LOCUS gnlProkkaJDDFBJCG_409 15932 bp DNA linear UNK 01-JAN-1980 DEFINITION gnl|Prokka|JDDFBJCG_409. ACCESSION gnlProkkaJDDFBJCG_409 VERSION gnlProkkaJDDFBJCG_409 KEYWORDS . SOURCE ORGANISM . COMMENT ##antiSMASH-Data-START## Version :: 7.1.0 Run date :: 2024-11-17 11:04:16 Original ID :: gnl|Prokka|JDDFBJCG_409 NOTE: This is a single region extracted from a larger record! Orig. start :: 0 Orig. end :: 15932 ##antiSMASH-Data-END## FEATURES Location/Qualifiers protocluster 1..15932 /aStool="rule-based-clusters" /category="RiPP" /contig_edge="True" /core_location="[6334:6601]" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="1" /tool="antismash" proto_core 6335..6601 /aStool="rule-based-clusters" /tool="antismash" /cutoff="10000" /detection_rule="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /neighbourhood="10000" /product="RRE-containing" /protocluster_number="1" cand_cluster 1..15932 /candidate_cluster_number="1" /contig_edge="True" /detection_rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /kind="single" /product="RRE-containing" /protoclusters="1" /tool="antismash" region 1..15932 /candidate_cluster_numbers="1" /contig_edge="True" /product="RRE-containing" /region_number="1" /rules="minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])" /tool="antismash" CDS 34..2388 /locus_tag="ctg409_1" /transl_table=11 /translation="MKRLILYIAGLIFLAGCSTTKHLPEGEILYTGQKPMIVLNRSETS VGEIAMEEVEAALATAPNNSLLGSSTIRYPFPFGLWIYNGFQKYEKGFGKWIFNKFAAT PVLMSTVNPDIRQKAAVNLLRDYGYFNGSVSYKTFIDPKDSLKAKLQYTVNMRNPYFID TVYYRGFSERTTRIMELGRRRSLISSGEQFNVADLDGERTRISTLLRNVGCYYFRPDYL TYQADTMMVPNGHVQMRLIPVPGMPKVAEKQFRVGRKSVYLLGKQGQEPNDSMDYKGLT IHYYNKPPVRPNMLYRWLNYQGYRRKRQIQDSAGIARQRSMQSLYSLYRQTRIQERLAS VGIFRYAEMQYIPRDTAFVSDTLDVRVIAALDKPYDAELDFNVTMKSNNQTGPGAAFTV TKNNVFGGGESWNVKLNGSYEWQTGKASSSLMNSYELGISTSLIFPRVVFPRMGDREYD FPATTTFRLYADQMNRAKYYKLLAFGGNVTYDFQPKSTSRHSITPFRLTFNVLRNPTAA FDTLRAENPALYVSLRDQFIPAMEYTYTYDNASVRGKRNPIWWQTTVASAGNLTSAVYR IFGKPFSEEGKKLFGVPFAQFLKLNSEFRYHYRIDKNQMIASRIAGGVIWSYGNATTAP YTEQFYIGGANSVRAFSARSIGPGGYPPETDRKYTYINHVGDIRIEANIEYRFRMIADL HGAVFLDAGNVWLMRKDENRPDGEFTLKNFPKQIALGTGFGLRYDLDFLVFRLDLGIGL HDPYDTGKSGYYNIPKFKDSMALHFAIGYPF" CDS 2514..3425 /locus_tag="ctg409_2" /transl_table=11 /translation="MKPTLFLLAAGMGSRYGGLKQLDGLGPNGETIMDYSIYDAINAGF GKLVFVIRKDFEQDFRDKIISKYEGHIPCELVFQSIDDLPEGFTCPEGRTKPWGTNHAV MMGADVIKEPFAVINCDDFYGRDSFQVMGKFLAALPEGSKNVYSMVGFRIGNTLSESGT VSRGLCGTDANNLLTSVVERTKIQRMDGEVKYIDDNGEWTATPETTPVSMNFWGFTPDY FAYSAEFFKSFLSDPKNMENLKSEFFIPLMVDKLINNGTATCEVLDTTSKWFGVTYPED RQSVVDKIQALVDAGEYPAKLF" CDS complement(3470..5140) /gene_functions="transport (smcogs) SMCOG1288:ABC transporter related protein (Score: 278.7; E-value: 1.7e-84)" /gene_kind="transport" /locus_tag="ctg409_3" /transl_table=11 /translation="MKKQQKDKKVKKDRREKLRYIREVSAEQRGRILLSCLTGILGVAF ALAFIYATKRVIDTATGITDDNLTHIAVLTVVLLTAQLLCGAADTWISTRMQIETGNAL RHRLFSRLLQSRWNELERFHTGDVVNRVEQDTTAIVTLLTSSVPAFIVTSIQLLAAFLF FYFLDPSLPWLIVAILPVFLSGSRFYMRRMKRYTHDIRNSDGRIQSVIQESLQHRIVIK TLEQGERHLDKLDDLQDMLHSQVMGRTRFSLSAHMLVALAFSGGYLTAFLWGAVHLSAG CITFGTMTAFLQLVGKVQRPVFDLSRLIPSVVNALTAIDRLLELERLPAEADGDSIFLA PTPELELKDVTFRYTPDDRPVFSRFSCRFPAGSCIAIVGETGKGKTTLIRLLLALATPQ EGQILLIPAKHPLETSHTVSPQTRKNFVYVPQGNTLFSGTIRDNLLMGNPQASQEAIYQ ALQTATADFVFHLPDGVDSPLSERGGGLSEGQAQRIAIARALLRPGNILLLDEATSALD PDTERLLIKNLRRDCAGKTCIFVTHHPAVAEACESIVRL" CDS complement(5163..6326) /gene_functions="biosynthetic-additional (rule-based-clusters) NTP_transf_5" /gene_kind="biosynthetic-additional" /locus_tag="ctg409_4" /sec_met_domain="NTP_transf_5 (E-value: 6.2e-46, bitscore: 146.8, seeds: 77, tool: rule-based-clusters)" /transl_table=11 /translation="MQHAFFSLVRSGLWGTPADRQLFRSVSPDDWETVYRMANSQALLA LTFDGISSLPAELRPPRVLYLKWAARTAQIEQANKRLNQLLPELNTLYREAGLHPVLLK GQGIGTNYRTPLHRQCGDIDIYLGKQGQPIANRLLLQQGAIVEGEASDKHASYSLKGVH IENHRIILRLNSPLANRYFQQIIRKWYPQETDYALFSETGKEDSKAVSIAIPPASFNAL YIFLHAFVHFLNSGIGLRQLCDWTCLLANRHKEIDATTLLRQLQDLGLLHAAQAFGYIA VTRLGLPANRLPFPLEGTKQIGEQLLEDILSTGNFGQHDNRIKPRPKGYWAGKWYTFCR ATRRCNELRQFAPYEALWYPVTLIGGTIAIQINRSKGVKDKKARTKK" CDS complement(6335..6601) /gene_functions="biosynthetic (rule-based-clusters) RRE-containing: Stand_Alone_Lasso_RRE" /gene_functions="biosynthetic-additional (rule-based-clusters) PF05402" /gene_kind="biosynthetic" /locus_tag="ctg409_5" /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.1e-13, bitscore: 41.0, seeds: 25, tool: rule-based-clusters)" /sec_met_domain="PF05402 (E-value: 5.2e-16, bitscore: 48.7, seeds: 461, tool: rule-based-clusters)" /transl_table=11 /translation="MKIKQGFILHRIGDDYIIMQDGSSNVDFSKVISLNQTSAMLWQEI ENKEFDTDSVTEALTSRYDVDNESARRDAQKFIDSLDREGIIE" CDS complement(6614..7054) /locus_tag="ctg409_6" /transl_table=11 /translation="MKKSIPNEILLPEVARLIKEGHTVTITVRGNSMNPFLVDRRDRVT LGDFTTEQLQPGVAVLARDLTERIVFHRIIRRNGDILILQGDGNLAQTEETDIGRVMGV MTEAMRKDKRYTADGKVWKLYSYWWMKLNPARRWLLALFRRI" CDS complement(7081..8007) /locus_tag="ctg409_7" /transl_table=11 /translation="MRIQILDYQVGPHTFRMVKPSDFNIFKALPSLIPFITTIDTTQKV IFETEIDDDETATKRTIAKTPDDICFNWEDADCIIRPLPHSSHLVSITPRKSGKNYWME CNDNFRQCFIHLPACRTETPAPENETNFVLNNFLMMLYAFNAARHHTLLMHASVVATET GKGYLFLGKSGTGKSTHTGLWLQQFSDCHLLNDDNPIVHVDSLGKQATVFGSPWSGKTP CYRKESMTVGAFVRLEQAPQNEIEQERAAHAFATLLPSCSCLKQNKEIYNAIVTTVTEL ATLAPVYHLKCLPDREATELCRKTVEG" CDS complement(8254..11832) /locus_tag="ctg409_8" /transl_table=11 /translation="MMKLRNLMQVACMATAALTAFSCSQEEFENSGRKGNITVNATFEG AGTDTRTTVNDEYKILWQDTDALGLFCSNAESNYSNTKLEYASGAGQTSATFNGSKPSG ETAVFSIYPYQQNMSVSGNTLTMTLPATLTNYNGSSNGPMYAKVTNPDNLSALSFKHMA AMIKLTVNKIPAEATTFKIIASNNIAGTCTVDLTAADPILAVTSDESKEITASFTASAD IKSRNFYIPLPTGTYSSITAQLTNGSDKVYFTKTLNDKILGRRDILVVPPLDCVVVEAT TPSALSTALADSKNLPQEAPTAATVTDIAVSGSFNTTSGSNDGIAIPVLQNSDINLAFN TAPTTSTAAPLTLTDKTNTSIGAPAATATNSVSLAVPETNAEQEAPSVAITMPSTTVTL AAVGNKATYNEVTATTAQQTLIINAGVTVKKLTVKGGNLKIYGKVEQLVHDAGDTTIYI IKGTEASLPATIDSKFVVQSDVAVLKAAFANGEDFKLSADADITGQSVSVPAGKSVVLD LNGYTLTADNSATGKIIVLGKMTLKDSSTEKKGKIVASQDYTAASYNGSLIEIAGEDAS MTMESGNISAVRKTPNSNGQYGVGVTDGGDFTMTGGKIEAGWFAVAGNGNYKTQNSIIN ITDGELISTADYAVYLPQSGTTTISGGKVYGAAGGVCIQRGTLNVEGTALITSKGTGST GNWGDGTGGLDCAAINVSGAYGIATVNIKGGTLIAEAKSLITEGTTYTPVINVTGGTFS DPSALKYMKANANVNIKLTADKTCPGFKTTSGQTLTMDLGGKILTLADPTVGSTGTETN SCQLLEGSNVTFKNGTLKSDNNKIMIQNYCNLTLDNMTVEDTNAQYVVSNNCGNISINN TTINAGSNANQFAFDVCGYAKYTAGVTVTVSGTSVINGKVEISKSAGNTEPMKLNITGG TFNGDLKVDASVGTENAKSIISVSGGTFSDPSVLKYMATNATVDIKLLSNINIAKTELA TGYILNAANATANLNLNGHDIINSSETADATPFTQIFTVQNGTLNISGNGNVKCDASAT AKDDGYRMVIEARGHGTVNIHGGSYYNTQKLNTQIDLIYARENGKINIYGGTFESGKYG TPNNDTDGRYWVLNLKNTDKNTASIQVSGGTFINFNPANPNMDDNESYLVTGYEVTRDG SVYTAAHKVNDGRKEYIVGPTSQENR" CDS complement(11846..12061) /locus_tag="ctg409_9" /transl_table=11 /translation="MKQNKPDGREAYITPSIEVIKMENEGVIATSGEPYHPITRSYSGS TSTHCYNSASSSDLEDLINDILTVEN" CDS 12111..12287 /locus_tag="ctg409_10" /transl_table=11 /translation="MFVYPLFRPSHCPFPGYTTVTHVVCVYTIPLMGENHINIDVSILV VIFREIFKVAKFE" CDS complement(12427..12786) /locus_tag="ctg409_11" /transl_table=11 /translation="MDICKNMESLIMKNCYGIDFRSWRKELRTREKTKQTPCKLPDRVS RSLQGVLFKVYQISVLILLKKILFSFFSVCLPLFIRHIAAMLLRKTSSKTMQTVTKYLK ELSGVRSDNSPNSYK" CDS 13619..13951 /locus_tag="ctg409_12" /transl_table=11 /translation="MKQLIACCGLDCENCAARIATVNNDDELREKTAKEWSVLNNTPEI TAETIHCMGCRADGVKFAYCSDYCAIRKCVYEKGFNTCGDCKELETCQVVGAVLQHAPG AKENLY" CDS 14185..14961 /NRPS_PKS="Domain: oMT (51-145). E-value: 6.2e-11. Score: 32.8. Matches aSDomain: nrpspksdomains_ctg409_13_oMT.1" /gene_functions="biosynthetic-additional (smcogs) SMCOG1089:methyltransferase (Score: 78.7; E-value: 7.7e-24)" /gene_kind="biosynthetic-additional" /locus_tag="ctg409_13" /transl_table=11 /translation="MSNENKTIHDFELSLICDFFSNMERQGPGSPEATLKALSFVDGLT DKSLIADIGCGTGGQTIVVAGHVPGRITGIDLFPGFIDIFNRNARQLGLQDRVKGIVGS MDALPFGEEELDMIWCEGAIYNIGFERGLKEWRKYLKPGGYIAVSESSWFTDERPAEIN DFWMDAYSEMDTLPNQVAKLYKAGYLPVATFILPENCWTEHYFASKVAAQEIFRKKYAG NKIAEEFSSLQMIEDELYGKYKEFYGYVFFIAKKVD" aSDomain 14338..14619 /aSDomain="MT" /aSTool="nrps_pks_domains" /database="nrpspksdomains.hmm" /detection="hmmscan" /domain_id="nrpspksdomains_ctg409_13_oMT.1" /domain_subtypes="oMT" /evalue="6.20E-11" /label="ctg409_13_oMT.1" /locus_tag="ctg409_13" /protein_end="145" /protein_start="51" /score="32.8" /tool="antismash" /translation="DIGCGTGGQTIVVAGHVPGRITGIDLFPGFIDIFNRNARQLGLQD RVKGIVGSMDALPFGEEELDMIWCEGAIYNIGFERGLKEWRKYLKPGGY" CDS complement(15036..15932) /locus_tag="ctg409_14" /transl_table=11 /translation="MFIHYYPPFLCLKISNMNISEFNINAEFIACNHITTNELNPLVEK PYHTKASIFALCTKGVLKTIINHTQFRVEANTLLVIPPETFVQLLHTSDDAEIYVVIFS QQLIQSSGAGKVMMDKFHIIGKHYIFPLSKTDFQLYAEFMTYLSHLYQRTESPSSLVSL QTLLTYLLQGISELCPERPRIKETPGSRHFNQYRIFIRLVHTDYVREHQVSYYALKMNM RPAALCRLVKKESGHTAMEIINNTIIMDAKAQLCTANTPIKDIAVSLGFNNAAFFNKFF KRHTGIPPQKFRTSSKQ" ORIGIN 1 gaaaatagcg atatgaaaaa gtaggaatgt gatatgaaac gactgatatt atatattgca 61 ggattgatat ttctggcagg ttgctccacc acgaagcact tgcccgaggg ggagatactc 121 tataccggcc aaaagccgat gattgtattg aaccgcagtg aaacgtctgt tggtgagatt 181 gctatggaag aggtggaagc cgcccttgct acggcaccga acaattcgtt gctgggaagc 241 tctactatcc gctatccctt tcctttcgga ctgtggattt acaatggttt ccagaagtat 301 gagaagggat tcggaaagtg gatattcaat aaatttgccg ccactccggt gcttatgtct 361 acggtgaatc ccgatatacg ccagaaggcg gcggttaact tgctgcgcga ttatggctat 421 ttcaacggaa gcgtcagcta caagacattt atcgacccga aggactcgct gaaggcaaag 481 ttgcaatata cggtgaacat gcgtaacccc tactttatag atacggtata ttatcgtggt 541 ttcagcgagc gtaccactcg cattatggag ctgggacgcc gacgttccct tatcagttcg 601 ggagaacaat tcaatgtggc tgatttggat ggggagcgta cccgtatcag taccctgctg 661 cgtaatgtgg gctgttatta tttccgtccc gactacctaa cctatcaggc cgataccatg 721 atggttccca atgggcatgt ccagatgcgc ttgattcccg ttcccggtat gccaaaggta 781 gccgagaagc agttccgtgt ggggcgtaaa tccgtctatc ttcttggaaa gcaggggcag 841 gaacccaatg acagcatgga ttacaaaggc ttgaccatac attactacaa caaacctccg 901 gtacgtccca acatgcttta ccgttggttg aactatcaag gttatcggcg gaaacgtcag 961 atacaggaca gcgcaggtat tgcccgccag cgttctatgc agagtttgta tagtttgtac 1021 cgtcagaccc gtattcagga gcgtctggca agcgtcggta ttttccgtta tgcggagatg 1081 cagtatatac cgcgtgatac ggcgtttgtg tctgatacat tggatgtgag ggtcattgcc 1141 gcgctcgata aaccctacga tgcggagttg gattttaatg tcaccatgaa gagcaataac 1201 cagacgggac cgggtgccgc ttttacggtg acgaagaaca atgtcttcgg tggcggtgaa 1261 agctggaatg tgaagttgaa cggttcgtac gaatggcaga cgggcaaggc aagttcttcc 1321 ttgatgaaca gttatgaact gggtatctcc acttcgctta tctttccgcg cgtggtgttt 1381 cctaggatgg gcgaccgtga gtatgacttc ccggctacaa cgactttccg tttatatgcc 1441 gaccagatga accgtgccaa gtattataag ctgttggctt ttggcggtaa tgtcacttat 1501 gacttccagc ccaaatcgac ttccaggcac agtataactc ctttccggtt gacattcaat 1561 gtactgcgca atcctactgc cgctttcgat acgttgcgtg ccgagaatcc ggcactctac 1621 gttagtttgc gcgaccagtt cattcctgcc atggagtaca cctatactta tgacaacgct 1681 tccgtgcgcg gcaagcgtaa ccccatctgg tggcagacca ccgttgcgtc ggccggtaac 1741 ctgacttctg ccgtctaccg tattttcggc aagccgttca gtgaggaagg aaagaagctt 1801 ttcggggtac cttttgcaca gttcttgaag ctgaactcgg aattcaggta ccattaccgg 1861 atagacaaga accagatgat agcctcgcgt attgccggag gggtgatatg gtcgtatggc 1921 aacgctacga ccgcacctta tacggaacag ttctatatcg gcggtgccaa cagtgtgcgc 1981 gccttttctg cacgcagtat aggtccgggc ggctatcctc ccgagacgga taggaaatat 2041 acctatatca accacgtggg tgatattcgc atagaagcca atatcgagta tcgcttccgt 2101 atgattgccg acttgcatgg agcggtattc ttggatgccg gcaatgtatg gctgatgcgt 2161 aaagatgaaa accgcccgga tggtgaattt acgttgaaga atttcccgaa acagattgct 2221 ttaggcacgg gatttggctt gcgttatgat ttggactttc ttgtttttcg tctggacttg 2281 ggtatcgggc tgcatgatcc gtatgatacc gggaagtcgg ggtattataa tattcccaag 2341 ttcaaggaca gcatggcgtt gcattttgcc atcggttatc cgttctaaat gtaccttttt 2401 gaacggatag tggtaaagaa tgaactaaaa ctcccttctg tcagccataa atttttgtac 2461 atttgcaacc gttatcaact gtgatacact aaccttttta aataataact aatatgaaac 2521 caactttatt cttattggca gccggtatgg gcagccgtta tggtggcttg aaacaattgg 2581 acggattggg accgaatggc gaaactatca tggattattc tatttacgat gccatcaatg 2641 ccggttttgg caagctcgtt ttcgtaatcc gtaaagattt tgaacaggac ttccgcgaca 2701 agattatttc caaatacgaa ggccatattc cctgcgaact ggtattccag tccatagatg 2761 accttcctga aggcttcacc tgccccgaag gccgtaccaa accttgggga accaaccatg 2821 ctgtaatgat gggtgccgat gtaataaagg aaccgtttgc tgttatcaat tgcgacgatt 2881 tctacggtcg tgactccttc caggtaatgg gcaaattcct tgcagccctt cccgaaggct 2941 ccaaaaacgt atattccatg gtaggcttcc gcatcggcaa tacgttgagc gagagcggta 3001 ccgtatcccg tggtttgtgc ggaacggatg caaacaactt gttgacttct gtagtagagc 3061 gtaccaagat tcagcgtatg gacggcgagg tgaagtacat cgatgataac ggtgaatgga 3121 ctgccactcc cgagacgact ccggtcagca tgaacttctg gggctttact cccgattact 3181 ttgcttacag tgcagagttt ttcaagagct tcctgagcga cccgaagaat atggagaatc 3241 tgaaaagcga attcttcatc ccgctgatgg tggataagct gattaacaat ggcactgcta 3301 cctgcgaagt gctcgataca accagcaagt ggttcggtgt gacttatccc gaagaccgtc 3361 agagtgtagt cgacaagatt caggctttgg tggatgcggg cgagtatcct gcgaagctgt 3421 tctgagtttg ttaaccaaag gcagataccc gttggggctt attctctgat tataatcgga 3481 caatagattc gcatgcctcc gctactgccg gatggtgggt gacgaagata catgttttgc 3541 ctgcgcagtc tctgcgcagg ttttttatta acagccgttc cgtgtcgggg tcgagggcag 3601 aagtggcttc atctaaaaga agaatgtttc cggggcgtag cagagcgcgg gcaatggcga 3661 tgcgttgggc ttgtccttca ctgagtccac cacctcgttc gctgagtggg gagtctacgc 3721 cgtcgggaag gtggaagacg aagtcggcgg tggcggtttg cagggcttga tatatggctt 3781 cttgagaagc ttgtgggttt cccatcagca ggttgtcacg gatagttccg ctgaaaaggg 3841 tattgccttg cgggacatat acgaagttct tgcgggtttg cggagagacg gtatgactgg 3901 tttcaagtgg atgtttggcg ggtatcaaga gtatctgtcc ctcttgtggc gttgccagtg 3961 ccaacaatag ccggatgagc gttgtctttc cttttcctgt ttctccgact atagctatgc 4021 agctaccggc ggggaaacgg caggagaagc ggctgaatac cggacggtca tcgggagtgt 4081 agcgaaaagt cacgtctttc agttccagtt ccggggtagg ggcgaggaat atgctgtcac 4141 catccgcttc tgccggcagt ctttccaatt ccaatagccg gtctatggcg gtaagggcat 4201 tgactacgga aggtatcagg cgtgagaggt cgaacacggg acgctggact tttcctacca 4261 attgcaaaaa ggcggtcatc gttccgaagg tgatacagcc tgcgctgagg tgaacggctc 4321 cccaaagaaa agccgtgagg tatccgccgg aaaaggcaag ggccactaac atgtgggcag 4381 aaagcgagaa acgggtacgt cccatgactt ggctgtggag catgtcttgc aagtcatcga 4441 gtttgtccag atgccgttcg ccttgttcca atgttttaat cactatgcga tgttgcaggc 4501 tctcttgtat gacggactgg atgcgaccgt cactgttccg aatatcatgt gtatagcgtt 4561 tcattcgtcg catatagaaa cggctgcctg atagaaagac gggcaggatg gcaacaatca 4621 gccatggcag tgaagggtct aaaaagtaga agaatagaaa ggctgccagc agttgtatac 4681 tcgtgacgat gaatgccggg acagaagagg tgagcaatgt gacgatggcg gtggtgtctt 4741 gttctacccg gttcactacg tctccggtat ggaaacgctc caattcgttc cagcggcttt 4801 gcagtaagcg cgagaagagg cggtggcgaa gggcgttgcc cgtctctatc tgcatacggg 4861 tacttatcca agtgtcagca gctccgcaca gtaattgggc agtcagcagg acaacggtga 4921 gaacggcgat atgcgtcagg ttgtcgtctg ttattccggt agcggtgtct atcacccgtt 4981 tggttgcata gataaaggct agggcaaagg ccacgcccag aatgccggtg aggcaactta 5041 gcaggatacg tccgcgttgt tcggcagata cctctcgaat atacctaagt ttttcacgtc 5101 tgtccttttt cacttttttg tccttctgtt gctttttcat tccttttatt ttccgggttc 5161 ttttatttct ttgtccttgc tttcttgtct tttactcctt ttgatcggtt tatttgtata 5221 gctatcgttc cccctatcag agtcacggga taccagagtg cttcgtacgg tgcaaattga 5281 cgcagttcgt tgcagcgtcg ggtggcgcgg cagaaggtgt accactttcc tgcccaatat 5341 cctttggggc gtggcttgat gcggttatca tgttgcccga agttaccggt agacaggatg 5401 tcttccaata attgttctcc tatctgcttg gtgccttcca aggggaaagg aagccggttg 5461 gcgggcaatc ccagtcgggt gacggcaatg tagccgaatg cttgtgcggc atgcaatagt 5521 ccgaggtcct gcaactggcg aagcagggtg gtggcatcta tttctttgtg gcgattggca 5581 agcagacaag tccagtcgca tagctggcgc aggccgattc cgctgttcag gaaatgtaca 5641 aaggcatgca ggaagatata aagtgcattg aagctggccg gaggtatggc gatactgacg 5701 gccttcgagt cctctttccc ggtttcggag aatagggcat agtctgtttc ctgcgggtac 5761 cattttctga taatctgctg aaagtaccgg ttggcaagcg gactgttcag ccggaggatg 5821 atgcggtggt tctcgatgtg tactcctttc aaactgtagc tggcatgctt gtcggacgct 5881 tctccctcga ctatggcgcc ttgttgcagc aggaggcggt tggctatggg ttgcccttgt 5941 tttcctaaat agatgtcgat gtctccgcat tgccggtgca agggagtgcg gtagttggtg 6001 ccgatgcctt gccctttcag cagtacggga tgcagcccgg cttcccggta aagggtattg 6061 agttcgggca gaagctggtt gaggcgtttg ttggcttgtt ctatctgtgc agtccgggca 6121 gcccatttca gatagaggac gcgtggaggg cgcagttcgg ccggcagact gcttatgccg 6181 tcaaaagtca gtgcaagcag ggcttgtgag tttgccatgc ggtacacggt ttcccagtcg 6241 tccggcgaga ccgaccggaa gagttgtcgg tctgccggag tgccccacag gccggagcgt 6301 accaatgaaa agaaagcgtg ttgcatatcc gtccttattc tatgattcct tcgcggtcaa 6361 gggagtcgat aaacttctgc gcatcgcgac gggccgattc gttatctaca tcatagcggc 6421 tggtcagggc ttctgtcacg ctgtctgtat cgaactcttt gttttctatc tcttgccaaa 6481 gcatggcgga agtttggttc aggctgatga ctttgctgaa gtccacgttc gacgagccgt 6541 cttgcatgat gatgtagtca tcgcctatcc ggtgcaggat aaatccttgt ttgattttca 6601 tggttatttt gttttatatt cttctgaaca gggcgagcag ccagcggcga gcggggttta 6661 gcttcatcca ccagtaggag tagagcttcc agactttgcc gtcggcggtg taccgtttgt 6721 cttttcgcat ggcttcggtc atgacgccca ttacccgtcc gatgtcggtt tcttcggtct 6781 gggcgaggtt gccgtcgcct tgcaggatga gtatgtctcc gttccgacgg atgatgcggt 6841 gaaagacgat gcgttccgtc aggtcgcgtg ccagtacggc gacgcccggt tgcagctgtt 6901 ctgtggtgaa atcgcccagt gtcacccggt cacggcggtc cactaagaaa ggattcatgc 6961 tgttgccccg cacggtgatg gttactgtat gcccctcttt gatgagacgc gccacttcgg 7021 gaagcaatat ctcgttggga atgcttttct tcatcggctg ttcagttcat cattcataac 7081 ttatccttct accgtctttc tgcaaagctc cgtcgcttca cggtcgggca ggcatttcag 7141 atgatagacg ggtgccagcg tggcaagttc cgtcacagtg gtgacaatgg cgttatagat 7201 ttccttgttc tgcttcaggc aagagcagga ggggaggagg gtggcgaaag catgtgctgc 7261 tctctcttgc tctatttcat tctgcggtgc ttgttccaag cggacgaagg cgcctacggt 7321 catggattct ttccgatagc atggtgtctt tccgctccac ggagaaccga aaacagtggc 7381 ttgcttgccg aggctgtcca catgtactat cggattgtca tcgttcagta gatggcagtc 7441 cgagaactgt tgcagccaga gcccggtgtg cgtacttttg cccgtgccgc ttttccctaa 7501 gaagaggtag cctttgcctg tttcagtagc gacaaccgag gcatgcatca gcagtgtatg 7561 gtggcgggcg gcattgaagg catagagcat catcaagaag ttgttcagta cgaagttcgt 7621 ttcgttttcc ggagccggtg tttctgtcct gcaggccggt aggtggatga agcattgcct 7681 gaagttatcg ttgcactcca tccaatagtt tttcccgctt ttccgtgggg tgatggatac 7741 aaggtgtgag ctgtggggca aagggcgaat gatgcaatcg gcatcttccc agttgaaaca 7801 tatatcatcg ggagttttcg ctatggttcg ttttgtcgct gtttcgtcat cgtctatctc 7861 tgtttcaaag attacttttt gcgtcgtgtc tattgtcgtg atgaacggaa tcagtgaagg 7921 caacgccttg aatatattga agtcggaagg tttcaccatg cggaaggtgt gcggtcctac 7981 ttgataatcc aatatctgta ttctcatatg ttttgattgt tagttgttat tcatgatact 8041 ttgggctact cagaaaaata atgaatgttt gtgtgaatga ggaagatttg agagattttt 8101 caactgtctt gttgtcattt tttagacgcg gaggacgcag atttataaaa gaaaatccgc 8161 tgtccgccgc atctaaaaga tagtaataag gcgattccga tacctcttcg tttggtctgc 8221 gaggatatat tgcttccttt cttagaattt ggcttatcgg ttctcctgac ttgttgggcc 8281 aacaatatat tctttcctcc catcgttgac tttgtgggct gctgtatata cactgccgtc 8341 acgggtaacc tcgtaacctg ttaccaaata ggattcattg tcgtccatat tcggatttgc 8401 cgggttgaaa ttgatgaacg ttccaccgga cacctggatg ctggcagtgt tcttgtctgt 8461 attctttaag ttcagtaccc agtatctgcc atccgtgtcg ttgttgggtg taccatattt 8521 gccggattca aatgttcctc catagatatt gattttccca ttctctcttg cataaatcag 8581 gtctatctgg gtgttcagtt tctgggtgtt atagtaagaa ccgccatgaa tatttactgt 8641 cccgtgtcct cgggcttcaa ttaccatgcg ataaccatcg tctttcgctg tagcagatgc 8701 atcgcacttc acattgccgt tgcctgaaat gtttagtgtt ccgttctgta cagtaaagat 8761 ttgtgtgaaa ggagtggcat cagctgtttc tgaggaattg atgatgtcgt ggccattcag 8821 gtttaagttt gccgttgcgt ttgcagcatt caatatgtag cctgtggcaa gctccgtctt 8881 ggcgatgttg atatttgaga gaagttttat atccacagtt gcgttggttg ccatgtactt 8941 cagtacgctt gggtcggaga atgtacctcc ggaaacagag atgatacttt tggcattttc 9001 ggttcccacg gatgcatcta cctttaagtc accgttgaaa gtaccgcctg tgatattcag 9061 cttcataggt tcggtatttc ctgcactctt ggaaatttcc acttttccat tgatgacgct 9121 cgtaccgctt accgtaaccg tgactccagc agtgtacttt gcatagccgc atacgtcgaa 9181 tgcaaattga ttcgcattgc ttcctgcgtt gatagtggta ttgttgatgc ttatattgcc 9241 acaattgttg cttactacgt attgggcatt tgtgtcttct actgtcatat tatctaatgt 9301 caggttgcag tagttctgaa tcatgatttt gttgttgtca ctcttcagcg ttccgttctt 9361 gaaagtaacg tttgaacctt cgagtaattg acagctgttc gtttcggtgc cggtggaacc 9421 tacggtcggg tcagcaagtg taagaatctt gccgcctaag tccatcgtca aggtttgtcc 9481 gcttgtggtc ttgaatcccg ggcaggtctt gtcggcggtc aactttatgt tcacatttgc 9541 atttgccttc atgtacttca gcgcgcttgg gtcggagaat gtaccgccgg taacgttgat 9601 gaccggtgta taagtggtgc cttctgtgat gagtgacttt gcttcggcaa tcagtgtacc 9661 acctttgatg tttactgtgg caatgccata tgctccactt acgttgatgg cggcgcagtc 9721 cagtcctccg gtaccgtcgc cccagttgcc ggtgctgcct gtgcctttac tggtaatcaa 9781 ggctgtgcct tccacgttca gagtgcctcg ttggatgcat acacctcctg cggcaccata 9841 aaccttgccg ccactgatgg tcgttgtgcc ggattggggc agatagactg catagtcagc 9901 agtagaaatg agttctccgt ctgtgatgtt gatgatggag ttctgcgtct tgtaattgcc 9961 gttgcctgcc actgcaaacc atccggcttc tatcttgccg cctgtcatgg tgaagtctcc 10021 accgtccgtc actccgacgc catattggcc gttactgttc ggagtctttc tgactgcact 10081 gatgttgccg ctttccatgg tcatggaggc atcttcaccg gctatttcaa tcagactgcc 10141 attataagaa gccgctgtat aatcctggct ggcaactatc ttgcctttct tctcggtaga 10201 gctgtccttc agtgtcatct tgcccaagac gatgattttg cctgttgcgc tgttgtcggc 10261 agtcagtgta tatccgttca agtcaagaac cacggacttt ccggcgggta cactgacgct 10321 ttgccctgtg atgtcggcat cggcggacaa tttaaaatct tcgccgttgg cgaaagcggc 10381 ctttaggact gccacgtcgg attgaactac aaatttgctg tcgattgttg ccggaagtga 10441 tgcttcggtt ccttttatta tatagattgt ggtatcgccg gcatcgtgta ccaattgttc 10501 tacctttcca tatatcttca agttaccgcc ttttacggtc agcttcttta cggtgacgcc 10561 tgcattgata atcaatgtct gctgggcggt agtggcagtc acttcattgt aagtggcttt 10621 attgcctact gctgccaatg taacggtggt gcttggcatg gtgatggcta cagaaggagc 10681 ttcttgctct gcattggttt ccggcacagc caaggataca ctgttggtgg cagtggcggc 10741 gggagcacct atgctggtat tggtcttgtc ggtcagcgtc aacggagcgg cagtactggt 10801 cgttggggct gtattgaacg ccaggttgat gtccgagttc tgtaaaaccg ggatggcgat 10861 gccgtcgttg ctgccggaag tggtattgaa gctgccgctg actgcaatgt cggttacggt 10921 tgctgctgtc ggtgcttctt gtgggagatt ttttgaatca gctaaagctg tactaagcgc 10981 actgggggtt gtagcctcta ccacaacgca atccaaagga ggtactacca atatgtctct 11041 tctgcctagg attttgtcat taagcgtctt ggtaaaatat accttgtcgc ttccattcgt 11101 cagttgggca gtgatggaac tataagtacc tgtgggcaaa ggaatgtaga agttgcgaga 11161 ctttatatca gctgaggctg tgaaggaggc ggtgatttcc ttgctttcat cgctcgttac 11221 tgccagtatc gggtctgctg cggttaagtc taccgtacaa gtacctgcaa tgttattgga 11281 ggcaataatc ttgaaagtag tggcctctgc cggaatcttg ttgacggtca gttttatcat 11341 ggctgccatg tgcttgaagg acagagcgct cagattgtcg ggatttgtca ccttggcata 11401 catcgggcca ttgctgctgc cgttataatt tgtaagcgtg gcaggtagag tcatggtcaa 11461 tgtattgccg cttacgctca tgttttgttg gtaaggatag atggagaata cagccgtttc 11521 tccgcttggc ttgctgccgt tgaaagtggc ggaagtttga ccagcaccgc ttgcatattc 11581 taattttgta ttgctataat tactttctgc attactacag aacaacccta gggcatcggt 11641 atcttgccat agaatcttgt attcatcgtt tacggtagta cgggtgtcgg tacctgcgcc 11701 ttcaaacgtt gcgttaacgg tgatgtttcc tttccgcccg ctgttttcaa attcctcttg 11761 gctgcaactg aatgcggtca aggcagcagt tgccatgcag gcaacttgca taaggtttct 11821 taatttcatc ataatgaagt cccccttagt tttctacggt taagatgtca ttgattaggt 11881 cttccaagtc gctgctggat gccgaattgt agcaatgggt agaggtgctg cccgaatagg 11941 agcgcgtaat gggatggtaa ggttcgccgc ttgttgcaat gactccttca ttctccattt 12001 tgataacttc gatagacgga gtgatgtatg cttctctacc gtctggtttg ttctgtttca 12061 tatacacaaa ttatttaaag gttaaacaat aaaaaaaaac gtgagtaact atgtttgtat 12121 atcctttatt caggccttcg cattgccctt tccctggata tacaacagtc actcacgtcg 12181 tttgtgtata taccatcccc ctgatgggag aaaaccatat aaacatagac gtgagcatcc 12241 ttgttgttat cttcagggaa attttcaaag ttgcgaagtt tgaataaaga agataatttc 12301 aataatgctc ttactaatat gtaaataaac ccactacgct atagcgaatt tgtgacaaag 12361 ataattgttt tttgtttaag tgtatctatg tttctctatt ttttttggga aaggctcacg 12421 tctctttcat ttataggagt taggagagtt atcacttcta actcccgata actccttcaa 12481 atatttagta actgtctgca ttgtctttga ggatgttttt ctgaggagca tagcggctat 12541 gtggcgaata aagagaggaa gacagaccga aaagaagctg aataaaatct ttttaagcaa 12601 aattaaaaca gagatttgat atactttaaa cagaacaccc tgcaaactcc ggcttactct 12661 gtccggcagt ttgcagggtg tctgcttggt tttctcacgt gtacgtagct ctttacgcca 12721 cgaacggaag tcgatgccat agcaattttt cataataaga ctttccatat ttttgcaaat 12781 gtccatactg cctttggctg aaacaattta tataagtaat tgaagtttcg taagtgagta 12841 tgcctgctga taaatgggaa tttatcgcac agagcgcgaa gcctgctctc ataacctatg 12901 gagagcacga gtgaaagctt gtggattttt atctaaaaca taggtattcc tttgctcttc 12961 tccctttgtt tgtctgctgt ggcataggga atttatgacg cctaacggcc atgaacaacc 13021 ctgctttcga ccataaagcg atcgcttgac gaccgtaagc agagttgttc atggccgtta 13081 ggcgtcacta actccagagc tattacggat gaagtacgta gcccgaacct attgcgcaca 13141 aggcgcaaac gtattataca acggtgtaaa agacattaga gtgcattatt catagtcggc 13201 ctttgattcc cgataactcc tactatgccc ctacagtcca actccatatc cgttgacttc 13261 acagtaagga caagcccgaa aatgcaggaa aaccgaacct gccgattcat gtaatctgca 13321 tgctgtatta tggtagggga tagcagctta ctgcaaaact caagcgtttc aggcaggtgg 13381 aagcaaattt tgcttccacc ttttcaggtt gcttccaccg cagggcaaat gccgatggac 13441 agggggatgc aggaggtggg tggaagtgtg gaagcaaagt gatggaaaat taatttggca 13501 ttcacacaat aataaccgta tatttgtagc atggctgtct gcctcctctt aaattcagat 13561 aaagaggagg tagacaaatg cttgctatga gaaacacata aatggttata accaagtgat 13621 gaaacaatta attgcatgtt gcggactgga ttgcgaaaat tgcgccgccc gtatagccac 13681 tgtcaataat gacgatgagc tgagagagaa aaccgccaaa gagtggagtg tgctgaacaa 13741 tacgccggaa attacggcag aaaccatcca ttgtatgggg tgccgtgcag acggggtgaa 13801 gtttgcctat tgcagcgatt attgtgctat ccgcaaatgt gtgtacgaaa aaggtttcaa 13861 tacttgcggt gactgtaagg aactggaaac ttgccaggtg gtaggtgccg ttcttcagca 13921 tgcacccggt gcaaaggaaa atctttatta accgtaaatt ctttgattaa ataaaatatt 13981 gcttatcttt gcagtatgac aaagacattt aaatcggatt tggttttgta aacaatctgt 14041 gttccttgca cagattgtcc ctttcgttta ctctcggata acttctttcg agagtgtttc 14101 tctattgtta taattaaagt ttaatccgcg tattatgctg aaggtattgt gatactttcc 14161 gtatatgcaa aatatagtaa gaaaatgagt aacgaaaata aaaccatcca cgatttcgaa 14221 cttagtttaa tctgtgactt tttctccaat atggaacggc aaggccctgg cagtcccgaa 14281 gcgacgttga aggcattgag ctttgtagac ggccttactg ataaatccct tattgccgat 14341 ataggttgtg gaacgggtgg ccagacaata gtagtagccg ggcatgtgcc ggggcggatt 14401 accggaattg accttttccc cggctttatt gacatcttca accgtaatgc aaggcagttg 14461 ggcttgcagg acagagtaaa aggaattgtc ggttcaatgg atgccctccc tttcggtgag 14521 gaggagctgg atatgatttg gtgtgaaggt gctatctata acatcggctt tgaacgtggc 14581 ttgaaggagt ggcgtaaata tctgaaaccg ggagggtata ttgccgtatc cgagagttca 14641 tggtttacgg acgaacgtcc tgcggaaatc aatgacttct ggatggacgc ctattctgaa 14701 atggatacac ttcccaatca ggtagccaag ttgtacaagg cgggttatct ccccgttgct 14761 acattcattc tgccggagaa ttgctggaca gagcattact ttgcttcaaa ggttgcggct 14821 caggagattt tccgtaagaa atatgccggt aataaaattg ccgaagagtt cagctcactc 14881 caaatgattg aggatgaact atacggcaaa tataaggaat tctacggtta tgtgtttttt 14941 attgctaaga aggtagattg atggcagact tctcttattg aggaatgcgg gtattctcat 15001 ggcagaatat ccgcattttg tgttttgagt ggattttatt gttttgagga tgtgcggaac 15061 ttctgaggag gtattcccgt atgacgttta aagaatttgt tgaagaaggc cgcattatta 15121 aaacctagac tcacggctat atcctttatc ggagtgttgg cggtacaaag ttgtgctttg 15181 gcatccatga tgatagtatt gttgatgatt tccatggctg tatgtcctga ttcctttttg 15241 acaaggcggc aaagagctgc cggtctcata ttcattttca atgcataata ggacacttga 15301 tgctcccgga catagtctgt gtgtaccaat cggataaaaa tgcggtattg gttgaaatgc 15361 ctgctgcccg gagtctcttt tattctaggg cgttccggac agagttcgga tataccttgt 15421 aacagatagg tcagtagagt ttgcaatgaa actagacttg aggggctttc tgttctttgg 15481 tagaggtgtg acaaataggt catgaactcg gcataaagct ggaagtccgt tttcgataga 15541 ggaaaaatat agtgtttgcc tataatgtga aacttatcca tcatgacctt gccggcccct 15601 gaactctgga taagttgttg ggaaaatatg acgacatata tttcagcatc gtctgacgtg 15661 tgtagcaatt gtacgaaggt ctcgggagga ataactagga gggtgttagc ttcaaccctg 15721 aattgagtat ggtttatgat ggttttcaga actcctttgg tgcatagagc gaagatactg 15781 gccttggtgt ggtatggctt ttccactagc ggattcagtt cattggttgt gatatgattg 15841 caggcaatga actcggcatt tatattgaat tcggatatat tcatgttgga gattttaaga 15901 cacaaaaatg ggggataata atgtataaat ga //