LOCUS       c00223_gnlPro..         6038 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HMDHHGGE_223.
ACCESSION   c00223_gnlPro..
VERSION     c00223_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:04:06
            Original ID  :: gnl|Prokka|HMDHHGGE_223
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6038
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6038
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1651:6037]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1652..6037
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6038
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6038
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(17..1357)
                     /locus_tag="ctg223_1"
                     /transl_table=11
                     /translation="MKRIIILFFLCCTIPLIAQHTDPIQEAMANYDYETALSLIAQKKT
                     TPPLLLQKGKALRGLGLTTEALATYQEIICNDTTNTRAFIEAAECCRALAKYNQALKYY
                     EHALDLNPENKYVRIQYISLLLSQQKFSEALGESSLMTEKDSSAIVLHLQAQSFEGMGE
                     LLPAAGCYYNIQAKYPDDYLAASKLGALNISGSYFDEAIEATEKYRQIDSTNISVNRQN
                     ALAYCLKQDYPTAIQRYEYLVSQGDSSFHTCYYLGISYYAAEKYYEAHDFLEVARKYDP
                     NNINLLYYLGRACSKTSWKKEGVDYLEKAIDLSIPKDSSMTRLYIGMTDCYKMAQMYKE
                     QIASIKERYQRYDKQNHKLLYDMAYIYFYDLKDKKNAERYLEAFLKTRPKDTKEEAEMN
                     ERGELVLGKSNYYNAAANWLKDIQSKQKIEDFFQNSSPQLPLPKKDK"
     CDS             complement(1652..2812)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_2"
                     /sec_met_domain="OAD_beta (E-value: 3.5e-154, bitscore:
                     501.6, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFINFLGNNLADFWTYTGFANATVGHVVMILVGLVFIYLAIAK
                     EFEPMLLIPIGFGILIGNIPFNMDAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYMIALEMGFDPMQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPVIQPPIMRLLTTKHERVIRMKPPRAVSHTEKVIFP
                     IIGLLLTCFLVPSGLPLLGMLFFGNLLKESGVTRRLANTASGPLIDTITILLGLTVGAS
                     TQASEFLTLDSIKIFALGALSFIIATASGVIFVKIFNIFLKKGNKINPLIGNAGVSAVP
                     DSARISQVIGLEYDSTNYLLMHAMGPNVAGVIGSAVAAGILLGFLM"
     CDS             complement(2814..3245)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 67.7; E-value:
                     2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 2e-22, bitscore:
                     67.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEYKYKINGNSYKVTIGDIEDNIAHVEVNGTHYKVEMEKQPKPV
                     AKPVTVRPMPNAPAAPTQVVKPTAPSTGKSGVKSPLPGVILDIKVNVGDTVKKGQTIII
                     LEAMKMENNINADKDGKITAINVNKGDSVLEGNDLVIIE"
     CDS             complement(3270..4190)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_4"
                     /sec_met_domain="OAD_gamma (E-value: 3.3e-16, bitscore:
                     48.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKTKIGIFLSLLLLIGLTSCGEQKSNNKLVLNEILIDNQSNFQD
                     DYGLHSAWIEIFNKSFGSADLAACLLKVSSQPGDTVTYFIPKGDILTLVKPRQHALFWA
                     DGEPNRGTFHTSFKLNPETANWVGLFDSGKKLLDQIVVPAGALGPNQSYARVSDGAAEW
                     EVKSGSGDKYVTPSTNNKTLDSNSKMEKFEEHDADGVGMSISAMSVVFCGLILLFIAFK
                     IVGKVAVNLSKRNAMKSKGIDKHEAKELSQAPGEVYAAISMALHEMQDEVHDVEETVLT
                     ITRVKRSYSPWSSKIYTLRETPPRK"
     CDS             complement(4222..5775)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_5"
                     /sec_met_domain="CT_Btheta (E-value: 9.5e-291, bitscore:
                     953.0, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKIKELIERRAVARIGGGEKAIAKQHEKGKYTARERLAML
                     LDEGSFEEMDMFVEHRCTNFGMEKKHYPGDGVVTGCGTIEGRLVYVFAQDFTVSAGSLS
                     ETMSLKICKIMDQAMKMGAPCIGINDSGGARIQEGINALAGYAEIFQRNILASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMMEGTSYMFLTGPKVVKTVTGEDVSQENLGGASVHST
                     KSGVTHFTAQTEEEGFELIRKLLSYIPQNNLEEAPYVDCTDPIDRLEDSLNDIIPDSPT
                     KPYDMYEVIGAIVDNGEFLEIQKDYAKNIIIGFARFNGQSVGIVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPIVSLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTI
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVEVLYAREAKDQENPAQFLA
                     EKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLSNPAKKHGNIPL
                     "
     CDS             complement(5804..6037)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg223_6"
                     /sec_met_domain="Glyoxalase_4 (E-value: 8.4e-12, bitscore:
                     34.2, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EPTCPESTIAKFIENKGAGVHHVAFAVEDGVANALAEAESKEIRL
                     IDKAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
ORIGIN
        1 gctttatagt tcttatctat ttatctttct ttgggagagg aagttgtgga gaggaatttt
       61 gaaagaagtc ttctattttt tgtttacttt gaatatcttt cagccagttg gcggcggcat
      121 tataataatt ggattttcct aaaacaagtt cgcctctctc gttcatttcc gcttcttctt
      181 tagtgtcttt aggacgtgtt tttaggaatg cttccaaata gcgttccgca tttttcttat
      241 ctttcaagtc atagaagtat atataggcca tgtcatacaa tagtttgtgg ttttgtttgt
      301 cataccgctg gtagcgttct ttgatggatg caatctgttc tttgtacatt tgtgccatct
      361 tgtaacagtc ggtcatcccg atgtaaagtc gagtcatact gctatctttg ggaatggata
      421 gatcaatggc tttttccaga tagtctacgc cctctttttt ccaggaagtt ttggaacagg
      481 cgcgaccgag gtaataaagg agattgatat tgttgggatc atatttacgg gcgacttcca
      541 aaaaatcatg agcttcgtag tacttctctg ctgcatagta actgatgcct aaataataac
      601 aagtatgaaa agaactgtct ccttggctga caagatattc ataacgttgg atggcagtgg
      661 ggtagtcttg tttcaagcag tatgctaaag cgttctgccg gttgacggaa atgttggtac
      721 tgtcaatttg ccggtatttt tctgtagctt cgatggcttc gtcgaaataa gaaccggata
      781 tatttagagc gcctagtttg ctggctgcca gataatcgtc gggatattta gcctggatat
      841 tgtaatagca acctgcagcg ggaagaagtt cgcccattcc ttcaaagctt tgagcttgta
      901 gatgcaggac aatggcagaa ctgtcttttt ctgtcatcag actactttca ccaagcgcct
      961 cgctaaactt ctgttgggag agaagcaaac tgatatattg aatacgtaca tatttgtttt
     1021 ccggattcag atcgagtgca tgttcataat attttagtgc ttgattgtat ttagctagtg
     1081 ctcggcaaca ttcggccgct tcaatgaaag cacgagtatt tgtagtatca ttacagataa
     1141 tttcttgata ggtagcaagt gcctctgtag tgagtcctag tccacgcaac gctttgcctt
     1201 tctgtaatag caggggaggg gtagtctttt tttgagctat aagagaaagg gccgtctcat
     1261 aatcgtagtt tgccatagct tcttgaatag ggtctgtgtg ttgcgctatt agaggaatgg
     1321 tacaacaaag aaagaacaga atgataatcc gtttcataat attttatttt agttgtttga
     1381 cttatctgct gcaagttact aaaaatatta tatttacgaa ttattcgtag attattttgg
     1441 atagattaac ggaatgataa gagaaggtaa cacaacagtg cgcaatacaa tacatggggc
     1501 atggtagcta cagtcttttt tacagcaagt ccttataatg aaaaatcacc tttactacta
     1561 taatgtagca aaggtgattt ttataaattc aaggctttta ggatagtctt attttttatt
     1621 ttaagaactg ttcaaacagc ctccttatat cttacatcaa gaatccgagc aggataccgg
     1681 cagcaacagc tgaaccgatc actccggcaa cgttcggacc catagcgtgc atcaacaaat
     1741 agttggtcga atcgtattcc aaaccaatca cttgtgagat acgtgcagag tcggggacag
     1801 cagaaacccc tgcattaccg atcaatggat tgatcttgtt gcccttcttc aagaagatat
     1861 tgaatatctt aacgaagatt acaccggatg cagttgcaat gatgaatgac aatgcaccga
     1921 gggcgaaaat cttgatagaa tcgagcgtca ggaattcgga agcttgcgta gaagcaccta
     1981 ctgtcagacc caacaggata gtgatagtat caatcaacgg accgctagct gtattggcaa
     2041 gacgacgggt tacaccactt tctttcaaca ggttaccgaa gaacagcata cccaacaaag
     2101 gtagaccgga aggaaccagg aagcaggtca gcaacaagcc gataatcggg aaaataacct
     2161 tctccgtgtg agaaacggca cgcggcggtt tcatgcggat gacacgttcg tgcttggtgg
     2221 tgagcagacg catgataggc ggctgtatca ccggaaccaa cgccatatag gagtaggcgg
     2281 acaccgcaat ggctcccatc aggttaggtg ctagcttgga ggaaaggaag atggccgtcg
     2341 ggccatctgc tccaccaatg ataccgatag cgccggcttg catcgggtcg aagcccattt
     2401 ctaacgcgat catataagca ccgaagatac cgaattgtgc agctgcaccg atcagcatca
     2461 attttggatt agagataagt gccgagaagt ccgtcatggc accgataccc aaaaagatga
     2521 gtggcggata ccagccggag gtcactccct gatacaatat attcaataca gaaccttctt
     2581 cataaatacc gactttcagc ccggcatcca tattaaaagg tatattaccg atcaatatac
     2641 cgaaaccgat aggaatcagc agcatcggtt cgaactcttt ggctattgcc aaatagatga
     2701 ataccaagcc aacgagaatc atgactacat gccctaccgt agcattggca aagcctgtgt
     2761 atgtccagaa gtcggccagg ttatttccta aaaagtttat aaagtctccc atattattca
     2821 ataattacga ggtcattacc ttcaagaaca gagtctccct tgttgacgtt gatggcagta
     2881 atcttaccat ccttatccgc attgatattg ttttccatct tcatggcttc caatatgata
     2941 atggtctgtc ctttctttac agtatcgcct acattcactt tgatgtcgag gatcacaccc
     3001 ggcagcggag attttactcc tgattttccg gtagacggag cggtcggttt cactacttga
     3061 gtaggagcag caggagcatt aggcatcggg cgtactgtca cgggtttcgc aacaggcttc
     3121 ggctgtttct ccatttctac tttatagtgt gtaccgttca cttctacatg agcgatgttg
     3181 tcttcaatat ctccgatggt tactttatat gagttaccgt tgattttata tttatattct
     3241 ttcatcgttt ttacttttta aaaggtgact tactttctgg gaggagtttc acgcaaagtg
     3301 taaatcttcg aactccatgg tgagtagctg cgttttacgc gggtgatggt cagcaccgtt
     3361 tcttctacgt cgtgcacctc atcctgcatt tcgtgtaatg ccatagaaat agcagcgtat
     3421 acttcgcccg gagcctgtga cagttcctta gcttcatgct tgtcaatgcc tttagatttc
     3481 atagcgttac gcttgctcaa attaacagct acttttccta caatcttaaa agcaataaag
     3541 agaagaatca atccgcagaa tactacgctc atggcagaaa tggacattcc aacgccatca
     3601 gcatcgtgtt cttcaaactt ttccatcttg gaattgctgt caagagtctt gttgttggtg
     3661 ctcggagtca cgtatttgtc accgcttcca ctttttactt cccattcagc tgccccgtcg
     3721 cttacacgag cgtatgattg gttgggaccg agcgcgcctg cgggcactac aatctggtcg
     3781 agcaactttt tgccggagtc gaacagacca acccagttag ctgtttccgg attcagcttg
     3841 aagctggtat ggaaagtacc acggttaggt tcgccatctg cccagaatag tgcatgttgg
     3901 cgtggtttca ctaaagtaag tatatcacct ttcgggataa aataagtaac tgtatcgccc
     3961 ggttggctac ttactttcag caagcaagct gccaggtcgg cgctaccgaa tgatttattg
     4021 aatatttcaa tccatgcgct gtgcaatccg taatcgtcct gaaaattact ctgattgtct
     4081 atcaggattt cgtttagcac cagcttattg ttcgactttt gctctccaca agaagtcaaa
     4141 ccaatcagca acagcaaaga aaggaatatt ccgattttgg ttttgttcat aatcgttctt
     4201 gttttaatgt gaaaagtagg attacaacgg aatattacca tgcttcttag ccggattact
     4261 caatttctta gtctgcaact gctgcaaagc acggataaca cggaaacgtg tgttgcgcgg
     4321 ttcaatcaca tcgtcgatgt agccatattt agctgcattg taaggattgg cgaacaattt
     4381 cgtgtattct gcttctttct ctgcaaggaa ttgagccggg ttttcctgat ctttggcttc
     4441 gcgtgcgtac aatacttcta ctgctcctgc accacccatt actgcgattt cggcagtagg
     4501 ccatgcgtag ttcatgtcac cgcgaagttg cttacagctc atcacgatgt gagaaccacc
     4561 gtaggattta cgcaatgtga tagtaacctt aggcacagta gcttcaccgt aagcatacaa
     4621 cagtttagca ccatgaagga taacgccatt gtattcctga cctgttcccg gaaggaatcc
     4681 cggtacgtct accaacgata cgataggaat attgaatgca tcgcagaaac gaacgaaacg
     4741 tgcaccctta cgagatgcgt tactatcgag cacaccagcc agatacttag gctgattagc
     4801 aacgatacct accgattgtc cgttgaagcg ggcgaaaccg atgatgatat tctttgcata
     4861 atctttctgg atttcaagga attcaccgtt gtccacaata gcgccgatca cttcgtacat
     4921 gtcatacggt ttagtagggc tgtcggggat aatatcgttc aaagaatctt ccagacggtc
     4981 gattgggtct gtgcaatcta cataaggagc ttcttcaagg ttgttttgag gaatatagct
     5041 caacagtttg cgaatcagtt cgaaaccttc ttcttctgtt tgggcggtga aatgagtcac
     5101 acccgattta gtggagtgaa cgcttgcgcc accgaggttt tcctggctaa cgtcttcacc
     5161 tgtaacggtc ttcaccactt tcggtccggt gaggaacata taagaagtgc cttccatcat
     5221 cagtgtgaag tcggtcagag ccggagaata aacagcacca ccggcacaag gaccgaagat
     5281 accggaaatc tgcggaataa cacctgaagc aaggatgttg cgttggaaga tttctgcata
     5341 acctgccaaa gcgttgatac cttcctggat acgtgcacca cccgagtcgt tgataccgat
     5401 acagggagcg cccatcttca tggcttgatc catgatttta caaattttca atgacatcgt
     5461 ttctgacagt gaaccggcag aaacagtgaa gtcctgtgcg aacacatata ccagacgtcc
     5521 ttcgatcgta ccgcagccgg ttactacacc gtctcccggg taatgttttt tctccatgcc
     5581 gaagttcgtg catctgtgct caacgaacat gtccatttct tcgaagctac cctcgtcaag
     5641 cagcatagcc agacgctcgc gtgctgtgta tttccctttt tcgtgttgct tcgcaattgc
     5701 tttttcaccg cctccgatac gtgctacggc acggcgttca ataagctctt taattttttc
     5761 aagttgatta ctcatgagtt cttgttagtt ttagagttta tgattaatgt tcgcagagtt
     5821 ctgtcaacac acccagagtt gattttgggt gaaggaaagc gatgttcaaa ccttcggcac
     5881 ctttgcgcgg agctttgtcg attaggcgga tttccttgct ttctgcttct gccaaggcat
     5941 tggctacacc atcttccacg gcaaatgcaa cgtgatgaac acctgcacct ttgttttcaa
     6001 tgaatttggc aatagtactc tccgggcaag taggctcc
//
