LOCUS       gnlProkkaBNNNKGJG_251   6428 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BNNNKGJG_251.
ACCESSION   gnlProkkaBNNNKGJG_251
VERSION     gnlProkkaBNNNKGJG_251
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-12-01 06:50:14
            Original ID  :: gnl|Prokka|BNNNKGJG_251
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6428
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6428
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[756:6426]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      757..6426
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..6428
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..6428
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(138..554)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg251_1"
                     /sec_met_domain="Bac_transf (E-value: 2.4e-37, bitscore:
                     116.8, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVGADKKGLITVGGRDPRITRTGYLIRKYKLDELPQLFNVLKGD
                     MSLVGPRPEVRKYVDLYTDEQKKVLSVRPGITDYASIEYVDENMILGEASDPDRAYIEQ
                     IMPDKIRYNMKYICNRSVKEYFKIIFLTFWSIIR"
     CDS             complement(757..1980)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     267.1; E-value: 4.4e-81)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg251_2"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 8.4e-44,
                     bitscore: 138.9, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.4e-08, bitscore:
                     21.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIPFSPPYIDEAVINEVVDSLRSGWITSGPKVKALEEEIKSFSG
                     AKEVLCVNSWTSGAIMMLRWLGVKEGDEVIVPAYTYSATALAVLHAGAKPVMVDSGTDF
                     NILVEAVRKAITPKTKAIIPVDIAGFPCDYERIMALVQEPEMVKLFRSESPVQEKLGRI
                     LVMNDAAHSLGARYSSRQRTGCETDVAIFSLHAVKNVTTAEGGAICLNLPKPFDNTELY
                     KELRMTSLNCQTKDAFSKSKAGGWRYDIVGFGMKINMADVNAAIGLAQIREYPELLKER
                     KRVFNAYSDAFSACDWAIVPPSVDGEKESSYHIYALRIKDFTEEQRDRMIDEIAKSEVA
                     VNVHFIPMPMLSFFKSMGYDIKDYPQAYQNFKSEISLPIYPQLDSEKLNFIIETVKVAY
                     ATVIAENR"
     CDS             complement(2009..2821)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg251_3"
                     /sec_met_domain="Glycos_transf_2 (E-value: 5e-23, bitscore:
                     70.5, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLCVIMSIYKNDNLLAVTQSIESILRQAYEKIEFYIQLDGEIRS
                     DVLQYLRGLCIQDIRVKILNRTLNRGLAYSLNELLNIVLTKEYEYIARMDADDISMPER
                     FVKQIAFMNSHPDIDCLGTWAIEIDDDGKEYFRKKMPITHEECLELFKKRDCMIHPTVM
                     FRRSYFEKAGLYPEDTYFGEDTMMWAKGFKSGCKFANVPEYLFKFRLDSNFFERRRGWK
                     HAKSIYTLRHRVNRMLGFGWKEDCYALLYAMAKLMPKSILDIIYKTVR"
     CDS             complement(2823..3944)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Fe-ADH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg251_4"
                     /sec_met_domain="Fe-ADH (E-value: 6.9e-76, bitscore: 244.0,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCLNNDPFEYLQSNLSRYSSRKLFLVRGKKSYELCGAKRVVEDVI
                     SNLKCGFVEYFDFSENPKYEDLERGLKLLELSGADLIVAIGGGSVLDMAKLIRFFHSYS
                     GDILNEKFVKNRDLLPLFAIPTTAGTGSEVTHFAVLYKDNLKYSVSHKDMLPDRAIICP
                     QFTYKNARYLTACTGFDALSQAIEAYWNVYSIEESDNYSIKAIKLIWDNLPLVIDSSTN
                     ELRDKIAEGAYWAGKAINITKTTAPHAFSYPFTINYGYPHGHAVALTFPFFMRYNYAEL
                     ENKLHPLLNSKSHCKKMEQLYNMLGSTFDMIYDDMYNYLERIGLAINIRGRNFDKNLIL
                     HGINASRLSNNPCVLSQADVDFIVDEFVENGKL"
     CDS             complement(3931..5094)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 73.2;
                     E-value: 3.2e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg251_5"
                     /sec_met_domain="TPP_enzyme_N (E-value: 9.8e-16, bitscore:
                     46.5, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.1e-22, bitscore:
                     68.3, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSPKFFVETLQTYSMDFFTGVPDSLLKNMCAYITDHIESQNNII
                     AVNEGTALGLAAGYYIATGCIPIVYMQNSGIGNTVNPLLSLTDKVVYNIPVLLLIGWRG
                     EPGIKDEPQHIKQGMITIPLLDTLGIKNQILNKDPNMAKSQINDAIEYMRMTKEAFAFV
                     IQKDTFEEYKLQNTEDSKFDLDREEAIKIVCNSLDKGSVIVSTTGMISRELFEYRESID
                     ANHETDFLTVGSMGHASQIALGIALRRKNKKVYCFDGDGAVLMHMGALTTIGTSRAVNY
                     IHIVFNNGAHDSVGGQPTVGLKVNLSKIASACGYNNVISVDSKATLKESLDRFKSINGP
                     VLLEVKVRKGARKDLGRPTLTPVKNKELLMNFLEEADESDKSDNVFK"
     CDS             complement(5104..6426)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 203.8;
                     E-value: 5.3e-62)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg251_6"
                     /sec_met_domain="CTP_transf_like (E-value: 1.2e-16,
                     bitscore: 50.0, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2e-119, bitscore:
                     386.5, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 8.9e-66, bitscore:
                     210.4, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEMDIEKKKTVYVGMSADIIHPGHLNIISEAQRLGKVTVGVLTD
                     AAIASYKRLPYLNYEQRSLIVKNIKGVDEVIPQTTLDYVPNLKLLKPDFVVHGDDWKEG
                     IQKETRQKVIDTIAKWGGKVIDIPYTKGISSTQLNEKLKEIGTTPEVRLKRLRRLIEAK
                     SVVRICESHSGLTGLIIENTVVEVNGVKREFDGMWSSSLTDSTSKGKPDIEAVDLTTRL
                     HDLNDALECTTKPIIFDGDTGGKIEHFVFTVRTLERLGVSAVIIEDKVGLKKNSLFGTD
                     AVQLQDTIEGFSEKIKAGKLAQITTDFMIIARIESFIAGKGLEDAIERALAYVEAGADG
                     IMIHSKDKSGEDIKKFCYAFRQLNKSIPIVVVPTTYNHITETELASWGVNVIIYANHLL
                     RSAYPAMLNTAKIILANERSFEANDLCMSVREILELIPGTK"
ORIGIN
        1 ggatcggcaa ttgccgatcc tttctttttt attgaattaa cttctcttcg gaacttccgt
       61 ctactcctta ttctttctca cataataggg tacgaaaaca aaaagaagaa cttcccccag
      121 tttccttaaa aacataccta acgaatgatg ctccaaaatg tcagaaatat aattttaaaa
      181 tattccttca ccgaacgatt acagatatat ttcatattat accgtatctt atcgggcata
      241 atctgttcta tatatgctct gtcaggatcc gaagcctctc caagaatcat attttcatct
      301 acatactcaa tagaagcata atccgtaatc cccggacgta cactcaatac tttcttttgt
      361 tcatcggtat ataaatccac atacttacga acctcaggac ggggccccac cagactcata
      421 tctcctttca gtacgttaaa caactgaggc aattcatcca atttatactt ccggatcagg
      481 tatccggtac gagttattct cggatctctc cctcctaccg ttatcaaccc ctttttatca
      541 gcccctacac gcattgaacg aaatttataa acataaaaat cccctccgta ccttcccaca
      601 cggagctgtt tatagaaacc acctccttta ctttcgagac aaatggctat ataaagaaga
      661 agaaaaacgg gagaaagcag aagaattccc aaaagggaaa aaacgatatc aaaaaaacgg
      721 atcaagaaac ttatctattt agtgtttgta aatatactat cggttttctg caattacggt
      781 cgcataggcc acctttacag tttcaataat gaaattcaat ttttcactat ccaattgggg
      841 atatatcggc aatgatatct cgctcttaaa gttctgataa gcttgtggat aatcctttat
      901 atcatatccc atcgatttga agaacgacaa catcggcatc ggtataaagt gcacattgac
      961 agccacttca cttttagcga tttcatcaat catccggtct cgttgttctt cggtaaagtc
     1021 cttaatacgc aaagcataaa tgtgataaga gctttccttt tccccatcca cagaaggcgg
     1081 aacgatggcc cagtcacaag ccgaaaaagc atcgctataa gcattaaaca cccgctttcg
     1141 ttctttcagc aattccggat attctcgtat ctgtgccagt cctatggcgg catttacatc
     1201 ggccatattg attttcatcc cgaaaccgac aatatcataa cgccatcctc cggctttaga
     1261 tttcgaaaaa gcatccttgg tctggcagtt caagcttgtc atccgtagct ctttatacaa
     1321 ctccgtatta tcgaacggtt taggcagatt caggcaaatg gccccacctt cggcagtagt
     1381 cacatttttc acggcatgaa gtgaaaagat agccacatca gtttcacaac cggtacgctg
     1441 acggctactg taacgtgctc ccaaagagtg ggcagcatca ttcatcacca agatgcgtcc
     1501 caatttttct tgcacaggag attccgaacg gaacagtttt accatttctg gttcctgcac
     1561 cagtgccatg attctttcat aatcgcaagg aaaaccagca atatctacgg gtataatcgc
     1621 ctttgtttta ggagtaatag ctttacgaac agcctccacc aaaatattaa aatcagttcc
     1681 ggaatccacc attaccggtt tggctccggc atggagcaca gccaatgccg ttgcactgta
     1741 agtataggca ggaacaatca cttcatcgcc ctccttcact cccagccaac gcaacatcat
     1801 aatagctcct gatgtccagg aattgacaca aagcacttct tttgctcctg agaaggattt
     1861 aatttcttcc tccaaagcct tcactttcgg accggatgtg atccaaccgg aacgtaacga
     1921 atcaacgact tcgttgatga ccgcctcgtc aatataaggt ggtgaaaaag gtattttcat
     1981 gagtttatgt tttataagaa aagtaaaatc aacgtactgt tttatagatt atgtcaagaa
     2041 tagatttagg cattaatttg gccatagcat atagcaatgc ataacaatct tccttccatc
     2101 cgaaccctaa cattctatta acacgatgac gtaaagtata aatagatttt gcatgtttcc
     2161 acccccgcct tctttcaaag aagtttgaat ccaatctaaa cttaaacaga tattcgggta
     2221 cattggcaaa tttacatcca cttttaaaac ctttagccca catcatagta tcttctccaa
     2281 agtaggtgtc ttcgggatat aaaccagctt tctcaaaata tgaacgtcga aacatcacag
     2341 taggatgaat catacaatcc cgctttttaa acagttccaa acattcttca tgagttatcg
     2401 gcattttttt tcgaaaatat tcttttccgt catcatcaat ttctattgcc caagtaccaa
     2461 ggcaatcaat atccggatgt gaattcataa atgcgatctg cttcacaaaa cgttccggca
     2521 tggaaatatc atcagcatcc atacgagcaa tatattcgta ttcttttgta agaacgatat
     2581 tcaataattc atttagtgaa taagctaatc ccctattcaa tgtccgattt aatattttta
     2641 cccgtatatc ttgaatacac agtccccgta aatattgaag aacatccgaa cgtatttccc
     2701 catctaattg tatgtaaaat tctatttttt cataagcctg ccttaaaatg ctttctatag
     2761 actgagttac agctagcaga ttgtcatttt tataaataga cataatcaca cagagcttca
     2821 tattacaact ttccattttc aacaaattca tccactataa aatctacatc tgcttgagat
     2881 aatacacaag gattattaga taaacgagaa gcatttatac catgtaaaat aagattttta
     2941 tcaaaattcc ttcctctaat attaatagct aagcctattc tttctaaata attatacata
     3001 tcatcataaa tcatatcaaa agttgatccc agcatgttat ataattgctc cattttttta
     3061 caatgacttt tactattcaa taaaggatgc aacttatttt ctaattctgc ataattatat
     3121 ctcataaaaa aaggaaatgt taatgctact gcatgtccat gagggtagcc atagtttatc
     3181 gtaaaaggat atgagaaagc atgaggcgct gtcgtttttg ttatattaat agccttacca
     3241 gcccaataag caccttctgc tatcttatct cttaattcat ttgttgagga atcaatcact
     3301 aaaggaaggt tatcccatat taactttata gctttaatag agtaattatc agattcttca
     3361 attgagtaaa cattccaata agcttcaata gcttgtgata aagcatcaaa gccggtacac
     3421 gctgtcaaat atcttgcatt tttatacgta aactgaggac aaattatagc cctatcaggc
     3481 aacatatcct tatgagaaac cgaatatttt aaattatcct tatataaaac agcaaaatga
     3541 gtcacttcac taccagttcc tgctgtagtt ggaattgcga ataaaggtaa taaatctcta
     3601 tttttaacaa atttctcatt taatatatct cctgaataag aatgaaaaaa gcggattaat
     3661 ttagccatat ctaaaacact tccaccacct atggctacta ttaaatccgc cccacttaat
     3721 tccagcaatt tcagccctct ctccaaatcc tcatacttag gattttctga aaaatcaaaa
     3781 tattcaacaa acccacactt taaattagaa ataacatctt caacaactct ttttgctcca
     3841 cataattcat aagacttttt gcccctaacg aggaataatt tccgagagct atatctagat
     3901 aaattactct gtaaatattc aaatggatca ttatttaaac acattatctg atttatctga
     3961 ttcatctgct tcttctaaaa agttcattaa taattcctta tttttaacag gagtcaaagt
     4021 aggacgtccc aaatcttttc ttgccccttt tctcactttt acctccaata atacaggtcc
     4081 atttatactt ttaaacctat ccaaactttc ttttaaagta gctttactat ctacagatat
     4141 aacattatta taaccacaag cagaagcaat tttagagaga ttgaccttta atcccactgt
     4201 tggttgccca ccaacagagt catgcgcacc attattaaac acgatatgaa tataattcac
     4261 agctctacta gttcctatgg tagttaatgc tcccatatgc ataagtacag caccatcacc
     4321 atcgaaacaa tacacttttt tgttctttct tcggagagct atacctaaag ctatctgtga
     4381 tgcatgcccc attgacccta ctgttaaaaa atccgtttca tgatttgcat caatagattc
     4441 ccggtattca aataattccc tagaaatcat cccagtagtt gaaactataa cactaccttt
     4501 atctaaagaa ttacaaacta ttttaattgc ttcttctcga tccaaatcaa atttactatc
     4561 ctctgtattt tgaagcttat attcttcaaa agtatctttt tgaatgacaa aagcgaaagc
     4621 ctcttttgtc attctcatat attctattgc atcatttatt tgactttttg ccatattagg
     4681 atctttatta agaatttgat tctttatacc aagagtatcc aaaagaggaa tagtaatcat
     4741 gccttgttta atgtgttgtg gttcatcttt tataccaggc tcaccacgcc accctatcaa
     4801 caacaataca ggaatgttat aaacgacttt atccgtcaaa gacaacaaag ggtttacggt
     4861 attaccaata cctgaatttt gcatatacac aataggaata caccctgtag caatataata
     4921 tccggcagct agtccaagcg cagttccttc gttcactgca ataatattat tttgagattc
     4981 tatatgatct gtaatataag cacacatatt cttcaataaa gagtcaggaa ctccagtaaa
     5041 aaaatccata gaatatgttt gcaacgtttc tacgaaaaac ttaggtgata acataatttc
     5101 caactattta gtaccaggta ttaattctaa tatttccctt acagacatac ataaatcatt
     5161 tgcttcaaac gaacgttcat tagccaaaat aattttagca gtattaagca ttgcaggata
     5221 agcactccga agcagatgat tagcataaat aataacattt actccccaag aagccaattc
     5281 tgtttccgta atatgattat aagttgtagg cacgactaca attggtatag atttattcag
     5341 ttgcctaaat gcataacaaa attttttaat atcttcccct gacttatctt tactatgaat
     5401 cattatacca tctgctcctg cttctacata cgccaaagct ctctctatag catcctcaag
     5461 ccctttaccc gcaataaaac tttcaatgcg tgcaatgatc ataaaatcag tagttatttg
     5521 agccagcttt ccagctttta tcttctcaga aaaaccttct attgtatctt gaagttgaac
     5581 agcatcagta ccaaataacg aatttttctt caacccaact ttatcttcta ttataacagc
     5641 tgaaacacct aaacgctcta atgtacgcac agtgaataca aaatgctcta ttttcccccc
     5701 cgtatcgcca tcaaatataa taggcttagt tgtacactct aaagcatcat ttagatcatg
     5761 aagacgagta gttaaatcta cagcctctat atcaggctta cccttagatg tagaatcagt
     5821 cagactactt gaccacatac catcgaactc gcgttttact ccatttacct caacgactgt
     5881 gttctcaatt atcagccctg tcagcccact atgagattca caaatacgta caacagactt
     5941 agcttcaata agacgtctca acctctttaa acgaacttca ggagttgtac caatttcctt
     6001 tagcttttca ttaagttgag tagaagatat tcctttagta taaggaatat caatgacctt
     6061 tcctccccat tttgcaattg tatcaataac tttttgccta gtttcctttt gtataccttc
     6121 tttccaatca tcgccatgaa caacaaagtc aggctttaac aatttcaagt taggtacgta
     6181 atcaagcgtt gtctgtggta taacctcatc cactccctta atatttttaa caattaaaga
     6241 gcgctgctca taattcaaat aaggtaatcg tttgtaacta gctatcgcag catctgttaa
     6301 aactccgacc gttaccttac ctaatctctg ggcttctgaa ataatattta agtgacctgg
     6361 atgaataatg tcagcactca tcccaacata cacagttttc ttcttttcta tatccatttc
     6421 tttacttg
//
