LOCUS       c00145_gnlPro..         5229 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AFNAMJJP_145.
ACCESSION   c00145_gnlPro..
VERSION     c00145_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:55:41
            Original ID  :: gnl|Prokka|AFNAMJJP_145
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5229
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5229
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1747:5227]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1748..5227
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5229
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5229
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(3..80)
                     /locus_tag="ctg145_1"
                     /transl_table=11
                     /translation="MKILLVTRGSQGDVLPYLAIAAELER"
     CDS             complement(114..1325)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg145_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.9e-17, bitscore:
                     51.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPDRIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQIEDFMTWVYGRFRTSTHTIEAARGMTRDDGVKILTESTL
                     RKIQDDISPIYSAYADRMVADTTGLRKPITRFFYLGLIRTLYNVRASNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNIKEYGCDISKVMNSEAMKQEIAAIGHGYKANCW
                     CTHGCWITSSVIFNPRKMIRSVYKGYRETKRLNHPLAINEQKLQTMEAKYHLDIERLRQ
                     LNIR"
     CDS             1601..1711
                     /locus_tag="ctg145_3"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             complement(1748..2503)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_4"
                     /sec_met_domain="Fer2_3 (E-value: 6.7e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.3e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.4e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(2534..4477)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 194.5; E-value:
                     1.1e-59)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.1e-60, bitscore:
                     194.5, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5e-39, bitscore:
                     122.0, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEINSKIPQGPLAEKWTNYKNHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQIGLGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERYAAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHI
                     MWEHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     EFVVPQTRNYKK"
     CDS             complement(4526..5227)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_6"
                     /sec_met_domain="SucD_FumR (E-value: 6.4e-75, bitscore:
                     239.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YYLMWLLNSSIGKKLIMSISGLFLVLFLLFHLSMNVAAVFSGEAY
                     NMVCSLLGSNWYAVAATLVLAAGVVIHFVYAIILTLQNRKARGNDRYAINARPKGVEWA
                     SQNMFVLGLIVILFMLLHFSQFWYKMMFAELIGHHEVALGSAMVSPQDGAAFINYYFQG
                     NAVITVLYLIWYVALWFHLTHGFWSAIQTIGWNNTIWMNRWECISKIVATVICGLFAII
                     TIIFFLNGVGA"
ORIGIN
        1 cgtctttcca attcggccgc tatggccaaa taaggaagga cgtcgccttg tgagcctctg
       61 gttactaata gtattttcat gctacgttta tttatgggct atgagggtct tgtttaccgg
      121 atattcagtt gacgcaaacg ctcaatgtcc agatggtatt tggcttccat cgtttgtagt
      181 ttctgctcat tgatggctaa gggatgattc aatcgtttcg tttcccggta tcctttatat
      241 acggagcgaa tcatcttacg tggattgaaa atgacggatg aggtgatcca gcagccgtgc
      301 gtacaccaac agttggcttt atacccatgt ccgatagcgg ctatttcctg tttcatcgct
      361 tcgctattca tcactttact tatatcgcaa ccatactctt ttatatttcc gagcggttcc
      421 cgtagctcgc aggcacgaaa acgtccgtcg tagtcgatta ccaaggtagt ctctccggcc
      481 gtacaatcca tcccccaagg ggtcgggtga tctatgttgc tggcacgcac attatataag
      541 gtacgtataa ggccgagata gaagaagcgt gtgatcggtt tccttaagcc ggtggtatcc
      601 gctaccatcc gatccgcata agctgaatag ataggggata tgtcatcttg tattttgcgt
      661 agcgttgact cggttaggat tttcacgcca tcgtcacggg tcatgcctct ggcagcctca
      721 atcgtatgcg tggaggtacg gaaacgtccg tatacccaag tcatgaagtc ttctatctgg
      781 tctatattgt atttcgtgat aacggttgct atatttttta agactttgcc gtcatctccg
      841 aaattctcct gtatcttacg cagggtttcc aaggccttat agaagtttcc cggtacgcct
      901 ctctgtgtat cgtgggtcgt accgaaacca tccaaggaaa ggcttacgtt aatattacaa
      961 tccgggcagt tctggcggaa ccgttttacc cattcgacga tccggtcagg ttttaagccg
     1021 ttcgtaggga tgttcacatc tttgatatgg ttgtttttgt agaataactc caatatttcc
     1081 ggtagatcct cacgcaaggt aggctcaccc ccggatatcc ataatcgatt gaattgcccg
     1141 gcggtttctg atatcttctt gatttcatcg aaagtgaggt cgggttgctt cttatccatt
     1201 tcttgggcat agaaacacat tgcgcatttg gcgttgcatc ggcctgttac gaaaaggaag
     1261 atcgactcga gttttctttc cgtgctcatt gccctgaaag aactggtggt tcgttttttg
     1321 tccattttat ttttgtttta ccatttgtct tatctggcgc atgatttttt ccttgtgttt
     1381 gatcaacacc cggagaaaat aacataagaa gccaaataaa atacttatta gcatatacta
     1441 tatgatcttt aaaggcaaag atcgacgata aaatgacgct aaacaatctc tatttgcagt
     1501 attttatggt ccatgtttaa aaggtaggtc tataaacaat taaagcctgt ctcacgacaa
     1561 gccctaacca aactttgtta accttaaatc taatactatt atgaaaaaac cacgatgcaa
     1621 atatacggac ttttcaattc ctgtcaaggg ttataatgct aaattatgtt ttacgacata
     1681 taacaaaaac aaaaaggcgc ctttctttta gagaaagacg ccttttccat gatatactaa
     1741 tcaaatatta gtcttgtaat ttagcgcaaa tgaactcgcg gttcaaacga gcgatgttgc
     1801 taacggagat attcttcgga cattccatct cgcaagcacg tgtgttcgta cagttaccga
     1861 agccaagctc atccatttta gcgaccatag ctttggcacg gcgagcagcc tcgactttac
     1921 cttgaggcaa caaagccagc tggctaacct tagcggatac gaataacata gcggaaccat
     1981 tcttacaagc ggcagcacaa gcgccacaac cgatacaagc ggcagcgtcc atggctaagt
     2041 ctgcgtcctt ctttgggata ggtatcgcat tagcgtcggg tacaccacct gtgttgacag
     2101 atgtgaaacc acccgcttgg atgattttgt cgtaagcgta gcggtctacc atcaagtcgc
     2161 ggataacagg gaaacctgcg gaacgccacg gttcgatcgt gatcgtctct ccatcgtgga
     2221 aacgacgcat atataactgg caagtcgtag ctccagttgc cggtccgtgc gggtgtccgt
     2281 ttacatacaa tgaacacatg ccacagatac cctcgcggca gtcgtgatcg aatacgatcg
     2341 gttccttacc ctcgttgacc aactgctcgt tcaagatatc caacatttcc aagaaagaga
     2401 cactctgatt gatcttctca attttgtatg tctcgaactg tcctttctcc ttcggacctc
     2461 tctgacgcca aaccttaagt gttacactta tatctttatc catgattcta attttttctg
     2521 agattgatga ttattacttc ttgtagttac gtgtttgagg aacaacgaac tcatagttca
     2581 aatcctcttt caacatgaca ggatctttgt cctcgccttg gtactcccag caagatacgt
     2641 acatgaactt atcgtcatga cgcaaggcct caccttcttc tgtctggtgc tccgtacgga
     2701 agtgaccacc acaagactca gcgcggtcca aagcatcgtg tgccatcaat gtaccgatct
     2761 cgataaagtc ggctacacgt aatgctttct ctaactcggt attcaagttg tctgcgttgc
     2821 ctgggatgaa tacgttgctc cagaactctt tcttcaagtc tttcagcatc tggatagctt
     2881 cttgcaaacc ttttgcgtcg cgtgccatac cgatatgttc ccacatgata tgacctagtt
     2941 tgcggtggat agtgtctacg gtttctttac ccttgacgtt catcaatttt tggatacgat
     3001 ccttgatggc cttttctgcc tcgacgaact ccggcagatc cgtgctgaag cgaggaactt
     3061 ggatttggtc ggacaaatag ttctggatcg tgtatggtaa tacgaaataa ccatcggcca
     3121 atccctgcat caaagcggaa gcacctaaac ggttcgctcc gtgatcggag aagtttgcct
     3181 caccgatagc gaacagaccc ggaatggagg tcatcaactc atagtcaacc cacaatccac
     3241 ccattgagta gtgcaatgcc gggtagatca tcatcggagt ctcgtatgga ttatcgttcg
     3301 tgatctcttc gtacatatcg aacaagttac cgtatttctg cttaacgacc tctttaccca
     3361 agcgattgat cgcgtcggag aaatccaaga atacggccaa gcccgtgctg ttcacgccga
     3421 aacctgcgtc gcaacgctct ttagcggcac gagaagccac gtcgcgagga accaagtttc
     3481 cgaatgccgg ataacgacgc tccaaataat aatcacggtc tgcctcggga atatctttac
     3541 ctttcttcgt tccggcctgt agagctttag cgtcttccag tttcttcgga acccagatac
     3601 ggccgtcgtt acgcaaggat tcggacatca aagtcaattt agattggaag tcacctttca
     3661 ccggtacgca agtcgggtgg atctgaacca tacaaggatt agcgaagtaa gcgcctttct
     3721 tgtagcattg ccatgcggcg gaaccgttag atgccatagc gttggttgac aagaagaacg
     3781 tattaacgta accacccgta gctacaacta cagcgtgagc ggcgtaacgc tcgatcttac
     3841 ctgtaatcaa gttgcgggcg atgataccac gagcacgacc gtctacctta actacatcca
     3901 acatctcatg acgagtgtac atcttaacct tacccaaacc gatctgacgg ctcaacgctg
     3961 aataagcgcc taacaaaagc tgctgacccg tctggccacg ggcgtagaac gtacgcgata
     4021 cctgagcgcc accgaatgaa cggttatcca acaaaccacc atattcgcga gcgaaaggaa
     4081 cgccttgagc cacgcactgg tcgatgattg agttagaaac ctcagccaaa cgataaacgt
     4141 tagcctcacg agcacggtag tcaccacctt tgatcgtatc gtagaacaaa cggtaaacag
     4201 agtcaccatc attctggtag ttcttagcgg cgttgatacc accttgagcg gcgatagagt
     4261 gcgcacggcg gggagaatct tggatacaga agttcaatac gttgaatccc atctctgcca
     4321 aagatgcggc agcggaagct ccggccaaac ctgtaccgac tacgataaca tccaaacgac
     4381 gcttgttagc ggggttcacc aatttctggt gatttttata attagtccac ttctcagcca
     4441 acgggccttg aggtatttta gaatttattt cagacataat gatttaattt caaaagttct
     4501 taattataaa acctgtagct tgcaattaag cacctactcc gtttaggaag aagatgatag
     4561 taatgatcgc gaaaagtccg caaataaccg tagctacgat cttagagata cattcccaac
     4621 ggttcatcca gatcgtatta ttccaaccaa ttgtttggat agcgctccag aacccgtgag
     4681 tcaagtggaa ccacaaagcc acgtaccaga tcaaataaag aacagtgatc acggcattac
     4741 cttggaagta atagttgata aaagcggctc cgtcttgtgg agataccata gctgacccca
     4801 atgcaacttc gtgatgaccg atcaattcgg cgaacatcat cttataccag aactggctga
     4861 agtgcaacag catgaacagg ataacaatca aacccaaaac gaacatgttc tgagatgccc
     4921 attccacgcc tttcggacga gcgttgatag cgtaacggtc gttaccacgt gccttacggt
     4981 tttgcaaggt aagaatgata gcgtacacga agtggatcac aacgcctgcg gccaatacga
     5041 gagtagctgc cacagcgtac caattagagc ccaacagcga acaaaccatg ttataagcct
     5101 ctccggagaa aacggccgcc acgttcatcg acaggtggaa caataagaac agaacaagaa
     5161 agaggcctga tatactcatt atcagcttct tcccgataga agaattaagt aaccacataa
     5221 gataataat
//
