LOCUS       c00298_gnlPro..         5335 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PPNKKEPM_298.
ACCESSION   c00298_gnlPro..
VERSION     c00298_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 11:23:05
            Original ID  :: gnl|Prokka|PPNKKEPM_298
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5335
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..609)
                     /locus_tag="ctg298_1"
                     /transl_table=11
                     /translation="MLETALKDQLRTIFAPLDSRYTFLIRLNPRHENRNELIELLEDVA
                     SCSEKINYRLEEGEGLEFELLKNEQKTRIKFRGIPNGHEFTSLLLAILNSDGKGKNIPD
                     GNLQKRIAALKGPIRLTTYVSLTCTNCPDVVQALNLMAILNDNIEHEMVDGAINQAEVE
                     ALHIQGVPSVYADGKLIHIGRGEFGELLEKLEATYGSENT"
     protocluster    1..5335
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1855:4835]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1856..4835
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5335
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5335
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(706..1272)
                     /locus_tag="ctg298_2"
                     /transl_table=11
                     /translation="MEPIINSQVPEFKVQAFQNGSFKTVSSEDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYGKFQEMGVEVYSVSTDSHFVHKAWHDASESIRKIKYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFVVNPEGQIKVVEIHDNNIGRDANELLRKVEAAQFVATHT
                     GEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             1548..1853
                     /locus_tag="ctg298_3"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCKGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
     CDS             complement(1856..2578)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.4e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg298_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.2e-36, bitscore:
                     114.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKR
                     ETPYLLEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGVTV"
     CDS             complement(2595..4835)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg298_5"
                     /sec_met_domain="Gly_radical (E-value: 4.8e-46, bitscore:
                     144.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.6e-282, bitscore:
                     926.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFERMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLVDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             complement(5263..5334)
                     /locus_tag="ctg298_6"
                     /transl_table=11
                     /translation="IAAPAGVVVVAVEGEAAVKAIVK"
ORIGIN
        1 ggtattctcc gaaccgtagg tcgcctccaa tttttcaagg agttctccga attctccccg
       61 tccgatgtgt atgagcttac cgtcggcata taccgaaggc actccctgta tatgcagagc
      121 ctctacttcg gcttgattta tcgctccgtc taccatttcg tgttctatgt tgtcgttcag
      181 gatagccatc agattcaagg cctgtacaac atccggacaa ttcgtacatg ttaaggatac
      241 ataggtagtt aaccggatcg gtcccttcaa ggctgctata cgtttctgta agttcccgtc
      301 gggtatgttt ttccctttgc cgtcgctgtt gaggatggcc aatagtaaag aagtgaattc
      361 gtgaccgttc ggtatgcccc ggaatttgat cctcgttttt tgttcatttt tcagcaattc
      421 aaattccagc ccctctcctt cttccagccg gtaattgatt ttttctgaac aagaggcgac
      481 atcctctaat agttcgatca attcattccg gttttcgtgt cggggattaa gccggattaa
      541 aaaagtgtat cgggaatcca gcggtgcaaa aatggtgcgt aactggtctt ttaatgctgt
      601 ttctaacata gcgtatattt tttcagaaga tgataaaggg tttcaatggc aggcagactt
      661 ttcagcaacc ggtcggacca ttgaaaccct gaaattagtc cgggattaaa ttttaccgac
      721 taagtcgata ctgggcttta aggtcgcttc accttttttc catttagccg ggcaaacttc
      781 accggtatga gtggctacga attgagccgc ttcaactttt cttaaaagtt cgttcgcgtc
      841 cctcccgatg ttattgtcat gaatttctac aactttgatc tggccttccg gattcacgac
      901 aaaagttccc cggtaagcca ttccttcttc ctcgatcatg actccgaacg cacggcttaa
      961 agctcctgtc gggtctgcca acatcgggta tttaatcttg cggatgcttt cggaagcatc
     1021 atgccatgct ttatgtacaa agtgagagtc tgtacttacg gaatatactt cgacacccat
     1081 ttcctgaaat ttgccgtact tgtcagccat atcgaccaat tcggtcggac atacgaacgt
     1141 aaagtcggca ggatagaaga aaaagatggc ccatttgccc ttgatatctt cgctacttac
     1201 tgtcttgaaa ctaccattct gaaaagcttg aactttgaat tcgggtactt gtgaattgat
     1261 aattggttcc atgattttta ctatttaatt tgttactact atttttgttt accctacaaa
     1321 ggtaaatcgt aagatactga ttgaaagaat ttttcaaatt gataattttt atctgccgat
     1381 agacggattt ctatctgcaa gcatacttat ctactatgac aataggaagc aaagtaatgt
     1441 tttccggaac aataaaaata tacctaattg taggtaaaat acctttcgat cataccatgt
     1501 gattctttaa ttgttaactt tggatgttaa cttaaaaata ttcgaagatg agaagagcta
     1561 gtttgtataa tacattttca gcaagtctat tgctcgtttg caacgcatgt ctttcttcat
     1621 gtgtctcaca aatacccctt acaaaggaaa aaccggtaaa caattccact tatcaagtcg
     1681 aatatctgtt cgaacacgaa gggtgtaagg tctatcgttt catggatatg ggacattaca
     1741 tctatttcac caactgcaag ggagatgtta ccagtattga aagcgacagc gtccgcacag
     1801 tcaaccggat cagccgtaaa ccgatcatca tcaaagagca gacactcgat taggtttata
     1861 cggttacccc gtgtttcata aagatcgctt tcgcattggc cagacgttct gctgaaagcg
     1921 gttccacacc tttcagctta tagtccaggc cttgcgcctc atatttgtaa gctcccattg
     1981 tatgataagg cagaagttcg gctttctgga ccacgttata tgaactgata tattcggcca
     2041 gcttgctcaa ggcttcatca tcgtccgtca ggccgggcac gatcacgtgg cgaatccata
     2101 cagggatgcc gcgtttctcc agttcatcaa gaaagcgtaa agtattatcg agttttacgg
     2161 ccgtcaaacg gggatgaaga ccggcgtcga tcgtcttgat atctaacaga accagatcga
     2221 cctgttcgag cacctccaat gctttaccgg aacagatata gccggaagtg tccaacgccg
     2281 tatgaatacc gttctcacgg caaaggcgga aaaactcttt aaggaattca ggttgtaaca
     2341 aaggctcgcc accggtcacg gtaacaccac ctttagcgat aaaattctta tagcgcagca
     2401 cttcggcaag caacgcttca ggttccagca gataaggcgt ttcacgcttt acctcccagg
     2461 tatccggatt gtggcaatac agacaacgaa gcggacatcc ctgcatgaaa acaacaaagc
     2521 gaattccggg accgtcaacg gtcccgaaac tctctaaaga atgtatacgc cccaacatat
     2581 taatcaatgg acaattataa actgctgttg atggtgcgcg agatgacgtc aagctgctgt
     2641 tcgcgtgtca gcttcacgaa gttgacggca tagccagaaa cacggatcgt aagctgcggg
     2701 tatttctccg ggtgctccat cgcatcgacc aacaggtcct tatcgaacac gttcacattc
     2761 agatgctggc cgccgtcagg cgtgaaatag ccatccaaca ggctcaccag attatttatc
     2821 tgaatatcgc gctccttacc caatgtagcc ggtgaaacgg caaatgtata ggagataccg
     2881 tcatgcgcat gctggaaagg cagtttggca acagaagcca aagcggcgac agcccctttc
     2941 gtgtcgcgtc cgttcatcgg gttggctccc ggagcgaacg gcgtaccacc cggacggccg
     3001 tcgggagtcg ttccggtttt ctttccgtaa accacgtttg atgtgatcgt caggatagat
     3061 tgtgtcggag ttgccttgcg gtatgtctga tgctggcgca gatattccat gaacttctcg
     3121 gtaatgtcga cagcaatact gtccgtgcgg tcgtcattgt ttccgaaagg tacatattcg
     3181 ccttcgcgtt cgaagtcgac agccaaacca cgttcgtcac ggatcacttt cactttcgtg
     3241 tcacggatgg cagccagcga gtcggcaacg atagaaaggc cggcgatacc ggttgcgcgg
     3301 atacgttcca catctccgtc atgaagcccc atctcgaatg cttcatacgc atatttgtcg
     3361 tgcatgtagt gaatgatctt cagagcattg acataaactt ttgccaacca gcgcatcatc
     3421 tgctcgtatt tctccatcac ttcgttgtaa tccagatact cgctggtgat cggttcgaaa
     3481 cggggagcca cctgcacacc cgatttctcg tcacgtccgc cattgatcgc atacaacaag
     3541 cactttgcca ggttggcacg agctccgaaa tactgcatct gcttaccgat cttcatcgga
     3601 gacacacagc aggcgatacc gtaatcgtcg ccatagtccg gacgcatcag atcgtcgttc
     3661 tcgtactgga tggcagaggt atcgatcgat acttttgcgc agaagttttt ccagttatcc
     3721 ggagcattgt tgaaccaaag caccgtcagg ttgggttcgg gtgccgggcc gaggttatac
     3781 aaggtatgca gataacggta agatgtcttt gtcaccagcg aacggccgtc aacaccttgt
     3841 ccgcccagcg attcagtcac ccaaacaggg tcgccggaga acaggtcgtt gtattccgga
     3901 gtacgcagga aacggacaat acgcagtttg ataataaact ggtcgatcaa ttcctgtgcc
     3961 tcttcttccg tcagcttgcc ttcgcaaata tctttttcga tatagatatc gaggaaagta
     4021 gaagtacgtc cgagagacat ggctgctcca tcctgatcct ttacggctgc caggtaagcc
     4081 agatacaaga actgcacggc ttcgcgggca tcctttgccg gacgtgtcac gtcgaaacca
     4141 taaccggcac acattctctc aaatgctttc agcgctttga tctgctccgt tatctcctca
     4201 cgactctgaa tgatttcttc tgtcagttcc tgtatatcga gacgtttctg gaaacgtttc
     4261 ttttcttcga tcaggatctg ggttccgtac agaggtacac gacggtaatc accgatgata
     4321 cgtccgcgtc cgtaggcatc aggcaacccg gtaatgatgg cggaacgacg tgccgcaatc
     4381 atctcgtctg tataggctga aaaaacgcct tcgttgtgcg tctttctgta tttggtgaag
     4441 atttcttttg tcatcgggtc cagatgatag ccataggctt ccagactgtt ttcgaccatg
     4501 cggataccgc ctttcgggaa gataccgcgc ttcaacgggg cgtctgtctg caagccgacg
     4561 attacttcgt tttccttatc gatgtaaccg ggtccgtatg tgtcaatccc ctggggaagc
     4621 ttcgtttcgg catcatatac tcctttctcc ctttcaacct taaacatctc ggttagtttg
     4681 ttccacactt tcttcgtttt ctccgaagag ggtacgagga acgtatcgtc tccgttataa
     4741 ggtttgtaat tgttctggat aaagtctctt acattgatct cctttttcca ggtttctcct
     4801 ttgaactgcg cccagttctc gttttcaaac ttcataagct tatcttgtct atttcggttt
     4861 aggcacgcaa agatacgtct tttcccccta tctgaaaagg attgtaaagg acgagggatg
     4921 ttaaaccgga gaaaatacct acaaataggg ataagggaaa taaaaacgaa gccccggcag
     4981 tacgaatacc gccggggcct gttttttgtc tgccgtccgg tcttcacaga gggaaaagca
     5041 gtagaacttt taatttcatt actttagtat tattaatatt aaatccaacc gctcgttcgc
     5101 ctgtcgcagg gtaattgaaa ccgtagatca agaaaacctc ttacatacgg tttcacgcag
     5161 gttgtctaga attatttgat tataaaatgt cattgtgcct gtgtaagggt ggttcgctaa
     5221 ccacccttac gatagacagt attgtttttt tagtatcaat cgttacttaa cgatagcttt
     5281 tacagcagct tcgccttcaa cagctacaac tactacacct gccggagcag cgatt
//
