LOCUS       c00375_gnlPro..         5249 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JLOKKHCD_375.
ACCESSION   c00375_gnlPro..
VERSION     c00375_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 10:09:25
            Original ID  :: gnl|Prokka|JLOKKHCD_375
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5249
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5249
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1462:5249]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1463..5249
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..5249
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5249
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..128)
                     /locus_tag="ctg375_1"
                     /transl_table=11
                     /translation="MLEEILEFNKKFVENRGYEKYITNKYPDKKIAILSCMDTRLT"
     CDS             246..1229
                     /locus_tag="ctg375_2"
                     /transl_table=11
                     /translation="MPLKLTTYYQGSEIPDFPGTNTFHSKELFQIYEETPGYTPLLIMA
                     SEDGKPVARLLAAIRKSIRMFPPAFIKRCEIYGTGEYLTAEADKERIFGEMLEHLTTEA
                     LRNSFLIEFRNLENAMFGYKYFRSNRYFPVNWLRVRNSLHGIENTEQRFSPSRIRQIKK
                     GLKNGAKVDEARTVEEIREFSNMLRHLYSSRIRKHFPNIIFFQHMDTRLIHSRQAKIFI
                     VRYKDKIIGGSACIYSGNDAYLWFSGGMRKTYALQYPGILAVWKALRDAHQNGFRHMEF
                     MDVGLPFKKHGYRDFVLRFGGKQSSTRRWFRFRWQWLNDLLIKIYV"
     CDS             1463..1867
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg375_3"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.5e-24, bitscore:
                     75.4, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKISHIEHLGIAVKSIEEALPYYENVLGLKCYNIETVEDQKVRTA
                     FLKVGDTKIELLEPTCPESTIAKFIENKGAGVHHVAFAIEDGVANALAEAESKEIRLID
                     KAPRKGAEGLNIAFLHPKSTLGVLTELCEH"
     CDS             1993..3546
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg375_4"
                     /sec_met_domain="CT_Btheta (E-value: 1.4e-291, bitscore:
                     955.9, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIELRAQARLGGGEKAIEKQHAKGKYTARERIAQL
                     LDEGSFEELDMFVQHRCTNFGQEKKHFLGDGVVTGYGTIEGRLVYVFAQDFTVFGGSLS
                     ETMAQKICKVMDMAMKMGAPVIGINDSGGARIQEGINALSGYAEIFQRNIMASGVIPQI
                     SGIFGPCAGGAVYSPALTDFTLMTEGTSYMFLTGPAVVKTVTGEDVSQEDLGGASVHAS
                     KSGVTHFTAETGEEGLAIIRKLLSFIPQNNLEEAPLVNCTDPIDRMDDLLNEIIPDSPN
                     KPYDMYEVIGAIIDNGEFLEVQKDYAKNLIIGFARMNGQSVGVVANQPKYLAGVLDSNA
                     SRKGARFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKVTV
                     TLRKSYGGSHIVMSCKQLRGDMNYAWPTAEIAVMGGAGAVAVLYAKEAKDQENPAQFLA
                     DKEAEYTKLFANPYNAAKYGYIDDVIEPRNTRFRVIRALQQLQTKKLTNPAKKHGNIPL
                     "
     CDS             3567..4481
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg375_5"
                     /sec_met_domain="OAD_gamma (E-value: 8.8e-17, bitscore:
                     50.6, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKRIGIFIALLAMVCMGLRAQSATSLRINEVLVVNDQNYQDDY
                     GLHNAWIEIFNTSFASVNLEGCFLTNDKNNPTKYPIPKGDVLTLIKPRQHALFWADGMP
                     NRGTFHVNFTLDPNKENYIALYDSNGKTLIDEVTIPAGQLADRSYAREKDGSANWVVKG
                     EGEHSYVTPSTNNMTIDKNPKIENFKKHDSIGIGMAIIAMSVVFIGLVLLYLSFKAVGN
                     VAVRLGKKNAMKATGITDKTEAKEKNLGSHTGEETAAIAMALHEYLNDAHDVEDMILTI
                     NKVKRTYSPWSSKIYTLRQTPKR"
     CDS             4507..4938
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 69.7; E-value:
                     4.8e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg375_6"
                     /sec_met_domain="Biotin_lipoyl (E-value: 5.5e-23, bitscore:
                     69.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKQYKYKINGNLYNVTVNDVEDNIANVEVNGTSYKVELDKPVKAA
                     PKTVTRPAAAPKTETGAPVVTKQPTASKKDGVKSPLPGVILDIKVKEGDTVKRGQTIII
                     LEAMKMENNINANKDGKVAEIKVNKGDSVLEGTDLVIIE"
     CDS             4941..5249
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg375_7"
                     /sec_met_domain="OAD_beta (E-value: 3e-22, bitscore: 67.9,
                     seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGDFVSFLGNNLVDFWGYTGFANATPGHLFMLLIGLFFIYLAVAK
                     EFEPMLLIPIGFGILIGNIPFNMEAGLKVGIYEEGSVLNILYQGVTSGWYPPLIFLGI"
ORIGIN
        1 tctgtcaggc gggtatccat acaggaaagg atggcgattt tcttatcagg atacttgtta
       61 gtgatatact tttcgtatcc cctgttctca acgaactttt tgttgaattc aagaatttct
      121 tctaacataa tcaaaaacgg attttattgt agtatctcca caaagatagt cacaaaacga
      181 cagctcaaca aaataagccc tgcaaatgtt gtaaagagaa ataaaaaatg taaatttgtc
      241 ccgatatgcc attaaaactg actacatatt accaaggaag tgagattccg gattttcccg
      301 gaaccaatac gtttcattcg aaagagctat ttcagatcta cgaagaaact ccgggctaca
      361 ccccgcttct gattatggct tcggaagacg gaaaacctgt tgcgcgactg cttgctgcca
      421 tccgcaaaag catccgcatg tttcctcccg ctttcatcaa acgctgcgaa atctatggca
      481 cgggcgaata tctgacagcg gaagccgaca aggaaaggat attcggcgaa atgctggagc
      541 acctcaccac ggaggctttg cggaactcgt ttctgataga attccgcaat ctggagaacg
      601 ccatgttcgg ctacaaatat ttccggtcca acagatactt ccctgtcaac tggctgcggg
      661 tacgaaactc gcttcacggc atcgaaaaca ccgaacagcg tttcagcccc tcccgtatcc
      721 gccagatcaa aaaaggcctg aaaaatggag caaaagtaga cgaggcacgt acggtagaag
      781 agatacgcga attttcgaat atgctgaggc atctctattc gtcccgcatc cgtaaacatt
      841 ttcccaacat catctttttc cagcacatgg acacccggct gattcacagc cggcaggcaa
      901 agattttcat cgtccgctac aaagacaaaa ttatcggagg ctctgcctgt atctattcgg
      961 gaaacgatgc ctatctctgg ttttcgggag gtatgcggaa gacctatgcc ttgcaatacc
     1021 cgggtatact ggctgtatgg aaagccctcc gggatgccca tcaaaacgga ttccgccaca
     1081 tggagttcat ggacgtcgga ctcccattta agaagcacgg ttatcgtgac ttcgtgctcc
     1141 gcttcggtgg caagcaaagc agcacccggc gatggttccg tttccgctgg caatggctga
     1201 acgacctgct gatcaaaata tacgtctgaa catccgcctg tctcagggat cagcgccaac
     1261 cctctccgcc ttttaaacca acacccctta ggttttaaac caacgatgcc tgccttttaa
     1321 acccatgcac cttaggtttt aaacctattt tcaccataat taattgcaga aaaatttctt
     1381 tattaaacaa gaattgattt tctttgtgct gttttatacc attccgaaaa gacgattaat
     1441 accaaaattt aaaaatatag ttatgaagat ttcacacatt gaacatttgg gcattgccgt
     1501 aaaaagtatc gaagaagccc ttccgtatta tgaaaacgtt ttaggtttga aatgctacaa
     1561 catcgaaact gtagaagatc aaaaagtaag aacagccttc ctgaaagtag gtgataccaa
     1621 aatcgaatta ctggaaccca catgtccgga aagtaccatc gcgaaattca tcgaaaacaa
     1681 aggtgcaggt gttcatcacg tggcattcgc cattgaagac ggcgtagcca atgccctagc
     1741 cgaagccgaa agcaaagaga tccgcctgat cgacaaagct ccgcgcaaag gtgccgaagg
     1801 cctgaacatt gctttccttc acccgaaatc aactctgggc gtactgacag aactctgcga
     1861 acactaaaaa gatgtacgat tcggacgatc cgcgccacgg acaaacggaa tttttctttg
     1921 cattaaattc cccatccggg ttgtcaatcg tccggttgta aatattataa ccctttaaat
     1981 taagaaaaac tcatgagtaa ccagcttgaa aaagtaaaag agcttattga acttcgtgct
     2041 caggctcgtt taggtggtgg cgaaaaggct attgaaaaac aacacgccaa aggcaaatat
     2101 acagcccgcg agcgtattgc acagttgctt gacgaaggta gtttcgaaga actggatatg
     2161 tttgttcaac acagatgtac caatttcgga caagagaaaa aacatttcct cggcgacggt
     2221 gtggtaaccg gttatggaac gatagaaggt agattagtat atgttttcgc acaagatttc
     2281 acagtattcg gtggttcact gtcagaaacc atggcacaaa agatctgtaa agtaatggat
     2341 atggccatga agatgggtgc ccctgtcatc ggtatcaacg actcgggtgg cgcacgtatt
     2401 caggaaggca tcaacgccct gtccggttat gctgaaatct tccagcgcaa catcatggct
     2461 tcgggtgtca tcccacagat ttcaggtatt ttcggtccgt gtgccggcgg tgcggtttac
     2521 tctcccgccc tgacggactt cacgctgatg acggaaggta catcttacat gttcctcacc
     2581 ggaccggctg ttgtgaaaac agtaacgggt gaagacgtgt ctcaggaaga tctgggtggt
     2641 gcaagcgtac atgccagcaa gtccggtgta actcacttca ctgccgaaac cggtgaagaa
     2701 ggtctggcga ttattcgcaa gcttctcagc tttattccgc aaaacaacct ggaagaagct
     2761 ccattggtga actgtaccga ccccatcgac cgtatggacg acctgctgaa cgagatcatc
     2821 cccgacagcc cgaacaaacc gtacgatatg tacgaagtga tcggcgccat catcgataac
     2881 ggagaattcc tggaagtaca gaaagactat gcgaaaaatc ttattatcgg ttttgcccgt
     2941 atgaacggac aatcggtagg tgtggttgcc aatcagccta aatacctcgc cggagtactc
     3001 gacagcaatg cttcacgcaa aggtgcacgc ttcgttcgct tctgcgacgc attcaacatt
     3061 ccgctggtga cattggtaga cgttccggga ttccttccag gtacgggtca ggaatataat
     3121 ggtgtaatcc tgcacggagc caagttgctg tacgcttatg gtgaagccac tgtgcccaaa
     3181 gtaactgtta ccctgcgtaa atcttacggt ggttcgcaca tcgtaatgag ttgcaagcaa
     3241 ctccgcggcg acatgaacta tgcatggccg acagccgaaa tcgccgtaat gggcggtgcc
     3301 ggtgcagtag cggtattgta tgccaaagaa gccaaagatc aggaaaaccc tgctcaattc
     3361 ctggcagaca aggaagccga gtacactaaa ctgttcgcca acccatacaa tgcagccaaa
     3421 tacggttaca ttgacgacgt tatcgaaccg agaaacactc gtttccgcgt gatccgcgcc
     3481 ctgcaacagc tgcagaccaa aaaattaacc aaccctgcta aaaagcacgg taatattcca
     3541 ttgtaacaaa ttaaacatcg attgttatga ataagaaaag aatcgggata tttatcgctc
     3601 tgctggcaat ggtttgcatg gggctcaggg cgcaaagtgc caccagtctg agaatcaatg
     3661 aggttttggt cgtcaacgac cagaactatc aggatgacta cggactgcac aatgcgtgga
     3721 tagagatatt caacacctct ttcgcttctg taaatctgga aggttgcttt ctgacgaacg
     3781 ataaaaacaa tccgaccaaa tatcctattc ccaaaggcga cgtgctgact ctgatcaagc
     3841 cgcgccaaca tgctttgttc tgggcagacg gaatgcctaa ccggggtacg ttccacgtga
     3901 actttacgct cgacccgaac aaagagaatt acattgcttt gtatgactct aacggtaaaa
     3961 ctctgattga cgaagtaacc atacccgccg gtcagcttgc cgaccgttca tacgctcgcg
     4021 aaaaagacgg aagtgccaac tgggtagtaa aaggtgaagg agaacacagc tatgtcactc
     4081 cgagcaccaa caatatgact atcgacaaaa acccgaagat cgagaacttc aagaaacacg
     4141 actctatcgg tatcggtatg gccatcatcg ccatgtcggt cgtattcata ggtttggtac
     4201 tcttgtatct ttcattcaag gcggtaggta acgtagctgt cagactgggt aagaaaaacg
     4261 cgatgaaagc caccggtatc accgacaaga cggaagcgaa ggaaaagaac ctgggtagcc
     4321 acacaggtga agaaaccgcc gcgatcgcca tggctttgca tgaatacctg aatgacgctc
     4381 acgacgttga agacatgatt ttgactatca acaaagtgaa acgcacctac tcaccctgga
     4441 gttcgaagat ctacacactg cgtcagactc cgaaaagata attactaacc ctaataaaat
     4501 gaagcaatga aacaatataa atataaaatc aacggcaacc tttacaacgt tacagtgaac
     4561 gatgtagaag ataacatagc caacgttgaa gtgaacggaa cctcttataa agtagagttg
     4621 gacaagcccg tcaaagccgc tccgaaaacg gtgacccgtc cggcagccgc tccgaaaacg
     4681 gaaacaggtg ctccggtagt aaccaaacaa ccgacagctt ccaaaaaaga cggtgtgaaa
     4741 tctccgcttc cgggcgttat cctcgacata aaagtgaaag aaggggatac cgtgaagaga
     4801 ggccagacga tcatcatcct tgaggctatg aagatggaaa acaacatcaa tgccaataaa
     4861 gacggaaaag tagcagaaat taaagttaat aaaggagatt ctgtacttga aggtacagac
     4921 ctcgtaatca ttgaataatt atgggagatt tcgtatcatt cttagggaat aaccttgttg
     4981 acttctgggg atacaccggt tttgcgaacg cgacacccgg acatcttttc atgttgctaa
     5041 tcggtctctt ctttatctac cttgccgtag ccaaagaatt tgaaccgatg ctgctgatcc
     5101 ccattggctt cggtattctg atcggtaata ttccattcaa tatggaagcc ggattaaaag
     5161 tgggtattta cgaagaaggt tcggtactga acatccttta tcaaggagta acctccggct
     5221 ggtatccgcc actcatcttt ttgggtatc
//
