LOCUS       c00242_gnlPro..         5706 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PDHDCJFA_242.
ACCESSION   c00242_gnlPro..
VERSION     c00242_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:24:45
            Original ID  :: gnl|Prokka|PDHDCJFA_242
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5706
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5706
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1713:5704]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1714..5704
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5706
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5706
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             2..1585
                     /locus_tag="ctg242_1"
                     /transl_table=11
                     /translation="AEYDPKFIDLQTYITYQLAPKWEINFLGNLANNNYKFTPYSRETS
                     FGTAEHPKNFKVYFDGRERDRFQTLFGALTLKHNPNENTELGLQASAFKSKEEEGYDIA
                     GEYWLSENGAENPNDDASAAMSVGRYHEHARNRLNSTVMNVGHYGMARLLNNTLKWGAT
                     VQMEKINDKISEWEKRDSSGYSLPQTGNNVSVYSNLFSDNQIESTRFSAYAQDAFKFRT
                     KQGLFTLVAGVRGSYWTYNKEFLFSPRASLGFIPNFDQDLTLRFATGLYYQSPFYKELR
                     RVDKDKNGNNITVLNKDLKSQRSIHFILGGDYTFRAVDRNFKVTAEMYYKKLDNLNPYT
                     VDNVKIRYYGENCAKGYAMGLDVKFFGEFVPGTDSWISFSLMKAQQTIRETTTVPMANS
                     QGYNISLFFQDYFPGYKRVKLNLKGVLSGGLPQTIPGKGYEAGYFRTPAYKRVDIGLSY
                     QLAGGTDAIMDRGFFRHLKNIWLGLDVFNILDIKNVSSYYWITDVYNVQYAVPNYLTGR
                     QLNVRLIVDF"
     CDS             1714..2124
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg242_2"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-27, bitscore:
                     85.0, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEGVLGLKCYNIEVVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALNEAEEKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGNK"
     CDS             2161..3714
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg242_3"
                     /sec_met_domain="CT_Btheta (E-value: 5e-293, bitscore:
                     960.5, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALREQARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIFAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVVPQI
                     SGIFGPCAGGAVYSPALTDFNIMTRGTSYMFLTGPKVVKTVTGEDVTQEQLGGASVHTT
                     KSGVAHFAVDTEEDGLQLIRKLLSFLPQNNLEETPLVECKDPIDRLDDVLNDIIPDNAN
                     QSYDMYEVIGTIVDNNEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKVMAGCLDSNA
                     SRKAGRFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKITV
                     TLRKSYGGAYCVMSSKHLRGDMNYAWPTAEIAVMGPSGAVEVIFAKEVAAAEDPKACAA
                     EKEAEYKKAFANPYNAAQYGYIDDVIEPRNTRFRIIRALQQLQTKKLSNPAKKHDNLPL
                     "
     CDS             3736..4623
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg242_4"
                     /sec_met_domain="OAD_gamma (E-value: 1.4e-13, bitscore:
                     40.1, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINKKIGVLLLFALLISFGAKAQRATSMRINEVLVINEDNFVDDY
                     GKRHGWIELFNTSAGTVNIAGCYLTDDKNNPMKYPIPKGDVLTQIQPRQHTLFWADGQP
                     GRGTFHVNFVLDPSKENYIALYDADGKTLIDEVTIPAGQMADISYGRVIDGKDEWAQLK
                     KVTPSTNNLTLDSNEKIDNFKQNDSLGIGMTITAMAVVFLGLFLLYIIFKQIGRLSISA
                     SKRNAQKAAGTTSVSVDAGQESGEIFAAIATALYEMSDDNHDIEHTVLTIHKVQRTYSP
                     WSSKLYGLRQTPHK"
     CDS             4645..5076
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 64.8; E-value:
                     1.6e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg242_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.5e-21, bitscore:
                     64.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSFKYTINGNVYKVHINSVVDDIADVEVNGTPYQVKLEKPAKKQ
                     MVTLKRPAQAPTTASGDPVVTRPAASTTQGAVKTPLPGVILQVKCNVGDTVKRGQTLII
                     LEAMKMENNINADRDGKIIEIKVHKGDSVLEGADLVVIG"
     CDS             5081..5704
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg242_6"
                     /sec_met_domain="OAD_beta (E-value: 1.9e-78, bitscore:
                     252.5, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESFLTFLGENLQLFLTYTGVYNATPGHLIMILVGLLFIFLAIK
                     YEFEPMLLIPIGFGILIGNIPFKDAGLQVGIYEQGSVLNILYQGVTSGWYPPLIFLGIG
                     AMTDFSALISNPKLMLIGAAAQFGIFGAYTIALQMGFEPNQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPIIQPPFMRLLTTKKERLIRMK"
ORIGIN
        1 cgcagaatac gaccctaaat ttatcgatct gcaaacttac atcacgtacc aattggcccc
       61 taaatgggaa atcaatttct tgggaaacct cgctaacaat aattataaat tcacccctta
      121 tagccgggaa acctctttcg gtacagccga gcatcccaag aacttcaaag tctatttcga
      181 cggaagggaa agagaccgtt tccaaacctt attcggtgcg cttacattga aacacaaccc
      241 caatgagaac acagagttgg gacttcaagc ctccgctttc aaaagcaagg aagaagaggg
      301 atacgatatc gccggagaat attggctgag cgagaacgga gcggaaaatc cgaatgacga
      361 cgcgtcagcc gccatgtccg taggccgtta tcacgagcac gcacgaaacc gtttgaactc
      421 caccgtcatg aatgtcgggc actatgggat ggcccgcctt ttaaacaata cgttgaaatg
      481 gggagccacg gtacaaatgg agaaaatcaa tgataagatc agtgaatggg aaaaaaggga
      541 ttcctcaggt tattctttgc cccaaaccgg taataacgta agtgtatatt cgaacctctt
      601 ttctgataat cagatagaaa gcacacgctt ttccgcttat gcgcaagacg cattcaaatt
      661 caggaccaag caaggtctgt tcacgttagt cgcaggagtt agaggtagtt attggactta
      721 taataaggag ttccttttca gtccccgtgc ctcattgggc tttatcccga acttcgacca
      781 agacttgacg ctccgtttcg ccaccggact gtattatcaa tcgcccttct acaaggaatt
      841 acgtagagta gacaaagata agaacggcaa taatatcacg gtattgaaca aagatctaaa
      901 gtcacaacgc tccatccatt tcatattggg aggagattat acgttccgtg ccgtagaccg
      961 gaatttcaag gtgaccgccg agatgtacta taaaaagctg gacaatctga atccctacac
     1021 cgtagataac gtcaagatcc gctattacgg tgagaactgc gccaaaggtt acgccatggg
     1081 actcgatgtc aaattcttcg gagaattcgt accgggtacc gactcatgga tcagcttctc
     1141 cttgatgaaa gcccagcaaa ccatccggga aacaacgacc gttcctatgg ccaatagcca
     1201 aggctacaac atctcactct tcttccaaga ttatttcccg ggctataaac gagtcaagct
     1261 aaatctgaaa ggagttctat ccggaggact tccccagaca atcccgggca aaggatacga
     1321 agccggctat ttccgtacgc ccgcctacaa acgtgtagat atcggcctct cctatcaatt
     1381 ggcaggaggc acggacgcta tcatggacag gggcttcttc cgccatttga aaaacatttg
     1441 gctaggtctg gatgtattta acatattaga catcaaaaac gttagttcct actattggat
     1501 aacagatgta tataatgtcc aatacgcagt tccaaactat ctgaccggac gtcaattgaa
     1561 cgttcgtctg attgtcgatt tttaataaaa tgtatcttta aacacattta ctgggagata
     1621 aaaacaaaaa attatttcct cattcataaa aagtagtgta catttgcaca caatagaaac
     1681 aaacatttac taaatcttta aattattatt gacatggaac ttacacatat cgaacattta
     1741 ggtatcgcag tgaagagtat cgaggcttgt ttaccctatt atgaaggcgt tcttggatta
     1801 aaatgctaca atatcgaagt agtagcagac caaaaagtaa aaaccgcatt tttcaaggta
     1861 ggacagacta agattgaatt actagagccg accagcgaag atagcacgat cgcaaaattt
     1921 atcgagaaaa aaggtgaagg tatccaccat atcgcattcg cggtacctga tgtacaggca
     1981 gctttgaacg aggctgagga aaaaggcgtt aaattaatcg ataaagctcc ccgtggtggc
     2041 gcggaaggct tgaacatcgc tttccttcac ccgaaatcaa cttgcagcgt attgacagag
     2101 ctttgcatgc cgggcaacaa ataattaatc cgttcatttc taacaacaaa tcaaaataca
     2161 atgagtaacc agcttgaaaa agtaaaagag ctcatcgcac ttcgtgagca agctcgctta
     2221 ggtggaggag agaaaagaat cgaatcacag cacaaaaaag gcaaatatac cgctcgtgag
     2281 cgtatcgcca tgttgcttga cgaaggtagt ttcgaggaat tcgacatgtt tgttcaacac
     2341 cgttgtacaa acttcggtat cgagaaaact aaatatttag gcgatggtat cgtaacaggt
     2401 tatggtacga tcgacggacg tttggtatat atcttcgctc aagatttcac cgtattcggt
     2461 ggagccttgt ctgaggcttt ggctatgaag atctgtaaag taatggatca agccatgaag
     2521 atgggcgctc ccgttatcgg tttgaacgac tccggtggag ctcgtatcca agaaggagta
     2581 aatgccttgg caggatacgc tgagattttc caacgtaaca tcttggcttc cggtgttgtt
     2641 cctcagattt ccggtatctt cggcccttgc gccggtggag ccgtttattc tccggctttg
     2701 acagacttca acatcatgac acgtggtacc agctacatgt tcttgacagg tccgaaagta
     2761 gtaaaaaccg taacaggtga ggatgtaacc caagagcagt tgggtggagc cagcgtacac
     2821 actaccaaat cgggtgtagc ccatttcgct gtcgatacag aagaagatgg tttgcaattg
     2881 attcgtaaat tgctcagttt cttacctcag aacaacttag aggaaacgcc gttagttgag
     2941 tgcaaagacc cgatcgatcg tctggatgat gtattgaacg atatcattcc cgataacgcc
     3001 aaccaatctt acgatatgta tgaggttatc ggtacaatcg tcgataacaa cgagttccta
     3061 gaggtccatg cggactacgc taagaatatc atcgtaggtt tcgctcgttt caacggacaa
     3121 tccgtcggta tcgtagctaa ccagccgaag gttatggcag gatgcttgga tagcaacgca
     3181 tctcgtaaag caggtcgctt cgttcgtttc tgcgacgctt tcaatattcc tttggtaaca
     3241 ttggttgacg ttccgggatt ccttccgggt acaggtcagg aatacaacgg tgtaatcctt
     3301 cacggcgcta aattgcttta cgcttatggt gaggctaccg ttcctaagat taccgttaca
     3361 ttgcgtaagt cttacggtgg agcttattgc gtgatgagtt ctaaacattt acgtggtgat
     3421 atgaattacg catggccgac agccgagatc gccgttatgg gcccgagcgg tgccgtagag
     3481 gttatcttcg ccaaggaagt agcagccgct gaagatccga aagcatgcgc agcagagaaa
     3541 gaagctgaat ataagaaagc attcgctaat ccttataacg cagctcaata cggttatatc
     3601 gacgatgtta tcgaaccgcg taacactcgt ttccgtataa ttcgtgcttt gcagcaattg
     3661 cagactaaga agctaagcaa tcctgctaag aaacatgata acctgcctct ataatccgta
     3721 aaacaggaga tgaatatgat aaataagaaa ataggggtat tgctactgtt cgctttgctc
     3781 atttcattcg gggcaaaggc acagcgcgca acctccatgc gtattaatga agtattggtt
     3841 atcaacgagg ataacttcgt tgacgactat ggcaaacgtc atggctggat tgagttgttc
     3901 aacacttcgg ccggaacggt aaatatcgcg ggatgttatt tgacagacga caaaaacaat
     3961 ccgatgaaat atccgatccc gaagggagac gtattgactc agatccaacc ccgccagcac
     4021 acactatttt gggctgacgg acagccgggc agaggtacat tccacgttaa cttcgtatta
     4081 gatccctcta aggagaatta tatagctctg tatgatgcag atggtaaaac attgatcgac
     4141 gaagttacga tccctgccgg acaaatggct gatatcagtt acggtagagt gatcgatggt
     4201 aaagatgaat gggcacagct gaagaaagtt actcccagta cgaataacct aacattggat
     4261 tctaatgaga aaatcgataa tttcaaacag aatgactctt taggtatcgg tatgaccatt
     4321 accgctatgg ctgtagtgtt cttgggatta tttttgttat acatcatctt taagcagatc
     4381 ggtagattgt ctatatccgc gagcaaacgt aacgctcaaa aagctgcggg aacaacaagt
     4441 gtatcggttg acgccggaca agagtctggt gagatctttg ccgctatcgc taccgctttg
     4501 tacgagatga gtgatgacaa tcatgacatc gagcacacgg ttcttacgat ccacaaggta
     4561 caaaggactt attctccttg gagttcaaaa ctctacggtt tgcgtcaaac cccacataag
     4621 taattatcta aaaacgaaca aagaatgaaa agttttaaat ataccattaa cggtaacgta
     4681 tataaagtac acatcaattc tgtagtggat gacatcgcag atgttgaagt aaatggtaca
     4741 ccttatcaag taaaattaga gaaaccggcg aagaaacaaa tggttacatt gaaacgtccg
     4801 gctcaagcac cgacaacagc aagtggcgac cctgtagtta ctcgtccggc agcctctact
     4861 acacaaggtg ctgttaagac tccgctgcca ggtgttatct tgcaggttaa gtgcaacgta
     4921 ggtgataccg tgaagagagg tcaaacattg attatccttg aggctatgaa gatggagaac
     4981 aacatcaacg cagaccgtga cggtaagatt atagaaatta aagtacacaa gggagactct
     5041 gtgctagaag gtgctgatct cgtagtaatc ggataagact atgcaagaga gctttttaac
     5101 attcttaggt gagaacttac aactgttcct tacctataca ggtgtataca acgctactcc
     5161 gggacatctc atcatgattc tggtaggtct gttgtttata ttcctggcga ttaaatatga
     5221 gtttgaaccg atgcttttga tccctatcgg atttggtatc ttgatcggta atatcccttt
     5281 caaggacgca ggtcttcagg taggtattta tgagcaaggg tctgtactga acatcctcta
     5341 tcagggtgtg acctctggat ggtatccgcc attgatcttc ctcggtatcg gggccatgac
     5401 cgacttctcc gcattgatct ccaatcctaa gttgatgttg atcggtgcag cggctcagtt
     5461 cggtatcttt ggtgcttata cgattgctct tcaaatggga tttgagccga accaagcagg
     5521 tgctatcggt attatcggtg gtgctgacgg tccgacggct atcttccttt cttctaagtt
     5581 agcgccaaac ttaatgggtg cgatcgcggt atccgcttac tcatacatgg cgttggttcc
     5641 tattatccaa ccgccgttca tgagattgct tacgactaag aaagagcgtt tgatccgtat
     5701 gaaacc
//
