LOCUS       c00250_gnlPro..         4252 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PKKOEMMK_250.
ACCESSION   c00250_gnlPro..
VERSION     c00250_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 09:42:45
            Original ID  :: gnl|Prokka|PKKOEMMK_250
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4252
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4252
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[65:4189]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      66..4189
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..4252
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4252
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             66..1901
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_1"
                     /sec_met_domain="ILVD_EDD (E-value: 3e-217, bitscore:
                     709.9, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNPLRSSYSTEGRRMAGARALWRANGLKEEQFGKPIIAIVNSFT
                     QFVPGHVHLHEIGQQVKAEIEKLGCFAAEFNTIAIDDGIAMGHDGMLYSLPSRDIIADS
                     VEYMVNAHKADAMVCISNCDKITPGMLMASMRLNIPTIFVSGGPMEAGELDNRHLDLID
                     AMIESADTSVSDERIAQVERNACPGCGCCSGMFTANSMNCLNEAIGLALPGNGTIVATH
                     VGRTQLFRDAARQIVENAYKYYRDGDESVLPRSIATRQAFLNAMSLDIAMGGSTNTVLH
                     LLAIAQEAEAGFTMDDIDMLSRKTPCLCKVAPNTHTYHVQDVNRAGGIMGIMNELLKSG
                     LVDGNVKRVDGMTLAEAVDKFAITSPNMTDEAVKKYKSAPAHRFSIRMGSQETYYKELD
                     TDRAAGCIRDIEHAYSKDGGLAVLKGNIALDGCVVKTAGVDESIWKFAGPAKVFDSQDA
                     ACEGILGGKVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGKECALITDGRFSGG
                     TSGLSIGHISPEAAAGGAIGLVKDGDIIEIDIPERTINVKVSDEELAERRKAEEARGTK
                     AFTPPHRQRVVSKALRAYGKMVSSADKGGVRVVED"
     CDS             1925..3625
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 4e-52, bitscore:
                     163.8, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKQKITGSEALLKSLIAEGVDTIFGYPGGQAIPIYDSLYDYRDK
                     LKHVLVRHEQGATHAAQGYARVSGKVGVTLVTSGPGATNTITGIADALMDSTPMVVIAG
                     QVPSPLLGTDAFQEVDVIGITQPITKWAYQIRKPEEIAWAVSRAFYIASTGRPGPVVLD
                     LAKDAQVGFVEYEYKKVDYIRSYQPEPDINQDRIKAAAALINKAEKPFCLVGQGVLLGG
                     AEEELKAFLLKNDIPAGSTVLGLSALPSDFPLNKGMLGMHGNVGPNRKTNECDVLIAIG
                     MRFDDRVTGDLETYAKQAKVIHLDIDNSEIGKNVPVDVKVLGNAKHTIPMITALLEERK
                     RPEWNAEFEPDAQEEYEKVIEKELYPMNGSLKMGEVVRKVSEATGNDAVLVTDVGQNQM
                     MGVRYFKYKRTRSVVTSGGLGTMGFGLPAAMGAKFGAPDRTVCFFTGDGGMQMTIQELG
                     TIMQEKLDVKIIILNNNFLGMVRQWQELFFHERYSNTIMENPDFVAIAKAYGIAGRTVE
                     KREELDEAIAEMLNHNGAFVLVANVETCGMVYPMVPAGGSVTNMILGDK"
     CDS             3638..4189
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg250_3"
                     /sec_met_domain="ALS_ss_C (E-value: 2.2e-18, bitscore:
                     54.1, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTKKLYTVIIFSENTVGLLNQITIIFTRRQLNIETLSVSPSAIQ
                     GIHKFTITTFADEDMIDKVVKQIDKRVDILKAYYNTDEDLVFQEIALYKLSTELFIKMG
                     TVEDLIRKYNARILEMNETCVVLEKSGHYDETQALFRELSETIGVLQFIRSGRIAITKS
                     RVERLSDMLAEMERKLQEKK"
ORIGIN
        1 taataatatt taggataaga aaataggggc ggttcgtgag ccgttcgaaa acaacaaata
       61 atatgatgaa gaatccgtta agaagttcat acagcacaga gggcagacgc atggctgggg
      121 ctcgcgcttt gtggcgtgcc aacggtctga aagaagagca gttcggtaaa ccgattatag
      181 ccattgtgaa ttcatttacc cagtttgtac ccggccatgt ccatctgcac gagatcgggc
      241 agcaggtaaa agcggagatt gaaaagttgg gttgctttgc tgccgaattc aatacgattg
      301 ccattgacga tggtatcgct atgggacacg acggaatgct ctattcgctt ccttcgcgtg
      361 atattattgc ggacagcgtc gagtatatgg tgaatgccca caaagccgat gcaatggttt
      421 gcattagcaa ttgcgacaag atcactccgg ggatgctaat ggcatccatg cggctcaata
      481 ttccgactat ctttgtctcc ggcggtccga tggaagccgg tgagctggat aaccgccatc
      541 tggatttgat cgatgccatg atcgaatcgg ctgacacttc ggtcagcgat gaacggatcg
      601 cgcaggtgga acggaatgcc tgtccgggat gcggttgttg ttccggtatg tttacggcca
      661 actcgatgaa ctgcctgaac gaagcgatcg gcctggctct tccgggcaac ggtacgattg
      721 tcgctacgca tgtgggccgt acacaactgt tccgcgatgc tgcccggcag attgtagaaa
      781 atgcctataa atactatcgt gacggtgatg agtctgttct tccccgcagt atcgccaccc
      841 gtcaggcttt cctgaatgcc atgtcattgg atattgcaat gggtggttcc accaatacgg
      901 ttctccactt gctcgccatc gcgcaggaag ctgaggcggg cttcacgatg gacgatatcg
      961 atatgctctc ccgcaagact ccttgcctct gcaaggttgc tcccaacacg catacctacc
     1021 acgtccagga tgtgaaccgt gccggtggta tcatgggaat catgaacgag ctgctgaaaa
     1081 gcggcttggt agacgggaat gtgaagcgtg tggacggtat gacgcttgcc gaagccgtag
     1141 ataaatttgc tatcacctcg ccgaatatga cggacgaggc tgtcaagaaa tacaagagtg
     1201 ctcccgccca tcgtttcagc attcggatgg gatcgcagga aacctattat aaggaactgg
     1261 ataccgatcg tgccgccgga tgtatccgtg atatcgagca tgcttattcc aaagatggcg
     1321 gcctggctgt cctgaaagga aatatcgcgc tggacggttg tgtggtcaag acggccggtg
     1381 tggatgaaag catctggaag tttgctggtc cggcgaaagt gttcgattcg caggatgcgg
     1441 cttgcgaagg catcctcggt ggaaaagtcg tttcgggcga tgtggtggtg atcacacacg
     1501 aaggaccgaa gggtggtccg ggtatgcagg aaatgcttta tcctacttct tatataaaaa
     1561 gccgccattt aggcaaggag tgcgcgctga tcacagacgg tcgtttcagt ggcggtactt
     1621 ccggtctttc tatcggtcat atttctcccg aagctgcggc aggaggagcg attggactgg
     1681 tgaaagacgg cgatataatc gagattgata tccctgaacg tacgattaat gtgaaggtaa
     1741 gcgatgaaga gttggcagaa cgccgtaaag cggaagaagc ccgcggcaca aaagccttta
     1801 cgccgcctca tcgccagcgt gtcgtctcaa aagccttgag agcttacggt aagatggtca
     1861 gttcggccga taagggtgga gtgagagtag tagaagatta aacacaagac taaaataaag
     1921 agatatggag aaacagaaga taaccggttc ggaagctttg ctgaagtcac tgatagctga
     1981 aggagtagat acgattttcg gttatcccgg cgggcaagcg attcctattt acgatagttt
     2041 gtacgattac agggataaat tgaagcatgt attggtgcgt catgaacaag gggcgacgca
     2101 tgctgcacaa gggtatgccc gtgtatccgg taaagtaggg gtgacgttgg ttacttcagg
     2161 acccggcgca acaaatacca tcaccggcat tgccgatgca ctgatggaca gcactcctat
     2221 ggtggtcata gccggacagg tcccttctcc attgcttgga acagatgctt tccaggaagt
     2281 cgatgtgatc ggcattacgc aaccgatcac caaatgggcg taccagattc gcaagccgga
     2341 agagatagcg tgggctgttt cacgtgcttt ttatatcgct tctaccggac ggccggggcc
     2401 tgttgtactc gatttggcaa aggatgctca ggtcggattt gtggagtatg aatataagaa
     2461 agtagactat atccgcagct atcagccgga accggatatc aaccaggatc ggattaaagc
     2521 ggctgccgcg ttgatcaata aagcggagaa accgttctgc ctggtcgggc agggtgttct
     2581 gttgggaggt gctgaagagg agctgaaagc attcctgctg aagaatgaca ttcctgccgg
     2641 ttcgactgtt ctcggtctat cggcgttgcc ttccgatttt cctttgaata agggtatgtt
     2701 gggtatgcac ggcaatgtcg gtccgaatag gaagaccaat gaatgtgacg tgttgatcgc
     2761 catcggtatg cgtttcgacg atcgtgttac tggcgatttg gagacatacg ccaagcaggc
     2821 gaaagtgatc catttggata tcgacaactc ggagatcggg aagaatgtac cggtcgatgt
     2881 gaaggttctc ggaaatgcca aacataccat tccgatgata actgcgttgt tggaagaacg
     2941 gaaacgtccg gagtggaatg ctgagtttga gcctgatgca caggaagagt atgaaaaggt
     3001 gatcgagaag gagttgtatc cgatgaacgg atctttgaaa atgggtgaag tcgttcgaaa
     3061 agtgtccgag gcgactggaa acgatgctgt tttagttacg gatgtcggac agaaccagat
     3121 gatgggtgtc cgctatttca aatataaacg cactcgtagc gtggttactt caggtggtct
     3181 ggggacgatg ggctttggcc ttcccgctgc tatgggagcc aagttcggtg cacctgaccg
     3241 tacggtctgc ttctttaccg gtgacggtgg gatgcagatg acgattcagg agttgggaac
     3301 cattatgcag gaaaaactgg atgtgaaaat catcatcctt aataataatt tcttaggaat
     3361 ggtacgtcag tggcaggagt tgttcttcca cgaacgttat tcgaatacga ttatggagaa
     3421 cccggatttc gtggcgatcg cgaaagcgta cggtatcgct ggccgcaccg ttgaaaaacg
     3481 ggaggaactg gatgaggcta ttgctgagat gttgaaccat aacggagcgt ttgtgttggt
     3541 cgccaatgtg gaaacgtgtg gtatggtata tccgatggtt cctgctggag gaagcgtaac
     3601 gaatatgata ttaggagata agtaaggagg tgaagctatg acaactaaaa aattatatac
     3661 cgttattatc ttttcagaga atacagtagg tcttctgaac cagattacga ttatctttac
     3721 gcgtcggcag ttgaacatcg agacgctttc cgtttcacct tccgccatac agggtataca
     3781 taaatttacg atcacgacat ttgccgatga agatatgatc gacaaggttg tgaagcagat
     3841 cgacaaacgg gtggatatct tgaaggctta ctataatacg gatgaagatc tggttttcca
     3901 ggagatcgcc ctttataagc tgagcaccga gctgtttatc aagatgggga cggtcgagga
     3961 tctgattcgc aaatataacg cccgtattct ggaaatgaac gaaacgtgtg tcgttctcga
     4021 aaagagtggt cattacgatg agacgcaagc ccttttcagg gagttgagcg aaacgatcgg
     4081 tgtcttgcag ttcatccgtt ccggtcgcat tgcgatcaca aaaagccgtg tcgagcgttt
     4141 gagcgatatg ttggccgaaa tggaacgtaa gttgcaggaa aaaaaataac gtacgggtgg
     4201 tttgcgaact gccccaacag aaaagaatca tggataaaga taaaagcaaa at
//
