LOCUS       c00132_gnlPro..         6757 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LFCFAIKE_132.
ACCESSION   c00132_gnlPro..
VERSION     c00132_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 13:08:03
            Original ID  :: gnl|Prokka|LFCFAIKE_132
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6757
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6757
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[430:3410]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      431..3410
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6757
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6757
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             431..2671
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg132_1"
                     /sec_met_domain="Gly_radical (E-value: 8e-47, bitscore:
                     146.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.4e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENENWAQFKGETWKKEINVRDFIQNNYKPYNGDDTFLVPSSE
                     KTKKVWNKLTEMFKVEREKGVYDAETKLPQGIDTYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTDEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVPLYGTQILIEEKKRFQKRLDIQELTEEIIQSREEITEQIKAL
                     KAFEKMCAGYGFDVTRPAKDAREAVQFLYLAYLAAVKDQDGAAMSLGRTSTFLDIYIEK
                     DICEGKLTEEEAQELIDQFIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTKTSYRYLHTLYNLGPAPEPNLTVLWFNNAPDNWKNFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQYFGARANLAKCLLYAINGGRDEKSGVQVAPRFEPITS
                     EYLDYNEVMEKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMGLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVKVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVDITEKFM
                     EYLRQHQTYRKATPTQSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGA
                     VAALASVAKLPFQHAHDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNV
                     NVFDRDLLLDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             2688..3410
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.2;
                     E-value: 2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg132_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-36, bitscore:
                     114.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKS
                     GTPYRMEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGVTV"
     CDS             complement(3413..3718)
                     /locus_tag="ctg132_3"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCNACLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
     CDS             3994..4560
                     /locus_tag="ctg132_4"
                     /transl_table=11
                     /translation="MEPIINSQVPEFKVQAFQNGSFKTVSSEDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYGKFQEMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKIAEIHDNGIGRNADELLRKVEAAQFVASHP
                     NEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             4682..6247
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg132_5"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.2e-49, bitscore:
                     156.6, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSAMKDQLSGIFSGLVSQYVFDIQVAPDHESRQELIDLLGDVA
                     SCSDHLSCSVADGEGLQFTIVKDGMPTGIRFRAVPNGHEFTSLLLAVLNSDGKGKNIPD
                     ESICARVRALNGPIKLTTYVSLTCTNCPDVVQALNAMATLNSRITHETVDGAINQKEVE
                     AMKVQGVPSVFADGKLINVGRSDLGELLSKLEAQYGINEGGVDNSVEKPVKNYDVLIVG
                     GGPAGAASAIYSARKGLNVAVIAERIGGQVKETVGIENLISVPQTTGQQLANDLLTHIN
                     NYPVDILEHRRVDKVELDGSAKLLTTSTGERFSAPALIVATGASWRKLNVPGEADYIGK
                     GVAFCPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEK
                     AKSLPNVEVFLHSQSLEVLGNGDKVTGLRVKDRKTDAERVIDLDGIFVQIGLAANSGVF
                     RDVVETNKPGEIVIDAHCRTNVPGIYAAGDVSTVPYKQIIIAMGEGAKAALSAFEDRMR
                     GSIG"
ORIGIN
        1 taacgattga tactaaaaaa acaatactgt ctatcgtaag ggtggttagt gaaccaccct
       61 tacacaggca caatgacatt ttataatcaa ataattctag acaacctgcg tgaaaccgta
      121 tgtaagaggt tttcttgatc tacggtttca attaccctgc gacaggcgaa cgagcggttg
      181 gatttaatat taataatact aaagtaatga aattaaaagt tctactgctt ttccctctgt
      241 gaagaccgga cggcagacaa aaaacaggcc ccggcggtat tcgtactgcc ggggcttcgt
      301 ttttatttcc cttatcccta tttgtaggta ttttccccag tttaacatcc ctcgtccttt
      361 acgatccttt tcagataggg ggaaaagacg tatctttgca cgcctaaacc gaaatagaca
      421 agataagctt atgaagtttg aaaacgagaa ctgggcgcag ttcaaaggag aaacctggaa
      481 aaaggagatc aatgtaagag actttatcca gaacaattac aaaccttata acggagacga
      541 tacgttcctc gtaccctctt cggagaaaac gaaaaaagtg tggaacaaac taaccgagat
      601 gtttaaggtt gaaagggaga aaggagtata tgatgccgaa acgaagcttc cccaggggat
      661 tgacacatac ggacccggtt acatcgataa ggaaaacgaa gtaatcgtcg gcttgcagac
      721 agatgccccg ttgaaacgcg gtatcttccc gaaaggcggt atccgcatgg tcgaaaacag
      781 tctggaagcc tatggttatc atctggaccc gatgacaaaa gagatcttca ccaaatacag
      841 aaagacgcac aacgaaggcg ttttttcagc ctatacagac gagatgattg cggcacgtcg
      901 ttccgccatc attaccgggt tgcctgatgc ctacggacgc ggacgtatca tcggtgatta
      961 ccgtcgtgta cctctgtacg gaacccagat cctgatcgaa gaaaagaaac gtttccagaa
     1021 acgtctcgat atacaggaac tgacagaaga aatcattcag agccgtgagg agataacgga
     1081 gcagatcaaa gcgctgaaag catttgagaa aatgtgtgcc ggttatggtt tcgatgtgac
     1141 acgtccggca aaggatgccc gagaagccgt gcagttcttg tatctggctt acctggcagc
     1201 cgtaaaggat caggatggag cagccatgtc tctcggacgt acttctactt tcctcgatat
     1261 ctatatcgaa aaagatattt gcgaaggcaa gctgacggaa gaagaggcac aggaattgat
     1321 cgaccagttt attatcaaac tgcgtattgt ccgtttcctg cgtactccgg aatacaacga
     1381 cctgttctcc ggcgaccctg tttgggtgac tgaatcgctg ggcggacaag gtgttgacgg
     1441 ccgttcgctg gtgacaaaga catcttaccg ttatctgcat accttgtata acctcggccc
     1501 ggcacccgaa cccaacctga cggtgctttg gttcaacaat gctccggata actggaaaaa
     1561 cttctgcgca aaagtatcga tcgatacctc tgccatccag tacgagaacg acgatctgat
     1621 gcgtccggac tatggcgacg attacggtat cgcctgctgt gtgtctccga tgaagatcgg
     1681 taagcagatg cagtatttcg gagctcgtgc caatctggca aagtgcttgt tgtatgcgat
     1741 caatggcgga cgtgacgaga aatcgggtgt gcaggtggct ccccgtttcg aaccgatcac
     1801 cagcgagtat ctggattaca acgaagtgat ggagaaatac gagcagatga tgcgctggtt
     1861 ggcaaaagtc tatgtcaatg ctctgaagat catccactac atgcacgaca aatatgcgta
     1921 tgaagctttt gagatggggc ttcatgacgg agatgtggaa cgtatccgcg caaccggtat
     1981 cgccggcctt tctatcgttg ccgactcgct ggctgccatc cgtgacacga aagtgaaagt
     2041 gatccgtgac gaacgtggtt tggctgtcga cttcgaacgc gaaggcgaat atgtgccttt
     2101 tggaaacaat gacgaccgca cggacagtat tgctgtcgac attaccgaga agttcatgga
     2161 atatctgcgc cagcatcaga cataccgcaa ggcaactccg acacaatcta tcctgacgat
     2221 cacttcaaac gtggtttacg gaaagaaaac cggaacgact cccgacggcc gtccgggcgg
     2281 tacgccgttc gctccgggag ctaacccgat gaacggacgc gacacgaaag gggctgttgc
     2341 cgctttggct tctgttgcca aactgccttt ccagcatgcg catgacggta tctcctatac
     2401 atttgccgtt tcaccggcta cattgggtaa ggagcgcgat attcagataa ataatttggt
     2461 gagcctgttg gatggctatt ttacgcctga cggtggccag catctgaatg tgaacgtgtt
     2521 cgatagggac ttgttgttag atgcaatgga gcatccggag aaatacccgc agcttacgat
     2581 ccgcgtttcg ggctatgccg tcaacttcgt gaagctgaca cgcgaacagc agctcgacgt
     2641 catctcgcgc accatcaaca gcagtttata attgtccatt gattaatatg ttggggcgta
     2701 tacattcttt agagagtttc gggaccgttg acggtcccgg aattcgcttt gttgttttca
     2761 tgcagggatg tccgcttcgt tgtctgtatt gccacaatcc ggatacttgg gaggtaaaaa
     2821 gcgggacgcc ttatcgaatg gaaccggaag ccttgcttgc cgaagtgctg cgctataaga
     2881 attttatcgc taaaggtggt gttaccgtga ccggtggtga gcctttgtta caacctgaat
     2941 tccttaaaga gtttttccgc ctttgccgtg agaacggtat tcatacggcg ttggacactt
     3001 ccggctatat ctgttccggt aaagcattgg aggtgctcga acaggtcgat ctggttctgt
     3061 tagatatcaa gacgatcgac gccggtcttc atccccgttt gacggccgta aaactcgata
     3121 atactttacg ctttcttgat gaactggaga aacgcggcat ccctgtatgg attcgccacg
     3181 tgatcgtgcc cggcctgacg gacgatgatg aagccttgag caagctggcc gaatatatca
     3241 gttcatataa cgtggtccag aaagccgaac ttctgcctta tcatacaatg ggagcttaca
     3301 aatatgaggc acaaggcctg gactataagc tgaaaggtgt ggaaccgctt tcggcagaac
     3361 gtctggccaa tgcgaaagcg atctttatga aacacggagt aaccgtataa gcctaatcga
     3421 gtgtctgctc tttgatgatg atcggtttac ggctgatccg gttgactgtg cggacgctgt
     3481 cgctttcaat actggtaaca tctccctcgc agttggtgaa atagatgtaa tgtcccatat
     3541 ccatgaaacg atagacctta cacccttcgt gttcgaacag atattcgact tgataagtgg
     3601 aattgtttac cggtttttcc tttgtaaggg gtatttgtga gacacatgaa gaaagacatg
     3661 cgttgcaaac gagcaataga cttgctgaaa atgtattata caaactagct cttctcatct
     3721 tcgaatattt ttaagttaac atccaaagtt aacaattaaa gaatcacatg gtatgatcga
     3781 aaggtatttt acctacaatt aggtatattt ttattgttcc ggaaaacatt actttgcttc
     3841 ctattgtcat agtagataag tatgcttgca gatagaaatc cgtctatcgg cagataaaaa
     3901 ttatcaattt gaaaaattct ttcaatcagt atcttacgat ttacctttgt agggtaaaca
     3961 aaaatagtag taacaaatta aatagtaaaa atcatggaac caattatcaa ttcacaagta
     4021 cccgaattca aagttcaagc ttttcagaat ggtagtttca agacagtaag tagcgaagat
     4081 atcaagggca aatgggccat ctttttcttc tatcctgccg actttacgtt cgtatgtccg
     4141 accgaattgg tcgatatggc tgacaagtac ggcaaatttc aggagatggg tgtcgaagta
     4201 tattccgtaa gtacagactc gcactttgta cacaaagcat ggcatgatgc ttccgaaacg
     4261 atccgtaaga tcaaatatcc gatgctggca gacccgacag gggctttaag ccgtgcattc
     4321 ggtgtgatga ttgaagaaga gggtatggct tatcgcggta cgttcctggt aaatcccgag
     4381 ggaaagatca agattgccga aattcacgat aacggtatcg gccgtaatgc agacgaattg
     4441 cttcgtaaag ttgaagctgc ccagttcgtt gcttctcacc cgaacgaagt ttgtcctgca
     4501 aagtggaaaa aaggcgaagc aaccttgaag ccgagcatcg acttggttgg taagatctaa
     4561 aaagaatgat gtgccaattg attaatgtgc caatatgcca attatttatg aggtaataca
     4621 tcgctttagt cgattggtac attctttaat tggcacgtta ataaattgac atattatttt
     4681 tatgttagac tcagcaatga aagaccagct ttcgggtatt ttctccggac tggtatccca
     4741 atatgtattt gatatacaag tcgctcccga tcacgaaagc cgtcaggagc taatagacct
     4801 gcttggtgat gtggcatcat gctcggatca tttgtcatgc agcgttgcgg atggcgaagg
     4861 acttcagttt acaattgtaa aagacggtat gcctaccggt atccggtttc gtgcagtacc
     4921 taacggacat gagtttacat ccttgttgtt agctgtcctg aatagtgatg gaaaaggtaa
     4981 aaatataccg gacgaaagta tctgtgctcg tgtcagagcc ttgaatggtc cgatcaagct
     5041 gacgacttat gtttctctta cttgcaccaa ttgtccggat gtagtgcagg cactcaatgc
     5101 catggctact ctcaatagcc ggatcaccca cgaaacggtg gatggcgcta tcaaccaaaa
     5161 agaagttgag gcaatgaaag tacagggagt cccttctgtt tttgccgatg gcaaactgat
     5221 caatgtgggg cgtagtgatt tgggagaatt gctttccaaa ctcgaagccc aatacggaat
     5281 taatgaaggg ggtgttgata actctgttga aaaacctgtt aagaactatg atgtgttaat
     5341 agtggggggt ggacctgccg gtgcagcttc tgctatctac tcggcacgta aaggcttgaa
     5401 tgtcgcagtg atagccgaac gtatcggagg acaggtcaaa gaaacggtcg gcatcgagaa
     5461 cctgatttcc gttcctcaga ctaccggaca gcagttggcg aacgaccttt tgacacatat
     5521 caacaactat ccggttgata tactggagca tcgtcgtgtg gataaagtcg aactggatgg
     5581 atcggcgaaa cttctgacca cttctaccgg tgaacgtttt tctgctcctg cccttatcgt
     5641 ggcgacaggt gcaagctggc gcaagttgaa tgttccgggc gaagcggact atatcggaaa
     5701 aggtgtcgct ttttgccctc actgcgatgg tccgttctat aaaggtaagc acgtagcggt
     5761 tgtcggcggt ggaaattcgg gtatcgaagc ggctatagat ttggcaggca tttgttcgaa
     5821 agtgacggtt ctggaattta tggacgaact gaaagccgac caagtgttgc aggaaaaagc
     5881 aaagagcttg ccgaatgtgg aagttttctt gcattcccaa tctttggaag tgcttggtaa
     5941 tggcgataag gtgacaggtc ttcgtgtaaa agaccgtaag acggatgcgg agcgggtgat
     6001 cgacctggac ggaatctttg tccaaatcgg cttggcagcc aatagtggcg ttttccgcga
     6061 cgttgtcgaa acgaacaaac cgggtgagat tgtaatcgat gcgcattgtc ggacgaatgt
     6121 tcccggcatc tatgcggcgg gcgacgtctc gaccgtaccc tataaacaga taatcatagc
     6181 aatgggagaa ggggcaaaag cggctctatc tgcttttgaa gaccgcatga gaggttcaat
     6241 cggttaatat ttagtatttg ctatgaacga cgaaggtgtg caaattgaac tacacgaatg
     6301 tgtgttcaaa attgcacacc ttttcttttt tacgcacaaa accccgactt tcacaacagg
     6361 gcaaccgcat caaaattttc caaggttgtg atctccatac gaagttttct acaaatcctc
     6421 tatcactcta tcactacgaa ggttgtagct attaatattc aagcgttaga ggagtgatag
     6481 aggaaaacat cctgtatcac tgcctctatc actcctctaa caggacgttt ttatcatatg
     6541 attggaaacc aatatattgc cttcttttgc ctttgaaaaa gcatataaaa taatcgaata
     6601 aacaaatgta aaaacgaatg caataggtgt taaattgacg aaatacggtt cttcattcca
     6661 caaaagccct ctcttttttt agcagaaaca agcagataat tccgtaaaca cccccatgtt
     6721 gccatcctta acatccgggt gtagagagcc ggtacat
//
