LOCUS       c00326_gnlPro..         4359 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LPLLDEMI_326.
ACCESSION   c00326_gnlPro..
VERSION     c00326_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 08:29:08
            Original ID  :: gnl|Prokka|LPLLDEMI_326
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4359
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4359
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[542:4357]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      543..4357
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..4359
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4359
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             complement(3..542)
                     /locus_tag="ctg326_1"
                     /transl_table=11
                     /translation="MSDNKKIGSYKFIAEPFHVDFNGRLTMGVLGNHLLNCAGFHASER
                     GFGIATLNEDNYTWVLSRLAIDLEEMPYQYEEFTVQTWVENVYRLFTDRNFAIIDKDGK
                     KIGYARSVWAMINLNTRKPADLLTLHGGSIVDYVCDEPCPIEKSSRIKVATDQPCAKLT
                     AKYSDIDINGHVNSIRY"
     CDS             complement(543..1103)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg326_2"
                     /sec_met_domain="ALS_ss_C (E-value: 1.3e-19, bitscore:
                     58.4, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKTLYTLIVHSENFAGLLNQVTAVFTRRQINIESLNVSASSIKG
                     VHKYTITAWTDKDTIEKVVKQIEKKIDVLQAHYFTEDEIYFHEIALYKVSMPEFQSQPE
                     ASKVIRRYNARIVEVNPVFAIVEKNGISEEITSLYEELSALNCVLQFVRSGRVAITTSC
                     FERVNEFLADRETKYNLSKKEEK"
     CDS             complement(1117..2814)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg326_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 3.2e-48, bitscore:
                     151.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKDIISGGEALMRSLEYHGVKTIFGYPGGSIMPVFDALYDHRET
                     LNHILVRHEQGAAHAAQGFARASGEVGVCLVTSGPGATNTITGIADAMIDSTPIVVIAG
                     QVGTAFLGTDAFQEVDLVGITQPITKWSYQIRRAEDVPWAVARAFYIAKSGRPGPVVLD
                     FAKNAQVEKAEYMPAKLDFIRSYVPVPETDPEAVKAAAELINGAERPLVLVGQGVELGN
                     AQQELRAFIEKADMPAGCTLLGLSALPTEHPLNKGMLGMHGNLGPNMNTNKCDVLIAVG
                     MRFDDRVTGNLATYAKQAKVIHFDIDPAEINKNVHADVAVLGNCKETLSAVTALLQPNE
                     HKEWLDSFLPYEQVEEEKVIRPELHPMGDTLSMGEVVRAVSDATNHEAILVTDVGQNQM
                     MSARYFKYSKDRSMITSGGLGTMGFGLPAAIGATFGRPDRTVCVFMGDGGLQMNIQELG
                     TIMEQKAPVKIIVLNNNFLGNVRQWQAMFFNRKYSFTPMLNPDYIKIASAYDIPAKRVF
                     TREELADAIDEMIATDGPFLLEACVIEEGNVLPMTPPGGSVNQMLLEC"
     CDS             complement(2870..4357)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg326_4"
                     /sec_met_domain="ILVD_EDD (E-value: 1.7e-183, bitscore:
                     598.9, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EYMVNAHKADAMVCISNCDKITPGMLMAAMRLNIPTVFVSGGPME
                     AGELDGQHLDLIDAMIKSADESVSDEEVSKIENRACPTCGCCSGMFTANSMNCLNEAIG
                     LALPGNGTIVATHANRTQLFKDAAKLIVENTYKYYRDGDESVLPRSIATREAFLNAMTL
                     DIAMGGSTNTVLHLLAIAHEAEADFKMDDIDMLSRKSPCLCKVAPNTQKYHIQDVNRAG
                     GIMGIMGQLAKAGLIDTSVVRIDGMTLGEAIDKYDITSPNVCEEAIKKYKSAAAGKFNL
                     VLGSQDVYYKELDTDRAEGCIRDIEHAYSKDGGLAVLKGNIAQDGCVVKTAGVDESIWK
                     FTGPAKVFDSQEAACNGILGGKVVSGDVVVITHEGPKGGPGMQEMLYPTSYIKSRHLGK
                     ECALITDGRFSGGTSGLSIGHISPEAAAGGNIGKIKDGDIIEINIPERTINVRLTDEEL
                     AARPMTPVTRERYVPKSLKAYASMVSSADKGAVRLID"
ORIGIN
        1 atataacgaa tgctgttcac atgaccattg atgtcgatat cgctgtattt agctgtcaac
       61 ttagcacaag gttggtcagt agccactttg atacgcgaag acttttcgat cggacaaggt
      121 tcgtcacata catagtccac aatactaccg ccatgcaacg tcagcaaatc agccggcttg
      181 cgggtattta aattaatcat ggcccatacc gaacgggcat atccgatctt cttgccgtcc
      241 ttgtctataa tggcaaaatt acgatcagta aaaagacgat atacattctc cacccatgtc
      301 tgtacagtaa actcctcata ttgataaggc atctcttcca gatcgatggc cagacgagac
      361 aatacccatg tataattgtc ctcattcaat gtagcgattc caaagccgcg ttcactggca
      421 tgaaaaccgg cacagttcaa cagatggttg ccaagcaccc ccattgtcaa acgtccgttg
      481 aagtctacat gaaaaggctc cgcaataaac ttataactcc ctatcttctt attgtcactc
      541 attcactttt cctctttctt gcttaagtta tatttggtct cacgatctgc cagaaactcg
      601 ttcacacgtt caaagcaact ggtagtgatt gccacacgtc ccgaacgcac aaattgcaac
      661 acacaattca atgcactcaa ctcttcgtag agagaagtaa tttcttcact gattccattt
      721 ttctcgacga tggcaaatac cggattcact tccacaatcc gtgcattata gcgacgaatc
      781 accttcgaag cttccggctg tgactgaaat tcgggcatag acaccttata aagagctatt
      841 tcatgaaaat agatttcatc ttcagtgaaa taatgtgcct gaagcacatc gatcttcttc
      901 tcaatctgtt tgacaacctt ctctatcgta tctttatcgg tccaagccgt aatagtatat
      961 ttatgcaccc ccttgataga tgaagccgaa acattcaggc tttctatatt aatctgccga
     1021 cgggtaaata ctgccgtaac ctgattcaac aatccggcaa agttctctga atgaacaatt
     1081 aatgtatata atgtcttatc cataattcct aacttattag cattccaaca acatctggtt
     1141 aactgaacct cccggagggg tcatcggcaa cacatttccc tcttcaatca cacaagcttc
     1201 caacaggaac ggaccgtctg tcgcaatcat ctcgtcaatc gcatcagcca gttcttcacg
     1261 ggtaaagact cttttggccg gtatatcata agcagaagca atcttaatat aatcgggatt
     1321 cagcatgggt gtgaacgagt atttacggtt gaagaacatt gcctgccatt ggcgtacatt
     1381 gcccaggaaa ttattgttta atacgataat tttaaccggt gccttctgtt ccataatggt
     1441 tcccaactcc tgaatgttca tttgcagacc accgtcaccc ataaatacac aaaccgtacg
     1501 gtccggtcgg ccaaaggttg caccgatagc agccggcaac ccgaatccca tggtaccaag
     1561 tcctccggaa gtaatcatac tccggtcttt tgaatattta aagtatcggg cagacatcat
     1621 ctggttttga ccgacatcgg tcaccaatat cgcttcatga ttggtcgcat cgcttaccgc
     1681 acgcaccact tcccccatcg aaagcgtatc tcccatcgga tgcaattccg gacggattac
     1741 cttttcttct tccacctgtt cataaggcag gaaactgtcc agccattctt tatgctcgtt
     1801 gggctgcagc aatgccgtaa cagcagagag cgtctcttta cagttaccca gaaccgctac
     1861 atccgcatgt acattcttat taatttcagc cggatcgata tcaaaatgaa tcactttcgc
     1921 ctgcttggcg tacgtagcca ggtttccggt cacacggtcg tcaaaacgca tgccgacagc
     1981 aatcaggaca tcacatttat tggtgttcat attcggtccg agattaccgt gcattcccag
     2041 catacctttg ttcaaaggat gctcggtcgg caaagccgac agtcccaaca acgtacatcc
     2101 ggcgggcata tctgcctttt cgatgaaagc acgcaattcc tgttgtgcgt tgcccagttc
     2161 cacaccttgc cccaccaata ccaacgggcg ctctgcaccg ttaattaatt cggcagccgc
     2221 tttcacggcc tccgggtcgg tctcgggtac aggcacgtaa ctgcgtatga aatcgagttt
     2281 cgcaggcata tattcggctt tctccacctg ggcatttttt gcaaagtcga gcactaccgg
     2341 tcccgggcgt ccgcttttcg ctatataaaa ggcgcgggcc accgcccatg gcacatcttc
     2401 ggcacgacgg atctgataac tccattttgt aattggctgc gtgatgccta ccaagtcgac
     2461 ctcctgaaaa gcatctgtcc ccaaaaaagc tgttcccacc tgtccggcaa tgactacaat
     2521 cggtgtactg tcgatcattg catccgcaat accggttatg gtattggtag caccggggcc
     2581 gctcgtcacc aggcaaactc cgacctcacc cgaagcacgg gcaaatccct gagccgcatg
     2641 tgccgcccct tgttcgtggc gaaccaaaat atgattcaaa gtctctctat gatcgtataa
     2701 ggcatcgaat acgggcatga tagatcctcc gggataacca aagatcgtct ttacaccatg
     2761 atattccaaa gaacgcatta atgcttcacc gcctgaaatt atatctttac tcatgtacta
     2821 aaatttacga ttttacgatt tacaatttac gattgaaaca actctgtggt taatcaatca
     2881 gtctcaccgc tcctttatcg gcagagctta ccatgctggc atacgcttta agacttttag
     2941 gcacatagcg ttcacgagtg accggagtca tcggacgtgc agccagttct tcatcggtca
     3001 gtcgcacgtt gatagtccgt tcggggatat tgatttcgat gatatcacca tccttaatct
     3061 ttccgatatt acctccggct gctgcttcgg gagagatatg cccgatgctg agtcccgatg
     3121 tacctccgct gaaacggccg tcggttatca gcgcacactc cttgcccaag tggcgcgatt
     3181 tgatataaga ggtaggataa agcatttcct gcataccggg acctcccttc ggaccctcgt
     3241 gcgtgatgac gactacatct ccgctgacaa cttttcctcc gagtatccca ttgcaggctg
     3301 cttcctgtga atcgaacacc tttgccggac cggtaaactt ccagatgctc tcgtccactc
     3361 cggccgtttt caccacacaa ccatcctggg caatatttcc tttcagtacg gccagtcctc
     3421 cgtctttgct atatgcgtgc tctatgtcgc gaatacaccc ttcggcacgg tccgtatcca
     3481 gctctttata atagacgtcc tgagagccga gcaccagatt gaactttccg gcagccgcac
     3541 tcttatactt tttaatagcc tcttcacata cgttcgggct agttatatca tatttatcta
     3601 tcgcctcacc caatgtcatc ccgtcgatac gcactacaga cgtatcgatc aatccggctt
     3661 tggcaagttg tcccataatc cccatgatac ctccggcacg gtttacatcc tgaatatgat
     3721 acttctgtgt attcggcgcc accttacaca agcatgggct cttgcgtgaa agcatatcga
     3781 tatcgtccat tttaaagtct gcctccgctt catgggctat cgccagcagg tgaagtaccg
     3841 tattggtaga tcctcccatt gcgatgtcca gcgtcatggc attcaggaat gcctcacggg
     3901 tggcaatgct ccggggaagt acgctttcat caccgtcccg ataatattta taggtatttt
     3961 ctacaatcag tttcgcagca tctttgaaca attgggtacg gttggcatgg gtcgccacaa
     4021 tcgttccgtt tccggggaga gccagaccga tcgcttcgtt caggcagttc atcgaattgg
     4081 cggtaaacat tcccgaacaa catccgcaag tgggacaggc ccgattctcg atcttggaga
     4141 cttcttcatc actcacactt tcatcggccg acttaatcat cgcatcaatc aggtccaaat
     4201 gctgtccatc cagttcaccg gcttccatcg gacctccgga tacaaataca gtaggaatat
     4261 tcagtctcat agccgccatc aacatccccg gagtgatttt gtcacaattg ctgatacata
     4321 ccatggcatc cgctttatgg gcattcacca tatattcca
//
