LOCUS       c00002_gnlPro..         6588 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MFHNKMOJ_2.
ACCESSION   c00002_gnlPro..
VERSION     c00002_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-04 07:59:11
            Original ID  :: gnl|Prokka|MFHNKMOJ_2
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6588
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6588
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2856:6346]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2857..6346
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6588
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6588
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(3..122)
                     /locus_tag="ctg2_1"
                     /transl_table=11
                     /translation="MYLCLNNEFGIRYDRFIRALMNHGRNDGFVPLPFTGCDSY"
     CDS             160..867
                     /locus_tag="ctg2_2"
                     /transl_table=11
                     /translation="MNKLLSTLILSVGMSLSATAATSLLDSSIVGYSDFASQTADFGTL
                     KDSVSITSDDSNLTLSGITFNGYGANNRDVMSVTMVLDLSKINTPDAYTALFNAKNSSV
                     SWGVGLNTDRKLQGLWNNTSYSGPVVDTPLGTEGSLTISVVTGDLGTRIYFGDSTTYYS
                     NSGLKFGNQQIDQILMDAGLAESIEQLYIHDSLLSQEQVGQLMSEIASIPEPATASLGL
                     LGLAALMMRRRRA"
     CDS             1299..1532
                     /locus_tag="ctg2_3"
                     /transl_table=11
                     /translation="MNLQKQQLLLRLFRYFKSRKGEPRFFNKRNICLLAAAGAYILTPD
                     EFLFDGIGRLGLLDDAAVILGAVLTVFFPDGD"
     CDS             complement(1566..2351)
                     /locus_tag="ctg2_4"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSADGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFARDVMKLIPEFIDRPNVLGIGEIGLNRNT
                     SHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRPDLDPCRVLVDHCE
                     EHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANSAADWGDSDPLALTA
                     LACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     CDS             complement(2384..2656)
                     /locus_tag="ctg2_5"
                     /transl_table=11
                     /translation="MKRSKKEVLDADFAATRAGVLDVAAFLDRVDRGEGEADFRYHALM
                     DVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK"
     CDS             2857..3558
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_6"
                     /sec_met_domain="SucD_FumR (E-value: 3.3e-14, bitscore:
                     42.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             3580..5538
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_7"
                     /sec_met_domain="FAD_binding_2 (E-value: 5.4e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.5e-38,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             5579..6346
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg2_8"
                     /sec_met_domain="Fer2_3 (E-value: 2.9e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 9.6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 5.5e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIASGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 ttgtaactat cgcaaccggt gaatggcagg gggacgaatc catcgttcct cccatgattc
       61 atcagcgcgc gaatgaacct gtcatagcga attccaaatt cgttattcaa gcacaagtac
      121 atacattgaa tcttgcatca ttttattaag atactgacca tgaataaatt attatccacc
      181 ctgattcttt cggtcggcat gagcctaagc gctaccgcgg ctacctctct gctggatagc
      241 tccatcgtgg gatattcgga ctttgcctcc caaacggcgg atttcggcac cctgaaagat
      301 tccgtttcca tcacgtctga cgacagcaat ctgacgttat ccgggataac cttcaacgga
      361 tacggtgcca acaaccgcga cgttatgtct gtgacgatgg ttcttgacct gtccaaaatc
      421 aacactccgg acgcctatac cgctctgttt aacgccaaaa acagcagtgt aagctggggc
      481 gtgggcctga acacggaccg gaaactccag ggattatgga acaacacctc ctacagcggg
      541 cctgtcgttg acaccccgct gggcacggaa ggttccctga ccatttccgt agtcaccgga
      601 gatcttggca cccgcatcta ttttggagac agcactacgt actattccaa cagcggcctg
      661 aaattcggca accagcaaat tgatcagatt ctcatggacg caggactggc tgaatccatc
      721 gaacagctct acattcacga ctccctcctt tcccaggaac aagttggtca actgatgagt
      781 gaaatcgcca gcatcccgga acccgccacg gcttccctgg gcctgctggg actggccgcg
      841 ctgatgatgc gccgccgcag ggcataagcc tgtaccgtcc gcctccctat attcaaaggc
      901 cggctcacaa aatcgtgagc cggccttttc cttatccgtt caaacatctg ccggaaaaaa
      961 caggggggaa gaagcttcca tttgctgcgg taccctcacg gccagcactt gacggggaaa
     1021 tggcattcag gctcccccgg gagggtcatt tcaagagctt ccggccccgc cccacttccg
     1081 tacacgcccc tttttcccat gccgggacag ccggaaccgg agcatgcaaa gcccggcttc
     1141 cctcagcatc cggaacagcc catcctttca gcaatccatt tccccgcacg gtccggatcg
     1201 ctgcggtttc cgtccgtcgg caacagactc cttccccgaa acagggaaaa ggagtgggaa
     1261 aatggctgag cttgaacctt tatcccccgt tcccgtccat gaaccttcaa aaacagcagc
     1321 tgctactgcg cctgttccgg tatttcaaaa gcagaaaagg agaaccgcgt tttttcaaca
     1381 aacggaatat atgccttctg gccgccgccg gcgcctacat cctgactccc gacgaattcc
     1441 tgtttgacgg aatcggcagg ctgggccttc tggatgacgc ggcggtcatc ctgggcgccg
     1501 tcctcaccgt ctttttcccg gacggggact agagggccgt accctccgcc gcgcctcggg
     1561 cggaattagc gggacgacac tttccggaac ttcggggact gtcccatgaa tgtctccgga
     1621 ttttccagga gaagacgttc cgtttcctgc cgggaaaaac cgcgcctgcg tagctcgcag
     1681 gccagggccg tcagcgccag gggatcggaa tccccccagt ccgccgcgga attggcccag
     1741 acacggtcca ttccgtacaa ttccagaata tccgccgccc gcttgggatt cagcttactg
     1801 gtgggataaa gggtcagccc ggcccagtaa ccggcctcct tcaccagcgg gaaggtatgt
     1861 tcctcacagt gatccaccag cacgcggcaa gggtccagat ccggacggtt tttcagggcg
     1921 gaaagaatca ttacggtccc cttaagcttg tcttccagat ggggagtgtg aatcagcaca
     1981 agctggttga ggcgcaccgc cagatccaga tgctcttcaa aaatagtcat ttcatgggaa
     2041 gtattccggt tcagccctat ttccccgatg ccgaggacat tgggccggtc aatgaactcc
     2101 ggaatcagtt tcatcacatc ccgcgcgaaa gccacgtcct gcgcctcctt gggattgatg
     2161 cacagccatg tgtaatggtc catgccgaac ttggccgccc tggcgggttc gcattccgta
     2221 agctgtacga aataatcccg gaaaccgtcc gcggacgaac ggtcgtaacc ggcccagaac
     2281 gccggttccc ctaccgccag gcagccggcc tgcgccatgg cccggtattc gtccgtggtg
     2341 cggcacacca tatgtatatg aggttctatg aaagccatca ttcttatttg ctgaggttcg
     2401 gggctccgca ggccgctttg gctgcggcgg tttccggcac ggtgtcatcc ctgtaggaaa
     2461 gggcttccag aatggcccgg acgcgtccgg ggccggatgc ggacaagagg ggaagaacat
     2521 ccatcagggc atggtagcgg aaatccgcct ccccttcccc gcggtccacc cggtccagaa
     2581 atgcggccac atccagaacg ccggcgcgcg tcgctgcgaa atccgcgtcc agcacctcct
     2641 ttttacttct tttcatgcac agacgatacg cctatgcgga aagcctcgtc aagccggaac
     2701 aggcaacaaa atgataacca ttccaaactg ataaacagca acctctttcc ccggtttttc
     2761 aaaaatccga aaagcgccgg aaattcaaga agaggcttgc catccgggct tgaaccgggc
     2821 atcctgtgaa gacgcattta tttcattaga gcgcctatga atgcactagt aaaaaccata
     2881 tgtacattcg tgacatcctc catcggccgc aaaatcattg tggctctgac ggggttatgt
     2941 ttggtcttgt tccttgcggg acacctggca ggcaaccttc tcatcttcgg aggccctgaa
     3001 tggatcaaca cctacgccca cggcctgcac tccatgccgg aagcagccct ctggggaatc
     3061 cgcgcggggc ttgcggtgat cttcatcatc cacgtctggc tgaccatcca gctgaagctg
     3121 gaaaaccatg cggcccgcga accctacgtg ttcaagaaca ccattaaggc aacgctctcc
     3181 tcccgctaca tgatttacac cggtttgacc gtcctggtgt tcctgatcta ccacctgtac
     3241 cagtacaccc tgcgcgtggg atacgacccc gcccagttca ccacctttat ttccgacggt
     3301 acggtggaaa cctttgacgt gtacaagatg atcgtgaccg gattctccaa cgtatggtgc
     3361 tccgccttct acatcctggc cgttctgatg ctgttcagcc atctgcgcca cggggtacag
     3421 tccatcttcc agactgtggg cgtcgattcc cgcaaaatcc ggcccttcta caactttatc
     3481 gccatcgcct acggcgcgat tatctgcctg ggcttcatct ccgtgccggt ctcggttctt
     3541 ctgggtatca tcaaataacc atcaactcct ctcaaagtca tgattaattt tcccgtaagc
     3601 gactggatgc cggatgccaa aattccctcc ggccctattc aggacaaatg gtccaagtac
     3661 aaacttgagt ccaaactgat caatccgaac aacaagcgca agtacacggt acttgtggta
     3721 ggttccggcc tggctggcgc ctccgctgcc gccacgcttg ccgaacttgg ctacaacgtc
     3781 aaatgcttct gctaccagga cagcccccgc cgcgcccact ccattgcggc ccagggcggc
     3841 attaacgcgg ccaagaacta ccagaacgac ggcgactccg tttaccgcct gttttacgac
     3901 accgttaaag gcggcgactt ccgcgcccgt gaagccaatg tctaccgtct ggcggaagtc
     3961 tcctccaaca tcattgacca gtgtgtggcc cagggcgttc ccttcggccg tgaatacggt
     4021 ggcctgctgg acaaccgctc cttcggcggc gcccagctca agcgaacctt ctacgcacgc
     4081 ggacagacgg gccagcagct cctgctgggt gcctaccagg ccctggaaaa ggaagtggcc
     4141 aagggacaca ttgaaatgtt ctcccgccat gaaatgatgg acctggtgtt ggtggacggc
     4201 gaagccaagg gcatcgtcac ccgcaatctg gttaccggcg aaatcaaatc ctacgcaggg
     4261 gataccgtcc tgctctgcac gggcggctac ggcaacgtgt tcttcctgtc caccaacgcg
     4321 atgggttgca acgtcactgc cgcctacaaa gcctacaagc gtggtgcctt cttcgccaac
     4381 ccctgcttca cgcaaatcca ccccacctgc attccggtca aaggcgacta ccagtccaaa
     4441 ctgacgctga tgtcggaatc cctccgcaac agcggccgca tctgggtgcc caagtcccgt
     4501 gaaaccgcag aaaaaatccg taagaaggaa atcaagcctt ccgatgttcc cgaagaagaa
     4561 cgggactact acctggaacg caagtatccc tccttcggca acctggctcc gcgcgacatt
     4621 tcctcccgcg ccgccaagga agcctgcgac gacggccgcg gcgtggccgt caccggcaag
     4681 ggcgtcttcc tggattttgc ggatgaaatc aagcgcatgg gccgcgaatg gattgacggc
     4741 caatacggca atctgtttga catgtacgaa gaaatcacgg acgacgaccc ccatgaaacg
     4801 cccatgatga tctaccccgc ctcccactac accatgggcg gcctgtgggt ggactacaac
     4861 ctcatgtcca acgtgaaggg gttgcacgtc ctcggtgaag ccaacttctc cgatcacggc
     4921 gccaaccgcc tgggagcctc cgccctgatg cagggcctgt ctgacggcta cttcgtcatt
     4981 cccgccacgc tgcccgctta cctgacgacc accaagcccg gtgccgtcac caccgccgcc
     5041 ccggaattca aggctgcgga agacgaagtg aaggaacgca tcaacaagct cctgaatgtg
     5101 aagggcacca agacggtgga ctccttccac cgggaactgg gcctcatcat gtgggaagac
     5161 tgcggcatgg cccgcacgga agaaagcctg acccacgcca ttgaacgcat tccccagctc
     5221 cgcaaggaat tctgggaaaa cgtgcgcgta accggcacgg cggacggcat caacgcggaa
     5281 ctggaaaaag cctgccgcgt ggccgacttc ctggaattcg cccagctgct ctgctatgac
     5341 gccctgaacc gccaggaatc ctgcggcgcc cacttccgcg tggaatacca gctgccggac
     5401 ggcgaagcca agcgcaacga cgatgaatac gcctatgtag ccgcctggga atacactggc
     5461 gtggacagcc tgcctgttct ccacaaggaa ccgctgacct tcgagaacgt gcaccttgcc
     5521 atccgcagct acaagtaaag atagccaata cattaacact caaaagaaag attataacat
     5581 ggctaaaaca ctcaatctca ctctcaaggt ctggcgccag gaaaaccggg aagctaaggg
     5641 ccgcattgag acctatgccg ccaaggacat tccggtggac gcctccttcc tggaaatgct
     5701 ggacatcgtc aatgaaggcc tggtcaagga aggcaaggaa cccatccatt ttgaccacga
     5761 ctgccgggaa ggcatctgcg gcatgtgctc cctgaccatc aacggcattc cccacgggcc
     5821 ggaacgccag gtgaccactt gccagcttca catgcgcaag ttcaaggacg gcgataccgt
     5881 ctggattgaa cccttccgcg cgaaggcttt cccaatcctg cgggacctga tggtggaccg
     5941 ctccgccctg gaccgcatca tcgcctccgg aggctatatt gacgtgcgta ccggttccgc
     6001 gccgaacgcc aacaacatcg ccgtggaaaa agtgaaggcg gacgccgcct ttgacgcggc
     6061 cgcctgcatc ggctgcggcg cctgcgtggc ctcctgtaaa aacgcctccg ccatgctgtt
     6121 cacctccgcc aagatcgccc atctcaatct gctgccgcag ggccagccgg aacgttccaa
     6181 gcgcgtcctg gccatgatga agcaaatgga agcggaaggc ttcggcaact gcacgaacca
     6241 gtatgaatgt gaagctgcct gcccgaaagg catcagcgtg gacaacatcg cccgcctcaa
     6301 ccgcgacttc atgcgagcca acgccaagga aggtctctgt ttctaaagaa gattccttct
     6361 taagacgctc tgtaaaaact cccggcggca tcggctgtcg ggagtttttt tcattatcag
     6421 gtttccttga accaggaata aaatatcaga cagttgcttc ctcatcgtcg cgcagcgtaa
     6481 aaaataaatg ttacaccgga aggttcccag taatccagga gaccgtactt ggaaatgccg
     6541 gacgaaacct aagcaggccc cgtccgacag cgacggcccc atctttct
//
