LOCUS       c00423_gnlPro..         3198 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PKKOEMMK_423.
ACCESSION   c00423_gnlPro..
VERSION     c00423_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 09:42:45
            Original ID  :: gnl|Prokka|PKKOEMMK_423
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3198
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3198
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2973]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2973
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3198
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3198
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..716)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73.4;
                     E-value: 7.1e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg423_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.1e-36, bitscore:
                     113.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEYVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWVR
                     HVVVPGLTDNDEDLEKLAGYVSRYKVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLTNAKAIFSKHGI"
     CDS             complement(733..2973)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg423_2"
                     /sec_met_domain="Gly_radical (E-value: 3.2e-47, bitscore:
                     146.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.8e-283, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFEKMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDRDLLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
ORIGIN
        1 gttatcccgt gtttactaaa gatcgcttta gcattggtca gacgttctgc cgaaagcggt
       61 tccacacctt tcagcttata atccaggccc tgcgcctcat acttataggc tcccattgtg
      121 tgataaggca aaagctcggc tttctgaacc actttatagc gactgacgta tccggctaat
      181 ttttccagat cctcatcatt atccgtcagg ccgggaacga ccacatggcg gacccatacg
      241 ggaatgcctc gtttctccag ttcatcaaga aaacgcaagg tgttatcaag cttcacagca
      301 gtcagacggg gatgaagccc ggcgtcgatc gtcttgatgt ccaacaaaac caaatcgaca
      361 tattcaagga cttccaatgc tttaccggaa cagatatagc cggaagtgtc caacgccgta
      421 tgaataccgt tctcacggca aaggcggaaa aactctttaa ggaattcagg ttgtaacaaa
      481 ggctcgccac cggtcacggt aacaccacct ttagcgataa aattcttata gcgtagcact
      541 tcggcaagca acgcttcagg ttccagcaga taaggcgttt cacgctttac ctcccaagta
      601 tccggattgt ggcaatacag acaacgaagc ggacatccct gcataaaaac gacaaagcga
      661 attccgggac cgtcaacggt cccgaaactc tctaaagaat gtatacgccc caacatatta
      721 atcaatggac aattataaac tgctgttgat ggtgcgcgag atgacgtcaa gctgctgttc
      781 gcgtgtcagc ttcacgaagt tgacggcata gccagaaacg cggatcgtaa gctgcgggta
      841 tttctccgga tgctccattg catctaacaa caagtcccta tcgaacacgt tcacattcag
      901 atgctggcca ccgtcaggcg taaaatagcc atccaacagg ctcaccaaat tatttatctg
      961 aatatcgcgc tccttaccca acgtagccgg tgaaacggca aatgtatagg aaataccgtc
     1021 atgcgcatgc tgaaaaggca gtttggctac agaagccaaa gcggcgacag cccctttcgt
     1081 atcgcgtccg ttcatcgggt tagctcccgg agcgaacggc gtaccgcccg gacggccgtc
     1141 gggagtcgtt ccggttttct ttccgtaaac cacgtttgat gtgatcgtca ggatagattg
     1201 tgtcggagtt gccttgcggt atgtctgatg ctggcgcaga tattccatga acttctcggt
     1261 aatgtcaaca gcaatactgt ccgtgcggtc gtcattgttt ccgaaaggta catattcgcc
     1321 ttcgcgttcg aagtcgacag ccaaaccacg ttcgtcacgg atcactttca ctttcgtatc
     1381 acggatggca gccagcgagt cggcaacgat agaaaggccg gcgataccgg ttgcgcggat
     1441 acgttccaca tctccgtcat gaagccccat ctcgaatgct tcatacgcat atttgtcgtg
     1501 catgtagtga atgatcttca gtgcattgac atagactttt gccaaccagc gcatcatctg
     1561 ctcgtatttc tccatcactt cgttgtaatc cagatactcg ctggtgatcg gttcgaaacg
     1621 gggagccacc tgcacacccg atttctcgtc acgtccgcca ttgatcgcat acaacaagca
     1681 cttggccagg ttggcacgag ctccgaaata ctgcatctgc ttaccgatct tcatcggaga
     1741 cacacagcag gcgataccgt aatcgtcgcc atagtccgga cgcatcagat cgtcgttctc
     1801 gtactggatg gcagaggtat cgatcgatac ttttgcgcag aagtttttcc agttatccgg
     1861 agcattgttg aaccaaagca ccgtcaggtt gggttcgggt gccgggccga ggttatacaa
     1921 ggtatgcaga taacggtaag atgtctttgt caccagcgaa cggccgtcaa caccttgtcc
     1981 gcccagcgat tcagtcaccc aaacagggtc gccggagaac aggtcgttgt attccggagt
     2041 acgcaggaaa cggacaatac gcagtttgat aataaactgg tcgatcaatt cctgtgcctc
     2101 ttcttccgtc agcttgcctt cgcaaatatc tttttcgata tagatatcga ggaaagtaga
     2161 agtacgtccg agagacatgg ctgctccatc ctgatccttt acggctgcca ggtaagccag
     2221 atacaagaac tgcacggctt cgcgggcatc ctttgccgga cgtgtcacat cgaaaccata
     2281 accggcacac attttctcaa atgctttcag cgctttgatc tgctccgtta tctcctcacg
     2341 actctgaatg atttcttctg tcagttcctg tatatcgaga cgtttctgga aacgtttctt
     2401 ttcttcgatc aggatctggg ttccgtacag aggtacacga cggtaatcac cgatgatacg
     2461 tccgcgtccg taggcatcag gcaacccggt aatgatggcg gaacgacgtg ccgcaatcat
     2521 ctcgtctgta taggctgaaa aaacgccttc gttgtgcgtc tttctgtatt tggtgaagat
     2581 ttcttttgtc atcgggtcca gatgataacc ataggcttcc agactgtttt cgaccatgcg
     2641 gataccgcct ttcgggaaga taccgcgttt caacggggcg tctgtctgca agccgacgat
     2701 tacttcgttt tccttatcga tgtaaccggg tccgtatgtg tcaatcccct ggggaagctt
     2761 cgtttcggca tcatatactc ctttctccct ttcaacctta aacatctcgg ttagtttgtt
     2821 ccacactttc ttcgttttct ccgaagaggg tacgaggaac gtatcgtctc cgttataagg
     2881 tttgtaattg ttctggataa agtctcttac attgatctcc tttttccagg tttctccttt
     2941 gaactgcgcc cagttctcgt tttcaaactt cataagctta tcttgtctat ttcggtttag
     3001 gcacgcaaag atacgtcttt tccccctatc tgaaaaggat tgtaaaggac gagggatgtt
     3061 aaaccggaga aaatacctac aaatagggat aaggggaata aaaacgaagc cccggcagta
     3121 cgaataccgc cggggcctgt tttttgtctg ccgtccggtc ttcacagagg gaaaagcagt
     3181 agaactttta atttcatt
//
