LOCUS       c01103_gnlPro..         2370 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IGKKACOA_1103.
ACCESSION   c01103_gnlPro..
VERSION     c01103_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-06 13:51:42
            Original ID  :: gnl|Prokka|IGKKACOA_1103
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2370
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2370
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[872:2370]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      873..2370
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2370
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2370
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..640
                     /locus_tag="ctg1103_1"
                     /transl_table=11
                     /translation="GNGGGLPTNGSENFAPRPMTNRVDLMTIMNGAVSIPADQEVIFEE
                     GVPYLKSNTTAVMDYEHHQPVSFGLSVRKSLPKGFSVETGLTYTLLSSDIKRQGDTKMQ
                     SQKLHYIGIPVRGNWNFLEKKYFTLYVSAGGMVEKCVYGKLADDKVNVKPLQFSVAGAV
                     GAQFNATDHVGLYVEPGVSYFFDDGSKVQTIRKERPCNFNLQAGLRFTY"
     CDS             complement(873..1598)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.9;
                     E-value: 1e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1103_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.5e-31, bitscore:
                     97.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIEAKGG
                     TATDPEEIVRMAVSQKAFFGKKGGITFSGGEPTFQAKSLIPLFKRLKEKGIHICLDTNG
                     GLWNNDVEELLELTDLVLLDIKEFNPEHHQSLTGRSNEQTLKTAAWLETNHKPFWLRYV
                     LVPGYSDFEDDIRQLGEHLGTYQMIQRVEILPYHTLGVHKYEAMNKEYMLKGVKENTPE
                     QIEKAEKLFRQYFRTVQVN"
     CDS             complement(1603..2370)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1103_3"
                     /sec_met_domain="Gly_radical (E-value: 1.5e-48, bitscore:
                     151.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1.8e-64, bitscore:
                     205.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHDKYYYEKAQMAFVDTDPRINLAYGVAGLSIAIDSLSAIKYAHV
                     KARRNDIGLTEGFDIEGAFPCFGNDDDRVDHLGVDLVYFFSEELKKLPVYKNARPTLSL
                     LTITSNVMYGKKTGATPDGRAKGIAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDG
                     ISNTFSIVPKSLGATQEDRIDNLVTMMDGYFTKGAHHLNVNVLNREMLQDAMEHPEKYP
                     QLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 cggtaatgga ggtggacttc cgacaaatgg ttccgaaaat tttgcgcccc gtccgatgac
       61 taacagggtc gacttgatga cgatcatgaa tggagctgtg agtattcctg ccgatcagga
      121 agttatattt gaagaagggg ttccctatct gaagtccaat acaactgccg tgatggatta
      181 tgaacatcat cagccggtta gcttcggact gtctgtacgt aagtctcttc ccaaaggttt
      241 ctctgtagag acgggattga cttatacgtt gttgtcttct gacatcaaac ggcaggggga
      301 taccaagatg cagagtcaga aattgcatta tataggtatt cctgtacgtg gaaactggaa
      361 ttttctggaa aagaaatatt ttacactcta tgtatctgcc ggaggaatgg ttgaaaagtg
      421 tgtttatgga aagttggcgg atgataaggt gaatgtgaag cctttgcaat tttctgtagc
      481 cggtgccgtg ggagcgcaat tcaatgcgac agatcatgtc ggcctatatg tggaaccggg
      541 agtttcgtat ttctttgatg atggctccaa agtacaaacc atccgaaaag aaaggccttg
      601 taactttaac ttgcaggcag gattgcgatt cacttattaa agtagggtgg caaaaaatat
      661 gccggttaca ttatctccct gttcttgcct tgtattgtaa tgaattgagg caagaacagg
      721 gagattttta tgtcgtgact tacaaaatta taaataagcg caatcgtcat aattcaccgc
      781 agagtgaact taatctgttg attgccaatg aatctttatc tgtgtaaatt cgtgtaatct
      841 gtggcgaata cgaatttcga cacttatata agtcaattca cttgtacggt ccggaagtat
      901 tgcctgaata gtttctcagc cttttctatc tgctccggag tattctcttt cactcctttc
      961 agcatatatt ctttgttcat tgcttcatat ttgtgaacac ccaaggtgtg gtaaggcaat
     1021 atttctacac gctgaatcat ctggtacgtt ccaagatgct ctcccagttg ccggatatcg
     1081 tcttcgaagt cactgtagcc gggcactaat acgtatcgca accagaatgg tttatgattg
     1141 gtttcaagcc aggctgcggt tttcagggtc tgctcgttgc ttcttccggt taaagactga
     1201 tgatgttccg gattaaattc tttgatatct aataaaacca gatcagtcaa ttccaatagt
     1261 tcctctacat cattgttcca tagcccgcca ttggtatcaa ggcaaatatg gattcctttt
     1321 tcctttagtc ttttaaaaag tgggataagc gatttagctt gaaaagtagg ctctccgccg
     1381 gaaaaggtga tacctccctt tttaccgaag aaagctttct gactgacggc catgcgaact
     1441 atttcctcag ggtcggttgc tgtccctcct ttggcttcaa ttgtgtccgg attggcacaa
     1501 tagaggcaac ggaagttaca cccttgaagg aaaacgacga gccggagacc cggcccgtcg
     1561 aatgttccca ttgactcgta tgagtgtacg tttatcattc aattacattc tttcgtggaa
     1621 actgcggctg attacttcca actgatgttc gcggctcaat ttcacgaaat taacggcata
     1681 tcctgagaca cggatagtca gttgcggata cttttcggga tgttccatcg catcctgcaa
     1741 catttcccgg ttcagcacgt ttacgttcag gtggtgtgcg ccttttgtaa agtatccgtc
     1801 catcatggtc accagattgt cgatacggtc ttcttgtgtt gctcccaatg atttgggaac
     1861 gattgagaat gtattgctga taccatcctg tgagtcgcga taacgcaact tggctacaga
     1921 actcagtgag gcgattgcac catttttatc acgtccgtgc atggggttgg caccgggagc
     1981 aaaggcaatg cctttggcgc gtccgtcagg agtggcaccg gtcttcttgc catacattac
     2041 gttcgaagtg atagtcagca gcgagagggt aggacgggca ttcttatata cggggagttt
     2101 ctttaattct tcgctaaaga aatataccag gtccacaccc aggtggtcta cacggtcatc
     2161 gtcattaccg aaacaaggga aagcaccttc gatatcgaat ccctccgtca gtccgatgtc
     2221 gttgcgacgg gctttcacat gtgcatattt gatggcggac agtgaatcga tggcgattga
     2281 aagtccggct acaccataag cgaggttaat gcggggatca gtatccacga aagccatctg
     2341 cgccttttcg tagtaatact tatcgtgcat
//
