LOCUS       c00467_gnlPro..         3239 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PFMILDCK_468.
ACCESSION   c00467_gnlPro..
VERSION     c00467_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 09:11:46
            Original ID  :: gnl|Prokka|PFMILDCK_468
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3239
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3239
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[150:3237]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      151..3237
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3239
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3239
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..61)
                     /locus_tag="ctg467_1"
                     /transl_table=11
                     /translation="MLNSIKIPIYGEKPDSSYYF"
     CDS             151..2379
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg467_2"
                     /sec_met_domain="Gly_radical (E-value: 1.1e-47, bitscore:
                     148.8, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1167.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFKDGLWSKEINVRDFVSNNITPYEGDASFLQGPTERTKA
                     VWNHCLKALEEERNNNGIRALDYTTVSTITSHPAGYIDKENELIVGLQTDQVLKRAIKP
                     FGGINVVMKACRENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGLDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKEIK
                     VLGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSQ
                     GTITETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKAFCAQVSIDTSSVQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKDIPVLTGETL
                     KFEEVMANYKKVLIQIARVYNEAMNIIHYMHDKYYYEKAQMAFVDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAHVKARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLN
                     REMLQDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2512..3237
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.2;
                     E-value: 1.7e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg467_3"
                     /sec_met_domain="Radical_SAM (E-value: 2.6e-30, bitscore:
                     93.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTPAGEILQMAVSQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYQLKEVVENTPA
                     QLERAEKLFKEYFPTVVVN"
ORIGIN
        1 ggaagtaata actgctatct ggcttctccc cataaatagg tatttttatg ctattcaaca
       61 taaaatagga cccttttata tacctaaacc gaaaaaaggt tgtaaatttg cgcgctctaa
      121 aggataacta accactaact gattaatttt atggaactga ataagacctt taaagatgga
      181 ctttggagca aagaaatcaa tgtaagggat ttcgttagta acaacatcac tccttatgaa
      241 ggggatgctt ctttcctgca aggacctacc gaacgcacca aagctgtctg gaatcactgt
      301 ctcaaagctc tcgaagaaga gagaaacaat aatggtattc gtgcactcga ctacacaact
      361 gtatctacta taacctctca ccctgccggc tacatcgata aagaaaacga actgatcgtc
      421 ggactacaga ccgatcaggt gttaaaacgt gccatcaaac cgtttggcgg aatcaatgtg
      481 gttatgaaag cttgccgtga aaacggagtg gaagtagatg acagagttaa ggatatcttc
      541 actcactatc gcaagactca caatgatggg gtattcgatg tgtataccga agaaattcgt
      601 tcgttccgtt cattgggatt ccttaccgga cttcccgaca actatgcacg cggacgtatc
      661 atcggtgatt atcgccgcct ggctctctat ggtcttgacc gtctgataga agctaaaaaa
      721 gaagatctgc ataatctgac aggtcctatg actgaagccc gcattcgtct tcgtgaagaa
      781 gtagccgagc aaatcaaggc cctgaaagag attaaagtat tgggcgaata ttatggtttg
      841 gatctgagcc gaccggcata taccgcacag gaagctgtac aatgggtgta tatggcttat
      901 ctggctgccg taaaagagca ggatggtgct gccatgtcac tgggtaatgt atcttcattc
      961 ctcgacatct atcttgaata tgaattaagc cagggtacga taaccgaaac attcgctcag
     1021 gaactgatcg accaattcgt tatcaaactc cgtatggtac gccacttgcg tatgcagtcg
     1081 tacaacgata tttttgcggg tgatcccaca tgggtgaccg aatctatcgg tggacgtttc
     1141 aatgacggac gtaccaaagt tactaaaact tctttccgct tcttgcagac actttataac
     1201 ttaggcccgt caccggaacc taacatgact gtactatgga gccctgagtt acccgaaggc
     1261 ttcaaagcat tctgtgcaca agtatctatc gacacttctt ccgtacaata cgaaaacgac
     1321 gacctgatgc gtgaagtccg tcaatcggat gactacggaa tcgcttgttg tgtttcatat
     1381 caggaaatcg gaaagcagat tcagttcttc ggcgcacgtg ccaaccttgc caaagccctg
     1441 ctactggcta tcaacggagg acgttgcgaa aatacaggta cagttatggt aaaagacatc
     1501 cctgtattga ccggtgaaac actgaagttc gaagaagtaa tggccaacta taagaaagta
     1561 ttgatacaaa ttgcacgtgt atacaacgaa gcaatgaata tcatacatta catgcacgat
     1621 aagtattact acgaaaaggc gcagatggct ttcgtggata ctgatccccg cattaacctc
     1681 gcttatggtg tagccggact ttcaatcgcc atcgattcac tgtccgccat caaatatgca
     1741 catgtgaaag cccgtcgcaa cgacatcgga ctgacggagg gattcgatat cgaaggtgct
     1801 ttcccttgtt tcggtaatga cgatgaccgt gtagaccacc tgggtgtgga cctggtatat
     1861 ttctttagcg aagaattaaa gaaactcccc gtatataaga atgcccgtcc taccctttcg
     1921 ctgctgacta tcacttcgaa cgtaatgtat ggcaagaaga ccggtgccac tcctgacgga
     1981 cgcgccaaag gcattgcctt tgctcccggt gccaacccca tgcacggacg tgataaaaat
     2041 ggtgcaatcg cctcactgag ttctgtagcc aagttgcgtt atcgcgactc acaggatggt
     2101 atcagcaata cgttctcgat cgttcccaaa tcattgggag caacacaaga agaccgtatc
     2161 gacaatctgg tgaccatgat ggacggatat tttacaaaag gcgcacacca cctgaacgta
     2221 aacgtgctga accgggaaat gttgcaggat gcgatggaac atcccgaaaa gtatccgcaa
     2281 ctgactatcc gtgtctcagg atatgccgtt aatttcgtga aattgagccg cgaacatcag
     2341 ttggaagtaa tcagccgcag tttccacgaa agaatgtaat cggtaaaccg gatacataac
     2401 cttttaaagc acggattaca cggatttcac ggattaagca aaaatccgga gtaacacccc
     2461 tgtagtccgt gctttctttt ttactacatt ctttgcttat aatacactat tatgattaat
     2521 gttcattcat acgaatcaat gggcactttc gatggtccgg ggcttcggct tgtcgtattc
     2581 cttcagggct gtcctttccg ttgcctgtat tgtgccaacc ccgatacaat agatgtaaag
     2641 ggcggtactc ctactcctgc cggtgaaatc ctgcaaatgg ctgtcagcca aaaagctttc
     2701 ttcggaaaaa aagggggaat tactttctcc ggcggtgagc ctaccctgca agcagaagcg
     2761 ctgattcctc ttttcaagga gctgaaggcg aacggcatcc atatctgtct ggacagtaac
     2821 ggaggcatct ggaatgaaaa ggtggaagag ctctttagcc tgactgacct cgtgctgctg
     2881 gacatcaagc aattcaatcc cgaacgccac cgtacgctga cgggacgaag caacgaacag
     2941 acactccgca ccgctgcctg gctcgaagaa catgaacacc ccttctggct gcgctatgta
     3001 ctggtccccg gttacagcga ttcggaagaa gacacccgcc tgctgggtga aagtcttggc
     3061 aaataccagc agatacaacg ggtggaaata cttccctacc accgcttagg ggtgcataaa
     3121 tacgaagcta tgggctggga ttatcagtta aaagaagtag tagagaatac ccccgcacaa
     3181 ctggaacgag ccgaaaaact ctttaaagag tactttccca cagtagtggt aaattgaga
//
