFLQSFSSD
                     KIIYGINTGFGPMAQYRIEDQSLIDLQYNIIRSHSTGAGKPLPELYVKAAMIARLYTFL
                     QGKSGVHLELVSLLCEFINRGIYPFIPEHGSVGASGDLVQLAHIALTLIGEGEVFYQGK
                     LCNAATVLQENGLKPFSMRIREGLSVTNGTSVMTGIGIVNLIYAKKLLRWSVAASVMMN
                     EIAASYDDFMAQSLNEAKHHKGQQEIAAMMREWVAGSKCVLQRENELYNQVHKEKIFEH
                     KVQPYYSLRCVPQILGPIYDELENAEEVLINEINSACDNPIVDPDTQNIYHGGNFHGDY
                     ISFEMDKLKIAVTKLTMLCERQINYLFHDRINGILPPFVNLGVLGLNYGLQASQFTATS
                     TTAECQTLSNPMYVHSIPNNNDNQDIVSMGTNSALLAKTVIENSYQVMAIQFMGMAQAI
                     DYLKIQDRLSSKSRQVYEEIRSFFPVFTNDTPKYKEIEMMIDYLKKEDK"
     CDS             2658..3386
                     /NRPS_PKS="Domain: PKS_KR (3-169). E-value: 2.3e-16. Score:
                     52.1. Matches aSDomain: nrpspksdomains_ctg1403_4_PKS_KR.1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     215.6; E-value: 1.3e-65)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg1403_4"
                     /sec_met_domain="adh_short (E-value: 9.4e-36, bitscore:
                     111.9, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYALVTGGSRGIGRAVSCKLAEMGYFILINYQNNDAEAEKTLQL
                     VQEKGSNGELMKFDVTDPAAITLALGNWASQHPDEYIEVLINNAGIRKDNLMLWMTGEE
                     WSKVLDISLNGFFNVTQPLLKNMLVKRYGRIVNIVSLSGIQGMPGQANYSAAKGGVIAA
                     TKALAQEVAKKKVTVNAVAPGFIRTDMTEGIDENEWKKHIPAGRFGTPEEVADLVGFLA
                     SPASSYITGEVISINGGLYT"
     aSDomain        2667..3164
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1403_4_PKS_KR.1"
                     /evalue="2.30E-16"
                     /label="ctg1403_4_PKS_KR.1"
                     /locus_tag="ctg1403_4"
                     /protein_end="169"
                     /protein_start="3"
                     /score="52.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="ALVTGGSRGIGRAVSCKLAEMGYFILINYQNNDAEAEKTLQLVQE
                     KGSNGELMKFDVTDPAAITLALGNWASQHPDEYIEVLINNAGIRKDNLMLWMTGEEWSK
                     VLDISLNGFFNVTQPLLKNMLVKRYGRIVNIVSLSGIQGMPGQANYSAAKGGVIAATKA
                     LAQ"
     CDS_motif       2670..2732
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ctg1403_4_0001"
                     /evalue="2.90E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="ctg1403_4"
                     /protein_end="25"
                     /protein_start="4"
                     /score="13.5"
                     /tool="antismash"
                     /translation="LVTGGSRGIGRAVSCKLAEMG"
     CDS             3413..4627
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (65-404). E-value:
                     3.2e-53. Score: 173.0. Matches aSDomain:
                     nrpspksdomains_ctg1403_5_PKS_KS.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     arylpolyene: APE_KS1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 295.7; E-value:
                     9.5e-90)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1403_5"
                     /sec_met_domain="APE_KS1 (E-value: 6.7e-160, bitscore:
                     520.6, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKRVVITGMGIYSCIGKNLDEVKDSLYNGKSGIGIDPVRKELGYF
                     SALTGILERPDLKKLLDRRKRLCLPEQGEYAYLATLEAFRNAGINEDFLEANEVGILYG
                     NDSSAAPVINAVDIIREKKNTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTIAGACA
                     SGSHAIGMGYLLIKSGLQDCILCGGAQEVNPYAVGSFDGLSAFSTQEAAPEKASKPFDK
                     RRDGLIPSGGAASLVLESYESAVKRGAPILAEVIGYGFSSNGDHISVPNVDGPKRSLQM
                     AIKDAGIALEQINYINAHATSTPVGDLNEAKAIAEVFEGHHPYVTSTKSMTGHEMWMAG
                     ASEVIYSTLMMNNGFIAPNLNFEEPDEASAQLNIPTQRVNLEFDTFLSNSFGFGGTNST
                     LIIRKI"
     aSDomain        3608..4624
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg1403_5_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="3.20E-53"
                     /label="ctg1403_5_PKS_KS.1"
                     /locus_tag="ctg1403_5"
                     /protein_end="404"
                     /protein_start="65"
                     /score="173.0"
                     /tool="antismash"
                     /translation="RLCLPEQGEYAYLATLEAFRNAGINEDFLEANEVGILYGNDSSAA
                     PVINAVDIIREKKNTALVGSGSIFQSMNSTVTMNLSVIFKLRGVNFTIAGACASGSHAI
                     GMGYLLIKSGLQDCILCGGAQEVNPYAVGSFDGLSAFSTQEAAPEKASKPFDKRRDGLI
                     PSGGAASLVLESYESAVKRGAPILAEVIGYGFSSNGDHISVPNVDGPKRSLQMAIKDAG
                     IALEQINYINAHATSTPVGDLNEAKAIAEVFEGHHPYVTSTKSMTGHEMWMAGASEVIY
                     STLMMNNGFIAPNLNFEEPDEASAQLNIPTQRVNLEFDTFLSNSFGFGGTNSTLIIRK"
ORIGIN
        1 tcattattac ttccattatt gttctaaaat tagaatagtt attaggatag attaccatca
       61 atattgggca atttatgtgc caaaagcata acaaaagact gatacaacga gacttatcaa
      121 gtttaacaaa agagattata tcactaattt tcaaatacat aaacaatttg caataaagaa
      181 aaaaagcatt aataaccaga ttaagtttca agcacgatcc tgtgcaaaaa ttccacagaa
      241 atgtggacat attccacaag ataataagtt aagaataatc cgtatttaaa agttcctttc
      301 catccttctg attgtttcca catatcagaa gtcagaataa acgttccaaa actccagttt
      361 aaacgattat taactaattt ctcccccgtc tccgagaaca gaatcatcag aaatatctat
      421 ctttgcccca atttcaagca agaaagcaaa agtgctattt ttgtgtcgaa tttaaaacgt
      481 ttaagagaaa acaatggaag aaatcgtatt tcatcacaca ttacctatcc aattgcgatt
      541 caatgatgta gacaaatttg gtcacgtcaa caacacagtt tacttctcct tttacgactt
      601 gggaaagact gaatacttcg gttcggtatg tccgggagta gattgggaaa agattggcat
      661 tgtcgttgtt catattgaag cgaatttcgt taagcaaatt ttcgcatcgg accacatcgc
      721 tgtacaaacc gccgtctcta agataggtac caaaagcttt catctggttc aacaagtcat
      781 tgacacgaaa accaatgagg tgaaatgtgt ttgcaagtcc atcatggtta ctttcaatct
      841 tgaaagacat gagtccatgc cactaaccaa ggaatggata gaagcgatct gcaagtatga
      901 aggacgggat ttgcaaaaag catagattga ttccccccca ccgatgaaga caccctccgg
      961 caccaatgaa agcatcgagc ggtaccgggg aacaaatctc ccacgaataa gaaacaaaac
     1021 aagcaaaacc aattccttag aaaaagtttt atcctgattc atgtccgcta tataacagaa
     1081 attgcctatt tttgcacctt attattaagg cataaaatat ctagtacaga aaatgatagc
     1141 tgacaaaagt ataaatttag atacccttca taaagtattg tttgataatg agaagctgga
     1201 actctctgaa gaatgtattc gaaaagtaga ggaaagcttc gattttctgc aatccttttc
     1261 cagcgataag attatttatg gtatcaatac ggggttcggc ccaatggcac aatacagaat
     1321 agaagatcag tcattgatcg accttcagta taatatcatc cgaagccatt ccaccggtgc
     1381 cggcaaaccg cttcccgaac tttatgtaaa agcagctatg attgcccgtt tgtacacttt
     1441 tctacaaggg aagtcaggag tgcatctgga actggtttct ctcctctgtg aatttatcaa
     1501 ccgcggaatt tatccgttca tacccgaaca cggaagtgta ggtgccagcg gcgatctcgt
     1561 acaactggcc catatcgccc tgacgttaat aggggaaggg gaagtttttt atcagggtaa
     1621 attgtgtaac gcagctacgg tgcttcagga aaacggcctg aaaccttttt ccatgcgtat
     1681 tcgtgaaggt ttatccgtta caaacggtac ttctgtaatg acaggcatcg gtattgtcaa
     1741 tctgatttat gcaaaaaaac tactccgttg gtcggtggct gcctctgtaa tgatgaatga
     1801 gattgccgcc tcttatgatg attttatggc acagtcatta aacgaggcca agcatcataa
     1861 aggtcaacaa gagatagctg ctatgatgag agaatgggtg gcaggcagta aatgcgtgct
     1921 tcaaagagag aacgagctat acaaccaggt gcataaagag aaaatcttcg aacacaaagt
     1981 acagccctat tattccttgc gatgtgttcc gcaaatactc ggtcctattt acgatgaact
     2041 ggagaatgcg gaagaagtat taataaacga aataaattcc gcctgtgaca atccgattgt
     2101 cgatccggat acacaaaata tttatcatgg cggcaacttc cacggagatt acatttcttt
     2161 cgaaatggac aagttgaaaa ttgccgtgac caagctgact atgctttgcg aaagacagat
     2221 taactatctg ttccacgacc gtatcaatgg catcctgcct ccgtttgtaa atttgggagt
     2281 gcttggattg aactatggtt tacaggcttc acaattcact gcaacctcca ccacagcgga
     2341 atgtcagaca ttatcaaatc cgatgtatgt acacagtatc cccaacaaca atgataatca
     2401 ggatattgtc agcatgggaa ccaactcggc tctattagca aaaacagtca ttgagaattc
     2461 ttatcaggtg atggctatcc agtttatggg aatggcacaa gctatcgact acctgaaaat
     2521 acaggatcgc ctaagttcca aaagcaggca ggtttatgaa gaaatacgca gtttcttccc
     2581 tgtatttacc aatgacacac ctaaatataa agagatagaa atgatgatag actatctcaa
     2641 aaaagaagat aaataaaatg aaatatgcat tagtaaccgg aggaagccga ggtatcggcc
     2701 gtgctgtcag ttgtaaactg gcggaaatgg gctactttat actgataaat tatcagaata
     2761 atgacgcaga agcggagaaa acgttgcagt tagtacagga aaaaggaagt aatggcgagt
     2821 tgatgaagtt cgatgtgacc gaccctgccg ctatcaccct tgctttgggc aactgggctt
     2881 cccaacaccc tgacgaatat atcgaggtac tgataaacaa tgcaggtatc cgcaaagaca
     2941 acctgatgct ctggatgaca ggagaagaat ggagcaaagt gctcgatatc agcctgaacg
     3001 gattcttcaa cgtgacacag cctttattga agaatatgct ggtcaaacgt tatggacgta
     3061 tcgtaaatat cgtgtcattg tcgggcattc agggaatgcc cggccaagcc aattactccg
     3121 cagccaaagg tggcgtaata gctgctacca aagcactggc acaggaagtt gccaagaaga
     3181 aagtaacagt caatgcagta gctccgggat tcatccgtac ggatatgacg gaaggaattg
     3241 atgagaacga atggaagaaa catattcccg cgggacgttt cggcactccg gaagaggttg
     3301 ccgacctggt cggtttcctg gcatctccag cctcatccta catcacagga gaagtgattt
     3361 ccatcaacgg aggactatat acttaaaata aaagcataga atagacaaaa taatgaaaag
     3421 agttgttatc acgggaatgg ggatttactc ttgcatcggc aagaatctgg atgaagtaaa
     3481 agactcacta tataatggga aatcgggaat tggcatagac ccagtacgga aagaattggg
     3541 ttatttttct gcattaacgg ggatattgga acgtcccgac ttgaagaagt tgttggaccg
     3601 ccgtaaacgt ctctgtctac cggagcaggg agaatatgct tatctcgcta cattagaagc
     3661 tttccgtaat gccggtatta atgaggattt tctcgaagcc aatgaagtgg gtattttgta
     3721 tggcaatgat agtagtgccg ctcctgttat taatgcagtg gacatcattc gtgaaaagaa
     3781 aaacactgca ctggtaggtt ccggttctat tttccagtcg atgaattcca ccgtgacaat
     3841 gaacctgtca gttattttca aactaagagg ggtcaacttc accattgccg gagcctgtgc
     3901 aagcggttct cacgctattg gtatgggtta cctgcttata aaatcgggat tacaggattg
     3961 tattctttgc ggtggcgcac aagaagtcaa tccttatgca gtaggaagtt tcgacggact
     4021 aagcgcattt tccacccagg aagcagcccc cgagaaagcc tcgaaacctt ttgataaacg
     4081 acgtgacgga ctgattccga gtggaggagc cgccagtctg gtattagaga gttatgaatc
     4141 ggctgtaaaa agaggagctc ccattttggc cgaagtcatc ggttacggtt tttcatccaa
     4201 tggagatcac atctccgtgc ctaatgtgga cggtccgaaa cgttctcttc aaatggctat
     4261 caaagatgca gggattgcac tcgaacagat caattatatc aatgcacacg ccacttcaac
     4321 tccggtaggt gacttgaacg aagcgaaagc cattgcggaa gtatttgaag ggcatcaccc
     4381 gtatgtgacc tccacaaagt ctatgacagg acatgaaatg tggatggccg gtgccagtga
     4441 agtgatctat tccaccctca tgatgaacaa cggtttcatc gctcccaacc tcaatttcga
     4501 ggaaccggac gaggcctctg cacaattaaa tatacctacc caacgggtaa acctggaatt
     4561 tgatacgttc ctttccaact cattcggatt cggaggaacc aattccacgc taatcatacg
     4621 taaaatc
//
