LOCUS       c00416_gnlPro..         7298 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PDHDCJFA_416.
ACCESSION   c00416_gnlPro..
VERSION     c00416_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:24:45
            Original ID  :: gnl|Prokka|PDHDCJFA_416
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7298
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7298
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2399]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2399
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7298
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7298
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..1664
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg416_1"
                     /sec_met_domain="Gly_radical (E-value: 2e-46, bitscore:
                     146.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.8e-200, bitscore:
                     654.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RFLNRLDIQEITEEIIQSREEISEQIKALKAFEKMCASYGFDVTR
                     PAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEKDIREGKLTEEEAQELV
                     DQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSLVTRTSYRYLHTLYNLG
                     PAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRPDYGDDYGIACCVSPMK
                     IGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRGEYLEYDEVMAKYEQMM
                     RWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIVADSLAAIRDT
                     KVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQHETYRHAKPTQ
                     SILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALASVAKLPFQHAH
                     DGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNVNVFDKDLLIDAMEHPE
                     KYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             1665..2399
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg416_2"
                     /sec_met_domain="Radical_SAM (E-value: 1e-33, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             2598..3176
                     /locus_tag="ctg416_3"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             3284..3913
                     /locus_tag="ctg416_4"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGEN
                     EALELRDGDKKRYGGKGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSK
                     LGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQE
                     FMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSSRRLVMNRARM"
     CDS             3913..4485
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MAAL_C"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg416_5"
                     /sec_met_domain="MAAL_C (E-value: 2.4e-08, bitscore: 22.5,
                     seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGMDCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINE
                     YPIDSIEDGMSENDWAGWKKLTDRIGNHCQLVGDDLFVTNVEFLSRGIKEGCGNSILIK
                     VNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSD
                     RMAKYNQLLRIEEELGDRAVYGYKRIN"
     CDS             4706..5272
                     /locus_tag="ctg416_6"
                     /transl_table=11
                     /translation="MEPIINSQLPEFKVQAFQNGNFKTVTNEDVLGKWAIFFFYPADFT
                     FVCPTELVDMAEKYEQFKSMGVEIYSVSTDSHFVHKAWHDASETIRKIQYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKVAEIHDNNIGRDASELLRKVEAAQFVASHD
                     GEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             5292..6845
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg416_7"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.1e-49, bitscore:
                     156.8, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VLESTLKEQLKGIFAGLNANYTFDISISPAHESRQELLDLLGDVA
                     SCSDKISMQVSDGEGLEFILLKDGAKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNFPD
                     ESICNRVKALNGPIHLTTYVSLTCTNCPDVVQALNAMTTLNPQIHHEMVDGAINQAEVD
                     ALKIQGVPSVFADGKLIHVGRGEFGELLSKLEAQYGINESLTEKTVKRYDVVVVGGGPA
                     GASAAIYSARKGLSVAVVAERIGGQVKETVGIENLISVPETTGTQLADNLRLHMRQYPI
                     DLLEHRRIEKVTIEGNEKVLSTAGGEIFKAPAVIIATGASWRKLNVPGEAEYIGRGVAF
                     CPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEKAKSL
                     PNVEIFVSSQTTEVVGNGDKVTGIRTKDRKTGEERVIHLDGIFVQIGLAANSGVFKEIV
                     ETNRPGEIVIDAHCRTNVPGIYAAGDVSTVPFKQIIISMGEGAKAALSAFEDRVRGVLG
                     "
     CDS             6930..7280
                     /locus_tag="ctg416_8"
                     /transl_table=11
                     /translation="MDRLRKYLRILLPALFIAYLGCIIAFTHVHIVNGVTIVHSHPYHK
                     TDDGRPDHEHGYAEFQLLHQLSVLQITGAAFVAILLAAFYSFIRNLSFAPVYPDYIVPF
                     RGRRTLRAPPIF"
ORIGIN
        1 agcgtttctt gaatcgtttg gatatccaag agatcacgga agagatcatc cagagccgtg
       61 aggagatttc cgaacagatc aaggctttga aagcgttcga gaaaatgtgc gctagctatg
      121 gctttgatgt gactcgtccg gcacaaaacg ctcgtgaggc ggttcagttt gtgtacttag
      181 cttatctggc ggcggtaaaa gaccaagatg gcgcggcgat gtctatcgga cgtacttcta
      241 cgtttttgga tatttatatc gagaaagaca tccgtgaggg taagttgacc gaagaggagg
      301 cgcaagaatt ggtagaccag ctgatcatca agttgcgtat cgttcgtttc ttacgtacgc
      361 cggaatacaa tgatttgttc tccggtgatc cggtttgggt aacagagtcc ttgggcggcc
      421 aaggagtgga tggccgttct ttggtaacaa gaacttccta tcgttatctc catacattat
      481 ataatctagg tccggcacca gaacccaatt tgacagttct gtggtttaag aacgctcccg
      541 agaactggaa acgcttctgt gccaaggtct ctatcgatac atcggctatc caatatgaga
      601 acgacgattt gatgcgtccg gattatggag atgattatgg tatcgcttgt tgcgtttctc
      661 ctatgaagat cggtaagcag atgcagttct tcggagcccg tgcgaatttg gcgaaatgtt
      721 tattatatgc gatcaacgga ggacgggatg agcgcagcgg cgtacaggta gccccgatgt
      781 tcgagccggt tcgcggcgag tatctggaat acgatgaggt gatggctaag tatgagcaaa
      841 tgatgcgctg gttggcgaaa gtgtatgtga acgccttgaa gataatccat tatatgcatg
      901 ataaatacgc atatgaggca ttcgagatgt cgcttcacga tggagatgta gagcgtatcc
      961 gtgcgacagg tatcgccggt ctttctatcg ttgccgattc gttggctgct atccgtgata
     1021 cgaaagtacg tgtgatccgt gacgagcgtg gcttggcggt tgatttcgaa cgtgagggcg
     1081 aatatgttcc tttcggtaat aacgacgatc gtacggatag tatcgctgta gagatcaccg
     1141 agaagttcat ggaatatttg cgtcagcacg agacctatcg ccatgcgaaa cctacgcagt
     1201 ccatccttac gatcacctct aatgtggtat atggtaagaa gactggtacg acaccggatg
     1261 gccgtatcgg tggaactcct ttcgctccgg gcgcgaaccc tatgaacggt cgtgatacga
     1321 aaggtgctat cgctgcgcta gcttcggtag cgaagcttcc attccagcat gcgcacgacg
     1381 gtatctctta tactttcgct gtttctccgg ctacgttggg aaaggagcgt gaagtgcaga
     1441 tcaataatct ggttagctta ttggatggct attttactcc ggatggaggt cagcatctga
     1501 atgttaacgt atttgataag gatttgttga tcgacgctat ggaacatcct gagaaatatc
     1561 cgcaacttac gatccgtgta tccggatatg cggtaaactt cgtgaaactg actcgtgaac
     1621 aacagttgga tgttatttca agaaccatta accattcact gtaagtgata aaaggtaaga
     1681 tacattcatt agagagtttc gggaccgtag atggtcccgg aattcgcttt gtcgttttta
     1741 tgcagggctg cccgttgcgt tgcctgtatt gccataaccc ggatacttgg aacccgaaag
     1801 ggaaggtgaa atatcagatg actcccggcg agctattgac ggaagtctta cggtataaga
     1861 gttttatcgc ccgtgggggg gtgactgtta ccggtgggga acctttattg caacctgagt
     1921 ttctgaaaga gtttttccgt ttgtgccaag agcaaggtct tcacacagct ttggacacat
     1981 ctggtttcgt atgtacctca aaagcttggg aagtcttgga ttatgcggac ttagtgttat
     2041 tggacatcaa aaccttaaac ccggatttac atccgttact ggcgggtgtc aagcaggata
     2101 atacgcttct gttcttggat gaattggaga gacgtggcat agatacttgg attcgccatg
     2161 ttattgtccc cggatatacg gataatgacg agtggctgga ggcgttagct cggtatgtca
     2221 gttcatataa agtggttcgc aaagtggaat tattgcctta tcatacgatg ggaacctata
     2281 aatatgagca attaggtctc gattatccgt taaagggggt ggaaccctta tcaaaagaac
     2341 gtttagataa tgcgaaagcg atattctctc gctacaaacc agaaaataac aaggcctgat
     2401 ctctcgatca agccttgcga ttatgcaaca gctaatcagg gaaaacaatc taatccgatt
     2461 agcttttaaa cgatagtaat aattttttgc ctagactaac cttgactatt taatatgtgt
     2521 ttgttttgtt atatgcaaca aatatctatt tttgtaagga gataaaaaat gatatagatc
     2581 agaaaatgat gaatagtatg aagacaccac aagaaatagc cgaaatgata tccaaacgtt
     2641 tttatccatt acccgatgcg gatttggatt ctctggcctc gatcatagaa tcaagtaagg
     2701 ctgcgaaagg agaattattg ttggataacg gacaaatatc taaatattta tattatgtgg
     2761 aatcgggatt attacgccag ttttactata agaataaacg tgatattacc gagcatttct
     2821 cctgtgaggg aaatatcgtt atttgtatag ccagcacatt ccaaagagaa ccgacgcgtt
     2881 tattaatcga ggcattggaa ccgacggttt atcaccggat ctctttttcg gaatttagcg
     2941 atttattgaa aacatcttta gctattaata gattgtacca gaaaatactg gagtgggctt
     3001 tgatgctttc gcaagagaaa gcggactctt ggcgttttga gtcggcaaga aaacgttatc
     3061 tgcgttttca acaggaatat ccggaagcag cgaaacgtgc ttctgtcaat catatcgcct
     3121 cgtatttatt gatgacaccc gaatctttga gccgggtgcg ggcgggagtg ttgtagtctc
     3181 tacctacatt caggtattat tgaggtgaaa atagcaattt gtggtgattt cacggttcaa
     3241 gtaatccatc tatatttgcg tcagtttaaa aaacgtagaa gcaatgaaaa tagaaaagat
     3301 tacaggacgt gagatcctag attcaagagg taacccgaca gtagaggttg atattttact
     3361 agagtccggc gttatgggcc gtgcgtctgt gccttccggt gcgtccaccg gcgagaacga
     3421 ggctttggag ttgcgtgatg gcgataagaa gcgttatggt ggtaaaggcg tattgaaagc
     3481 ggttgagaat atcaatacga ttatcgctcc ggcattgaag gggatgtctg ccttggatca
     3541 gataggtatc gatcatgcga tgttggcttt ggacggtacg aaaactaaat cgaaactagg
     3601 tgcgaacgct atccttggcg tttctttagc tgtagcgaaa gctgccgcaa attatttgga
     3661 tattccttta tatagatata ttggtggaac gaatacctac gtgatgcctg ttccgatgat
     3721 gaatataatc aatggcggtt cgcatagcga cgctccgatc gctttccaag agtttatgat
     3781 tcgtccggta ggtgccgctt ctttcaagga aggcttacgt atgggcgctg aggtattcca
     3841 tgcgttgaaa aaggtattgc acgatcgtgg gttaagttca aggcggctgg ttatgaaccg
     3901 ggcaaggatg tgatgatcgg tatggattgc gcttcttctg agttttataa ggatggaatc
     3961 tacgattaca cgaagtttga aggagaaaaa ggagtgaaac gtacttccga cgaacaagtg
     4021 gattatctgg aaaagctgat caacgaatac ccgatcgatt ccattgagga tggtatgagt
     4081 gagaatgatt gggctggttg gaagaaactg acagatcgta ttggtaacca ttgccaatta
     4141 gtcggagatg atttgttcgt gacaaatgta gagttccttt caaggggtat taaagaaggt
     4201 tgcggtaact caatcttgat caaggtaaat cagatcggtt ccttgacgga gacactgaac
     4261 gctatcgaga tggctcaccg tcatggatac acgaccgtta cttcacaccg ttccggtgag
     4321 acggaagacg ctacgatcgc cgatatcgct gtcgctacca atagcggaca gatcaagacg
     4381 ggatcattaa gtcgttcgga ccgtatggct aaatataacc agctgcttcg tatcgaggag
     4441 gaattgggag accgtgccgt atacggatac aaaagaatca attaagtata taaagtaaat
     4501 tataatcgct aattatgtag taatccaaaa cccaagatat tgaagggctt atccgtgagg
     4561 ataggccctt tttattgatt gataaagtat tatctataac ctcataaaat atatcgatta
     4621 gaaaaattgt tttaatgaat aaatatgtta tatttttgta tcggaaataa aaacaaatag
     4681 tattcattta aaaataggaa caagtatgga accgattatt aattctcaac ttccggagtt
     4741 taaagtacaa gcttttcaaa atggtaattt caagactgta accaacgagg acgtcttggg
     4801 taagtgggct atctttttct tctacccggc cgattttaca tttgtttgtc caacagagtt
     4861 ggtcgatatg gctgagaagt atgagcagtt taaatcaatg ggagtagaaa tttactctgt
     4921 aagtacagac tctcattttg tacataaagc ttggcatgac gcttccgaga ctatccgtaa
     4981 gatccaatat ccgatgttgg cagatcccac tggagcactg agtcgtgcgt ttggtgtgat
     5041 gattgaggaa gagggtatgg cataccgtgg cactttcttg gtaaatccgg aaggaaagat
     5101 caaagtggcc gagattcatg ataacaatat cggtcgtgac gcttccgagt tacttcgtaa
     5161 agttgaagcc gctcagtttg tcgcttcaca tgatggtgag gtttgtcccg ctaaatggaa
     5221 gaaaggggag gctaccttga aacctagcat tgacttggta gggaaaatct aaataattaa
     5281 gagtatagat cgtgttagaa tcaacattaa aagaacaatt aaaaggtata ttcgcagggt
     5341 taaatgcgaa ttataccttt gatatatcga tctctcctgc gcatgaaagt agacaggaat
     5401 tgttggattt attaggtgat gttgcctctt gttcggataa aatatctatg caagtatctg
     5461 atggggaggg attggaattc attttattaa aggatggagc gaaaacgggg attaagtttc
     5521 gtggagtacc taatggacac gagtttactt ccttgctgtt ggcgatcttg aattcagatg
     5581 ggaaaggcaa gaattttccg gacgagagca tttgtaatcg tgttaaagcc ttgaatggcc
     5641 cgatacatct gacgacctat gtctctttga cttgtacgaa ttgcccggat gtggtacagg
     5701 cattaaacgc tatgacgact ttaaatcctc agatccatca tgagatggta gatggggcta
     5761 tcaatcaggc ggaagttgat gccttgaaga tacaaggcgt cccctctgtc tttgcggatg
     5821 gaaaactgat ccatgtagga cgtggggaat tcggtgaatt gttatcgaag ctggaagctc
     5881 agtatggaat caatgagagc ctgacggaga agacggtaaa acgatatgac gtggttgtgg
     5941 tcggtggggg tccggctggt gcttctgccg ctatttattc cgcacgaaag gggctgagtg
     6001 tcgctgtcgt ggcggagcgt atcggcgggc aggtgaagga gacggtagga attgagaatc
     6061 tgatttcagt tccggagaca acaggtaccc agttggctga caatctgcgc ttgcatatgc
     6121 ggcaatatcc gattgacctg ctggagcatc gtcgtataga gaaggtaacg atagaaggaa
     6181 acgagaaggt tttgtctacg gctggaggtg agatattcaa ggctccggcg gttattatcg
     6241 ctacgggcgc tagctggcgt aaattgaatg taccgggtga ggcagagtat atagggcgtg
     6301 gagtggcttt ttgtcctcat tgcgacggtc cgttttataa gggaaaacat gttgccgtgg
     6361 ttggcggtgg aaattccggt atcgaggcgg ctattgattt ggcggggatc tgttcgaaag
     6421 taaccgtttt ggagtttatg gatgagttaa aagcggatca agtcttgcaa gagaaagcga
     6481 agagtttgcc gaatgtcgag atctttgtta gttcacaaac aaccgaggtg gttggaaatg
     6541 gcgacaaggt aaccggtatc cgtacaaagg atcgtaagac aggggaagag cgtgttattc
     6601 atttagacgg tatttttgtt caaatcggct tagccgctaa tagtggtgtc tttaaggaga
     6661 ttgtcgaaac aaatcgtccc ggtgagatcg tgattgacgc acattgccgg acgaatgtcc
     6721 cgggtattta tgcagcgggc gacgtttcca ccgtaccctt taagcaaatc attatttcta
     6781 tgggagaagg cgccaaagcg gccctttcgg cctttgaaga tcgggtacgt ggtgtactcg
     6841 gttgatgaaa tacctacaaa taggtaattt ttgaacagcg tatctctttt atcggttata
     6901 ttactatatt tgcgatcgag agaaaagaga tggatagatt aagaaaatat ctgaggatat
     6961 tactccctgc gctgttcata gcctatttgg gatgtattat tgcgtttacc cacgttcata
     7021 ttgtcaacgg ggtaacaatc gtccattctc acccgtatca taaaacggac gatggtcgtc
     7081 cggaccatga acatggctac gccgagtttc agttgcttca tcagttatct gtcttgcaga
     7141 taacaggtgc ggctttcgtc gctattctgc tggcggcttt ttattccttt attcgtaact
     7201 tatctttcgc tccggtctat ccggactata tcgttccctt ccggggaaga cgtacgcttc
     7261 gggctcctcc catattctga tattgtgaga ttaataac
//
