LOCUS       c00356_gnlPro..         7069 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CPAIFJOA_356.
ACCESSION   c00356_gnlPro..
VERSION     c00356_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 13:00:12
            Original ID  :: gnl|Prokka|CPAIFJOA_356
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7069
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7069
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[237:3263]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      238..3263
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7069
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7069
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             238..2466
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg356_1"
                     /sec_met_domain="Gly_radical (E-value: 4e-48, bitscore:
                     151.2, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEMNKVFKDGLWSKEINVSDFVHTNITPYEGDASFLAGPTERTKK
                     VWNECLKALEEERANNGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKP
                     YGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLHNITSPMTDARIRLREEVAEQIKALKEIK
                     ILGEYYGLDLGRPATNAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDH
                     GKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLL
                     NFEEVWSNYKKVLMQIARVYNDAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENAYPCFGNDDDRVDHLGVDLVYFFSEEL
                     KKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDAQDGISNTFSIVPKSLGVDQETRIENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQLTIRVSGYAVNFIKLSREHQLEVISRSFHERM"
     CDS             2535..3263
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 77.8;
                     E-value: 3.2e-24)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg356_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-28, bitscore:
                     89.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGE
                     STETTIDEIVRMAVSQKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQSIHICIDTN
                     GSIWNEEVKELLQWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAGWLEKNGKPFWLRY
                     VLVPGYSSFEEDIRALGEHFKNYQMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTL
                     KQLETAKTLFGEYFNTVYLN"
     CDS             3365..4114
                     /locus_tag="ctg356_3"
                     /transl_table=11
                     /translation="MKRLLLLCLTYIMCVGNHQMAAQDSSESPGIFNSLAVGVSASTTG
                     IGIDVASPIGSYLAVRAGVSFMPNFTYSTDVDVDIEGSYEGNIPTSIDVEGGMGRTAGE
                     ILLNVYPFKSSSFFVCGGAYFGGGKLIKIKGHSDELAELIKEGQEVGIEIGNYRIPVDQ
                     NGNISGGLKVSSFRPYLGLGFGRAIPKKRIGFMFEMGVQFHNTPKVYTNNGDLSDLTAE
                     ADNDFTDIIDKLTVYPVLKFRLCGKIF"
     CDS             complement(4193..4780)
                     /locus_tag="ctg356_4"
                     /transl_table=11
                     /translation="MSSREDILQSIRRATHVKYEKPDLKPLEDMALTYPSKVEQFYLIM
                     KQVGGEAILLEKEEDVNDFIKKAYPGAKRIASNLKTITCATFNPDDVEDPAELNGTDLA
                     VIDGKIGVAENGAVWIEQDVKQRAIYFIAEKLVILLNKNKIVNNMHEAYKLIDTGEYGF
                     GTFISGPSKTADIEQALVMGAHGARDVMVVLI"
     CDS             complement(4783..6150)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg356_5"
                     /sec_met_domain="Fer4_8 (E-value: 8.7e-13, bitscore: 37.4,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2.3e-06, bitscore: 16.8,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKHSKAAAEFLKNKKQAAWHDETLWLVRAKRDRLSKEVPEWEE
                     LRNMACATKLYSNSHLDELLVEFENNARANGAHVYWAKDADEYCNIVYNILNQHGVKHF
                     IKSKSMLAEECELNPFLENKGIEVVESDLGERILQLMHLKPSHIVLPAIHIKREQVGEL
                     FEREMGTEKGNFDPTYLTHAARKNLRQKFIHAEAAMTGANFAVASTGEIVVCTNEGNAD
                     MGTSQPKLQIAAFGMEKIVPDRESLGVFTRLLARSATGQPITTYTSHYRKPREGGELHI
                     IIVDNGRSKILADQKHIKTLNCIRCGACMNTCPVYRRSGGYSYTYFIPGPIGINLGMLK
                     APLHYYDNVSACSLCYSCSFVCPAKVDLAEQIYLWRQDLDKLGKADRMKRVMSGGMEFM
                     MNRPLIFNMALKWAPLVNGMPRFLIYNGLNDWGKGREMPKFAKESFNEMWKKGKVK"
     CDS             complement(6147..6893)
                     /locus_tag="ctg356_6"
                     /transl_table=11
                     /translation="MTMKVGLFIPCYINAVYPQVGIASYKLLKSLGVDVDYPLNQTCCG
                     QPMANAGFENKAVDLARHFDEQFKNYDYIVGPSASCVVFVRDNYSRLLKEDKHRCASEG
                     KIYDICEFIHDIVRPSSLKAKFPHKVSLQNSCHGVRLMKLSSASELNIPYFSKLRDLLS
                     LVEGVEVVEPERKDECCGFGGMFAVEEDAVSVQMGHDKVMRHIATGAEYIVGADSSCLM
                     HQNGIIARDKLPIKTLHIVEILAAGL"
ORIGIN
        1 gacggtaacc gttagtttcc ataatcgctg atgattcgtt tgtttcacac aaagatagga
       61 ttttatcgga tatacccctc aggattttac gttgacccct tattgttttt tcatttattg
      121 accactttat acttaaaaat gcacaagtat tcgcattata cctactaata gtgatgcata
      181 gctcacaaaa aaggagtacc tttgcagccc taaatctctt aataatggaa taacagtatg
      241 gaaatgaaca aagtttttaa ggatggattg tggagtaagg aaattaatgt ttcagacttc
      301 gtacatacca acattacccc ttatgagggc gatgcctcat ttctggcagg ccccacagag
      361 cgaactaaaa aagtgtggaa cgaatgcctc aaggcactag aagaagaacg tgccaataac
      421 ggagttcgtt cccttgataa tgtaacagta tctaccatca cctcacataa agcaggatac
      481 attgaccgtg aaaacgaact gattgtaggt cttcaaacgg atgaacttct gcgccgtgcc
      541 atcaaacctt atggtggtat caaagtcgta gaaaaagcct gccatgaaaa cggtgtggaa
      601 gtagatgaaa aagtaaagga tatctttact cattatcgca aaacccataa tgatggcgta
      661 tttgatgctt acaccgaaga aatccgctct ttccgttcac tcggtttcct gaccggtctg
      721 cccgataatt atgcacgcgg gcgtatcatc ggtgactacc gccgtttggc actgtatggt
      781 atcgaccgac tgattgaagc aaaacaagaa gatttgcaca atatcaccag tccgatgacc
      841 gatgcccgta tccgcctgcg cgaagaagta gccgaacaaa tcaaagcact gaaagaaata
      901 aagatattgg gtgaatatta cggactcgac ttgggtcgtc ccgctactaa tgcacaagaa
      961 gctgttcagt gggtttatat ggcatatctt gctgccgtga aagaacaaga tggagcagct
     1021 atgtcattgg gcaacgtttc ttcattcctg gatatctata ttcaatatga tttggatcat
     1081 ggtaaaattg acgagtcatt tgcgcaggaa ctggtagacc agttcattat caagttgcgt
     1141 atggtccgtc atctgcgtat gcaatcatac aacgatatct ttgcaggtga tcccacctgg
     1201 gtgaccgagt ctatcggcgg acgcttcaac gacggacgta ccaaggttac aaaaacttcc
     1261 ttccgtttct tgcagacact atacaatctg ggtccgtcac ccgaacctaa tatgactgta
     1321 ctgtggagtc cggaattgcc ggaaggtttc aaagaattct gtgcacaagt ttctatcgac
     1381 acttcttcta tacaatacga aaacgacacg ctgatgcgcg aagtgcgtaa ctgtgatgat
     1441 tatggtatcg cctgctgcgt ttcttatcag gctatcggca aacaaatcca gttcttcgga
     1501 gcacgttgca atctggctaa agccctattg ctggctatca atggcggacg ttgtgagaat
     1561 acaggaactg taatggtgaa agatattcct gtattgacag gggacctgct gaactttgaa
     1621 gaagtatggt ctaattataa gaaagtatta atgcaaattg cacgtgtgta taatgatgcg
     1681 atgaatatca ttcactatat gcacgataaa tattattatg aaaaggctca aatggcattc
     1741 attgataccg atccacgtat caatctggca tatggcgtag ccggtctgtc cattgccatt
     1801 gactcattgt ctgccatcaa atacggcaaa gtacgtgcca aacgtaatga tataggtttg
     1861 accgaaggct ttgatattga aaatgcatat ccttgcttcg gtaatgatga tgaccgcgta
     1921 gaccacttag gggtagattt ggtttatttc ttcagtgaag aattaaagaa acatccggta
     1981 tacaaaaatg cccgccctac cctgtcactg ctgactatca cctccaatgt aatgtacggc
     2041 aagaaaacag gtgccactcc tgatggtcgt gccaaaggag tggctttcgc tccgggtgcc
     2101 aacccgatgc acggacgtga caagaacggt gccatcgcct cattgagttc tgtagccaaa
     2161 ctacgctatc gtgatgccca ggacggcatc agtaacacgt tctctatcgt accaaaatca
     2221 ctaggggtag accaggaaac ccgtatagag aacctggtaa ccatgatgga cggttatttc
     2281 accaaaggag cacatcatct gaatgtaaat gtgctgaacc gtgaaatgtt ggaagatgca
     2341 atggaacatc ctgaaaaata cccgcaacta accatccgcg tatcaggtta tgccgtaaac
     2401 ttcatcaaat tgagccgtga acatcagctg gaagtaatca gccgttcttt ccacgaaaga
     2461 atgtaacatc acatatacat tcatcgtagg ggcgggtttt aaacctgccc ttaccagttt
     2521 aaagacaaac cattatgatc agagtccatt cttacgagtc aatggggacg tttgacggtc
     2581 ccggtttacg cttagtcgta tttttacaag gatgcaattt ccgatgcctc tattgtgcca
     2641 accccgacac aattgacacc aaaggtgaaa gtacggaaac aacaatagac gaaattgtgc
     2701 gtatggccgt cagccaaaaa gcttttttcg gtaaaaaagg aggtgtgact ttcagcggcg
     2761 gcgagcctac attgcaagca aaagcactga ttccgttatt ccagcgatta aaagaacaaa
     2821 gcatccacat ctgtatagat accaacggca gtatatggaa tgaagaagta aaggaactgc
     2881 tacaatggac agacctcgta ttattagaca tcaaggaatt taataatgtg cgccatcggc
     2941 aactgacaga acgcagcaac gaacaaacca tacgtaccgc cggatggctt gaaaaaaacg
     3001 gaaagccttt ttggttgcgc tatgtattag ttccaggcta tagttccttt gaggaagata
     3061 tacgggcatt aggagagcac ttcaagaatt atcaaatgat tcaaagagta gaaatactac
     3121 cttatcatac attaggcgta cacaaatatg aagccatggg aaaagaatac aaactaaacg
     3181 gaataaaaga aaatactcta aaacaattgg aaacagccaa aacgttattt ggtgaatatt
     3241 tcaataccgt atatttaaat tagaaatata ctgaagacaa gatgttgcta taaatcaatg
     3301 gaaatatttc atcatttcaa taaaattatc taatttcgca tctaaaacat aaattattat
     3361 aaatatgaaa agactactat tactctgcct aacttacatt atgtgtgtag gcaaccatca
     3421 gatggctgct caagactctt ctgaaagtcc tggtattttt aattcattag ctgtaggcgt
     3481 cagtgcaagt accacaggta taggtattga tgtagccagt cctataggaa gttacttggc
     3541 tgtacgtgca ggagtttctt ttatgccgaa ctttacttat tctacagatg tggatgtaga
     3601 tattgaaggt tcttatgaag gaaatatacc taccagcata gatgtagaag gaggaatggg
     3661 acgtactgcc ggtgaaatat tgctgaatgt atatcctttc aaatcatcta gtttttttgt
     3721 atgcggtggt gcttatttcg gaggaggaaa actcatcaag attaaagggc acagcgatga
     3781 gttggcagaa ctgattaaag aaggacaaga agtaggtatt gaaatcggaa actatcgtat
     3841 acctgtagat cagaacggaa atatatctgg tggtttgaag gtttcttcat ttcgtcctta
     3901 tcttggacta ggtttcggaa gagcgattcc taagaaacga attggcttca tgttcgaaat
     3961 gggtgtgcaa ttccataaca ctcccaaagt atataccaat aacggagatt tgtctgattt
     4021 aacagcagaa gctgataatg atttcactga tatcatagac aaacttactg tctatccggt
     4081 acttaagttc cgtttgtgtg gcaaaatatt ctaataatat tctataaaat aaaaggagat
     4141 gagccctata attttcgcct catctccttt ttatatataa gaaacaatac acttatatca
     4201 gcaccaccat cacatcacgt gccccatgag cacccattac caaagcctgc tcaatatcag
     4261 ccgtctttga cggaccggaa ataaaggttc cgaagccata ttcgcctgta tcaatcagtt
     4321 tatatgcttc atgcatattg ttcacaatct tattcttgtt tagaagaata accaattttt
     4381 cggcaataaa ataaatggca cgctgcttta catcctgttc tatccatacc gcaccattct
     4441 ccgcaacccc aatctttcca tctataacgg ccaaatccgt cccattcagt tctgcagggt
     4501 cttccacatc atcgggatta aatgtcgcac atgtaatagt cttcagatta gaagcgatgc
     4561 gtttggctcc aggataagcc tttttaatga aatcattcac gtcctcctct ttctccaaaa
     4621 gaatggcttc ccctcctacc tgcttcatga tgagataaaa ctgttccacc ttactgggat
     4681 aagttaatgc catatcttcc aatggcttta aatccggttt ctcatatttt acatgtgtag
     4741 cacgccggat gctctgcaat atatcttctc tgctgctcat aattacttca ctttaccttt
     4801 cttccacatt tcattgaaac tctccttagc aaatttcggc atctcacgcc cttttcccca
     4861 gtcattcaat ccgttataga taagaaaacg tggcattccg tttaccaatg gcgcccattt
     4921 taatgccata ttgaatatca aagggcgatt catcatgaat tccatgccac cggacattac
     4981 ccttttcatc cggtctgcct tacccaattt atccaaatcc tgacgccaca gatagatctg
     5041 ctccgccaaa tccaccttag ccggacacac aaacgaacag gaataacaca aagaacaagc
     5101 cgacacattg tcataataat gcaacggcgc cttcaacatt cccaggttaa tcccgatagg
     5161 gccgggaata aaataagtat aagagtaccc cccactacgt ctatatacag gacatgtatt
     5221 catacaggct ccacaacgga tacaattcaa agttttgata tgcttttggt cggccaatat
     5281 cttactacgt ccgttatcta caataataat gtgaagctcc ccaccctctc taggctttcg
     5341 gtaatgagaa gtatatgtag taatgggttg tcctgtagcc gaacgggcca acaagcgggt
     5401 gaacactcct aaagattcac ggtccggcac aattttttcc ataccaaaag ctgcaatctg
     5461 taatttaggc tgggaagtac ccatatccgc atttccttca ttggtacata ccacaatctc
     5521 accagtagaa gctacagcaa agttagcacc tgtcatagcc gcctcagcat gaataaattt
     5581 ctgacggaga ttcttccggg ctgcatgtgt aaggtaagta gggtcaaaat ttcctttctc
     5641 cgtacccatc tctcgctcga aaagctcacc tacctgttca cgtttgatat gaatagcggg
     5701 taatacaata tggcttggct tcagatgcat caattgcaag atacgctctc ccaagtcact
     5761 ttctaccact tcaattcctt tgttttccaa aaaagggttg agttcgcatt cctcggccaa
     5821 catggatttg ctttttataa aatgcttcac tccgtgctgg ttcagaatat tgtagacaat
     5881 attacaatac tcatccgcgt cctttgccca atagacatgg gctccattag cacgcgcatt
     5941 gttttcaaac tccaccaata attcatccaa atggctgtta ctatatagtt tagtagcaca
     6001 tgccatatta cgcaattcct cccattcggg aacttcttta ctcagtctgt cacgctttgc
     6061 cctaaccagc caaagtgttt cgtcatgcca tgcggcctgt ttcttgtttt tcagaaactc
     6121 cgcagccgct tttgaatgtt tcgtactcat aatcctgccg ctaagatttc aacaatgtgc
     6181 aatgtcttga taggaagttt gtcacgggca atgatgccat tctgatgcat caggcacgaa
     6241 ctgtcggccc ccacaatgta ttcagcaccg gtagcgatat gacgcatcac cttgtcatgc
     6301 cccatctgca cggaaaccgc atcttcctct acggcaaaca taccgccgaa accgcaacat
     6361 tcgtctttac gttccggttc cacaacttcc actccttcta ccaaagagag caaatcacgc
     6421 agtttgctga aataaggaat attaagttcg ctggcagaag aaagtttcat caatctcact
     6481 ccatgacaac tgttttgcag actcacttta tgtggaaact tggctttcaa cgaagaaggt
     6541 ctgacgatat catgaataaa ctcacagata tcatatatct taccttcgct ggcgcaccga
     6601 tgtttgtctt ctttcaacaa acgactgtag ttatcacgca caaaaacgac gcaactggcc
     6661 gaaggaccta ctatataatc gtaattctta aactgttcgt caaaatggcg ggctaaatct
     6721 accgctttat tctcgaaacc tgcattcgcc atcggttgac cgcaacaagt ctggttgaga
     6781 ggataatcca cgtccactcc cagacttttc agcagcttgt acgaggctat ccccacctgc
     6841 ggatataccg cattgatata acaaggaata aataaaccta ctttcattgt cattagttaa
     6901 tatgtgtcct atactgaata acaaagttag aggatatttg ttaagaaaac aacgagattt
     6961 ctaacgaata atgaagcgga ataggtaaaa aaacataata ttgtgggtaa aagccttact
     7021 aataaaaaag cactccccgc gaatcacttc gcagggagtg cttttaact
//
